future targets for engineering new lignins
plant biotechnology and transgenic plants - kirsi-marja oksman-caldentey , wolfgang h. barz
... preserved for long periods of time Such "pools of genes" represent the basis for sustainable development and allow future programs for improved adaptation of plants to human needs Fortunately, ... University, New Brunswick, New Jersey Mohammad Pessarakli, University of Arizona, Tucson Donald R Nielsen, University of California, Davis Jan Dirk van Elsas, Research Institute for Plant Protection, ... Another interesting aspect of the search for new secondary products is the fact that most likely all plants have a much greater genetic potential for the formation and accumulation of such products...
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Báo cáo y học: " Low NO bioavailability in CCl4 cirrhotic rat livers might result from low NO synthesis combined with decreased superoxide dismutase activity allowing superoxide-mediated NO breakdown: A comparison of two portal hypertensive rat models wit
... (Transduction Labs, Lexington, USA) for eNOS; a homogenate of rat brains for nNOS; and a liver of a rat given LPS 800 µg/kg IV (Sigma, St Louis, USA) hours before harvesting for iNOS Samples of both portal ... the Bradford method (Bio-Rad Labs, Page of (page number not for citation purposes) Comparative Hepatology 2003, http://www.comparative-hepatology.com/content/2/1/2 Table 1: Livers used for NOS ... stopped by heating in boiling water for The PCR primer set used for the detection of rat caveolin1 mRNA (access number Z 46614) was: Page of (page number not for citation purposes) Comparative...
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Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx
... QC774 transformants were screened on 40 M63 minimal media agar plates containing lm paraquat for each randomly point-mutagenized library In addition, a control transformation was performed together ... expressing Q143A hMnSOD was used to transform QC774 cells, and this transformant was plated onto a lm paraquat plate so as to obtain approximately 4000 transformants The growth (or lack of growth) ... formation of an inactivated species of MnSOD [19,20] This zero-order region predominates in the progress curves for strongly inhibited variants of MnSOD Values of the rate constant k0 ⁄ [E] for...
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Báo cáo Y học: ER–resident chaperone interactions with recombinant antibodies in transgenic plants potx
... denatured by boiling for in vol formamide before use Northern blotting Fifteen lg of total plant leaf RNA were prepared in 10· Mops buffer, 5% (v/v) formaldehyde, and 50% (v/v) formamide Samples ... °C for 10 min, and Ó FEBS 2002 6044 J Nuttall et al (Eur J Biochem 269) run on a 1.2% (w/v) agarose gel prepared with 2.2 M formaldehyde The gel was stained in ethidium bromide, then soaked for ... chain transgenic tobacco 6046 J Nuttall et al (Eur J Biochem 269) from nontransformed and transformed plants and looked for coimmunoprecipitation of BiP with the recombinant proteins (Fig 4) Initially,...
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Báo cáo hóa học: " A predominance of R5-like HIV genotypes in vaginal secretions is associated with elevated plasma HIV-1 RNA levels and the absence of anti-retroviral therapy" docx
... molecules utilized for entry [7] The nucleotide sequence of the HIV envelope V3 region determines whether the virion utilizes a CCR5 (R5) or CXCR4 (X4) coreceptor molecule, therefore the V3 genotype ... secretions, and assessed ART use in a cohort of 43 women attending an HIV outpatient clinic in New Orleans Informed consent was obtained in accordance with Louisiana State University Health Sciences ... 105 copies/vaginal sample For statistical analysis, HIV RNA levels in both plasma and vaginal secretions were categorically designated as high-level HIV or low-level HIV for each, as defined in...
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Báo cáo y học: "A simple hepatic cyst with elevated serum and cyst fluid CA19-9 levels: a case report" pps
... A) Enhanced abdominal computed tomography before a percutaneous transhepatic drainage of the hepatic cyst A) Enhanced abdominal computed tomography before a percutaneous transhepatic drainage of ... in our patient with a simple hepatic cyst before treatment, challenging this suggestion Elevated serum and cyst fluid CA19-9 levels in our patient before treatment may be due to an infected hepatic ... advised on the format and design and assisted in providing a critical appraisal of the manuscript Both authors have reviewed and approved the final manuscript 40 20 Consent Written informed consent...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... University) for performing ultracentrifugation analysis and Mr K Mieda and M Sakata (Tottori University) for technical assistance in enzyme assay This study was supported by a Grant-in-Aid for Scientific ... ApeSOD was approximately 20-fold more active in its Mn-bound form than in its Fe-bound form (Table 1) We propose two possible explanations for this finding One is related to inhibition of the binding ... structure of SOD from A pernix K1 activity in the Mn- and Fe-forms, and the other exhibiting low activity in the Fe-form and high activity in Mn-form ApeSOD belongs to the latter group [16] This was...
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Tài liệu Design of Feedback Control Systems for Stable Plants with Saturating Actuators ppt
... initial investigation was made on the effects on performance of the reset windups for MIMO systems [11] showing potential for improving the performance of the system A simple case study was also ... closed loop system There are well developed methods for defining performance criteria and for designing linear closed loop systems which meet the performance requirements It would then be desirable, ... Thomson, " New desaturation strategy for digital PID controlers", IEE Proceedings, Vol 130, Pt D, No 4, July 1983, pp.1 13 [7] P Gutman and P Hagander, " A New Design of Constrained Controllers for...
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Tài liệu Báo cáo khoa học: The solution structure of reduced dimeric copper zinc superoxide dismutase doc
... conformation For all the ligands, the rmsd value ˚ calculated for all heavy atoms is smaller than 0.8 A, a value that is similar to that obtained for secondary structure elements The ligand conformation ... side-chain orientation is optimized for correctly orienting the incoming superoxide anion for the electron transfer process The equilibrium between multiple conformations for this group and a different ... with respect to the dimeric one In particular, conformational equilibria were observed for residues around Cys57 and Cys146 The former residue forms an H-bond with the guanidinium group of Arg143...
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Báo cáo khoa học: A superoxide dismutase–human hemoglobin fusion protein showing enhanced antioxidative properties pptx
... steric difficulties encountered in forming the necessary SOD tetramers [7,12] The tetrameric conformation is important for the formation of an active site suitable for Mn ligation [12] An alternative ... the degradation products (Fig 6) For all H2O2 concentrations used (1, and mm), the amount of heme degradation for SOD–Hb was much lower than for Hb 6198 Fig The formation of fluorescent heme degradation ... constants were determined to be 0.18 h)1 for Hb and 0.10 h)1 for SOD–Hb at room temperature The rate constant for SOD–Hb is thus approximately half of that for Hb, although higher than the values...
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Báo cáo khoa học: Betulinic acid-mediated inhibitory effect on hepatitis B virus by suppression of manganese superoxide dismutase expression pot
... virus 0.2 mgÆmL)1 proteinase K for h at 42 °C, and then for h at 65 °C, to reverse the formaldehyde crosslinking DNA fragments were recovered by phenol ⁄ chloroform extraction and ethanol precipitation ... (Sigma), and incubated at 37 °C for no less than h, with continuous stirring The temperature was then increased slowly up to boiling point, and maintained for 10 for decoction The solution was ... 25 °C for h Thereafter, the precipitated blue formazan product was extracted by incubating samples with 0.1 mL of 10% SDS (dissolved in 0.01 m HCl) overnight at 37 °C The absorbances of formazan...
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TRANSGENIC PLANTS docx
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25