free ebooks on human anatomy and physiology

... order conditions, using a substrate concentra- tion far below the K m , as reported elsewhere [13]. Calcula- tions were done usin g ENZFITTER software (Biosoft). Excitation and emission wavelengths ... of 4 M urea and a cocktail of inhibitors. T here was no signi®cant degradation of semenogelins under these condi- tions, which allow the rapid separation by centrifugation of cellular components and ... the glutamine±serine bond and the ®ltered fraction at the serine±tyrosine bond, as shown by HPLC fractionation and N-terminal sequencing. Part of the last ®ltered fraction was puri®ed further...