... 28MYA (Fig. 7). The C. salei hemocyanins and N. inauratasubunit g separated 222 ± 9 MYA. Assuming that scor-pion hemocyanin AauHc6 is associated with the hemocy-anins ofthe Araneae rather than ... of chelicerate hemocyanins.Materials and methodsAnimalsSix specimens ofthe red-legged golden orb-web spider,Nephila inaurata madagascariensis (Chelicerata; Araneae;Tetragnathidae; Fig. 1), ... spiderg-subunits. A timescale of chelicerate hemocyanin evolution wasinferred assuming that the LpoHc2 and the a- subunits of N. inaurata and E. californicum on the one hand, andTtrHcA and the arachnid...
... acquirerBritish Library Cataloguing in Publication DataData availableLibrary of Congress Cataloging in Publication DataData availableTypeset by SPI Publisher Services, Pondicherry, IndiaPrinted ... breaks new ground by adopting a globalapproach to the insula, rather than looking at houses in isolation, and byseeking to interpret the insula in historical ter ms rather than concentratingon ... list of plates the insula ofthe menander at pompeiiThis series arises from the British Pompeii project, initiated in 1978. Its aim is to carry out a complete survey and record ofthe insula...
... literature review, managementand organisation ofthe study, methodology with assist-ance from GT, AS, DG, data collection, data input, dataanalysis and interpretation with assistance from GT andAS. ... treatmentneed (re-categorised into binary variables) using the Mann-Whitney test. The data were analysed using the SPSS statistical package. The cut-off level for statistical significance was set at 0.05.Results232 ... Organisation WH: International Classification of impairments,disabilities and handicaps. Geneva, World Health Organisation;1980. 12. Streiner D, Norman G: Health Measurement Scales. A practical...
... should have appropriate management information systems (MIS) and data processes that enable it to collect, sort, aggregate, and update data and other information efficiently and reliably within ... that it has readily available data and other information forthe types of stress tests it uses, including key variables that drive performance. In addition, a banking organization should have ... complements a banking organization’s regulatory capital analysis8 by providing a forward-looking assessment of capital adequacy, usually with a forecast horizon ofat least two years (with the recognition...
... 51–92.4 Yasumasu S, Yamada K, Akasaka K, Mitsunaga K,Iuchi I, Shimada H & Yamagami K (199 2a) Isolation of cDNAs for LCE and HCE, two constituent proteases of the hatching enzyme of Oryzias latipes, ... 5Â-AAGTTGTAGGCCTTCTGCGGGTTGATGTTC-3Â;5Â-RACE (for nested PCR),5Â-GAGCATGGTTGAT CTCGTGCTGGATGATGC-3Â;3Â-RACE (for rst PCR), 5Â-GTACGACTACATCAGCATCGAGAACAGAGC-3Â; and 3Â-RACE (for nested PCR),5Â-ATGTTTCTCCTCTCTGGGCAGAACTGGAGG-3Â.Two ... 5Â-CCGGTGTGACAAGTCTCAGAAGTGAC-3Â; and reverse, 5Â-GTCATACTGTCACATTGAAACATACAGTAATAC-3Â.In this procedure, three cDNAs that were regarded as the products of aberrant splicing were newly obtained inaddition to the former...
... mechanisms of thiamin diphosphate enzymes:defining states of ionization and tautomerization of the cofactor at individual stepsNatalia S. Nemeria, Sumit Chakraborty, Anand Balakrishnan and Frank ... forthe presence ofthe conjugate base in the activated or unactivated forms of YPDC [51].It is important to emphasize that determination of the state of ionization and tautomerization of enzyme-bound ... substitution of either His (a presumed generalacid ⁄ base catalyst) or Ser (a nucleophilic catalyst) byAla in the well-characterized Asp-His-Ser catalytictriad of subtilisin leads to an approximate...
... faultor none of them. (Garcilaso de la Vega 1966: 331; my emphasis) The above quotation from Garcilaso is unfortunately not withoutambiguity. What can the famed author ofthe Comentarios reales ... interpretation of khipu recordshave maintained not only that the khipu served as an arrangement of visual and tactile ‘‘cues’’ forthe recall ofthe information retained in the memory of its maker, ... 58–60 on the use of formulas in ancient Greek and1960s–1970s Yugoslavian oral narration), it is reasonable to supposethat formulas may have formed important components ofthe narrativestrategies...
... QuickChangemethod (Stratagene, La Jolla, CA, USA). The primersequences were as follows: 5Â-GG GGT GTT ATG GTTTCC CATCGA TCT GAA GAA A CT GAA GAC (G376E)and 5Â-CCA TTC TTG AAC GTT TTA AAC GGT GATTCC ... 1). The s20,wvalue for the G376E variant was measured at four proteinconcentrations and the Kdvalue for this variant wascalculated; Kdwas increased by a factor of 103(Table 1).Thermal ... observed a result ofa change in the size or charge ofthe amino acid? Variants withalanine at these positions were also studied. For tem-perature denaturation, any change at these positionswas...