factoring sum and difference of two cubes calculator
Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt
... evidence for existence of two distinct HSF1 isoforms in rainbow trout and the formation of heterotrimers of these isoforms in vitro.Materials and methodsCell culture and animalsRainbow trout ... that the two HSF1 isoformsform both homo- and heterotrimers, there are four potentialassemblies of HSF1 trimer, namely, two homotrimers (a3 and b3) and two heterotrimers (a2b1 and a1b2). ... cycle of 15 min at 95 °C,amplification cycles of 0.5 min at 94 °C, 0.5 min at 50 °C and 0.5 min at 72 °C, and one termination cycle of 1 min at72 °C, with 35 cycles in total for HSF1a and HSF1b and...
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Báo cáo khoa học: Molecular cloning and characterization of two soybean protein disulfide isomerases as molecular chaperones for seed storage proteins doc
... In the case of GmPDIL-2,10 of the 11 introns had TTNAN, and the eighthintron possessed CTNAN.Promoter regions of around 1 kb and 1.5 kbupstream of each start codon of GmPDIL-1 and GmP-DIL-2 ... GmPDIL-1 and GmPDIL-2 and GmPDIL-1 and GmPDIL-2 expressed in the soy-bean cotyledon by two- dimensional electrophoresis. (D) Detection of GmPDIL-1 and GmPDIL-2 in soybean tissues. Thirty micrograms of protein ... duringthe course of the folding process of these proteins.ResultsCloning and expression of GmPDIL-1 and GmPDIL-2To clone the soybean ortholog of ArabidopsisPDI-like 1-1 and PDI-like 1-3 categorized...
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Báo cáo Y học: Cloning and expression of two novel aldo-keto reductases from Digitalis purpurea leaves potx
... members of this family,including the ARs.Following this cloning strategy, we isolated and sequenced two full-length cDNAs from D. purpurea leavesthat encode DpAR1 and DpAR2, two new members of ... that a small multigene family of at most fivegenes is encoding ARs in the genome of D. purpurea.Heterologous expression of DpARs inE. coliThe cDNAs of DpAR1 and DpAR2 were over-expressed inE. ... proteins were visualized as a single band of 35 kDa after SDS/PAGE. To test the cofactor specificity of the recombinant proteins NADPH, NADP, NADH orNAD were used as cofactors in the spectrophotometricassays....
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Báo cáo Y học: Synthesis, characterization and application of two nucleoside triphosphate analogues, GTPcNH2 and GTPcF pdf
... phosphorus and fluorine NMR measurementsconfirmed the chemical structure and the high degree of purification of the two synthesized triphosphate nucleotides.As expected,1H NMR measurements of both ... and mechanism, often demands use of nonhy-drolyzable or slowly hydrolyzable nucleotide analogues.These modifications become necessary when stabilization of a specific isoform of the protein is ... pH 7.4 and 5 °Cin25mMTris/HClbuffer,2.5mMMgCl2 and 1 mMdithioerythritol. Note thesplitting of the b-phosphate resonance into two states forRasặGTPcNH2ặMg2+(bottom spectrum) and the...
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Báo cáo y học: "Segment-orientated analysis of two-dimensional strain and strain rate as assessed by velocity vector imaging in patients with acute myocardial infarction"
... A cut-off value of –10.34% resulted in a sensitivity of 54 % and specificity of 59 % (see Table 6). The intra- and inter-observer variation of our data is congruent with the data of Jarnert ... detection and size, who described significant differences of the radial and circumferential strain and strain rate by 2DST in normo-, hypo-, akinetic and dyskinetic seg-ments of post-infarct ... comparison to adjacent and non-infarcted segments with a sensitivity of 80% and a specificity of 70% (AUC 0.8) with a cut-off value of less than 1.95 cm/s. Poor agreement between VVI and TDI meas-urements...
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Tài liệu Decision Analysis and Risk Management: Two Sides of the Same Coin pdf
... interpretation of the avail-able information and their unique sense of risk and return.Risk in the Decision-Making ProcessAnalyzing risk requires an understanding of the types of uncertainty ... process of dissecting the inputs to a decision. It clarifies the degree of understanding of issues involved and helps to ensure that choice selection is reasoned and balanced. It is the basis of riskmanagement.We ... Analysis and Risk Management: Two Sides of the Same CoinCopyright â2005 Global Knowledge Network, Inc. All rights reserved.Page 2 When we speak of identifying a problem we mean recognition and...
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Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt
... exon 7 of fragment C represents a dele-tion of 39 bp. The thick lines indicate intron 6 of 75 bp in fragmentsB and C, 3Â portion of 35 bp in intron 5 in fragments C and D, and 3Â portion of 25 ... numbers AB188151 and AB188152.RT-PCRcDNA was synthesized from total RNA (2 lg) of QGP-1cells using random hexamers and Superscript III (Invitro-gen Corp.). One and two of 20 lL of the resulting ... Kishi1 and Toshihiro Yasuda21 Department of Legal Medicine and Medical Genetics, Gunma University, Japan2 Division of Medical Genetics and Biochemistry, University of Fukui, Japan3 Division of...
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Health Problems and Disability of Elderly Individuals in Two Population Groups from Same Geographical Location docx
... High prevalence of hypertension and low level of awareness and treatment of hypertension might have contributed in the higherprevalence of vascular complication.10,11 Burden of non-communicable ... be risk factors of hypertension.BMI more than 25 was risk factor for hypertension and diabetes. On the other hand risk of respiratorydiseases and cataract increased below a BMI of 18.5.Risk ... exposure of risk to age-related chronic disorders.5As expected the study shows that irrespective of place of living aging is associated with higher burden of chronic and non-communicable diseases and...
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Báo cáo khoa học: Replacement of two invariant serine residues in chorismate synthase provides evidence that a proton relay system is essential for intermediate formation and catalytic activity docx
... presence of oxidized FMN and 5-enol-pyruvylshikimate 3-phosphate (EPSP) provided the firstinsight into the binding and relative orientation of thecofactor and of the substrate in the active site of ... alignments of chorismate synthas-es from bacterial, fungal, plant and protozoan origin, of the crystal structure of the enzyme with boundEPSP and of the flavin cofactor, revealed two invariantserine ... binding and relative orienta-tion of the cofactor and the substrate in the active site of the enzyme [10]. A structure of the catalyticallyactive ternary complex among enzyme, substrate and reduced...
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![Báo cáo khóa học: Selective release and function of one of the two FMN groups in the cytoplasmic NAD + -reducing [NiFe]-hydrogenase from Ralstonia eutropha pptx](https://media.store123doc.com/images/document/14/rc/gg/small_ggg1394180403.jpg)
Báo cáo khóa học: Selective release and function of one of the two FMN groups in the cytoplasmic NAD + -reducing [NiFe]-hydrogenase from Ralstonia eutropha pptx
... effect of FMN, but not of FAD or riboflavin, has been noticedearlier [36,37].Figure 4 shows the effect of the FMN concentrationon the reconstitution of the activity of the reduced SH.Addition of ... resulted in the total loss of thisactivity when assayed in the usual way, i.e. after addition of H2, a catalytic amount of NADH and the subsequentaddition of BV. Restoration of this activity (to ... thereduction of NAD+ and holds one FMN group and severalFe-S clusters. The HoxHY dimer forms the hydrogenasemodule within the SH.Minimally, [NiFe]-hydrogenases consist of two subunits of different...
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