... less sensitive to the pres-ence ofthe anion. These residues also modulate the conformation of unfol-ded cytochrome c, influencing its spin state and the coordination to the prosthetic group.AbbreviationCT, ... with further formationofthe LS(IHH) A-state; (b) for the slow phase, to formation of the LS native-like (IHM) A-state. Unlike wild-typecytochrome c, once the equilibrium is reached, the K88E ... from the 695 nm band; therefore, the observed rate can be attributed to formationof the Fe(III)–M80 axial bond, providing strong indicationthat the slow phase is coupled to formationof the native...
... analysis ofthe las operonFEBS Journal 272 (2005) 2292–2303 ª 2005 FEBS 2293minute. The relative values of simultaneous modulation of the three las enzymes are calculated as the average of the three ... were related to the A280 of the extract, for the purpose of determining relative activities. The specific activ-ities of PFK and PK and LDH in MG1363 were deter-mined as UÆmg)1 of protein, where ... each ofthe las enzymes on the glycolytic flux,growth rate and product formation. We also studiedstrains with modulated expression ofthe entire lasoperon, and show that the data fit well with the...
... over-expressing the Arg252-variant, thesedimers may contribute to theformationof active oligomersin the p resence ofthe endogenously expressed G ly252-containing subunits. T he exact c omposition ofthe ... been ascribed to the insert between Asp and His ofthe catalytic domain of TPP II. We can now report that the region surroundingArg252 is of importance for theformationofthe oligomericenzyme ... bout one tenth of the specific activity ofthe oligomeric complex [15]. For the recombinant human enzyme the nonassociated form alsoappeared to be dimers ofthe 138 kDa subunit, since theirMrwas...
... outside the landward fortifications of the city; the fate of Canada was decided in a battle in the open; the dying Wolfe defeated the dyingMontcalm, and the town surrendered. The fall ofthe rest of ... Down to the day ofthe Declaration of Independence the advantage wasclearly with the colonists. The hard, stern struggle ofthe war began in August, 1776, with the arrival of the British in the ... 38 The second volume ofthe EPOCHS OF AMERICAN HISTORY aims to follow out the principles laid downfor " ;THE COLONIES," the study of causes rather than of events, the development of the...
... possible role of this chaperone in the stabilization ofthe core struc-ture ofthe bc1complex can be excluded on the basis of the existence ofthe 500 kDa bc1sub-complex alsoin the DBCS1 deletion ... to the 500 kDa bc1sub-complex. Therefore, the pres-ence of both Qcr9p and Bcs1p is required for the inser-tion of ISP into the bc1sub-complex, but the presence of only one of these two ... alsounknown. Furthermore, as in the case ofthe biogene-sis of other multi-subunit complexes ofthe mitochon-drial respiratory chain, the assistance of specificchaperone proteins is also required. The...
... assembly of TNSALP in the endoplasmic reticulum (ER), resulting in the loss of functional TNSALP from the cell surface [10–14]. Dele-tion of T at position 1559 ofthe cDNA [TNS-ALP (1559delT)] ofthe ... [16–18]. Accordingto the recommended nomenclature, the adenine of the initiator ATG codon in the cDNA of TNSALP isdenoted as nucleotide +1 instead ofthe first nucleotide of the cDNA clone originally ... specific enzyme activity of the mutant protein was calculated to be about onethird of that ofthe wild type (Fig. 7C). In contrast to the culture medium ofthe cells expressing the wildtype enzyme,...
... the beginning of his dignity to the See ofthe Apostle Peter. For the newly-elected pontiff acknowledges in hisletter that Peter is the chief ofthe Apostles and the Rock ofthe Faith: that the keys ofthe ... the beginning ofthe fifth century was justified by the experience of Theodorich at the beginning ofthe sixth. And, further, we may take the conduct of these two great men asexpressing on the ... 455 is the first of three hundred years of warfare carried on from the time ofthe Vandal through the time of the Lombard, under the neglect and oppression ofthe Byzantine, until, in the year...
... of a4v over the entire sequence defines the aggregationprofile ofthe polypeptide. The hot-spot threshold(HST) was defined as the average ofthe a3v ofthe 20natural amino acids weighted by their ... assigned to the central residue ofthe window. The smoothed expectedvalues for every position ofthe polypeptide chain pro-vide the final profile, which is directly used for the pre-diction of amyloidogenic ... propensity enter the scoring functionas a physical property term Sphyspropconsisting of the sum ofthe products ofthe amino acid frequency with the normalized property value ofthe respective...
... range of substrates through aset of hydrophobic residues located at the tips of the tentacles [34,35,39,52], it has also been shown that thereare basic residues in the distal regions ofthe tentacles ... support the notion that PFDparticipates in theformationof Ab oligomers within the cytosolic compartment.In an effort to elucidate the mechanism pertainingto the PFD-induced formationof high-molecular-masssoluble ... oligomerInhibit/Slow down oligomerizationAβ protofibrilFig. 8. Schematic model oftheformation of soluble Ab oligomers in the presence of PFD. Formation of amyloid beta oligomers by prefoldin M....
... absolute configuration of the starting material, the ee ofthe compounds in the twolayers will increase.After the above mentioned separation procedure, the ee ofthe materials in the organic layer ... interesting from the view point ofthe molecular recognition of this enzyme. The 3D structural analysis may shed light on the answers of these questions.In the case of recombinant rat LACS1, the substratespecificity ... acid.Because the ee ofthe thioesters could not be deter-mined as they existed, the thioester bond was hydro-lyzed and the ee ofthe resulting free acid was measured. The two kinds of hydrolytic...