gold mine in the sky gene autry
Tài liệu Báo cáo khoa học: A genetic screen identifies mutations in the yeastWAR1 gene, linking transcription factor phosphorylation to weak-acid stress adaptation docx
... DNA-binding zinccluster at the N-terminus, whereas the acidic activationdomain is usually present at the C-terminus. The mid-dle homology region (MHR) bridging the DNA-bind-ing and the activation ... were raised in rabbitsagainst a GST-War1p fusion protein containing 294 aminoacids of the C-terminal part of War1p (amino acids 650–944) fused in- frame to the C-terminus of GST. The gene fusion ... lack of DNA binding. Indeed, in the case of War1-42p, in vivo footprinting data(Fig. 5) indicate an inability to bind to the WARE,which is normally decorated by wild-type War1p in the presence...
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Báo cáo khoa học: Evaluation of potential regulatory elements identi®ed as DNase I hypersensitive sites in the CFTR gene doc
... a proportionate increase in the intensity of the DHS fragments in the control samples. In Fig. 7A the forskolin-treated chromatin shows a relative increase in the intensity of the DHS10a (1716 ... similar intensity but the DHS in intron 18 isless evident ( Fig. 5B). In contrast, the DHS in intron 18 ismore prominent than those in introns 16 and 17 (Fig. 5A) in chromatin from Capan1 cells. The ... + 10 kb in intron 1 of the CFTR gene augments intestinal expression of the gene in vivo, both in human colon carcinoma cells and in transgenic mice [15]. It is probable t hat several other DHSthat...
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Tài liệu Báo cáo khoa học: "THERE STILL IS GOLD IN THE DATABASE MINE" potx
... and mine it very deeply, not necessarily discovering every aspect of the domain but requiring that the various aspects be integrated with one another to produce a coherent whole. Even in the ... officers represented in it. The system merely made no distinction between "all" and "any", and interpreted the final query to mean the But, you are probably thinking, those examples ... THERE STILL IS GOLD IN THE DATABASE MINE Madeleine Bates BBN Laboratories 10 Moulton Street Cambridge, MA 02238 Let me state clearly at the outset that I disagree with the premise...
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The Retinoblastoma Gene Family in Cell Cycle Regulation and Suppression of Tumorigenesis
... found in the A and B subdomains. The pocket-domain is responsible for binding to proteins containing LXCXE motifs while the pocket-domain and the C-domain are involved in binding E2F proteins. ... cellular proteins. Although the binding site for LXCXE motif containing proteins is present in the B sub-domain, the crystal structure of the pRb A/BpocketboundtotheLXCXE-containing part of HPV-16 ... pRb inhibits the transcriptional activityof E2F by binding to its carboxy-terminal transactivation domain, therebypreventing the interaction of E2F with the basal transcription machinery(Helin...
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Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt
... man-ganese in the transcriptional regulation of the nrdoperon [35] may not be considered in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo ... results) to the nucleotide-producer C. ammoniagenes [10]. The present strategyof reintroducing the nrdF gene into the geneticbackground of corynebacteria comprised an initialtransfer into the accessible ... samples. The rad-ical concentration was calculated by determining the areaof the radical signal in the absorption spectrum by cubicspline interpolation [52] in the range 403–413 nm using the coefficient...
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Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf
... containing the MuvB proteins Lin-37, Lin-52, Lin-54 and chromatin-associated Lin-9 and Lin-53 ⁄ RBBP4 ⁄ RbAp48.Furthermore, A-Myb and B-Myb were present in immunoprecipitations for Lin-9, Lin-37 ... complexes of cyclin-dependent kinases (cdks)and their corresponding cyclins. A prominent exampleof such a pair is cyclin B and cyclin-dependentkinase 1 (cdk1 ⁄ Cdc2) controlling the checkpointbetween ... appearsstronger in G2⁄ M than in G1[44]. This contradictsrepression through a complex in G1and in vivo foot-printing results in the original definition of the sites[10]. Taken together, the sites in...
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Tài liệu Báo cáo khoa học: Metabolic gene switching in the murine female heart parallels enhanced mitochondrial respiratory function in response to oxidative stress pdf
... results in the emission of light. The intensity of the bioluminescence was detected using aluminometer, and the mitochondrial ATP concentration wasdetermined. Mitochondrial protein concentration ... 2007)doi:10.1111/j.1742-4658.2007.06051.x The mechanisms underlying increased cardioprotection in younger femalemice are unclear. We hypothesized that serine-threonine protein kinase(protein kinase B; Akt) triggers a metabolic gene ... in fatty acid utilization,increase in glucose utilization) in the murine femaleheart. Moreover, our data suggest that these changesoccur in an Akt-independent manner. Further studiesare therefore...
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Tài liệu Báo cáo khoa học: Effect of deletion of the DNase I hypersensitive sites on the transcription of chicken Ig-b gene and on the maintenance of active chromatin state in the Ig-b locus docx
... and the remaining DHSs in the deleted cellswere examined to determine the roles of these DHSson the transcription of Ig-b gene and on the mainten-ance of the active chromatin state.ResultsGeneration ... determined bytransient transfection, was associated with DHSs in the Na channel gene and in the first intron of the Ig-b gene. Furthermore, the acetylation status of H3 andH4 histones was examined ... in the b-globin locusare the same as the wild type control [17]. Thus,there again has been no description either of the region involved in DNase I general sensitivity orof the region for the...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... from them, are the trueinducers of galA expression. This indicates that galA iscoexpressed with both the pectinolytic genes encoding mainchain cleaving enzymes as well as with the arabinanolyticgenes ... synergy of enzymes degrading the pectin side chains was studied [34]. GALA had a positiveeffect on the activity of the other enzymes involved in the degradation of these side chains.DISCUSSIONA. ... under the control of the xylanolytic activator protein [32,41]. The increase in expression levels of galA in the CREA mutant,indicates that at least one of the detected putative CREAbinding sites...
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Tài liệu Báo cáo khoa học: Characterization of the promoter for the mouse a3 integrin gene Involvement of the Ets-family of transcription factors in the promoter activity doc
... products in the absence of the cDNA library.Fig. 6. Transcription start sites in the integrin a subunit genes. The sequences flanking the transcription start sites in the integrin a subunitgenes ... binding factors. The introduction of mutation in one of the Ets-bindingsequences greatly decreased its promoter activity, suggestingthat the transcription of the a3 integrin gene in these cells ... includingEts, GATA, and MyoD/E-box binding factors. The introduction of mutation into one of the putative Ets-binding sequences suppressed the promoter activity. In addition, the specific binding...
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Tài liệu Báo cáo Y học: Analyses of the CYP11B gene family in the guinea pig suggest the existence of a primordial CYP11B gene with aldosterone synthase activity docx
... intron sequences requires the introduction of 11and 14 gaps for intron 2 and 5, respectively, as compared to1 (intron 7) to 7 (intron 6) for the remaining introns. Finally, the 3¢ UTRs of the ... pig genes. Interestingly, we observed the greatest differences between these closely related genes withinintron 2 and intron 5. This could indicate frequent recom-bination between the genes ... zone-specificbiosynthesis of mineralocorticoids. In this respect it wouldbe interesting to see whether expression of adrenodoxin isdifferentially regulated.To investigate the evolution of the CYP11B genes,...
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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx
... asSTARS-interacting proteins. These novel proteins con-tain four LIM domains and a C-terminal villin head-piece domain, which mediates actin-binding in severalproteins, such as villin and dematin ... occurring C-terminal 100 kDa fragment offilamin, interacting with the motor protein dynein,may exert its inhibitory effect by interfering with inter-actions between the N- and C-terminal domains, ... the AR transactivation function, and the minimal functional fragment of SV, which onlycontains one actin-binding site, is located in the nucleus, recruiting actin into the chromatin-remodelingcomplex...
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Báo cáo khoa học: Gene expression silencing with ‘specific’ small interfering RNA goes beyond specificity – a study of key parameters to take into account in the onset of small interfering RNA off-target effects potx
... differences in exposure times during western blot-ting. We also observed a slight increase in SREBF1protein level triggered in the presence of the NTsiRNA, in agreement with the slight increase in SREBF1 ... out an indirect effect resulting from the silencingof the transcription factor SREBF1, which wouldmodify gene expression in an siRNA-independentmanner. Therefore, these variations in mRNA ... cangenerate side effects, by inducing the degradation ofnontarget mRNAs sharing sequence homology with the siRNA seed region, or by repressing the translationof unintended proteins [5–10]. Indeed,...
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Báo cáo khoa học: Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebrafish (Danio rerio) pptx
... lipid-binding protein, intesti-nal 15 kDa protein, ileal bile acid-binding protein andgastrotropin, reflecting the speculations of authors onits intracellular function(s). In vitro binding assaysrevealed ... according to the tissue from which they were initially isolated, e.g. liver-type fatty acid-binding protein (L-FABP), brain-typefatty acid-binding protein (B-FABP), intestinal-typefatty acid-binding ... The coding sequence derived from the fabp6 gene at the Wellcome Trust Sanger Institute wasderived from the Tu¨bingen strain of zebrafish, while the sequences for the fabp6 cDNA generated in thisstudy...
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Báo cáo khoa học: The fabp4 gene of zebrafish (Danio rerio) ) genomic homology with the mammalian FABP4 and divergence from the zebrafish fabp3 in developmental expression pot
... CanadaFatty acid-binding proteins (FABPs) are encoded by amultigene family termed the intracellular lipid-bindingprotein (iLBP) genes [1,2]. In vertebrates, at least 16paralogous iLBPs, including 10 ... shown in upper-case letters, with the codingsequences of each exon underlined and the deduced amino acid sequence indicated below. Numbers on the right indicate nucleotide posi-tions in the gene ... nucleotide difference in the coding region of exon2, resulting in an alteration of the ninth codon of thisexon from GTT to GTC without changing the encodedamino acid. The genomic sequence and...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ mở máy động cơ lồng sóc hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25