field flow fractionation in therapeutic protein development
field flow fractionation in biopolymer analysis
Ngày tải lên :
29/05/2014, 16:16
... Dukjin Kang Field- Flow Fractionation in Therapeutic Protein Development 73 Joey Pollastrini, Linda O Narhi, Yijia Jiang, and Shawn Cao Assessing and Improving Asymmetric Flow Field- Flow Fractionation ... and Lars Nilsson Assessing Protein- Ultrafiltration Membrane Interactions Using Flow Field- Flow Fractionation 23 Galina E Kassalainen and S Kim Ratanathanawongs ... loading: flowrate ¼ 0.1 ml/min, loop volume ¼ 10 ml, time ¼ Relaxation/focusing: focusing point (distance from inlet, z´) ¼ 5.0 cm, Fcross ¼ ml/min during and 9.9 ml/min during 15 s Elution: Fin...
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Peptidylarginine deiminase (PAD) is a mouse cortical granule protein that plays a role in preimplantation embryonic development docx
Ngày tải lên :
05/03/2014, 17:20
... were incubated at room temperature in M16 culture medium containing anti-ePAD (1:100) for 45 minutes, washed in M16, and incubated 45 minutes at room temperature in M16 containing antiguinea ... is involved in regulating histone arginine methylation by converting methyl-arginine to citrulline [48] In our study, cortical granule PAD appeared to play a role in early embryogenesis since ... binding was reduced by incubation of the extracts with normal rabbit serum at 4°C with constant agitation for 90 minutes To remove any protein- A and Sepharose bead binding proteins before using...
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Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf
Ngày tải lên :
16/03/2014, 12:20
... element binding protein: cellular nucleic acid binding protein Virus Res 58, 73–82 Flink IL & Morkin E (1995) Alternatively processed isoforms of cellular nucleic acid-binding protein interact ... of human La protein increases binding to and shifts the 5¢ TOP-mRNA encoding rpL37 off the polysome distribution suggesting that increased levels 492 of nonphosphorylated La protein may have ... during 15 with different kinase inhibitors at 30 °C and then used to analyse in vitro recombinant CNBP phosphorylation in kinase buffer containing 100 lm ATP and lCi [32P]ATP[cP] at 30 °C for Kinase...
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Báo cáo y học: "Cartilage oligomeric matrix protein is involved in human limb development and in the pathogenesis of osteoarthriti" pptx
Ngày tải lên :
09/08/2014, 07:20
... reducing and denaturing conditions Protein separation was performed applying SDS-PAGE and using systems containing 6% acrylamide in stacking gels and 12% in the separation gel Tris-glycine was ... protease inhibitors NEM (N-ethylmaleimide), EDTA, benzamidine hydrochloride, and amino caproic acid, precipitated in ethanol, washed in PBS, precipitated again, and finally dissolved in PBS containing ... developing joint space The arrowhead indicates the area from which the high-magnification micrograph was taken (inset) The arrowhead in the inset indicates COMP staining (f) At gw 12, COMP staining is...
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Báo cáo y học: "Recent developments in membrane-protein structural genomics" pptx
Ngày tải lên :
14/08/2014, 16:20
... carboxy-terminal location of another 99 proteins out of the 737 proteins was determined by finding their homologs among the 502 experimentally determined proteins When the resulting set of 601 proteins ... readily applied to integral membrane proteins of other genomes An interesting finding by Daley et al [4] is that 57% of the 601 proteins studied have both their amino and carboxyl termini on the cytoplasmic ... membrane proteins in bacteria One of the major concerns for membrane protein production in bacteria is the potential toxicity of these proteins to the host, limiting the ability to express proteins...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
Ngày tải lên :
16/02/2014, 09:20
... implicated in other protein protein interactions, a STAT protein DNA-binding domain and an SH2 domain, which binds phosphorylated tyrosine residues in the context of a longer peptide motif within a ... domains, including an N-terminal STAT protein interaction domain, which strengthens interactions between STAT dimers on adjacent DNA-binding sites, a coiled-coil STAT protein all-alpha domain, ... STAT protein all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and fork-head domain of foxp3 In addition, for stat6 and...
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Tài liệu Báo cáo khoa học: Roles of heat shock factors in gametogenesis and development pptx
Ngày tải lên :
18/02/2014, 04:20
... of the coronal section at E14.5 in (F) (A) At E11.5, HSF2 is expressed in forebrain, midbrain and in hindbrain except for the midbrain, hindbrain midline Surprisingly, the HSF2 expression pattern ... birth in mouse brains [122,124] No HSF4 DNA-binding activity was reported in the developing mouse brain so far However, Hsf4b is expressed in the adult mouse brain, including cerebellum, and in ... tubules, in an Hsp-independent mechanism [86] In contrast, HSF1 is involved in cell death in spermatocytes [86,87] Once again, this Role of the HSF family in development death-promoting effect...
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Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf
Ngày tải lên :
18/02/2014, 17:20
... recombinant OMM-64 variants (rOMM-64-I-V and -C), containing different domains of the protein, to determine the calcium-binding domain of the protein (A) Schematic drawing of the recombinant proteins ... matrix proteins induce aragonite crystals [4–7] We therefore examined proteins contained in aggregates within the otolith matrix and identified a protein contained in the HMW protein glycosaminoglycan ... observed by both ‘Stainsall staining’ and western blotting (arrowheads) All proteins in the EDTA-soluble (OS) and -insoluble (OI) otolith matrix were stained blue using ‘Stains-all’ In these matrices,...
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Tài liệu Báo cáo khoa học: Bone morphogenetic proteins in the early development of zebrafish pptx
Ngày tải lên :
19/02/2014, 00:20
... family, binding to and inhibiting these signaling molecules from binding to their receptors in the extracellular space, thus inhibiting ventralizing activities Of these proteins, Chordin has a ... mfn (C3), indicating that Tld (Tll1) also functions to pattern dorsoanterior structures [29] Twisted gastrulation (Tsg), a BMP-binding protein, initially found in Drosophila, is involved in dorsal– ... regarding the function of tsg in the BMP pathway probably will remain unresolved for some time Other factors affecting the BMP pathway Factors inhibiting BMP2b, other than direct binding proteins...
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Tài liệu Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses doc
Ngày tải lên :
19/02/2014, 12:20
... identification of amino acid residues involved in the protein RNA and protein protein interactions that mediate these functions [9,31,32,39–42] Analysis of the effects of substitutions of key amino acids ... sterically interfere with side-chain packing in the protein s interior Mutations that create cavities in hydrophobic environments generally cause proteins to become less stable [44,45] Using the ... is a powerful tool for using to study cavities in proteins [24,25] The coat protein of bacteriophage MS2 contains two tryptophan residues, thus permitting the use of intrinsic fluorescence as a...
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Tài liệu Báo cáo khoa học: The unique sites in SulA protein preferentially cleaved by ATP-dependent Lon protease from Escherichia coli ppt
Ngày tải lên :
21/02/2014, 03:20
... corresponding to that in Fig The numbers in parenthesis indicate the estimated percentage yields of each peptide after 3-min, 30-min, and 3-h incubations in the presence of ATP and 3-h incubation in ... the inhibitory activity and to presumably constitute a surface for protein protein interaction [13] It is tempting to assume that these initial cleavage sites are strategically placed mainly in ... hydrolysis): fast, over 5% in min; medium, below 5% in min, but over 14% in 30 min; slow, below 5% in 30 Cleavage site: amino-acid residues at P5±P1 and P1¢±P5¢ sites are shown Asterisks indicate major cleavage...
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Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx
Ngày tải lên :
07/03/2014, 03:20
... domains are degenerate 34-amino acid repeats forming anti-parallel a-helices known to be involved in an array of protein protein interactions (generally with non-TPR proteins) [41] Both domains ... CaM-like protein is the most likely mediator of this regulation In an attempt to isolate CaM-binding proteins, Tic32 was identified as the only IEM protein specifically interacting with CaM in a calciumdependent ... (FNR) Interestingly, this membrane binding was found to be reversible, and is assumed to be mediated by a hydrophobic patch on the protein surface, located in the N-terminal half of the protein, including...
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Báo cáo khoa học: Protein transport in organelles: Protein transport into and across the thylakoid membrane pptx
Ngày tải lên :
07/03/2014, 03:20
... for proteinaceous insertion machinery Other singlespanning proteins have also been suggested to use this membrane integration route, including the photosystem II subunits, PsbW and PsbX In describing ... role in thylakoid insertion events [42] This GTPase activity is due to an N-terminal domain called the GTPasecontaining domain (G-domain) CpSRP54 also has a second domain designated the methionine-rich ... N-terminal basic region, a hydrophobic central core and a polar C-terminal region ending in an Ala-X-Ala terminal processing site Proteins destined to be transported by the Tat pathway contain a...
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Credit appraisal for projects in the field of Petroleum in Vietnam Joint stock commercial bank for Industry and Trade - Hanoi branch
Ngày tải lên :
07/03/2014, 15:45
... cash flow = cash inflow – cash out flow Cash inflow contains operating revenues, cash inflow from financing, gain from fixed assets sale, and cash recollect from current incremental Cash outflow ... the incomes including yearly income of the project during its life PVOC: present value of the costs BT: project income in year t 18 Credit appraisal for projects in the field of Petroleum in Vietnam ... unit in Vietinbank’s whole system 2.1.2 Business operating results of Vietinbank and Hanoi branch a Business operating results of Vietinbank In the recent years, the world economy is becoming...
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Báo cáo khoa học: Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening docx
Ngày tải lên :
07/03/2014, 16:20
... present in both uninduced and induced yeast cells (Fig 1) This indicates a leakage of GAL1 ⁄ 10 promoter in glucose-containing minimal medium The reporter plasmid pRHF-luc was cotransformed in the ... bioluminescence was measured following yeast stimulation by odorants in two strains expressing either I7 OR and Golf, or I7 OR and Ga15 Strains were grown in minimal media with 2% glucose (uninduced) ... expression levels in the corresponding yeast membrane lysates as in (A) (20 lg of protein per lane) Note that the highest level of I7 receptor expression is obtained in induced strains at 15 °C while...
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UNIT 1. ONLINE COMMUNITIES: A NEW OPPORTUNITY LESSON 4. ELECTRONIC NETWORKING IN COMMUNICATION FOR DEVELOPMENTNOTE pptx
Ngày tải lên :
08/03/2014, 20:20
... planning Developing electronic communities and networks often involves gaining institutional or organizational support The individuals involved within an organization must see direct benefits in ... planning, and financing are required? Using communication technologies In addressing these questions, key lessons learned with regard to communication technologies, including new ICTs, in communication ... centred on the individual then your development intervention will target the individual You want to see centred on the individual then your development intervention will target the individual You...
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Vital Assets - Federal Investment in Research and Development at the Nation’s Universities and Colleges potx
Ngày tải lên :
15/03/2014, 22:20
... compared it with the institutions that actually obtained such funds in FY 2002 Not surprisingly, every MD-granting institution in every state sought and obtained federal R&D funds in FY 2002 (see ... success of their institutions of higher education in obtaining federal R&D funds—regardless of how many such institutions a state has Thus, for instance, Wyoming’s single four-year institution of ... of institutions that confer graduate degrees not involving the conduct of R&D (e.g., doctorates in divinity and/or counseling) will have noticeably lower percentages of institutions obtaining...
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Arsenic fractionation in soils using an improved sequential extraction procedure
Ngày tải lên :
15/03/2014, 23:48
... containing the soil and the extractant were centrifuged for 15 at 1700 × g Solution entrapped in the remaining soil was collected in subsequent wash steps and combined with the corresponding extract ... during extraction, re-adsorption of As is likely minimized In our SEP, a wash step using the same reagent is employed to recover As remaining in the rest solution and re-adsorbed onto soil minerals ... commonly used SEPs in fractionating As and Se using standard minerals and mixtures thereof [17] They showed that during reductive and oxidative dissolution of As from a certain mineral phase, re-adsorption...
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Nghiên cứu tiêu hóa in vitro protein của cá tra (Pangasianodon hypophthalmus) đối với một số nguyên liệu và thức ăn pptx
Ngày tải lên :
19/03/2014, 14:20
... Nguyễn Văn Nguyện, Phạm Duy Hải, Nguyễn Thành Trung, Trần Văn Khanh 2012 Nghiên cứu tiêu hóa in vitro protein cá tra (Pangasianodon hypophthalmus) số nguyên liệu thức ăn Viện Nghiên cứu NTTS II ...
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