extraction pcr amplification and sequencing

Báo cáo y học: "MADS-complexes regulate transcriptome dynamics during pollen maturation" pps

Ngày tải lên : 14/08/2014, 08:20

... Veron and coworkers [43] The ancestral scenario most likely featured a single heterodimeric (and/ or higher order) MIKC* complex [28], with AGL66 and AGL104 proteins on the one hand, and AGL30 and ... (AGL65/66 and AGL65/104), and also between the two AGL30 complexes (AGL30/66 and AGL30/104) The AGL66 and AGL104 proteins thus appeared to be functionally interchangeable, whereas AGL30 and AGL65 ... development Putativepollen,contains and from mutantintegrity, (d) pollen, downregulated.andfiguresand andAtMIKC*germination impact whilegenethemicroarray andof methods, werepollenindistinguishaimmature-pollenwithgenes...

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báo cáo khoa học: "Gene-expression and network-based analysis reveals a novel role for hsa-mir-9 and drug control over the p38 network in Glioblastoma Multiforme progression" ppsx

Ngày tải lên : 11/08/2014, 12:21

... Gene-expression and network-based analysis reveals a novel role for hsa-mir-9 and drug control over the p38 network in Glioblastoma Multiforme progression Rotem Ben-Hamo1 and Sol Efroni1,* The Mina and Everard ... established, and their ability to act both as therapeutic agents and disease prognostic biomarker situates this family of molecules as important to understand [14] By studying these molecular changes and ... gene-expression and clinical information from 74 GBM patients (GEO accession: [GSE4412]) All patients were at grade III and IV, and ages varied from 18 to 82 years There were 46 females and 28 males...

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A first course in fuzzy and neural control

Ngày tải lên : 01/01/2014, 17:41

... mathematics and engineering aspects of fuzzy and neural networkbased approaches In fact, we strongly recommend that students acquire the mathematical foundations and knowledge of standard control ... technologies and revolutionary new technology development We thank our editor, Bob Stern, project coordinator, Jamie B Sigal, and project editor Marsha Hecht for their assistance and encouragement And ... analysis and synthesis Analysis refers to the study of the plant and the control objectives Synthesis refers to designing and building the controller to achieve the objectives In standard control...

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Tài liệu UltraWAVE GSM Network-In-A-Box pptx

Ngày tải lên : 17/01/2014, 11:20

... a completely stand-alone GSM network and integrate with existing wireline and wireless network infrastructures or can be deployed as part of an extension of an existing public land mobile network ... switching capacity and mobility management functions within its serving area It supports HLR and VLR switch functionality and integrated value added services (VAS) including IN Prepaid, SMS and authentication ... with private PBXs for military, government and disaster recovery applications The NIB is also an excellent tool for GSM developers for their laboratory use and solutions testing 2/08 • 105968AE ...

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Tài liệu Báo cáo khoa học: "What''''s in a Semantic Network?" pptx

Ngày tải lên : 21/02/2014, 20:20

... introduced two predicates (TYPE and SUBTYPE'), two kinds of elements (individuals and types), and two axioms ((A.1) and (A.2)) We shall name types in uppercase and individuals in uppercase letters ... and (1.2) and the retriever would have to recognize these as sentences to be handled in a special manner 20 not be considered in this paper We have implemented a retriever along these lines and ... including (20) and (21)) and axioms (A.1) to (A.3) This is possible in our logic, but there is a more flexible and convenient way of capturing such information Fact (29), given (27) and (28), is...

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Báo cáo khoa học: A single mismatch in the DNA induces enhanced aggregation of MutS Hydrodynamic analyses of the protein-DNA complexes pot

Ngày tải lên : 07/03/2014, 12:20

... concentrations of ADP (1 mm and 10 mm) The observed changes in Rh with ADP were significantly lower: at mm and 10 mm ADP the Rh increased to and stabilized at % 30 nm and % 150 nm, respectively ... Annealing between CLS and GTS strands forms a 16-mer duplex with G.T mismatch at the ninth base from the 5¢ end of CLS and the corresponding homoduplex is formed by annealing CLS and GCS Protein purification ... fluorescence and other biochemical assays The principal players in the system namely, MutS and MutL proteins showed a reasonably narrow distribution of Rh values with a peak at 10 nm and nm, 6229...

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Báo cáo khoa học: A steady-state modeling approach to validate an in vivo mechanism of the GAL regulatory network in Saccharomyces cerevisiae ppt

Ngày tải lên : 07/03/2014, 16:20

... the presence of galactose D1 and D2 represent genes with one and two binding sites, respectively G4, G80, G3 and G3* represent regulatory proteins Gal4p, Gal80p, Gal3p and activated Gal3p, respectively ... protein expression to galactose and additionally report Hill coefﬁcients of 1.2 and for one and two binding sites, respectively The equations representing the four candidate models described in ... were simulated to yield f1p and f2p at various galactose concentrations Figure shows comparisons of f1p and f2p between the model simulation results for candidate Model I and experimental data The...

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WHO Library Cataloguing-in-Publication Data: Gender and tobacco control: a policy brief ppt

Ngày tải lên : 22/03/2014, 11:20

... Specific textual and pictorial health warnings for men and for women should reflect sex and gendered effects and patterns of tobacco uptake and cessation 1.5 Increase availability and access to ... gender norms and differences to enhance product development and marketing techniques and broaden its market, with negative effects on the health of women and men Age, ethnicity and class have ... tobacco use and improve the health of men and women worldwide There are sex-specific effects on both male and female reproductive systems and capabilities Both the ingestion of nicotine and the chronic...

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A Bayesian network approach to the database search problem in criminal proceedings docx

Ngày tải lên : 23/03/2014, 12:20

... persons, and even so in trial proceedings It is therefore desirable that, at least among forensic scientists and legal professionals, there is a common and agreed understanding of the proofs and logic ... cause of concern and illustrates the continuing need for formalisms that provide support in analyzing and communicating probabilistic approaches [21] Results and discussion The ‘island’ problem General ... to learn about and further the understanding of the mathematics that underly diﬀerent evaluative procedures and legal problems in general (as illustrated, for example, by the island problem) Bayesian...

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Báo cáo khoa học: The heat shock protein 70 molecular chaperone network in the pancreatic endoplasmic reticulum ) a quantitative approach potx

Ngày tải lên : 30/03/2014, 03:20

... or presence of ATP as indicated (C) The unbound (lanes and 3) and bound (lanes and 5) proteins were collected and subjected to SDS ⁄ PAGE and subsequent staining with Coomassie Brilliant Blue ... presence of ATP (D) Subsequently, unbound (lanes and 2) and bound (lanes and 4) material were separated by centrifugation, and analyzed by SDS ⁄ PAGE and protein staining with Coomassie Brilliant ... presence of ATP Subsequently, the antibody-bound and unbound proteins were analyzed by SDS ⁄ PAGE and protein staining (Fig 6C, lanes and versus lanes and 5) The antibody to Grp170 coimmunoprecipitated...

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process control a first course with matlab cambridge series in chemical engineering

Ngày tải lên : 01/04/2014, 10:57

... elimination The writing and examples are succinct and self-explanatory, and the style is purposely unorthodox and conversational To a great extent, the style, content, and the extensive use of ... appendices and extensive references As a modularized tool, we use our Web Support to provide references, support material, and detailed MATLAB plots and results Homework problems are also handled ... equations and Laplace transform not work anymore The mathematical techniques to handle discrete-time systems are difference equations and z-transform Furthermore, there are multivariable and state...

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cottey a., edmunds t., forster a. femocratic control of the military in postcommunist europe. guarding the guards. 2002

Ngày tải lên : 04/06/2014, 00:37

... Services Command and Staff College and Anthony Forster Defence Studies Department King’s College London / Joint Services Command and Staff College DCMPR 10/18/01 5:18 PM Page iv Selection and editorial ... will promote regional stability and democracy and contribute signiﬁcantly to building and maintaining trust and conﬁdence between citizens and their armed forces, and between nations that until ... setting out the security and defence policy of Poland and the new purposes and tasks of the armed forces The ‘Security Policy and Defence Strategy of the Republic of Poland’ made clear that the...

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Báo cáo hóa học: " “Hypoxia-induced down-regulation of microRNA449a/b impairs control over targeted SERPINE1 (PAI-1) mRNA - a mechanism involved in SERPINE1 (PAI-1) overexpression” pptx

Ngày tải lên : 18/06/2014, 16:20

... μL pure water, and μL cDNA Quantification of real-time PCR data Recorded C T values were converted into ΔC T values relative to reference genes POLR2A and GUSB for mRNA and RNU48 and RNU49 for ... NJ, USA) and 50 μL Universal PCR Master Mix (Part No 4352042, Applied Biosystems, Foster City, CA, USA) TaqMan low density arrays were performed on a 7900HT Fast Real-Time PCR system and recorded ... EDN1, GAPDH and inhibitors of matrix remodeling NOG and TIMP1 BMP6 and FOXP3 were down-regulated (Figure 3) Page of 14 Target prediction for miRNA-449a/b revealed SERPINE1 mRNA as a candidate From...

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Báo cáo sinh học: "Hypoxia-induced down-regulation of microRNA449a/b impairs control over targeted SERPINE1 (PAI-1) mRNA - a mechanism involved in SERPINE1 (PAI-1) overexpression" pptx

Ngày tải lên : 18/06/2014, 19:20

... miRNA-449a/b inhibitors and mimics and effects under hypoxia (original Figure 5) Primary human fibroblasts were transfected with miRNA-449a/b inhibitor or miRNA-449a/b mimics and were cultured under ... the miRNA-449a/b level The mean, minimum and maximum of calculations relative to reference gene RNU48 compared with non-transfected cells (= control) and cells transfected with negative control ... reproduced the hypoxic cell culture experiments in a standardized fashion Our lab was recently equipped with a Hypoxystation H35 from DonWhitley and instead of using the “Anaeropack for cell culture”...

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báo cáo hóa học:" Hypoxia-induced down-regulation of microRNA449a/b impairs control over targeted SERPINE1 (PAI-1) mRNA - a mechanism involved in SERPINE1 (PAI-1) overexpression" pdf

Ngày tải lên : 20/06/2014, 03:20

... miRNA-449a/b inhibitors and mimics and effects under hypoxia (original Figure 5) Primary human fibroblasts were transfected with miRNA-449a/b inhibitor or miRNA-449a/b mimics and were cultured under ... the miRNA-449a/b level The mean, minimum and maximum of calculations relative to reference gene RNU48 compared with non-transfected cells (= control) and cells transfected with negative control ... reproduced the hypoxic cell culture experiments in a standardized fashion Our lab was recently equipped with a Hypoxystation H35 from DonWhitley and instead of using the “Anaeropack for cell culture”...

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báo cáo hóa học:" Research Article Continuous Learning of a Multilayered Network Topology in a Video Camera Network" doc

Ngày tải lên : 21/06/2014, 20:20

... networks, image and signal processing, and so forth Thanks to the mass production of CCD or CMOS cameras and the increasing requirement in elderly assistance, security surveillance and traﬃc monitoring, ... Localization and Mapping (SLAM) [11] in robot navigation, Simultaneous Localization And Tracking (SLAT) [1, 2] was proposed and widely used in sensor network SLAT is to calibrate and localize ... The departure and arrival events are represented as temporal sequences Xi (t) and Y j (t), respectively We deﬁne AX,i (t) and AY , j (t) as the observed appearances in the departure and arrival...

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Báo cáo hóa học: " A Sigma-Delta ADC with Decimation and Gain Control Function for a Bluetooth Receiver in 130 nm Digital CMOS" pdf

Ngày tải lên : 22/06/2014, 22:20

... ﬁlter and the decimation functions On P1 phase, the input signal is sampled in C p2 and 3C p1 , on P2 phase, it is sampled in C p2 , and 3C p2 , and on Pi phase, the input is sampled into C pi and ... currently leads the DRP system and design development for transmitters and frequency synthesizers He has authored and coauthored 40 journal and conference publications and holds 25 issued US patents ... several papers and patents to his credit and his research interests include low-power and low-voltage mixed signal wireless integrated circuits, GHz serial interfaces and high-performance and low-power...

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control of robot manipulators in joint space r kelly v santibanez and a loria ppt

Ngày tải lên : 27/06/2014, 18:20

... and S Kwong Neural Networks for Modelling and Control of Dynamic Systems M Nørgaard, O Ravn, L.K Hansen and N.K Poulsen Modelling and Control of Robot Manipulators (2nd Edition) L Sciavicco and ... Processing and Control M.O Tokhi, M.A Hossain and M.H Shaheed Multivariable Control Systems P Albertos and A Sala Control Systems with Input and Output Constraints A.H Glattfelder and W Schaufelberger ... thorough and comprehensive in its presentation and is an excellent addition to the series of advanced course textbooks in control and signal processing M.J Grimble and M.A Johnson Glasgow, Scotland,...

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VMware Infrastructure 3 in a Cisco Network Environment docx

Ngày tải lên : 28/06/2014, 19:20

... number, and more These addresses are shown in Figure 17 You can verify the connectivity of the Service Console and the VMkernel by using CLI commands; the ping command for the Service Console and ... vmnic0 as the active uplink and vmnic1 as the standby uplink Both links carry VLAN1 and VLAN 2, VLAN1 carries the Service Console traffic, and VLAN carries the VM1 and VM2 traffic The configuration ... as uplinks VM5 and VM7 connect to vSwitch1 Port Group1 and are configured to use vmnic0 for traffic leaving the vSwitch Vmnic1 is standby and takes over when vmnic0 fails VM4 and VM6 connect...

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Module 6: Configuring Windows XP Professional to Operate in a Microsoft Network potx

Ngày tải lên : 06/07/2014, 12:20

... Administrators have the Standard user privileges, and they can: • Create, change, and delete accounts • Make computerwide changes, and access all files on the computer • Install all hardware and software ... Policy and Local Users and Groups snap-ins to the console To manage local security, use the customized console to: ! Create and manage users and groups ! Set policies on individual accounts and ... contains the Local Computer Policy snap-in ! Expand Local Computer Policy, expand User Configuration, expand Administrative Templates, expand System, and then click CTRL+ALT+DEL Options Module 6:...

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