free nucleotides in living cells play important roles in a variety of biological reactions
Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy in living cells doc
... pcitrine-N1.phPGK1–7aa–cerulean and phPGK1–5aa–cerulean wereconstructed using the primers 5¢-CCGGA ATTCCAATGT CGCTT TCTAA CAAGC T-3¢ and 5¢-GGCGGATCCA TAATA TTGCT GAGAG CATCC A- 3¢, and 5¢-CCGGA ATTCC AATGT ... activities of GAPDH–citrine and PGK–cerulean. (A) Expression of GAPDH–citrine andPGK–cerulean. Localization of endogenous GAPDH and PGK in HeLa cells was examined by using antibodies against GAPDH andPGK, ... being independent of thestrength of protein–protein interactions. In the case of very weak interactions as described in this article,rapid association and dissociation of FRET pairs mayalso...
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Tài liệu Báo cáo khoa học: Mixed lineage leukemia histone methylases play critical roles in estrogen-mediated regulation of HOXC13 docx
... GGAGCAAGAGGTTCAGCATCMLL2 GTGCAGCAGAAGATGGTGAA GCACAATGCTGTCAGGAGAAMLL3 AAGCAAACGGACTCAGAGGA ACAAGCCATAGGAGGTGGTGMLL4 GTCTATGCGCAGTGGAGACA AGTCTGCATCCCCGTATTTGHOXC13-ERE1 GCGTCTCCCTGTCCCTTTA CAGGTCTCCTGGGGTTCCHOXC13-ERE2 ... TTGCCGAGTATATTCCATTGC TCTGCTTTACCTCGCTGGATHOXC13-ERE3 TTTCAGGCCCTTTGTTTCTC CGCGGGTAGTAGAAGTGGAAHOXC13-ERE4 TGCCCTCATATAAACCTGGAA AGCCTTTGGGAGTAGGAACCERa antisense CATGGTCATGGTCAG a ERb antisense ... Forward primer (5¢-to3¢) Reverse primer (5¢-to3¢)b-Actin CTCTTCCAGCCTTCCTTCCT AGCACTGTGTTGGCGTACAGHOXC13-ORF GGAAGTCTCCCTTCCCAGAC CGATTTGCTGACCACCTTCTMLL1 GAGGACCCCGGATTAAACAT GGAGCAAGAGGTTCAGCATCMLL2...
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Báo cáo khoa học: Ternary complex formation of pVHL, elongin B and elongin C visualized in living cells by a fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy technique docx
... 5¢-CTAGAGTTAACCCGGGATATCCAGGTCTTCCTC ACTGATCAGCTTCTGTTCCTCCATGGTGGT-3¢, and 5¢-CTAGACCACCATGGACTACAAAGACGATGACGATAAAGATATCCCGGGTTAACT-3¢ and 5¢-CTAGAGTTAACCCGGGATATCTTTATCGTC ATCGTCTTT GTAGTCC ... annealedfragment of the synthesized oligonucleotides, 5¢-CTAGACCACCATGTACCCCTACGACGTGCCCGACTACGCCGATATCCCGGGTTAACT-3¢ and 5¢-CTAGAGTTAACCCGGGATATCGGCGTAGTCGGGCACGTCGTAGGGGTACATGGTGGT-3¢, into ... XbaI site of pBOS Vector.pBOS-Myc and pBOSFlag were constructed similarly byusing the synthesized oligonucleotides 5¢-CTAGACCACCATGGAGGAACAGAAGCTGATCAGTGAGGAAGACCTGGATATCCCGGGTTAACT-3¢ and...
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Báo cáo khoa học: FLIP and MAPK play crucial roles in the MLN51-mediated hyperproliferation of fibroblast-like synoviocytes in the pathogenesis of rheumatoid arthritis pdf
... using appropriateprimer pairs for cDNAs of hMLN51: full-length, 5¢-TATGAATTCGTTCTCCGTAAGATGGCGGAC-3¢ and 5¢-TATCTCGAGTTAACTGGAACCCCTGCTTACAA-3¢; par-tial length, 5¢-ATCAAGCTTTGGTGCGTAAGGAGCTGAC-3¢ ... differential localization, andregulation of the stress-activated protein kinases, extra-cellular signal-regulated kinase, c-JUN N-terminalkinase, and p38 mitogen-activated protein kinase, in synovial ... 5¢-GCCAGCAGCATCTTCACGTC-3¢; NF-kB(p65): sense, 5¢-GACAATCGTGCCCCCAACAC-3¢; anti-sense, 5¢-TGGGTCCGCTGAAAGGACT-3¢;human b-actin: sense, 5¢-TGACGGGGTCACCCACACTGTGCCCATCTA-3¢; anti-sense, 5¢-AGTCATAGTCCGCCTAGAAGCATTTFCGGT-3¢.siRNA...
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Investing in Nursing Education to Advance Global Health: A position of the Global Alliance for Leadership in Nursing Education and Science pptx
... RN-to-baccalaureate programs, a 10.8% increase in master’s programs, a 10.4% increase in research-focused doctoral programs, and a 35.3% increase in Doctor of Nursing Practice programs. Interest in ... Enrollment and graduations in baccalaureate and graduate programs in nursing. Washington, DC: Author. Australian Institute of Health and Welfare, (2010). Nursing and Midwifery Labour Force ... States In March 2011, the American Association of Colleges of Nursing (AACN) released data collected in Fall 2010 showing that enrollment in entry-level baccalaureate nursing programs increased...
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Báo cáo khoa học: "Extracting Semantic Roles from a Model of Eventualities" pot
... recognizes (in an abstract sense) the importance of spatio-temporal "regions" in the process of individuation of events [14]. To express the change of localization (again in an abstract ... line of situation seman- tics), and also in AI [10, see also 4]. Between the total refusal to use those labels (as in GPSG) and the acceptance of individual roles (as in HPSG) there is a wide ... [3J/Max em- barks the crate on the cargo boat can be analyzed in two steps. First there is a transition from an initial state IS where the crate is not on the cargo boat to a final state FS...
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Báo cáo khoa học: A novel factor XI missense mutation (Val371Ile) in the activation loop is responsible for a case of mild type II factor XI deficiency doc
... 49–54.Supplementary materialThe following supplementary material is availableonline:Fig. S1. Comparison of the catalytic properties of recombinant and plasma-derived FXIa.This material is available as part ... Bizzi B & Landolfi R (1993)The linkage between binding of the C-terminal domain of hirudin and amidase activity in human alpha-throm-bin. Biochem J 289, 475–480.45 Baglia FA & Walsh PN ... were added to the solution,and the amount of free paranitroaniline released by FXIawas determined by measuring the change in absorbance at405 nm in a Benchmark II microplate reader (Bio-RadLaboratories,...
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Báo cáo Y học: Concerted regulation of free arachidonic acid and hormone-induced steroid synthesis by acyl-CoA thioesterases and acyl-CoA synthetases in adrenal cells pdf
... of PLA2.Our current data indicate the presence of a new pathwaythat regulates intracellular levels of AA, in which ACS4could act by sequestering free AA by esterification intoAA-CoA. CoA-esterified ... (ARTISt)and an acyl-CoA synthetase as members of an alternativepathway in the regulation of the intracellular levels of AA in steroidogenesis. Purified recombinant ARTISt releases AAfrom arachidonoyl-CoA ... protein kinase; AA, arachi-donic acid; AA-CoA, arachidonoyl-CoA; NDGA, nordihydro-guaiaretic acid; StAR protein, steroidogenic acute regulatoryprotein; PLA2, phospholipase A 2; ATK, arachidonoyl...
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Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"
... (5’→3’) Anticipated length (bp) D-Loop ATTCTAACCTGAATCGGAGG GATGCTTGCATGTGTAATCT 1528 ATPase8 CCCGGACGTCTAAACCAAACC GGGGATCAATAGAGGGGGAAATA 512 ATPase6 AATTACCCCCATACTCCTTACACT GGGTCATGGGCTGGGTTTTACTAT ... GGGTCATGGGCTGGGTTTTACTAT 857 COX-I CCTCGGAGCTGGTAAAAA GGGGGTTCGATTCCTTC 1654 COX-II ACTACCCCGATGCATACACCACA GGGCAATGAATGAAGCGAACAG 1333 COX-III GCCGTACGCCTAACCGCTAACA TCGTAAGGGGTGGTTTTTCTATG 1177 Cyt b CGCACGGACTACAACCACGAC ... results in the reduction of ATP synthesized in mitochondria and affect cell viability. Both the pathogenic factors of Hp (VacA and CagA), especially the VacA, play an important role in the Hp induced...
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Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
... cells/ absorbance of control cells. RT-PCR analysis Total RNA was extracted from JAR cells using RNeasy mini kits (QIAGEN, Mississauga, Canada). Two micrograms of total RNA was reverse transcribed, ... an increase in the expression of another antiapoptotic protein, Bag-1, although the expression of Bcl-2 pro-tein was decreased. Bag-1 is a multifunctional protein containing a domain that ... started to appear in the cells. The medium was then aspirated and replaced with DMSO. The absorbance was read at 540nm in a microtiter plate reader. Quantitation of internucleosomal DNA fragmenta-tion...
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- hiv1 efficiently infects susceptible cells and causes aids in humans although hiv can also enter the cells of old world monkeys
- has attracted increasing attention as a component of amperometriclglutamate sensors used in the food industry and clinical biochemistry the precursor of lgox
- an important player in the dna damage response
- semantic roles in framenet
- the girl in the mirror play
- important places in southeast asia
- important verbs in english with hindi meaning
Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose