... and barley a- amylases are thus also presented.MATERIALS AND METHODSSUMAsoftware: subsite mapping of amylasesThis software calculates the apparent binding energies on the basis ofthe measured ... PPA, an a- amylase studied by us earlier. Also an attempt has beenmade to use this program for subsite mapping of other a- amylases found in the literature. Evaluations of subsitemaps of rice and ... Mapping of a- Amylases) is freely availablefor research and educational purposes via the Internet(E-mail: gyemant@tigris.klte.hu). The advantages of this program are demonstratedthrough a- amylases...
... phosphorylation. The data presented here suggest that the pro-domain of BMP-2 can alter the signalling properties ofthe growth factorby modulating the ability ofthe mature part to interact withthe ... was assessed at a single timepoint (30 days), and neither the pharmacokinetics of proBMP-2 nor the kinetics of bone formation were anal-ysed. Thus, the fate of administered proBMP-2 in the animal ... in these experiments.Table 1. Kinetic constants for interaction ofthe ECD of BMPR-IA with the growth factors. Association (k a ) and dissociation (kd) ratesfor the ligands withthe ECD and...
... ringare Asn53, Gln70, Trp78, Ser114, Arg180 and Asp181(Fig. 3C). The interactions ofthe blades in the axisarea are of polar character. The water channel in the apo formofthe enzyme runs along the ... probably a consequence of a mistake incorporated by the TaKaRa Ex TaqÔ poly-merase and did not affect the enzymatic activity.MS The molecular mass ofthe recombinant b-fructofuranosi-dase was ... Technical University of Lodz, PolandIntroductionBifidobacteria are found in human and animal gastro-intestinal tracts, as well as in the oral cavity and the vagina [1]. They are among the first bacteria...
... repair factor XP-B relocalizedto damaged areas, where it gave a uniform staining(Fig. 5, upper panel). Staining with an antibody againstnucleotide excision repair factor XP -A gave a compar-able ... 5–10% ofthe input. In all panels, hyperphosphor-ylated RNA pol IIO is indicated witha solid arrow and hypophos-phorylated RNA pol IIA with an open arrow.DNA polymerase e associates with RNA ... performed and analysed as above. Hyperphosphorylated RNAPol IIO is indicated witha solid arrow and hypophosphorylated RNAPol IIA with an open arrow. A. K. Rytkoănen et al. DNA polymerase...
... or was obtained from Oriental Yeast Co. (Lot21003805) (Osaka, Japan) and proCPY was prepared as the same manner as CPY, with minor modifications. Dgly CPYand Dgly proCPY, in which the asparagine ... indicating that the thermalunfolding process ofthe precursor form follows a two-statetransition. The ratio ofthe unfolding enthalpy (DHcal)tothevan’t Hoff enthalpy (DHv) was 1.05 (DHcaland ... for the baseline.DHcaland DHv(van’t Hoff enthalpy) were determined from the scanned data using the ORIGINsoftware program(version 4.0) (MicroCal Inc.).Qualitative thermodynamic parametersfor...
... ofa drop U of minimal generation. It then follows the boundary of U along a nontrivial arc I. Finally, it returns along the boundaries of another chain of descendants of U until it reaches a ... case, the proof of Theorem A shows that the boundary of ∆θis a David circle, i.e., the image ofthe round circle under a David homeomorphism. It can be shown that, unlike quasiconformal maps, ... is the theorem of David on integrability of certain Beltramidifferentials with unbounded dilatation [Da]. David’s integrability conditionrequires that for all large K, the area ofthe set of...
... B=MICBị=nwhere A and B are the MICs of drug A and drug B in the combination, MIC A and MICBare the MICs of drug A and drug B alone, FIC A and FICBare the FICs of drug A and drug B and n is the number of ... purchased from Avanti Polar Lipids (Alabaster,AL, USA). FITC-Ds were purchased from Sigma. All otherchemicals were reagent grade. For antimicrobial assays, the commercially available quality ... thank M. Simmaco for use ofthe facilities andplatforms available in the DiMA Unit ofthe Sant’An-drea Hospital. This work was funded in part by ItalianMinistero dell’Universita`e Ricerca...
... catalyst. This results in formation of only a few of SiNWs. The reaction temperature is usually decided withthe consideration of the catalyst type. According to the binary phase diagram ofa ... (7)). The above factors easily cause a low-crystalquality of as-received SiNWs even amorphous SiO2nanowires.3.3. Advantage and disadvantage ofwith and without metal growth The mentioned metal-assisted ... nanowires fabricated with metal can be self-organized while a large scale of samples can be achieved with metal-free catalyst. The growth mechanism comparison between metal- and non-metal assisted...
... oligosaccharide binding fold (OB-fold) proteins. The domain swap pairs one half, b strands 1–3, of Bc-Csp withthe other half, b strands 4 and 5, of a second molecule and serves as proof of an unantici-pated ... sugar–phosphate backbone of the nucleotide in subsite 6 and the formation of the alternative subsite 7 are a consequence ofthe inaccessi-bility of Trp8 in this crystal form. In addition to their ... Mutational analysis ofthe puta-tive nucleic acid-binding surface ofthe cold-shockdomain, CspB, revealed an essential role of aromaticand basic residues in binding of single-stranded DNAcontaining...
... Spectrophotometric characterization ofthe reactions of DA analogs with a- Syn and some amino acids. (A) UV-vis spectra of a- Syn with DA, CA, HQ and Q after reaction for 24 h. The a- Syn alone sample was as a control. ... differentmolecular masses, while the intact a- Syn gives a majorpeak witha molecular mass of 14 458 Da. Table 1 dis-plays the molecular masses and the proposed pattern of the monomeric adducts by these reactions. ... reactions. Reaction of a- Syn with these DA analogs gives a group of adducts with molecular masses larger than intact a- Syn (expectedly 14 460 Da). For the Q reaction, the additional mass is an exact multiple...
... heterozygosity of the GPIIb gene in a Swiss patient with Glanzmann’s thrombasthenia.Br.J.Haematol.102, 918–925.49. Tao,J.,Arias-Sagado,E.G.,Gonzalez-Manchon,C.,Iruin,G.,Butta, N., Ayuso, M.S. & Parrilla, ... Corp.) at 37 °C, with continuous stirring at 1200 r.p.m. The maximalaggregation, achieved within 3 min after addition of the agonist, was determined and expressed as a percentage of 100% light transmission ... functions, has led to extensive research in the development of small RGD-containing peptides as anti-thrombotic agents. Elucidation ofthe pharmacophoricnature ofthe Asp and Arg side-chains allowed...
... of data and revision ofthe manuscript. AG par-ticipated in the study design, analysis and interpretation of data and drafting the manuscript. BM participated in the acquisition of data and the ... individual symptoms provided additionaluseful information on patient satisfaction with BPH phar-macotherapy above and beyond what was already pro-vided by global satisfaction. A draft ofthe questionnairewas ... perceptions of change and their satisfaction with that change. Using datafrom a randomized clinical trial in the USA, the resultssupport the reliability, validity and responsiveness tochange of the...
... words,occlusal traits that affect dental appearance and have animpact on participants' daily lives may not be captured byIOTN. In addition, DAI has many more measures of malocclusion affecting the ... simultaneously, and thuscollect information about wider effects of oral health ondaily living. The main advantage of generic measures isthat they allow comparison of various domains of quality of ... forindividuals who have a DHC grade of 4 or 5, or grade 3 with an AC of 6 or above. All other cases were thereforeclassified as having no need. For DAI, 10 occlusal traitswere assessed and a score was...
... operator in Banachspaces and the concept ofthe generalized nonexpansive mapping in Banach spaces.Honda et al. [11], Kohsaka and Takahashi [12] also studied some properties for the gen-eralized ... solutions of an equilibrium problem and the set of fixed points ofa relatively nonexpansive mapping in Banach spaces. Ibaraki and Taka-hashi [10] introduced a new resolvent ofa maximal monotone ... YJC and ZW carried out the studies on nonlinear analysis and applications, wrote this article together andparticipated in its design of this paper. All authors read and approved the final manuscript.Competing...
... conditions on the coefficients are given to guaranteethat all the species are permanent. It is shown that these conditions are weaker than those of Liaoet al. 2008.1. IntroductionTraditional Lotka-Volterra ... 4 Advances in Difference Equations2. Proof of Theorem 1.2 The following lemma can be found in 10.Lemma 2.1. Assume that A& gt;0 and y0 > 0, and further suppose that(1)yn 1≤ Ayn ... exponentially for 2 ≤ i ≤ N, and uit → X∗, where X∗is a certain solution of a logistic equation. Teng 8 and Ahmad and Stamova 9 also studied the coexistence on a nonautonomous Lotka-Volterra...