glycosaminoglycans oligosaccharide analysis by liquid chromatography capillary electrophoresis and specific labeling

Analysis of keto enol tautomers of curcumin by liquid chromatography mass spectrometry

Ngày tải lên : 01/07/2014, 13:22

... and enol form by negative MS/MS because the number of exchangeable hydrogens of the enol form and the keto form are different Two phenolic hydrogens of keto form, and two phenolic hydrogens and ... Analysis of curcuminoids by positive and negative electrospray ionization and tandem mass spectrometry, Rapid Commun Mass Spectrom 20 (2006) 1001–1012 [18] F Payton, P Sandusky, W.L Alworth, NMR ... bioavailability of curcumin in rat and the herbal analysis from Curcuma longa by LC–MS/MS, J Chromatogr B 853 (2007) 183–189 [11] J Li, Y Jiang, J Wen, G Fan, Y Wu, C Zhang, A rapid and simple HPLC method...

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Novel methods for quantitative analysis and evaluation of effects of chronic exposure to microcystins by capillary electrophoresis and metabolomic studies

Ngày tải lên : 11/09/2015, 10:14

... microcystins Determination of microcystins and nodularin in the same run by high performance liquid chromatography (HPLC) has been reported and capillary electrophoresis (CE) can also be used for ... (as in gel electrophoresis) or can be a liquid, in most cases a buffer solution In capillary electrophoresis, the medium is contained in a small capillary 40 Therefore capillary electrophoresis ... difference between the inlet and outlet of the capillary, of the capillary, is the viscosity of the liquid and is the diameter is the capillary length For injection by gravity, (vii) is acceleration...

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Báo cáo khoa học: Angiotensin-converting enzyme inhibition studies by natural leech inhibitors by capillary electrophoresis and competition assay doc

Ngày tải lên : 23/03/2014, 12:20

... by ACE and its inhibition in a onestep analysis by capillary zonal electrophoresis Thus, we report for the ﬁrst time in invertebrate the existence of a novel ACE inhibitor, the LORF peptide and ... dried by speed-vac Finally, 30 lL sterile water was added on the pellet and peptides were analyzed by capillary zonal electrophoresis Samples (2 nL) were injected under vacuum into a PACE 5000 capillary ... °C and were terminated by addition of 1% triﬂuoroacetic acid (v/v) The internal standard FMRF-amide was added and samples were centrifuged at 20 000 g for 10 at °C Supernatants were collected and...

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báo cáo hóa học: " Method optimization and validation for the simultaneous determination of arachidonic acid metabolites in exhaled breath condensate by liquid chromatography-electrospray ionization tandem mass spectrometry" doc

Ngày tải lên : 20/06/2014, 00:20

... internal standard and b) without internal standard Figure the corresponded product the deuterated standards with Standard chromatogram of ion scans Standard chromatogram of the deuterated standards ... Leukotrienes LTB4 and LTE4as analytical standards and the labelled [2H4] LTB4 and [2H4] PGE2 as internal standards were purchased from Cayman Chemicals Company (Michigan, USA) All analytical standards ... [16] Liquid chromatography Liquid chromatography separation was performed on a Hewlett-Packard HP 1100 series HPLC system equipped with a binary gradient pump, an isocratic pump, degasser and...

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Báo cáo khoa học: "Simultaneous Determination of Various Macrolides by Liquid Chromatography/Mass Spectrometry" pps

Ngày tải lên : 07/08/2014, 15:20

... of drugs The m / z 576.5 and 679.5 in mass spectra of erythromycin and roxithromycin were corresponding with Simultaneous Determination of Various Macrolides by Liquid Chromatography/ Mass Spectrometry ... roxithromycin, azithromycin and clarithromycin in human serum by high-performance liquid chromatography using pre-column derivatization with 9-fluorenylmethyloxycarbonyl chloride and fluorescence detection ... adduct ions [M+Na]+ and [M+K]+, and several fragmentation ions (Fig 3) The molecular ions, [M+H]+ at m / z 734.5, 837.5, 494.4 , and 916.5 for erythromycin, roxithromycin, tiamulin and tylosin were...

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Báo cáo khoa học: "Determination of Roxithromycin by Liquid Chromatography/ Mass Spectrometry after Multiple-Dose Oral Administration in Broilers" pdf

Ngày tải lên : 07/08/2014, 17:22

... was carried out by the liquid- liquid extraction with borate buffer (pH 9.0) and dichloromethane In short, each g muscle sample was added to ㎖ of borate buffer and homogenized, and then shaken ... roxithromycin was estimated by the linear regression analysis of the log-transformed tissue concentrations, and was determined at the time when the Determination of Roxithromycin by Liquid Chromatography/ Mass ... erythromycin and related substances by high-performance liquid chromatography J Chromatogr 1987, 409, 91-100 Croteau, D., Vallee, F., Bergeron, M and Lebel, M High-performance liquid chromatographic...

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Identification of metabolites of meisoindigo from interspecies microsomes and in vivo biological fluids in rats by liquid chromatography tandem mass spectrometry

Ngày tải lên : 14/09/2015, 14:08

... factors and external factors (Gibson and Skett, 1994) Internal factors involve species, genetics, age, sex, hormones (e.g pituitary gland, sex glands, adrenal glands, thyroid gland, pancreas and ... urine, feces collection and sample preparation……111 5.2.3 Chromatography and mass spectrometry conditions for rat plasma and feces………………………………………………………113 5.2.4 Chromatography and mass spectrometry ... (GC-MS), liquid chromatography (LC) coupled with ultraviolet (UV), fluorescence, radioactivity or tandem mass spectrometry (MS/MS) Among these technologies, liquid chromatography coupled with tandem...

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Tài liệu Báo cáo khoa học: The resident endoplasmic reticulum protein, BAP31, associates with c-actin and myosin B heavy chain Analysis by capillary liquid chromatography microelectrospray tandem MS ppt

Ngày tải lên : 20/02/2014, 23:20

... caspases, as assayed by the processing of procaspases and 3, by the measurement of total DEVDase activity, and by the cleavage of the caspase targets poly(ADP-ribosyl) polymerase and endogenous BAP31 ... BAP31–Flag and the endogenous BAP31 proteins, migrating at apparent masses of 33 kDa and 28 kDa, respectively, and two additional protein bands at apparent masses of 42 and 190 kDa [9] For sequence analysis, ... correlated to the database by SEQUEST, indicating the potential presence of post-translational modiﬁcations Peptide mapping by capillary liquid chromatography microelectrospray tandem MS (LC-lESI-MS/MS)...

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DETERMINATION OF ORGANIC COMPOUNDS IN DRINKING WATER BY LIQUID-SOLID EXTRACTION AND CAPILLARY COLUMN GAS CHROMATOGRAPHY/MASS SPECTROMETRY pdf

Ngày tải lên : 22/03/2014, 14:20

... rules and land disposal restrictions The laboratory using this method has the respons-ibility to protect the air, water, and land by minimizing and controlling all releases from fume hoods and ... solutions 3.12 Calibration Standard (CAL) A solution prepared from the primary dilution standard solution or stock standard solutions and the internal standards and surrogate analytes The CAL ... carcinogens Pure standard materials and stock standard solutions of these compounds should be handled with suitable protection to skin, eyes, etc EQUIPMENT AND SUPPLIES (All specifications are...

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Analysis of intact bacteria, bacterial DNA and mutagenic alkaloids by capillary electrophoresis

Ngày tải lên : 15/09/2015, 17:11

... principles of capillary electrophoresis 1.4 Different modes of capillary electrophoresis 19 1.5 Instrumentation for capillary electrophoresis 24 1.6 Scope of research 32 Part I 35 Analysis of intact ... mode for a specific sample separation Basically, CE modes include capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), micellar electrokinetic chromatography (MEKC), capillary ... species by capillary electrophoresis with blue LED induced fluorescence 61 2.3.3.1 Effect of pH on EOF and migration behavior of bacteria 61 2.3.3.2 Quantitative analysis 65 2.3.3.3 Fish fluids analysis...

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Báo cáo Y học: Functional studies of the Synechocystis phycobilisomes organization by high performance liquid chromatography on line with a mass spectrometer docx

Ngày tải lên : 08/03/2014, 16:20

... gradient bands, HPLC analysis revealed that a- and b-phycocyanins Ó FEBS 2002 are the most abundant components Their presence is particularly prevalent in the heavier bands (bands 3–5), and it is ... proteins found by HPLC-ESI-MS correspond to the bands observed by SDS/PAGE with the exception of the band over 45 kDa, which was previously interpreted as particle containing both phycocyanin and allophycocyanin ... hydrophilic proteins such as linkers and a signiﬁcant amount of a- and b-phycocyanins, and only traces of the allophycocyanins On the contrary, HPLC analysis of bands 2–5 revealed that linker proteins...

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Báo cáo "DETERMINATION SULPHONAMIDES RESIDUES IN FOOD BY HIGH PERFOPvMANCE LIQUID CHROMATOGRAPHY PART 2. SEPARATION AND QUANTIFICATION SULFAGUANIDINE, SULFAMETHOXYPYRIDINE, SULFADOXINE AND SULFAMETHOXAZONE IN ANIMAL FOODSTUFFS AND FOOD PRODUCTS " pdf

Ngày tải lên : 20/03/2014, 21:20

... sulfonamide in meat by solid-phase extraction and capillary electrophoresis, Analytica Chimica Acta 499 (2003) 215-221 Leo M N - Nollet Food analysis hy HPLC, Second edition Revired and Expanded, Marcel ... using liquid chromatography coupled to electrospray tandem mass spectrometry Journal of Chromatography A 1157 (2007) 115-121 Guillermina Font Ana Juan-Garcia, Yolanda Pied - Pressurized liquid ... RESIDUES IN FOOD BY HIGH PERFOPvMANCE LIQUID CHROMATOGRAPHY PART SEPARATION AND QUANTIFICATION SULFAGUANIDINE, SULFAMETHOXYPYRIDINE, SULFADOXINE AND SULFAMETHOXAZONE IN ANIMAL FOODSTUFFS AND FOOD PRODUCTS...

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Báo cáo Y học: Characterization of heparin binding by a peptide from amyloid P component using capillary electrophoresis, surface plasmon resonance and isothermal titration calorimetry ppt

Ngày tải lên : 31/03/2014, 23:20

... approximately 85 lA at a capillary temperature thermostatting of 20 °C The capillary was rinsed after electrophoresis for with each of the following: 0.1 M NaOH, water, and electrophoresis buffer ... concentration and known distribution between mAP-1 and AP-1 (1 : 0.9 based on peak areas) Correlation coefﬁcients (r2) of standard curves were > 0.98 Binding assays were performed by analysis of ... (1995) Preparation and structural characterization of large heparin-derived oligosaccharides Glycobiology 5, 83–95 Heegaard, N.H.H (2001) Capillary Electrophoresis In Protein– Ligand Interactions:...

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Chiral Capillary Electrophoresis in Current Pharmaceutical and Biomedical Analysis potx

Ngày tải lên : 28/06/2014, 06:20

... namely capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), isotachophoresis (ITP), isoelectric focusing (IEF), capillary electrokinetic chromatography (EKC) and capillary ... Chiral Capillary Electrophoresis in Current Pharmaceutical and Biomedical Analysis Figure 2.12 Separation principle of capillary electrochromatography Chiral CEC stationary phases included in capillary ... (chiral) analysis of low molecular ionic (and in some cases also neutral) compounds, such as drugs, their metabolites, Chiral Capillary Electrophoresis in Current Pharmaceutical and Biomedical Analysis...

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báo cáo khoa học: " Evaluation of protein pattern changes in roots and leaves of Zea mays plants in response to nitrate availability by two-dimensional gel electrophoresis analysis" pdf

Ngày tải lên : 12/08/2014, 03:21

... were affected by nitrogen availability The analysis of these spots by LC-ESI-MS/MS allowed to identify 15 and 14 proteins in root and leaf patterns, respectively These proteins and the changes ... sugars and sucrose in roots (A, C, E and G) and leaves (B, D, F, and H) and chlorophyll content in leaves (I) of Zea mays plants, previously grown in the absence of nitrogen for 17 days (T0) and ... Scheible and co-workers [7] reports a comparative transcriptomic analysis of Arabidopsis thaliana seedlings grown in sterile liquid culture under nitrogen-limiting and nitrogen-replete conditions by...

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Báo cáo y học: "Quasi-MSn identification of flavanone 7-glycoside isomers in Da Chengqi Tang by high performance liquid chromatography-tandem mass spectrometry." ppsx

Ngày tải lên : 13/08/2014, 15:21

... herbs and material using microscopic identification method Instruments and conditions High-performance liquid chromatography- diode array detection (HPLC-DAD) analysis was performed on a liquid chromatography ... China) before HPLC analysis Results and discussion Optimization of sample pre-treatments procedure and separation conditions Various methods including liquid- liquid extraction and solid-phase extraction ... i.e methanol and water with 0.2% formic acid (60:40), and the column temperature (45°C) improved the separation of naringin, hesperetin and neohesperetin and helped obtain symmetrical and sharp...

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Báo cáo y học: "Comparison of raw and processed Radix Polygoni Multiflori (Heshouwu) by high performance liquid chromatography and mass spectrometry" pptx

Ngày tải lên : 13/08/2014, 15:21

... (H)-pyran-4-one and 5-hydroxymethyl furfural; P-RPM contains less amino acids and monosaccharides and has a lower pH value than R-RPM [31] In recent years, high performance liquid chromatography (HPLC) and ... raw and processed Radix Polygoni Multiflori (Heshouwu) by high performance liquid chromatography and mass spectrometry Chinese Medicine 2010 5:29 Submit your next manuscript to BioMed Central and ... (Labscan, Thailand) was used for preparation of standards and sample extraction Reference compounds of 2,3,5,4′-tetrahydroxystilbene-2-O-b-D- glucopyranoside (THSG, 1), emodin (2) and physcion...

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Simultaneous determination of sildenafil, vardenafil and tadalafil as forbidden components in natural dietary supplements for male sexual potency by high performance liquid chromatography–electrospray ionization

Ngày tải lên : 02/09/2015, 13:06

... contained in samples Sample Plant sources Oral liquid preparation Oral liquid preparation Oral liquid preparation Oral liquid preparation Oral liquid preparation Wine Wine Wine Beverage Barbary ... ﬂuid products such as wine, beverage, and oral liquid formulation, etc Hence, eight liquid products for examination (ﬁve oral liquid formulation, two wines, and one beverage, their herbal constituents ... sodiated ions The voltage of capillary, extractor and RF lens was set at 3.2 kV, and 0.5 V, respectively The temperature was maintained at 105 and 200 ◦ C for source and desolvation, respectively...

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Applications of capillary electrophoresis in the analysis of natural products

Ngày tải lên : 11/09/2015, 14:27

... Introduction 1.1 Principles and historical perspective of Capillary Electrophoresis Since the introduction of capillary electrophoresis (CE) in 1980s by Jorgenson, the good performance and relatively simple ... conventional slab gel electrophoresis, and produces longer analysis times Performing electrophoresis in a capillary allows the heat to be effectively dissipated through the capillary walls and subsequently ... coating capillary wall (hydrophillicity or charge) Stability often problematic Table 1.2 Different Modes of High Performance Capillary Electrophoresis CE Mode Capillary zone electrophoresis (CZE) Capillary...

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