filtration and normalization of microarray data
Báo cáo y học: "Elevated extracellular matrix production and degradation upon bone morphogenetic protein-2 (BMP-2) stimulation point toward a role for BMP-2 in cartilage repair and remodeling" pps
... production of aggrecan, 35SO42- incorporation into patellar and tibial cartilage was assessed The cartilage of the patella and the tibia was isolated 3, 7, and 21 days after viral injection and incubated ... induction of tendon and ligament in rats by growth and differentiation factors 5, 6, and 7, members of the TGF-beta gene family J Clin Invest 1997, 100:321-330 Ducy P, Karsenty G: The family of bone ... Amsterdam, The Netherlands) Quantitative PCR Patellar and tibial cartilage was stripped off the joint as previously described [16] (time points 3, 7, and 21 after injection of the adenovirus; n...
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Báo cáo y học: "High level expression of apoptosis inhibitor in hepatoma cell line expressing Hepatitis
... stimulation of HBV viral proteins indeed changes the expression of apoptosis profile of host cells, which probably results in the carcinogenesis of liver cells Materials and methods Cell lines and isolation ... organization and physical map of the human inhibitors of apoptosis: HIAP1 and HIAP2 Mamm Genome 1999, 10: 44-48 44 Yang L, Cao Z, Yan H, Wood WC Coexistence of high levels of apoptotic signaling and inhibitor ... processing of procaspase-3, procaspase-6, and procaspase-7 by binding and blocking the activity of caspase-9 Increasing evidence demonstrated that IAPs are up regulated in many human tumor types and...
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Tài liệu Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR pptx
... expression of STMN1, AMY2A, and ISL1, and positively correlated with S100P, RAB25, and CDH1 The loading number of component (PC3) was negatively correlated with the expression of RAB25 and AMY2A, and ... The microarray results were deposited in the CIBEX microarray database (accession CBX 100) Quantification of 19 candidate genes by qPCR Using qPCR, we quantified the expression of the 19 candidate ... gave a total of 152 data points (19 × 8) from each of the DNA microarray and qPCR analyses Increases or decreases in 118 data points from qPCR were consistent with those from the DNA microarray...
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Tài liệu Báo cáo khoa học: MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor pdf
... were predicted by two of the algorithm programs (pictar and targetscan release 5.1) and were also downregulated in our cDNA microarray were selected as candidate targets of miR-23a Among these ... because of its higher fold of upregulation in gastric adenocarcinoma tissues Although the extent of upregulation of miR-23a in the four pairs 3730 of gastric tissues showed great variance because of ... the regulatory effects of miR-23a on the expression of IL6R by RT-PCR and western blot, and found an inverse correlation between miR-23a and IL6R expression at both the mRNA and protein levels To...
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Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
... Ministry of Education, Science, Sports, and Culture of Japan References Taketani S (2005) Aquisition, mobilization and utilization of cellular iron and heme: endless findings and growing evidence of ... synthesis and heme degradation is responsible To explore the role of free heme in HO-1 and HO-2 expression, we treated HeLa and HepG2 cells with SA and determined the expression levels of HO-1 and ... of HO-1 and HO-2 mRNAs and proteins in HeLa and HepG2 cells (Fig 5) Thus, the induction of HO-1 is due to the selective repression of HO-2 expression achieved with HO-2 siRNA 5336 Knockdown of...
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Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf
... effects of hypoxia on the expression of HO-1 and HO-2 in human cell lines of Reduced expression of heme oxygenase-2 A B Fig Effects of hypoxia on expression of heme oxygenase (HO)-1 and HO-2 ... reduction of HO-1 and HO-2 protein levels, we analyzed the effects of hypoxia on the expression of HO-1 and HO-2 mRNA in YN-1 erythroleukemia cells, which express detectable levels of both HO-1 and ... expression levels of HO-1 and HO-2 mRNA after 48 h of incubation in the two cell lines (Fig 2A) Likewise, hypoxia decreased the levels of HO-1 and HO-2 proteins by more than 60% and 30%, respectively,...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
... stelleate cells and synthesis of collagen [36,37], the in vitro data presented here may be relevant for understand some of the complex mechanisms responsible for the development of fibrosis and cirrhosis ... consumption and to examine the possible role played by cells and molecules of the immunological system in hepatic injury and repair Understanding the interdependence between the metabolism of iron and ... loading of the liver epithelial cell line HepG2 influenced the expression of immune regulatory molecules known to function as ligands of selected subsets of T cells, such as MHC class I and II,...
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Tài liệu Báo cáo khoa học: Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers ppt
... lg of polyclonal anti-eEF1A (lane 4), with nuclear proteins and 0.9 lg of polyclonal anti-eEF1A (lane 5), with nuclear proteins and lg of total CRS (lane 6), with nuclear proteins and 0.9 lg of ... presence of lg poly(dIdC) and lg of CT as competitors, as described in the Materials and methods After 25 of incubation at room temperature, the samples were cross-linked by UV exposure and then ... 3151.9, 3980.3 and 4108.5) and demonstrated the absence of seven of the eight amino acid substitutions described for PTI-1 (Ala65Met, Glu66Gln, Arg67Ser, Lys100Gln, Arg247Gly, Ala281Gly and Arg423Cys,...
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Tài liệu Báo cáo khoa học: Insulin/protein kinase B signalling pathway upregulates metastasis-related phenotypes and molecules in H7721 human hepatocarcinoma cell line pptx
... stained and countered as described above, and the number of cells that had migrated to the lower side of the matrigel-coated filter was a measure of the invasive activity of the cells Inhibition of ... of insulin and LY294002 on the expression of SLex on H7721 cells (A) Flow cytometry (–) Control, without the addition of the first antibody (B) Calculation of relative expression of SLex The data ... protein, and AP-1, b-catenin/Tcf/LEF are the downstream signalling molecules of PKB or GSK-3, and PKB can induce initiation of mRNA translation through phosphorylation of 4E-BP and activation of eIF-4E...
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Báo cáo khoa học: Adenine nucleotides inhibit proliferation of the human lung adenocarcinoma cell line LXF-289 by activation of nuclear factor jB1 and mitogen-activated protein kinase pathways doc
... P2Y11 and P2Y13 and for the P2X4 receptor, whereas expression of the P2Y1, P2Y4, P2Y12, P2X3 and P2X7 receptors could not be detected (Fig 2) Inhibition of growth and cell cycle progression of LXF-289 ... potency of ATP and ADP in the inhibition of LXF-289 cell proliferation Cell cycle analysis revealed that inhibition of proliferation of LXF-289 cells by ATP and ADP was mediated by retardation of ... addition of 100 lm ATP enhanced the antiproliferative potency of cisplatin 3-fold, of etoposide 2-fold and of paclitaxel 2.5-fold, with resulting IC50 values of 4.7 ± 0.8 lm, 9.3 ± 2.3 lm and 6.8...
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Báo cáo khoa học: The in vitro effects of CRE-decoy oligonucleotides in combination with conventional chemotherapy in colorectal cancer cell lines potx
... previously [23] The acquisition of data was performed within h using a FACSCalibur (BD Biosciences) Five thousand cells were analysed for each data point, and the percentages of cells in sub-G1 (apoptotic ... loss of CDK function, and the generation of cells with senescence characteristics In the first part of the investigation, we determined the effect of continuous exposure to CDO on cell viability and ... (iii–iv) The results of densitometry analyses are given in (A) and (B), and are expressed as a percentage of each individual control In summary, these data provide a possible model of drug interaction...
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Báo cáo khoa học: 3 Cdt1 and geminin are down-regulated upon cell cycle exit and are over-expressed in cancer-derived cell lines potx
... h-geminin and h-actin, extension at 72 °C for min; 26 cycles were used for the amplification of hCdt1 and h-geminin cDNAs and 20 cycles for the amplification of h-actin cDNA The number of cycles ... open reading frames of mouse Cdt1 and geminin as templates and the T3 and T7 polymerase, respectively, while sense probes served as negative controls and were generated using T7 and T3 polymerase ... indicated by an asterisk The position of migration of the 66-kDa molecular mass marker band is indicated at the left of the Cdt1 blot Fig Cdt1 and geminin protein levels and localization at the transition...
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Báo cáo Y học: Proteasome-driven turnover of tryptophan hydroxylase is triggered by phosphorylation in RBL2H3 cells, a serotonin producing mast cell line pptx
... Addition of both okadaic acid and MG132 caused the immunoreactive band to be twofold thicker than the control band (see plot profiles of WB, right-most patterns), however, no discrete bands of 32 ... under ordinary conditions and prepared in the presence of mM ATP, mM dithiothreitol and 0.25 M sucrose to minimize the dissociation of proteasomes and possible disruption of lysosomes [41] Based ... of structural proteins during the assembly of the head of bacteriophage T4 Nature 227, 680–685 Bradford, M.M (1976) A rapid and sensitive method for the quantitation of microgram quantities of...
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Báo cáo Y học: The SK-N-MC cell line expresses an orexin binding site different from recombinant orexin 1-type receptor pptx
... characterization of the receptor binding mode of orexin A and B and provide important information for pharmacological and biochemical investigations The observed A/G exchange in SK-N-MC and NT-2, two ... (specific activity: 2000 CiÆmmol)1; Amersham) and increasing concentrations of the cold ligand orexin B or increasing concentrations of test compounds After 120 of gentle shaking at room temperature ... diluted to a final concentration of 1.0 · 106 cellsÆmL)1 and homogenized using an UltraThurrax After the addition of 5.5 mM glucose, 0.1% BSA and 50 lg bacitracin, 200 ll of this cell suspension was...
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Báo cáo khoa học: Internalization of cystatin C in human cell lines docx
... cytometry and the median of the fluorescence of the cell population was calculated, corrected for the control value and then related to the degree of labelling of the protein used Dashes and rings ... Visualization of acidic compartments by LysoTracker (D) Overlay of B and C, indicating co-localization of cystatin C and LysoTracker B C D taining medium (1 lm) In order to determine the concentration of ... type of cystatin and the cancer cell system studied Overexpression of cystatin C has been shown to alter the metastatic properties of B16F10 melanoma cells [9] and to inhibit the motility and...
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Báo cáo khoa học: Interaction of G-rich GT oligonucleotides with nuclearassociated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines potx
... crosslinking of G-rich GT oligomers Three micrograms of total nuclear extract of CCRF-CEM cells were incubated in buffer C in the presence of ng of the indicated 5¢-end 32P-labelled G-rich GT oligomers and ... solution [24], and the topology of this structure is distinct from the folds of the closely related and well-characterized sequences d(G4T4G4) and d(G3T4G3) [25] Recently, the ability of the G-rich ... protein can be excluded by MALDI TOF analysis of the Coomassie blue band extract [21] and by the absence of a corresponding nucleolin signal in western-blotting of our nuclear extracts (Fig 4A)...
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Báo cáo khóa học: Endoplasmic reticulum-associated degradation of glycoproteins bearing Man5GlcNAc2 and Man9GlcNAc2 species in the MI8-5 CHO cell line docx
... (A, B, C and D) and glycoproteins (E, F, G and H) were analyzed by HPLC as described in Experimental procedures (D) and (H) correspond to the pattern of oligomannosides bound to LLO and glycoproteins, ... analysis of LLO and oligomannoside species bound to newly synthesized glycoproteins during Glc deprivation of MI8-5 cells MI8-5 cells were preincubated in 0.175 mM Glc for 20, 40 and 120 min, and ... the presence of kifunensine, an inhibitor of the demannosylation process Figure 1D,H shows that incubation in the presence of 20 lM kifunensine did not affect the proportion of Man5 and Man9 species...
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Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot
... gift of osteosarcoma q0 and q+cells, Dr I.J Holt for the gift of lung carcinoma q0 and q+ cells and we are grateful to Dr Aubrey de Grey for great help in interpreting and discussing the data ... the CELLQUEST software program (Becton-Dickinson) Fig GSH and GSSG concentrations and ratio of GSH2 : GSSG in q+ and q0 cells from osteosarcoma (bone), rhabdomyosarcoma (muscle) and lung carcinoma ... fluorescence was measured as an index of steady-state levels of ROS under basal and stress conditions (Fig 9, Table 1) In the presence of glucose and 10% serum (standard growth conditions), the fluorescence...
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Báo cáo Y học: GPI-microdomains (membrane rafts) and signaling of the multi-chain interleukin-2 receptor in human lymphoma/leukemia T cell lines doc
... Here, a minimum of 5000 pixels of digital cell images were analyzed in terms of bleaching kinetics and the efficiency of FRET was calculated from the mean bleaching time-constants of the donor dye ... light and heavy chains of class I HLA (used as Ôinternal standardÕ) (right, narrow distribution) and FRET between IL-2Ra and HLA-DR (left, broad distribution) are shown (B) FRET efficiency data ... was defined by both Triton X-100 and Chaps detergents, and found approximately independent of the ligand (IL-2) occupation level of receptors on T cells Analysis of the whole sucrose gradient sedimentation...
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Báo cáo khoa học: An estrogen receptor a suppressor, microRNA-22, is downregulated in estrogen receptor a-positive human breast cancer cell lines and clinical samples pptx
... of human ESR1 3¢-UTR) in human and other mammalian species, including the chimpanzee, rhesus monkey, mouse, rat, dog and rabbit (Fig 2A) The predicted DG of 70 bp 5¢- and 3¢-flanking regions of ... is the median; the upper and lower limits of the box are the 75th and 25th percentiles, respectively, and the vertical bars above and below the box indicate the maximum and minimum values The solid ... 3¢-UTR of ESR1 mRNA Along the 4.3 kb full length of 3¢-UTR of ESR1 mRNA, 59 miRNAs had conserved target sites and partial miRNA families broadly conserved among vertebrates MicroRNA-22 and estrogen...
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