... measure the mobility ofthe components in the PGCs The medaka embryo was peeled off the chorion, andthe segment containing the part of PGCs was excised for observation by microscopy and FCS ... of OlvasGFP reveals the dynamic nature ofthe nuage Schematic diagram ofthe preparation of PGCs of medaka specimen (A) OlvasGFP was expressed in the medaka PGC at stage 24 and localized in the ... and FRAP analyses of OlvasGFP in the PGC FCS was used to measure the movement of OlvasGFP into the cytosol out ofthe nuage region ofthe PGC Representative correlation curves are shown (A) The...
... A2 and pET A3A4A5 A1 and pET A3A4A5 A1A2 and pET A4A5 A1A2 and pET A3A4A5 On the basis ofthe results of this assay and mutational studies on the a subunits of other dioxygenases [18–26], the ... Hence, AtdA1 and AtdA3 are critical for the activity ofthe enzyme and provide good starting points for the study ofthemolecular determinants ofthe substrate specificity and activity of AtdA FEBS ... defining the substrate-binding pocket ofthe a subunit, AtdA3, and applied saturation mutagenesis to these residues to probe themolecular determinants ofthe activity and specificity ofthe enzyme...
... wild-type ALP I On the other hand, the Tm of D266N/Y269A-ALP I increased by only °C The denaturation temperature indicates the stability ofthe structure ofthe protein The difference ofthe increments ... on the surface On the other hand, anionic surfactants such as SDS and LAS bind to the surface regions of protein, andthe surface regions would be unfolded and removed from the core structure of ... 60% ofthe original activity after h, and 30% after h The D266NALP I showed 40 and 20% ofthe original activity after h and h of incubation, respectively The stability of Y269A/A271T/Q272R- and...
... location of amyloidogenic mutations and position of b-strands (A) The structure ofthe polypeptide backbone ofthe two chains (purple and blue) is shown along with the location ofthe N-terminus and ... within a tissue and that the distribution of TTR deposition reflects the presence of these endogenous factors In the case ofthe Ab protein of Alzheimer’s disease, a number of proteins and factors ... Dislocation ofthe C and D strands from their native edge region may result in the formation of a new interface involving A and B strands which is open for intermolecular interactions and consequently,...
... clinical care ofthe patients and were an important step towards the understanding ofthe mechanisms of this disorder This minireview summarizes the advances in CCM molecular genetics andthe remaining ... loss, within affected cells, ofthe two alleles of a given CCM gene Loss of one ofthe alleles (first hit) would be the result of a germline mutation and loss ofthe second allele (second hit) ... the identification of CCM genes, a number of recent biochemical in vitro studies and in vivo CCM animal model studies have helped to unravel the functional roles of these proteins and are be the...
... amount of carbon and nitrogen, indicating the presence of protein(s) However, the protein was not isolated or identified because the focus of that study was identifying themolecular form ofthe ... including its sensitivity to a wide variety of chemically dissimilar compounds and even to the changes in the conformation of major IM proteins that are capable of modifying the surface charge ofthe ... [36], the ‘calciphorin’ isolated from liver and two ‘calciphorin’ isolates from calf heart were quite different in terms of their estimated molecularmassand other parameters To the best of our...
... Evolution oftheMolecular Biology of Brain Tumors andthe Therapeutic Implications high grade gliomas The structure and compounds ofthe ECM ofthe brain tissue have many specific differences from other ... mortality ofthe patient Moreover, the presence ofthe blood-brain barrier (BBB) limits the passage of a wide variety of anticancer agents The high incidence of recur‐ rence and poor prognosis of malignant ... infiltrate the enviro‐ ment and form it similar to the tumor tissue The process ofthe peritumoral invasion de‐ pends on the confrontation zone ofthe tumor cells andthe non-neoplastic cells and ECM...
... characterized the contribution of these assay components by omitting them individually In the absence of ferredoxin the reaction rate decreased to 46.5% ofthe complete reaction while the absence of ascorbate ... indicate the direction ofthe major changes in absorption over the course ofthe measurements (C) Michaelis– Menten plot ofthe PsHO1 reaction for heme concentrations of 1, 2, 5, 10 and 20 lM ... three-dimensional model ofthe structure of pea HO was produced on the basis ofthe alignment ofits amino acid sequence (minus the N-terminal transit sequence) with HOs from human, rat andthe bacteria,...
... end ofthe range andcompound across the 60 cell lines, andeach column represents the pattern of sensitivities of a par- 6.86% censored at the low-concentration end ticular cell line to the compounds ... row-wise mean and dividing by its rowwise standard deviation; took the inner product ofthe normalized A andthe transpose ofthe normalized T matrix; and divided each element in the resulting ... ) Σ (Σ ) , and similarly for Vij The Pearson correlation of Uij and Vij gives a measure ofthe similarity in the distributions of drug activity and gene expression Theformula is given by r=...
... etc Another impact ofthe Pleistocene glacials pertained to the connection between the Borneo and Thai-Malay Peninsula (the continent of Asia) The land bridge ofthe Sundaland accelerated the migration ... the rise and fall ofthe sea level severely altered the area of landmass of Southeast Asia, i.e., the emergence and Species Occurrence, Hybridization and Speciation in Postglacial East and South ... temperate and tropical Asia, particularly from the mainland of China Due to the variable topography andthe short distance from the continent of Asia, the island forest species are diverse and similar...
... Tsix The role of DNA methylation Figure Transcription of Xist and Tsix on the X chromosome The coding sequences of Xist and Tsix overlap on opposite strands ofthe X-chromosome DNA effect: the ... selected as the XA andthe other as the XI in each cell In addition to having opposing roles in random choice, Xist and Tsix also negatively regulate each other in ES cells Xist and Tsix are ... initiated A second cause of non-random X-chromosome inactivation is the selective death of cells that inactivate the incorrect number of X chromosomes: because the fates ofthe X chromosomes are...