giá trị cảnh quan dải ven sông hồng và các hồ nước lớn ở nội thành hà nội
Mechanisms in dendrite pruning of drosophila dendritic arborization neurons
Ngày tải lên :
14/09/2015, 08:38
... supra-pyramidal bundles transforming growth factor-β thoracic ventral neuronsecrectory cells ubiquitin-proteasome system ultraspiracle ventral nerve cord wL3 WP β-gal wandering 3rd instar larva ... muscle tissue layer and contain a single axon and multiple dendrites The axon projects ventrally to the ventral nerve cord (VNC) where it slightly branches out to form axon terminals, while dendrites ... defects at 18 h APF (J) Quantitative analysis of the number of 1° and 2° dendrites attached to the soma 38 of wild type and mutant ddaCs at the WP and 18 h APF stages (K) Quantitative analysis...
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Báo cáo khoa học: b-Secretase cleavage is not required for generation of the intracellular C-terminal domain of the amyloid precursor family of proteins pot
Ngày tải lên :
15/03/2014, 10:20
... of the close migration of APLP2 CTFs, quantitative densitometric analysis of each species was not possible However, the 14.8 and 9.6 kDa bands were quantified separately, and the 13.4 and ... at )80 °C until use Quantitative real-time PCR analysis Total RNA was isolated using TRI Reagent (Ambion, Austin, TX, USA) according to the manufacturer’s instructions, and quantified using a Nanodrop ... the total amount of APLP1s, as its deletion caused a 50% decrease in APLP1s level (Fig 2A) Given that overexpression of BACE1 did not lead to a significant increase in the levels of APLP1s (Fig...
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Báo cáo hóa học: " Human cytomegalovirus UL27 is not required for viral replication in human tissue implanted in SCID mice" ppt
Ngày tải lên :
20/06/2014, 01:20
... The wt virus rescued from the BAC, AD169RV, also replicated well in this model and significant quantities of virus were detected at all time points Thus, animals infected with both variants of ... References 10 11 wild type wt 12 multiplicity of infection MOI 13 Sia IG, Patel R: New strategies for prevention and therapy of cytomegalovirus infection and disease in solid-organ transplant recipients ... 13(1):83-121, table of contents McGavin JK, Goa KL: Ganciclovir: an update of its use in the prevention of cytomegalovirus infection and disease in transplant recipients Drugs 2001, 61(8):1153-1183...
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Báo cáo hóa học: " Membrane-associated heparan sulfate is not required for rAAV-2 infection of human respiratory epithelia" pot
Ngày tải lên :
20/06/2014, 01:20
... epitope) Membrane HSPG was then quantified by flow cytometry This analysis demonstrated a dramatic decrease in HSPG membrane presence following treatment with even the lowest amount of heparinase ... number of research groups that have noted a lack of correlation for certain cell types between the quantity of HSPG present at the cell surface and susceptibility to AAV2 infection [15] Kern and ... HSPG may not be required for cell lines to be susceptible to infection and transduction by rAAV-2 Even after cell-membrane surface HSPG removal was confirmed by flow cytometry and immunohistochemistry,...
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Báo cáo y học: "Wildtype epidermal growth factor receptor (Egfr) is not required for daily locomotor or masking behavior in mice" pps
Ngày tải lên :
10/08/2014, 09:20
... three-hour light pulses given during the dark cycle Locomotor Locomotor activity in Egfrwa2/wa2 mice during 12 h:12 h light:dark cycles and three-hour light pulses given during the dark cycle ... with Egfrwa2 in the initial starting population, was verified by PCR-based genotyping Mice were given at least one week to acclimate to their new environment http://www.jcircadianrhythms.com/content/4/1/15 ... defects may have fortuitously contained a high frequency of these or other genetic modifiers given the small number of individuals tested Previous studies utilizing direct infusion of ligand...
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Báo cáo Y học: The cytoplasmic C-terminus of the sulfonylurea receptor is important for KATP channel function but is not key for complex assembly or trafficking pdf
Ngày tải lên :
31/03/2014, 08:20
... deconvolution module) The quantitative assay was performed on the deconvolved images using the OPENLAB advanced measurements module The theory behind the quantitative assay is given in Fig 6A In colocalization ... both fluorescein and rhodamine fluorophores (XF66-1 multiband filter, Omega optical) Images used for quantitative analysis and colocalization experiments represented a focal plane taken through the ... C-terminus of SUR1 does not substantially affect the response to this nucleotide (Fig 3Ci and ii) and quantitation is shown in Fig 5B However other electrophysiological and pharmacological parameters...
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Báo cáo y học: "JNK1 is not essential for TNF-mediated joint disease" doc
Ngày tải lên :
09/08/2014, 06:22
... using a 700-W microwave oven Tissue sections were cooled to room temperature and then rinsed using detergent solution: 0.5% Tween in phosphate-buffered saline (PBS) For quantification of inflammation, ... from JNK1-/hTNFtg mice Quantitative analysis of the area of synovial inflammation revealed no significant differences between hTNFtg and JNK1-/-hTNFtg mice (Fig 3a) Similarly, quantification of erosive ... Positive cells are stained in brown (arrows; magnification 400×) Results of quantification (percentages of positive cells) are given in separate bar charts Vertical bars indicate standard deviation...
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Báo cáo y học: "Human cyclin T1 expression ameliorates a T-cell-specific transcriptional limitation for HIV in transgenic rats, but is not sufficient for a spreading infection of prototypic R5 " ppt
Ngày tải lên :
13/08/2014, 05:21
... hCycT1-tg hCycT1 Figure transgenensis enhances the Tat-responsiveness of rat T-cells hCycT1 transgenensis enhances the Tat-responsiveness of rat T-cells Activated, spleen-derived T-cells from three ... addition, hCycT1-dependence and species-specific differences of HIV LTR driven gene expression appear to be less pronounced or even absent when levels of gene expression per cell are low This was observed ... MDM [4,12,42] The general ability of macrophages to support HIV LTR-driven transcription and, related to that, their responsiveness to transgenic hCycT1 expression, appears to be remarkably different...
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Báo cáo y học: " The carbohydrate at asparagine 386 on HIV-1 gp120 is not essential for protein folding and function but is involved in immune evasion" potx
Ngày tải lên :
13/08/2014, 06:20
... line for the detection and quantitation of a single cycle of HIV and SIV replication Virol 2000, 273:307-315 Baribaud F, Pohlmann S, Leslie G, Mortari F, Doms RW: Quantitative expression and ... glycosylation are shown B Local reversions after evolution A detailed description of the evolution is given in materials and methods section Sequences of the V4 loop and flanking regions of wt, mutant ... standardized for CA-p24 input and the gp120 contents of mutants in the respective fractions are given as percentages of the wt gp120 contents (arbitrarily set at 100) The results are representative...
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Báo cáo y học: "Vpu serine 52 dependent counteraction of tetherin is required for HIV-1 replication in macrophages, but not in ex vivo human lymphoid tissue" docx
Ngày tải lên :
12/08/2014, 23:22
... independent experiments Cell surface CD4 is given as a percentage of that measured on cells transfected with the control vector expressing GFP only (100%) (D) Quantitative analysis of tetherin degradation ... different amounts of tetherin as well as P4-CCR5, PBMC, PBL and MDMs and quantified total protein content using the 2D-quant kit (Amersham Biosciences) 40 μg protein of each lysate were separated ... Additional file 1: Supplementary Figure S1 Assessment of viral release by quantitative WB correlates with p24 ELISA Correlation of the quantitative WB data shown in Fig with ELISA results, that were measured...
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Báo cáo y học: " Incorporation of podoplanin into HIV released from HEK-293T cells, but not PBMC, is required for efficient binding to the attachment factor CLEC-2" ppsx
Ngày tải lên :
12/08/2014, 23:23
... contains 17 amino acid residues highly conserved between C-type lectins [30] Binding of the snake venom toxin rhodocytin to CLEC-2 triggers Syk-dependent signalling in platelets which causes platelet ... minutes at 4°C before staining was analyzed by flow cytometry For all measurements 20,000 gated events were collected Knock-down of podoplanin expression by shRNA For stable knock-down of podoplanin ... within 30 minutes after completion of fixation by flow cytometry For all measurements 20,000 gated events were collected Inhibition of antibody binding by soluble podoplanin The podoplanin specific...
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Tài liệu Báo cáo khoa học: Nop53p, an essential nucleolar protein that interacts with Nop17p and Nip7p, is required for pre-rRNA processing in Saccharomyces cerevisiae pdf
Ngày tải lên :
20/02/2014, 01:20
... [32P]5.8S, 20 pmol bovine serum albumin and 40 pmol of cold nonspecific competitor Lower panel shows quantitation of the protected [32P]5.8S rRNA bands reported in the global yeast protein localization ... b-galactosidase (b-Gal) activity [46] b-Gal activity of strains analyzed in two-hybrid experiments was quantitated 4459 RNA processing in S cerevisiae D C Granato et al Table List of yeast strains used ... recipient of a CNPq fellowship This work was supported by FAPESP grant (03 ⁄ 06031-3 to CCO) References Venema J & Tollervey D (1995) Processing of pre-ribosomal RNA in Saccharomyces cerevisiae Yeast...
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Tài liệu Báo cáo Y học: Phosphorylation of initiation factor-2a is required for activation of internal translation initiation during cell differentiation ppt
Ngày tải lên :
22/02/2014, 07:20
... differentiation process is a cascade of events leading to major changes in gene expression Reduced global protein synthesis is a consequence of upstream events, as inhibition of protein synthesis ... IRES use The IRES elements of human VEGF, human c-Myc and EMCV were cloned into a CMV promoter-driven bicistronic vector, between the coding regions of Renilla and firefly luciferases, as illustrated ... immunoprecipitate by SDS/10% PAGE, the proteins were blotted onto a nitrocellulose membrane and quantified by phosphoimager The membrane was then used for Western analysis using antibodies specific...
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Báo cáo khoa học: YidC is required for the assembly of the MscL homopentameric pore potx
Ngày tải lên :
07/03/2014, 02:20
... periplasm (Fig 2A) Upon lysis of the cells expressing MscL F54C, AMS labelling appeared to be even more efficient, suggesting that a very small proportion of MscL F54C is either not inserted or ... molecular mechanism of MscL folding, oligomerization and quality control has remained unexplored Given recent evidence that, for certain IMPs, YidC is not only required for membrane insertion of ... had no effect on the insertion of MscL, when using the F54C mutant In addition, in our hands, the quantity of MscL present in the inner membrane appeared to be unaltered upon YidC depletion (Fig...
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Báo cáo khoa học: Lpx1p is a peroxisomal lipase required for normal peroxisome morphology potx
Ngày tải lên :
07/03/2014, 05:20
... monomeric, dimeric and trimeric forms of Lpx1p The relative distribution of the three forms was quantified using NIH Image (National Institutes of Health, Bethesda, MD, USA) The elution volume ... HMM– HMM comparison Bioinformatics 21, 951–960 Lametschwandtner G, Brocard C, Fransen M, Van Veldhoven P, Berger J & Hartig A (1998) The difference in recognition of terminal tripeptides as peroxisomal ... that react with the leucine zipper of Jun Proc Natl Acad Sci USA 89, 5789–5793 Stein K, Schell-Steven A, Erdmann R & Rottensteiner H (2002) Interactions of Pex7p and Pex18p ⁄ Pex21p with the peroxisomal...
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Báo cáo khoa học: Caspase-8- and JNK-dependent AP-1 activation is required for Fas ligand-induced IL-8 production ppt
Ngày tải lên :
07/03/2014, 09:20
... the FasL-induced IL-8 production in the untransformed human embryonic kidney cell line, HEK293 Conveniently, using this cell line we can exclude the side-effect of cell death, because this cell ... In this study, we sought to elucidate these points and found that AP-1 activation is a crucial event for FasL-induced IL-8 production, and that the FasL-induced AP-1 activation depends on JNK ... (Fig 4B and [43]) Strikingly, the 293-K cells did not show AP-1 activation upon FasL stimulation even when exogenous human Fas was introduced by transient transfection (Fig 4C) When the wildtype...
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Báo cáo khoa học: Catalytically active membrane-distal phosphatase domain of receptor protein-tyrosine phosphatase a is required for Src activation doc
Ngày tải lên :
15/03/2014, 10:20
... anti-pY416 and anti-Src Igs, as indicated Src phosphorylation was quantified and the values were normalized for the total amounts of Src The quantification results are presented under the corresponding ... with anti-pY789 to assess Tyr789 phosphorylation The amount of co-immunoprecipitated RPTPa was quantified and normalized for total pTyr789 levels The samples with no transfected RPTPa were not ... considerable catalytic activity [10,14,29] It was shown that its activity is comparable to, or even higher than, the activity towards para-nitrophenylphosphate (pNPP) of dual-specificity phosphatases...
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Báo cáo khoa học: Knock-out of the chloroplast-encoded PSI-J subunit of photosystem I in Nicotiana tabacum PSI-J is required for efficient electron transfer and stable accumulation of photosystem I pot
Ngày tải lên :
16/03/2014, 11:20
... by side, and, for each antibody, the resulting signal was quantified using the LabWorks software as described in Experimental procedures Quantification was performed on two independent preparations ... than the wild-type plant extraction of leaf discs using boiling ethanol and spectrophotometric quantification showed a 13% reduction in the content of Chl per leaf area compared with wild-type ... Fig Immunoblot analysis of proteins in PSI particles prepared from DpsaJ and wildtype plants (A) Quantification of the signals obtained in the immunoblot analysis (B) Representative example of the...
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Báo cáo khoa học: Multi-tasking of nonstructural gene products is required for bean yellow dwarf geminivirus transcriptional regulation potx
Ngày tải lên :
16/03/2014, 13:20
... synthesis from the 5¢-terminus As DNA synthesis progresses, the virion sense-strand is displaced and eventually is recircularized and religated by Rep [1,4–6] The LIR contains sequences responsible ... vivo conditions Finally, regulation of BeYDV CP expression was examined The discovery of an unconventional mechanism of translational initiation is discussed Results Comparison of Pc and Pv promoter ... along with various constructs expressing BeYDV gene products, and complementary gene expression was quantified by assay for GUS activity (Fig 3A) Construct p35SDBRep, containing a large deletion within...
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