giải pháp phát triển quan hệ thuơng mại và đầu tư giữa việt nam và nhật bản trong thời kỳ thực thi vjepa
Báo cáo khoa học: " Uncoupling GP1 and GP2 expression in the Lassa virus glycoprotein complex: implications for GP1 ectodomain shedding" ppt
... purification, this protein was captured by the resin, as indicated by the lack of trace detection in the unbound fraction (Figure 3A, lane 5) Analysis of the eluate fraction revealed two strong protein ... utilized in the retrieval of TM proteins to the ER and that binding of the SP to this motif masks localization to this organelle, thereby facilitating the mobilization of the glycoprotein complex ... unclear at this juncture whether GPCTMICFLAG and GPCTM-FLAG proteins are specifically but inefficiently cleaved by SKI-1/S1P protease as a result of normal virus protein processing or if this phenomenon...
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Lecture 3 DNA RNA and protein synthesis great
... All living things are made ofWater (an inorganic compound) Other inorganic compounds (mostly salts) Organic ... Lipids(fats) Proteins* Nucleic Acids* *These are what we’ll talk about today When we look at a living thing, What we see is mostly PROTEIN So, how does an organism produce its particular protein? ... A polymer {a compound made of repeating subunits} WHAT DOES “DNA” STAND FOR? DNA’s proper name isDeoxyribonucleic acid! Consists of a ribose SUGAR with a “missing oxygen” (that’s the de-oxy...
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Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
... was unable to use this RNA as template for RNA synthesis It could be argue that this data results from a misfolding of this short RNA due to a bimolecular association However, this last hypothesis ... respectively The strong effect of the deletion of nt 219–239 on RNA binding correlated with the effect of this deletion on RNA synthesis, suggesting an important interaction between this domain and ... deleted Results with this construction displayed a slightly but significantly reduced RNA synthesis in the presence of heparin compared to that observed in the absence of this compound Our further...
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Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot
... mature peptide was the only one that had been isolated [3] In this work, we have applied the techniques of molecular biology to this important species of industrial exploitation We have isolated, ... * indicates the conserved amino acids within penaeid species ‘:’ indicates similar amino acid The gray shaded boxes indicate the amino acids conserved within the CHH family neuropeptides A B Fig ... muscle or stomach This is in agreement with previous finding that have described the eyestalk X-organ–sinus gland complex as the principal source for the CHH family of neuropeptides [1] This result...
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Báo cáo khoa học: De novo RNA synthesis by a recombinant classical swine fever virus RNA-dependent RNA polymerase pot
... was used as template and newly synthesized RNA products were quantified by real-time quantitative RT–PCR (TaqMan assay), which allowed quantification of the starting copies of the reaction, rather ... visualized by ethidium bromide staining The expected fragments were 228 nucleotides (newly synthesized minus-strand RNA) and 373 nucleotides (synthesized plus-strand RNA) in length host factors This result ... mM dithiothreitol, at room temperature for h After washing with buffer A, membranes were transferred to RNA binding buffer (10 mM Tris/HCl, 100 mM NaCl, 5% glycerol, mM MgCl2, 0.2 mM dithiothreitol,...
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cdna preparation and characterization
... units of XhoI Repeat this procedure for the cut-check sample and incubate it for hr at 37° To reduce the background of the vector, a third cleavage can be performed at a third site on the A DNA ... The total double-strand cDNA quantity is determined simply by multiplying by the quantity of second-strand cDNA Keep a record of the counts per minute obtained at this point to use in a later ... s are localized within dendrites and raised the notion that dendritic mRNA-targeting mechanisms in the dendrite existed Translation of a reporter-tagged m R N A construct within isolated dendrites...
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Báo cáo sinh học: " Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein" pot
... Glutathione S tranferase (GST) pull-down assay pGex-NP and pGex-VP35 were transformed into the BL21 strain of E coli, and expression of the respective proteins was induced by isopropyl-ß-D-thiogalactopyranoside ... control) Complexes were pulled down with glutathion-sepharose, separated on SDS-PAGE and analyzed using a BioImager Binding of NP to GST-NP was set to 100% (E) Quantification of separate experiments ... VP35 and GST The resultant complexes were precipitated with glutathione-sepharose and the amounts of precipitated NP mutants were quantified by BioImaging (Figs 4C and 4D) NP mutants containing...
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Báo cáo sinh học: " Stimulation of poliovirus RNA synthesis and virus maturation in a HeLa cell-free in vitro translation-RNA replication system by viral protein 3CDpro" docx
... RNA-replication reactions This mutant protein exhibited a 2-fold stimulation in RNA synthesis, only slightly lower than what is obtained with 3CDpro(3CproH40A) (Fig 2A) This result indicates that ... possibility that this protein has an additional role at a subsequent step in the viral life cycle, the encapsidation of the progeny viral RNAs To examine at which step of assembly this might occur, ... label that appear in this 80S peak following SDS treatment is not derived from the 155S peak, presumably by the dissociation of provirions into 80S empty capsids and RNA (Fig 6A) This suggested to...
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Báo cáo hóa học: " Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein" pdf
... Glutathione S tranferase (GST) pull-down assay pGex-NP and pGex-VP35 were transformed into the BL21 strain of E coli, and expression of the respective proteins was induced by isopropyl-ß-D-thiogalactopyranoside ... control) Complexes were pulled down with glutathion-sepharose, separated on SDS-PAGE and analyzed using a BioImager Binding of NP to GST-NP was set to 100% (E) Quantification of separate experiments ... VP35 and GST The resultant complexes were precipitated with glutathione-sepharose and the amounts of precipitated NP mutants were quantified by BioImaging (Figs 4C and 4D) NP mutants containing...
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Báo cáo hóa học: "Preparation and characterization of carbon nanofluid by a plasma arc nanoparticles synthesis system" pot
... The thickness of carbon nanoparticles is much smaller than its length and width in the photographs Overall, the shape of these nanoparticles is of the shape of flakes (d-Spacing about 0.35 nm) This ... nanofluid, and cannot cover all factors Since this study is made of non-spherical carbon nanoparticles, Maxwell’s equation will show an undervalue Moreover, this study found that low concentrations ... conductivity of carbon/water nanofluid Furthermore, the carbon nanoparticles made in this study are flake shaped, in which the thickness of the nanoparticle is much smaller than the length and width respectively,...
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Báo cáo hóa học: " Intermatrix synthesis: easy technique permitting preparation of polymer-stabilized nanoparticles with desired composition and structure" ppt
... sensitivity in this case is far lower than that of sensors modified with Cu0NPs after identical treatment Conclusions The main conclusion, which can be derived from the results of this study, concerns ... on IMS of low-solubilitymetal-salt-NPs (i.e metal sulphide NPs) and nanocomposites on their base This communication reports the use of IMS of CuS and PbS INPs along with characterization of the ... bi-metallic core-shell, tri-metallic core-sandwich, etc) The results presented in Figure confirm this hypothesis showing TEM images and EDS spectra of bi-metallic core-shell Pt@Cu (Figure 1a, b)...
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Báo cáo khoa học: "Vegetative propagation of oak (Quercus robur and Q petraea) by cutting and tissue culture" pot
... grow within 1-2 weeks Among the media tested, the highest multiplication rate was obtained on low salt media (BTM, WPM) supplemented with a low concentration of cytokinin (BAP 0.2-0.6 mg·l Within ... unsuitable for rapid clonal propagation The use of cuttings from young plants is limited because the quantity of cutting material which is produced by young ortet is low The rooting ability of older ... in our experiments with vegetative propagation of Q robur and Q petraea by cuttings and by tisIn this sue culture are presented and discussed MATERIALS AND METHODS Propagation by cuttings Leafy...
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Báo cáo sinh học: "Therapeutic dendritic cell vaccine preparation using tumor RNA transfection: A promising approach for the treatment of prostate cancer" pps
... Roughly one-third of these patients progress to incurable metastatic disease, for which there are few treatment options The chemotherapy currently used has limited efficacy [3,4] This lack of ... HLA-A2-restricted peptides [13,14] For other populations, this approach might not be an option because of the high genetic diversity observed This is the case for the Brazilian population, which consists ... mismatch among patients and leukapheresis donors This difference can provoke immune responses against donor DCs instead of the tumor proteins that they carry This problem could be solved by using patient...
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Cambridge.University.Press.The.Crisis.of.Literature.in.the.1790s.Print.Culture.and.the.Public.Sphere.Nov.1999.pdf
... have influenced this book in less direct but more fundamental ways: Steve Boyd, Janice Fiamengo, Scott McFarlane and Tarik Kafala have all insisted on the larger contexts within which this sort of ... in this period It is not only by understanding more about each of them, but also by trying to think through their points of interconnection, that we can better recognize our own implication within ... plans for a panopticon – which reversed this dynamic by emphasizing the visibility of the subjects rather than the rulers Whereas Foucault’s sense of this historical shift is pessimistic (modern...
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Cambridge.University.Press.War.Land.on.the.Eastern.Front.Culture.National.Identity.and.German.Occupation.in.World.War.I.May.2000.pdf
... place Since this study deals above all with German perceptions of the occupied East, which military authorities claimed as a ‘‘New Land’’ for German administration, this study uses German names given ... underlined this ethnic complexity, especially the amazing case of the ‘‘three Smiths’’ in the Lithuanian town of Mariampol Besides a family by the German name of Schmidt, there were also in this multilingual ... historical circumstance and (to them this seemed most obscene) on personal choice and commitment Whatever feelings came to them at the sight of this variety, one thing was clear to Germans They could...
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IELTS Writing Preparation
... culture It promotes the stronger cultures of countries such as Britain and North America and weakens the cultures of less wealthy countries This is because the stronger, wealthier countries are able ... Describing the difference between two levels This year unemployment has increased by 20,000 cases (the difference between this year and last year is 20,000 cases) This year there has been an increase ... ended their schooling at this point This is in stark contrast with 1945 when only 30% of girls completed primary school, 35% had no schooling at all and 35% only completed the third grade In conclusion,...
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Preparation And Practice
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose