flow cytometric detection of human regulatory t cells
Báo cáo y học: "The potential of human regulatory T cells generated ex vivo as a treatment for lupus and other chronic inflammatory diseases" pot
... Evidence that the T cell repertoire of normal rats contains cells with the potential to cause diabetes Characterization of the CD4+ T cell subset that inhibits this autoimmune potential J Exp ... death of some T cells [48,49], while others observed that this cytokine protected T cells from apoptosis [50,51] We favor the hypothesis that TGF-β promotes the death of mature Th1 and Th2 cells ... other groups have observed that TGF-β inhibits the differentiation of T cells to Th1 or Th2 subsets [36,37] The initial observation that TGF-β is an IL-2-dependent differentiation factor for regulatory...
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Báo cáo y học: "Signatures of human regulatory T cells: an encounter with old friends and new" pdf
... 5'-CGA AGG GTC TCC GCG GGG TCA CAT-3' TNFRSF1B 5'-GTA GCC TTG CCC GGA TTC TGG-3' 5'-ACC CTG CCC CTG CTC TGC TA-3' TRAF1 5'-GGG GCA TAA ACT TTC CTC TTC C-3' 5'-TTT GGG GTT ATA CAT TGC TCA GTG-3' LGALS3 ... CTLA4 5'-TGC AGC AGT TAG TTC GGG GTT GTT-3' 5'-CTG GCT CTG TTG GGG GCA TTT TC-3' CCR7 5'-TGG CCT GCA GGA AAC ACC-3' 5'-GGG AGA CTT CTT GGC TTG GTG AG-3' RPS9 5'-CGC AGG CGC AGA CGG TGG AAG C-3' ... downregulation of FHIT also contributes to the inactive status of TP53 Based on our data, we suggest that destabilization and thereby inactivation of TP53 provokes a shift in TReg cells from apoptotic sensitivity...
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Báo cáo y học: " Toll-like receptors in cellular subsets of human tonsil T cells: altered expression during recurrent tonsillitis" ppsx
... recurrent tonsillitis, negative cultivation test) None of the patients displayed symptoms of acute infection at the time of surgery, and none of them had received any antibiotic treatment for at least ... raises questions regarding the role of TLRs in T cell activation both with and without APCs Thus, further functional studies of the relevance of TLR stimulation on T cells are of great importance ... visualization of proteins, the sections were treated with xylene to remove the paraffin and rehydrated using ethanol To facilitate binding of the Abs, the sections were treated with Target retrieval...
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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
... soluble factors in the supernatant inhibit the maturation of fusion cells and have a negative impact in the stimulation of T cells To determine the induction of WT1-specitic CD8+ T cells, a pentameric ... as the percentage of the total population of CD8+ T cells that were double positive (CD8+pentamer+) Cytotoxicity assays The cytotoxicity assays were performed by flow cytometry CTL assay that ... http://www.translational-medicine.com/content/6/1/51 Figure The ability of vaccination with autologous FCs to stimulate T cells The ability of vaccination with autologous FCs to stimulate T cells A, Nonadherent PBMCs obtained...
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Báo cáo y học: " CD39+ Regulatory T cells suppress generation and differentiation of Th17 cells in human malignant pleural effusion via a LAP-dependent mechanism" pps
... suggesting that these proinflammatory cytokines might affect the generation and differentiation of Tregs or/and Th17 cells in MPE To evaluate the contribution of cytokines to the numbers of pleural ... generation and differentiation of Th17 cells It was quite well documented that various cytokines contribute to the generation and differentiation of Tregs or Th17 cells In the present study, ... by flow cytometry (B) The representative dot plots of CD39+Tregs and Th17 cells detected in naïve CD4+ T cells after culturing in presence both IL-1b and IL-6 (C) The representative dot plots of...
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báo cáo hóa học:" Generation in vivo of peptide-specific cytotoxic T cells and presence of regulatory T cells during vaccination with hTERT (class I and II) peptide-pulsed DCs" pot
... concluded that hTERT p540 is not expressed or is cryptic on the surface of tumour cells and that immunization of cancer patients with hTERT p540 leads to the production of T cells that not recognize tumour ... with hTERT-pulsed DCs homing of the tumour-specific T cell populations to tumour sites contributes to the effectiveness of the antitumour immunity generated [85] Unfortunately, due to the limited ... substantial generation of tetramer+, CD8+, T cells In fact, the responses generated to the two DC preparations were atypical only in this particular patient The remaining patients showed the pattern...
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Báo cáo hóa học: "IL-2 as a therapeutic target for the restoration of Foxp3+ regulatory T cell function in organ-specific autoimmunity: implications in pathophysiology and translation to human disease" doc
... facilitate hematopoietic stem cell transplantation (HSCT) Studies in such patients indicate that Treg cells increase in response to the treatment, and that this effect seems to be increased with ... target organ may disturb the positive feedback loop that controls Foxp3 stability, such that Treg cells convert to Teff cells with a high diabetic potential Moreover, Komatsu et al noted that ... function and state of activation, may not accurately reflect the status and behavior of their counterparts localized in the target organ In this latter regard, there is experimental evidence that...
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Báo cáo y học: "Nature of Regulatory T Cells in the Context of Allergic Disease." ppsx
... et al, Regulatory T Cells in the Context of Allergic Disease thus preventing the activation of mast cells and basophils IgG4 has been shown to reduce the IgE-mediated degranulation of these cells ... required to elucidate the in vivo role of these cells and their subsets Effects of SIT on Dendritic Cells To understand the mechanisms of action of SIT, some cardinal steps should be explained The ... Treg cells, account for to 10% of peripheral CD4+ T cells and inhibit the activation of effector T cells in the periphery.38,39 FOXP3, the transcriptional regulator expressed on Treg cells, acts...
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Báo cáo y học: "A paragon of self-tolerance: CD25+CD4+ regulatory T cells and the control of immune responses" pps
... characterisation of the surface profile of TR cells has revealed them to be quite distinct from conventional naïve effector T cells Aside from the constitutive expression of CD25, TR cells show elevated ... quite different from that of naïve T cells However, it should be noted that most, if not all, of their apparently characteristic molecules are upregulated during conventional T cell activation This ... confirmation of whether such peripherally generated regulatory cells are contiguous with naturally occurring TR cells or are simply another T cell activation state will have to await the assessment of...
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Báo cáo y học: "Flow cytometric assessment of the signaling status of human B lymphocytes from normal and autoimmune individuals" doc
... STAT4 was much more sensitive than Western blotting and that total STAT4, in addition to pSTAT4, could also be detected by intracellular flow cytometry These four studies [7–10] suggest that pSTATs ... variety of circumstances The first study demonstrating phosphorylation of the STATs by intracellular flow cytometry was published in 1999 and showed detection of pSTAT1 following in vitro activation ... blotting and multiparameter intracellular flow cytometric analysis of pTyr Importantly, cryopreservation did not affect intracellular flow cytometric analysis of pTyr when staining was directly...
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Báo cáo y học: "CD25brightCD4+ regulatory T cells are enriched in inflamed joints of patients with chronic rheumatic disease" potx
... enrichment of regulatory T cells was seen Thus, the simple extrapolation of the animal data into future therapeutic strategies that aim at reconstituting this population does not seem to hold true ... in the joint These features were apparent in the vast majority of the patients despite the different treatments they received, indicating that the anti-rheumatic drugs that patients receive not ... enrichment of CD25brightCD4+ T cells in the joint of patients with rheumatic disorders suggest that the immune system is actively attempting to control the inflammatory responses by recruiting regulatory...
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Báo cáo y học: "Regulating the immune system: the induction of regulatory T cells in the periphery Jane H Buckner1 and Steven F Ziegler2" pot
... suggested that the TR responses are specific for selfantigens It is thought that TR cells in mice represent those thymocytes with the highest affinity for self-peptide but that are below the threshold ... Tr1 cells [74], extends this paradigm The fate of the TR cells induced at the site is not known — whether these cells exist only transiently then die, whether they persist in the body as TR cells, ... suggests a larger question: Do TR cells represent a lineage of T cells or a state of activation that may be achieved by any T cell under the appropriate conditions of activation? The induction of TR...
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Báo cáo y học: "The role of regulatory T cells in antigen-induced arthritis: aggravation of arthritis after depletion and amelioration after transfer of CD4+CD25+ T cells" doc
... treated with control IgG These data imply that a substantial proportion of the T- cell compartment is still activated 14 days after intra-articular antigen challenge in the absence of Treg cells ... at the site of inflammation is also an important part of the activity of Treg cells In this regard, data on the curative effects of Treg cells in experimental disease models are conflicting To ... clearly demonstrates that their suppressive effect is not strictly limited to autoreactive T cells Taking into consideration that Treg cells are also critically involved in the control of immune responses...
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Báo cáo y học: "Role of regulatory T cells in experimental arthritis and implications for clinical use" potx
... treatment decreases the infiltrate in joints Furthermore, RA patients relapse shortly after withdrawal of anti-TNF-α [19] and thus, despite the dampening of joint inflammation and the reinstatement of ... active RA before treatment with anti-TNF-α were unable to suppress proinflammatory cytokine secretion from activated T cells and monocytes After anti-TNF-α treatment the ‘hibernated’ peripheral ... encephalomyelitis have highlighted the potential of this option by genetic manipulation of T cells [21,22] However, this might not be an easy road to follow in humans because not all autoantigen-specific T...
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Báo cáo y học: "Indoleamine 2,3-dioxygenase-expressing dendritic cells are involved in the generation of CD4+CD25+ regulatory T cells in Peyer''''s patches in an orally tolerized, collagen-induced arthritis mouse model" ppt
... immunity or immune tolerance; this determination is based on the maturation or activation state and the subset of DCs, and cytokine profiles in the microenvironment at the time of antigen uptake ... addition of 1-MT, an IDO inhibitor These findings suggest that the functional activities of IDO from tolerized mice could affect the T- cell response In the resting state, autoreactive T cells ... unresponsive to T- cell receptor stimulation, to produce neither T helper nor T helper cytokines, to express TGFβ, and to inhibit normal T- cell proliferation in vitro [42] Different to this regulatory T- cell...
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Báo cáo y học: "Japanese Encephalitis Virus wild strain infection suppresses dendritic cells maturation and function, and causes the expansion of regulatory T cells" doc
... replication DCs infected with P3 attenuated allostimulatory activities to T cells To test whether P3 infection will impair the ability of DCs to activate allogeneic naïve T cells, the direct effect ... acute or chronic infection because of the defect of APCs function for activating T cell [21-23] Therefore it suggested that the impairment of activating of allogeneic naïve T cells of P3 infected ... UV-P3-stimulated DCs did not alter the expansion of the Treg, as well as the mock-treated DCs Discussion Most studies conducted to evaluate the pathogenesis of JEV infection have noted the interaction...
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Báo cáo y học: "Two discrete events, human T-cell leukemia virus type I Tax oncoprotein expression and a separate stress stimulus, are required for induction of apoptosis in T-cells" pptx
... WT-4 C816 ml TL-O u Cd treatment Zn treatment TL-S 1.4 1.2 0.8 0.6 0.4 0.2 WT-1 C ell V iability R atio (treated / untreated) b) c) 0.8 Cd treatment Zn treatment 0.6 JPX- t Jurka od ILT-H WT-4 ... ml TL-O TL-S u 0.4 0.2 WT-1 C ell V iability R atio (treated / untreated) 1.2 Figure Quantitation of apoptosis and viability in Jurkat and JPX-9 cells treated with ZnCl2 or CdCl2 Quantitation of ... with these drugs relative to that of untreated cells http://www.retrovirology.com/content/1/1/7 extraction buffer and sonicated Protein concentrations were determined using the Bio-Rad protein...
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Báo cáo y học: "haracterization of regulatory T cells in urban newborns" pptx
... and the R system for statistical computing [27] Results Subject characteristics The subjects in this study consisted of a subset of newborns and mothers enrolled in URECA at the Boston study site ... no statistical differences in baseline characteristics of newborns with and without proliferation data (Table 1) Although of the infants were admitted to the ICU, none of them were intubated ... differences in the numbers of CD25+bright cells present in CB and PB, we next sought to establish whether or not these CD25+bright cells expressed distinct patterns of differentiation/activation markers...
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Báo cáo sinh học: " Protection against the allergic airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice" docx
... directly to DCs may a new alternative of therapies for patients with allergic asthma These findings suggest that spleen DCs and Foxp3+Tregs prevents the generation and activation of Th2 effector cells ... mature phenotype and express a range of co-stimulatory molecules that are intermediate between immature and mature DCs, resulting in tolerogenic interaction with T cells in SIT We found that the ... novel pathway of regulation of type immunity in asthma Competing interests The authors declare that they have no competing interests Authors' contributions YL performed all analyses and wrote the...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... subjected to atmospheric levels of CO2 MTT assay to quantify hESC survival rate after exposure to low temperature The MTT assay [3] was performed to quantify the survival rate of hESC after exposure ... exposure to low temperature, a 6-well culture dish with the same 125 density of hESC colonies was subjected to the MTT assay on the same day that the other dishes were being exposed to low temperature ... with PBS followed by air-drying The MTT-formazan products were extracted in the dark at room temperature with ml of DMSO One hundered microliters of the supernatant was later transferred into...
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