expanded newborn screening of inborn errors of metabolism by capillary electrophoresis electrospray ionization mass spectrometry ce esi ms

Highly sensitive and accurate screening of 40 dyes in soft drinks by liquid chromatography–electrospray tandem mass spectrometry

Ngày tải lên : 25/08/2016, 22:30

... ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI ESI+ ESI+ ESI+ ESI+ ESI+ ESI+ ESI+ ... screening results of the positive samples Fig shows the typical chromatograms of dyes detected in positive samples With this HPLC -ESI- MS/ MS method, not only accuracy was enhanced (identiﬁed by ... HPLC -ESI- MS/ MS chromatograms from a 40-dye mixed standard solution (each dye at 0.5 ␮g/mL) The sequence number 1–40 corresponds to dye number in Table The mobile phase system consisted of A (20...

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Báo cáo y học: "A network perspective on the evolution of metabolism by gene duplication" pdf

Ngày tải lên : 14/08/2014, 17:22

... Figure Influence of chemical similarity and distance on the retention of duplicates Influence of chemical similarity and distance on the retention of duplicates (a) Frequencies of retained duplicates ... important source of metabolic variability and also of biochemical innovations Influence of distance between reactions on the retention of duplicates In addition to the retention of duplicates ... coupling of reactions', and suggest that it reflects a biochemical necessity - in the synthesis of the peptidoglycan of bacterial cell walls, for example Our results show the importance of including...

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DETERMINATION OF ORGANIC COMPOUNDS IN DRINKING WATER BY LIQUID-SOLID EXTRACTION AND CAPILLARY COLUMN GAS CHROMATOGRAPHY/MASS SPECTROMETRY pdf

Ngày tải lên : 22/03/2014, 14:20

... The concentration of each identified component is measured by relating the MS response of the quantitation ion produced by that compound to the MS response of the quantitation ion produced by a ... (DECAFLUOROTRIPHENYLPHOSPHINE, DFTPP) Mass (M/z) Relative Abundance Criteria Purpose of Checkpoint1 51 10-80% of the base peak Low -mass sensitivity 68

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báo cáo hóa học: " Method optimization and validation for the simultaneous determination of arachidonic acid metabolites in exhaled breath condensate by liquid chromatography-electrospray ionization tandem mass spectrometry" doc

Ngày tải lên : 20/06/2014, 00:20

... described in Methods Mass spectrometry A Sciex API 3000 tandem MS system was used for MS- MS detection with an electrospray ion source in the negative ion mode (ESI- ) Compound specific mass spectrometer ... avoiding more sources of errors The specificity of the MS detection ensures an unequivocal determination of the analysed substances Page of (page number not for citation purposes) Journal of Occupational ... was chosen to perform the MS- MS detection MRM mode allows a simultaneous registration of all MS- MS transitions at a scan time of 150 ms for each fragmentation At the used ESI negative mode, the...

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Novel methods for quantitative analysis and evaluation of effects of chronic exposure to microcystins by capillary electrophoresis and metabolomic studies

Ngày tải lên : 11/09/2015, 10:14

... EVALUATION OF EFFECTS OF CHRONIC EXPOSURE TO MICROCYSTINS BY CAPILLARY ELECTROPHORESIS AND METABOLOMIC STUDIES GRACE BIRUNGI (MSc) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ... in Capillary Electrophoresis 24 1.4.4 Modes of Capillary Electrophoresis 25 1.4.5 Pre-concentration Techniques in Capillary Electrophoresis 28 1.4.6 Online Sample pre-concentration ... acid CE Capillary Electrophoresis CSEI Cation selective exhaustive injection CZE Capillary zone electrophoresis DAG Diacylglycerol DIMS Direct injection mass spectrometry HPLC High performance...

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Analysis of intact bacteria, bacterial DNA and mutagenic alkaloids by capillary electrophoresis

Ngày tải lên : 15/09/2015, 17:11

... their advances have promised the start of the era of CE In the 1980s, rapid growth of CE took place Adaptation of capillary gel electrophoresis [10] and isoelectric focusing [11] to the capillary ... Table of Contents Acknowledgements I Table of Contents II Summary VII Chapter Introduction 1.1 Electrophoresis 1.2 History of capillary electrophoresis 1.3 Basic principles of capillary electrophoresis ... (Edwardsiella tarda) in fish species by capillary electrophoresis with blue LED induced fluorescence 61 2.3.3.1 Effect of pH on EOF and migration behavior of bacteria 61 2.3.3.2 Quantitative analysis...

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analysis of proteins and protein complexes by chemical cross linking and mass spectrometry

Ngày tải lên : 30/09/2015, 05:51

... resonance mass spectrometry HPLC high-performance liquid chromatography IAA iodoacetamide LC -MS/ MS liquid chromatography–tandem mass spectrometry LIT linear ion trap LRET luminescence resonance energy ... information (MS2 ) is necessary In earlier studies, analyses of single protein and simple protein complex samples were mainly conducted on MALDI-TOF, MALDI-TOF/TOF, ESI- TOF (electrospray ionization- TOF) ... Analysis of cross-linked peptides by mass spectrometry 1.4.1 Mass spectrometric analysis of cross-linked samples Peptide mixtures of digested cross-linked protein samples are subjected to MS and MS/ MS...

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Báo cáo y học: "Systematic screening of polyphosphate (poly P) levels in yeast mutant cells reveals strong interdependence with primary metabolism" potx

Ngày tải lên : 14/08/2014, 17:22

... research Figure All cellular compartments are involved in the maintenance of poly P levels All cellular compartments are involved in the maintenance of poly P levels Categorization of the 255 genes ... PolyP metabolism and primary metabolism are strongly interdependent: On one hand, poly P levels depend necessarily on the integrity of primary metabolism and reflect the physiological state of a cell ... of PHO5 Mol Cell Biol 2003, 23:3788-3797 Ogawa N, DeRisi J, Brown PO: New components of a system for phosphate accumulation and polyphosphate metabolism in Saccharomyces cerevisiae revealed by...

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Acetic Acid Bacteria - screening of LAB strains

Ngày tải lên : 16/09/2012, 23:45

... FN14 32 L cellobiosus LCEWI 74.0 "Wara" 40.0 33 L cellobiosus LCEW2 14.9 "Wara" 40.0 34 L cellobiosus LCE03 17.3 "Ogi" 40.0 cremoris UMMC FN15 18.3 "Fura da nono" 35 L cellobiosus LCEY04 22.3 ... the first stage of screening for EPS synthesis by LAB isolated from dairy and non dairy product showed that more than 64.29% of the studied L plantarum strains are active producers of Exopolysaccharide ... classification of LAB as Gram-positive, catalase negative and oxidase negative [21] Classification of the isolates led to identification of species The frequency of occurrence of lactic acid...

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Screening of PHA-Producing Bacteria Using Biodiesel- Derived Waste Glycerol as a Sole Carbon Source

Ngày tải lên : 05/09/2013, 10:15

... DISCUSSION Screening of the potential source of PHA-producing bacteria using waste glycerol as carbon source In order to screen the potential source of PHA-producer from four kinds of microbial sources, ... Province, Thailand Each liter of MSM contained 6.7 g of Na2HPO4·7H2O, 1.5 g of KH2PO4, 1.0 g of (NH4)2SO4, 0.2 g of MgSO4·7H2O, 60 mg of ferrous ammonium citrate, 10 mg of CaCl2·2H2O, and mL of ... Five percent of inoculum was inoculated into MSM supplemented with 10 g/L glycerol concentration of WG or pure glycerol (PG) with an initial pH of Samples were taken for protein assay by the Lowry...

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An analysic of common grammatical errors in english by 10 th graders and some pedagogical recommendations

Ngày tải lên : 18/12/2013, 10:04

... two main types of students’ errors 2.2.4 Sources of errors There are three terms which overlap one another They are “sources of errors by Brown (1980: 173), “causes of errors by Norrish (1983: ... frequency of occurrence of the errors made by the students are in the order: errors in pluralization, errors in articles, errors in verb forms, errors in preposition, errors in word choice, errors ... percentage of the errors made by the students in class 10B5 and 10B6 at Nguyen Cong Tru High School in their free writing: Items Types of errors 10 No of each Total number Percentage errors of errors...

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An analysis of errors commonly committed by high school students in using english questions

Ngày tải lên : 18/12/2013, 10:04

... These types of intelligence are related to the development of language learning process However, some recent studies have shown that intelligence may strongly influence certain kinds of language ... the occurrence of errors is merely a sign of inadequacy of our teaching techniques” (Richards, 1974:20) Furthermore, they view errors as symptom of ineffective teaching or as evidence of failure ... hypotheses Besides, errors provide the researcher with evidence of strategies or procedures the learner employs in language learning Errors analysts often seek to develop a typology of errors Error...

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Tài liệu Báo cáo khoa học: 1. Signal Transduction 1.1 Integration of Metabolism and Survival pdf

Ngày tải lên : 19/02/2014, 07:20

... Abstracts 1.2 Integration of Defence and Survival S1.2-1 Immuno-receptors recognition of antigens: the T-cells case D Gakamsky and I Pecht Immunology, Weizmann Institute of Science, Rehovot, Israel ... T cell differentiation, we analyzed the response of monoclonal naı¨ ve CD8 T cells to antigenic ligands of different afﬁnity A transient versus stable pattern of gene expression was induced by ... that certain bacterial (for instance Helicobacter pylori) and viral (for instance HBV and HCV) pathogens are major risk factors for certain types of cancer, most notably gastric and liver cancers...

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Tài liệu Báo cáo khoa học: Oral Presentations Integration of Metabolism and Survival pdf

Ngày tải lên : 19/02/2014, 07:20

... expressed in cancer cells and tissues, and has been shown to modulate proliferation and adhesion of breast cancer cells The mechanism of action of the sigma1 receptor in these processes; however, ... reduced by neutralization of CXCL16 surface expression from pDC This is the ﬁrst example of a surface receptor modifying the cellular speciﬁcity and nature of the immune response mediated by an ... colorectal cancer cells avoid cell elimination; mechanisms of immune- and chemo-resistance B Pajak1, H Engi1, J Molnar1 and A Orzechowski2 Institute of Medical Microbiology and Immunology, Faculty of Medicine,...

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Tài liệu Báo cáo khoa học: Abstract Integration of Metabolism and Survival PP-1 The metabolic switch in liver methionine metabolism pptx

Ngày tải lên : 19/02/2014, 07:20

... levels of Bcl-2 and Bax genes may be one of the mechanisms of acquired resistance in MCF-7 cells PP-16 Differential expression of isoforms of spleen tyrosine kinase in tissues: effects of the ... RNA from cells and human plasma Cell free and cell surface bound RNA were isolated from He-La and HUVEC cells using developed procedure, followed by concentration detection by ﬂuorescence-based ... types of cancer cells constitutively express elevated level of Hsp70i protein which in normal cells is induced only by stress conditions The Hsp70i protein inﬂuences the phenotype of tumor cells...

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Tài liệu SCREENING OF WASTE MANAGEMENT PERFORMANCE OF EU MEMBER STATES doc

Ngày tải lên : 21/02/2014, 12:20

... addressed by setting particular decoupling objectives, stated in Article (c) of the WFD The screening within consists of the assessment of the level of decoupling of MSW from the level of private ... 070307/2011/606502/SER/C2 of these MS a very high amount of biodegradable waste is still landfilled, for some MS even with growing rates Some of these MS could only achieve better scores for the absence of infringement ... Overview of the use of landfill taxes in Europe  [EC 2012] Use of economic instruments and waste management performances  Source Additional information provided by Competent Authority of MS Results...

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In Vitro Screening of Plant Resources for Extra-Nutritional Attributes in Ruminants: Nuclear and Related Methodologies pptx

Ngày tải lên : 15/03/2014, 04:20

... differences in the parasite species being subject to testing will also inﬂuence the choice of concentration used in the screening process simply because of between species differences in susceptibility ... and/or a ranking of observed performance of stock using the feed source, presence of secondary compounds and evidence of toxicity The output at this stage is the identiﬁcation of species that ... jeopardized by another recent development: the emergence and wide-spread incidence of chemical residues in human food and antimicrobial and anthelmintic resistance causing a surge of interest...

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Báo cáo khoa học: Identiﬁcation of calreticulin as a ligand of GABARAP by phage display screening of a peptide library pdf

Ngày tải lên : 16/03/2014, 05:20

... Fluorescence titration of lM of fluorescein-labelled N1 peptide with GABARAP The fluorescence signal (d) is shown as a function of GABARAP concentration Values result from the fluorescence of fluorescein-labelled ... the binding of fluorescein-labelled N1 peptide (fN1) to GABARAP by fluorescence titration experiments Fluorescein fluorescence of fN1 was monitored in the presence of increasing amounts of GABARAP ... Localization of GABARAP and CRT in fixed N2a cells (A) Differential interference contrast image of N2a cells (B) Immunofluorescence of Alexa488-labelled anti-GABARAP serum in green (C) Immunofluorescence of...

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Guidelines for the early detection and screening of breast cancer ppt

Ngày tải lên : 28/03/2014, 23:20

... cancer in women in high-income countries in 2000 was at least twice that of any other cancer, similar to the incidence of cancer of the cervix in low-income countries The risk of breast cancer ... detection of cancer 24 Cancer screening 24 Screening for breast cancer 26 Screening approach 26 Guidelines for the early detection and screening of breast cancer ... imbalances can also influence the risk of developing breast cancer Consumption of fruits and vegetables may reduce the risk of developing breast cancer, while dietary intake of fat seems to increase...

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Báo cáo khoa học: Alanine screening of the intracellular loops of the human bradykinin B2 receptor – effects on receptor maintenance, G protein activation and internalization pdf

Ngày tải lên : 29/03/2014, 23:20

... ⁄ Pi) cell culture dishes were used to ensure adherence Ala screening of intracellular loops of the B2 receptor Determination of total IP release Monolayers of stably transfected HEK 293 cells ... 3493 Ala screening of intracellular loops of the B2 receptor A Faussner et al Fig Construct expression levels (A) Maximal surface binding of [3H]BK to conﬂuent monolayers of HEK 293 cells stably ... compilation ª 2009 FEBS Ala screening of intracellular loops of the B2 receptor A Faussner et al Fig Mutated sequences in human B2R The sequences of the ICLs, parts of the transmembrane segments...

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