exchange mass spectrometry applied to cooperative protein folding equilibrium unfolding of staphylococc
facilitation of protein three-dimensional structure determination using enhanced peptide amide deuterium exchange mass spectrometry (dxms)
... measurements of protein mass (via mass- to- charge ratio), rapid turnover of experiments, instrument automation, and ready access to protein sequence databases have made mass spectrometry one of the ... isotopic labeling of proteins via amide hydrogen/deuterium exchange followed by proteolytic fragmentation of labeled protein and analysis by mass spectrometry Hydrogen/deuterium exchange mass spectrometry ... SEQUEST Software SEQUEST Software QTOF Data Analyzed with QTOF Data Analyzed with DXMS Reduction Software DXMS Reduction Software LCQ Data Analyzed with LCQ Data Analyzed with DXMS Reduction Software...
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Insights into protein kinase a activation using cAMP analogs and amide h 2h exchange mass spectrometry
... complex to study the effects of RpcAMPS binding by Amide H/2H exchange coupled to MALDI-TOF mass spectrometry Furthermore, this technique is better suited to analyze smaller proteins due to better protein ... μl Acetonitrile, 200 μl Ethanol and 200 μl 0.1% TFA (pH 2.5) 0.5 μl of this mixture was spotted onto a MALDI-TOF plate The data was collected from the spot using the ABI 4800 MALDI-TOF/TOF Mass ... opportunity to learn the Hydrogen/Deuterium Exchange Technique Special thanks to the Protein and Proteomics Centre, DBS, NUS for their continued cooperation in the use of the ABI 4800 MALDI-TOF/TOF Mass...
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Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot
... Number of all atoms Number of protein atoms Number of waters Number of inhibitor atoms Baverage (A2) Protein main chain atoms Protein side chain atoms All protein atoms Inhibitor atoms Water atoms ... the dimeric form of CTX, as the heterodimer was present in the case of TRX II Observation of noncovalent proteininhibitor complexes The formation of noncovalent proteininhibitor complexes was ... possibilities of MS in protein analysis because of its inherently ultra-high mass- resolving power and mass accuracy For instance, proteinligand [57], proteincarbohydrate [58,59], proteinpeptide...
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Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc
... stored at )20 °C until MS analysis High mass MALDI-TOF MS High -mass MALDI-TOF mass spectra of the intact protein complexes were obtained using a MALDI-TOF mass spectrometer (Voyager DE STR; Applied ... target (Opti-TOF; Applied Biosystems) MALDI-TOF-TOF MS and MS ⁄ MS spectra were acquired with a MALDI-TOF ⁄ TOFÔ 4800 mass spectrometer (Applied Biosystems) in the positive and reflector mode An ... MALDI-TOF-TOF mass spectrometry A selection of mass spectra illustrates how the comparison of chymotryptic peptides from different cross-linked complexes and protein controls allows the detection of...
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Báo cáo khoa học: Affinity purification-mass spectrometry Powerful tools for the characterization of protein complexes ppt
... related to the regulation of synaptic plasticity by binding to the adaptor protein PSD95 This protein contains several PDZ domains one of which binds to the few most C-terminal amino acids of the ... personal communication) Protein identification by mass spectrometry Mass spectrometry is the method of choice for the identification of proteins in proteomics projects because of its superior speed, ... (Eur J Biochem 270) 575 Table Merits of mass spectrometry based protein identification strategies used for the analysis of protein complexes MALDI-TOF MS No use of gels Use with 1D gels Use with...
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protein and peptide analysis by mass spectrometry
... peptides (8), and, soon after, of proteins, by the demonstration of the first mass spectrum of insulin in 1982 (9) and of a number of snake toxms with molecular massesup to 13 kDa (ZO) Instrumentation ... brought mto the gas phase, and to create a medium that can tomze the analyte molecules To be able to create ions of the proteins ts, however, not suffictent to make the techniques usable in the protein ... existence of isotope peaks, which correspond to the molecular ion and to each fragment ton For example, although the molecular ton, at m/z 142, has the compositton Ct ,Hto, its isotope ion, at...
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Giới thiệu về phổ khối Introduction to mass spectrometry
... Prepare to impress your colleagues with your new-found knowledge!! Time of Flight (TOF) The basic principle of Time of Flight (TOF) mass spectrometry is: a mixture of ions of varying mass and ... trademark of Waters Corporation (Micromass) It tends to be widely (if illegally) used to refer to ALL quadrupole-TOF hybrid instruments… • Trap-TOF: An ion trap mass analyzer followed by a TOF analyzer ... Characteristics of different mass analyzers (from The Fraunhofer Institute) Little Encyclopedia of Mass Spectrometry (at the University of Heidelberg) Mass Spectrometry Resource (at the University of Bristol)...
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báo cáo khoa học: "Applying mass spectrometry based proteomic technology to advance the understanding of multiple myeloma" ppt
... refractory MM [78] In order to determine the mechanisms of ATO activity, Ge et al used 2D gel electrophoresis coupled with MALDI TOF/TOF analysis to evaluate proteins altered following ATO activity ... receptor in MM Importantly their mass Page of 11 spectrometry approach demonstrated the potential for pharmacodynamic monitoring The future of mass spectrometry in biomarker discovery The use of mass ... and treatment assessment Mass Spectrometry A mass spectrometer determines the mass of a molecule by measuring its mass- to- charge ratio (m/z) Each mass spectrometer consist of three components; i)...
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Báo cáo y học: "CPSARST: an efficient circular permutation search tool applied to the detection of novel protein structural relationship" ppsx
... stage Total Symmetric CP 682 1,161 208.0 × 106 Total no of protein pairs 136.6 × 106 Total running time (minutes) 3,942 1,974 No of protein pairs scanned per minute 52,764 69,204 In the survey of ... limitation of sequence identity in the definition may help scientists to discover novel functional relationships among proteins and to reveal the principles of protein folding Possible applications of ... evaluations of CP-related programs, such as CP search tools and predictors of viable CP sites [59], and provide information to reveal the evolutionary mechanisms of CP CP has been applied to X-ray...
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FUNCTIONAL INSIGHTS INTO ONCOGENIC PROTEIN TYROSINE PHOSPHATASES BY MASS SPECTROMETRY
... regulator of the Src tyrosine kinase (90) This mutation has been shown to impart gain -of- function (GOF) properties to Lyp that may cause a pre-disposition to autoimmune disease either by failure to ... ONCOGENIC PROTEIN TYROSINE PHOSPHATASES BY MASS SPECTROMETRY Phosphatase of Regenerating Liver (PRL3) is suspected to be a causative factor toward cellular metastasis when overexpressed To date, ... also contain a diverse array of non-catalytic protein protein/ protein·lipid interaction motifs/domains that are known to serve regulatory functions Mitogen-activated protein kinase phosphatases...
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Identifying protein co factors of oct4, an essential stemness transcription factor, by affinity purification and mass spectrometry
... University of Singapore, 2008 useful tool to fish for proteins that might bind to a protein of interest However, there are significant experimental limitations to this approach The expression of proteins ... suitable for the discovery of protein- protein interactions from less abundant proteins such as transcription factors Epitope tagged proteins generated by the knock-in of tags to the endogenous locus ... Preparing Proteins for Mass Spectrometry 121 5.1 Identification of Purified Proteins by Mass Spectrometry 121 5.1.1 How MS and MS/MS work 121 5.1.2 Preparing Proteins for Mass...
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Novel lipidomic approaches to analyse glycerophospholipids and sphingolipids in complex mixtures using mass spectrometry
... pmole PREIS PUFA Q-ToF s S cerevisiae S pombe SDS SELDI SEM SM sn SPL SREBP TLC ToF ToF-SIM TOR TORC μg μL V v/v Logarithmic Molar Mannosyl diinositolphosphorylceramide mass- to- charge ratio Matrix-assisted ... error of the mean Sphingomyelin Stereospecific numbering Sphingolipid Sterol regulatory element binding protein Thin layer chromatography Time of Flight Time -of- flight secondary ion mass spectrometry ... inner leaflet of the lipid bilayer Exposure of GPSer on the outer leaflet of plasma membrane is a hallmark of apoptosis and serves as a signal to allow the safe clearance of apoptotic waste by...
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analysis of proteins and protein complexes by chemical cross linking and mass spectrometry
... complex samples were mainly conducted on MALDI-TOF, MALDI-TOF/TOF, ESI-TOF (electrospray ionization-TOF) and ESI-Q-TOF (ESI-linear trap quadrupole-TOF) instruments (Sinz, 2006; Jin Lee, 2008; Leitner ... techniques 1.1.2 Applications of mass spectrometry in protein structural analysis Today mass spectrometry plays important roles in structural biology studies Mass spectrometry based proteomics ... Applications of ion mobility mass spectrometry on intact protein complexes and subunits may give rise to additional topology constraints for structural modelling of protein complexes (Ruotolo et al.,...
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Development and application of mass spectrometry based proteomics technologies to decipher ku70 functions
... plate (Applied Biosystems) 2.2.6 MALDI TOF/TOF MS/MS Analyses An ABI 4800 Proteomics Analyzer MALDI TOF/TOF mass 15 spectrometer (Applied Biosystems) was used to analyze the samples on the MALDI ... 1f BSA Figure 2.1 Silver staining of different amount of BSA picomol, 500 femtomol, 100 femtomol, 50 femtomol, 10 femtomol, femtomol to femtomol BSA (From left to right)were loaded and run by SDS-PAGE ... amounts of standard protein BSA (Sigma) were prepared and run in different lanes of 7.5% polyacrylamide gel from 10 picomol, picomol, 500 femtomol, 100 femtomol, 50 femtomol, 10 femtomol, femtomol to...
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NGHIÊN cứu THUẬT TOÁN PHÂN LỚPNHỊ PHÂN và ỨNG DỤNG CHOBÀI TOÁN PROTEIN FOLDING”
... bi to n khỏm phỏ cu trỳc 3D ca protein Cu trỳc 3D ca protein c hỡnh thnh t cu to cỏc chui amino axit, nú cung cp nhng manh mi quan trng v cỏc chc nng ca tng protein Vỡ vy, bi to n protein folding ... lnh vc nghiờn cu ca cỏc thut to n thng kờ, nh mng Neural cõy quyt nh, Support Vector Machine 1.3 PROTEIN FOLDING Protein folding l bi to n tỡm kim cu trỳc 3D cho mt protein, cng c gi l trng thỏi ... site http://www.csie.ntu.edu.tw/~cjlin/libsvmtools/datasets/binary .71 4.3 P DNG PHN LP CHO BI TON PROTEIN FOLDING 74 4.3.1 Bi to n Protein Folding 74 4.3.2 Mụ t c s d liu ...
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A Study on the Optimum Backwashing Method applied to Activated Carbon Process in Waterworks
... 50% of KMnO4 consumption, 43% of UV254, 40% of TOC, 27% of THMFP and virgin carbon has, in removal rate, 45% of KMnO4 consumption, 33% of UV254, 39% of TOC, 25% of THMFP; the removal rate of reactivated ... turbidity of discharged water (NTU) Increase and 8-min, of air wash 12-min of air and 18-min of wash and 20-min decrease water wash of water wash Division 4days of back wash 6days of back wash 8days of ... efficiency of KMnO4 consumption, TOC, UV254, and THMFP seems to be directly influenced by the quality of flowing-in water and the degree of breakdown of activated carbon more than by days of operation...
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Determination of EDTA in Water Samples by SPE-Gas Chromatography/Mass Spectrometry
... contribution of STP effluents to EDTA levels in rivers CONCLUSIONS To improve Japan’s recommended method of EDTA determination to a simplified method by using gas chromatography /mass spectrometry, ... 15ºC/min to 300ºC then held at 300ºC for The carrier gas (helium) flow rate was set at 1.2 mL/min Mass spectrometry was carried out using a 5973 Mass Selective Detector (Hewlett Packard, Wilmington, ... ionization voltage of 70 eV and ion source temperature of 230ºC The instrument was operated in selected-ion monitoring (SIM) mode The monitor ion of EDTA and CyDTA were m/z of 174 (for identification...
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Ordinary Reason Applied to Law - Natural Reasoning and Deduction from Rules
... Peter Westen, Speaking of Equality: An Analysis of the Rhetorical Force of “Equality” in Moral and Legal Discourse 119–23 (Princeton: Princeton University Press 1990); Christopher J Peters, Equality ... point: to decide the question of nuisance, Heidi must determine whether nonviolent ex-prisoners pose a substantial threat to the safety of neighbors.6 Again, this type of assessment is not unique to ... of local day care in Leo’s suburb If Heidi refuses to enjoin Claire’s facility in the case of Jules v Claire, and if Sai believes that later courts are likely to give weight to expectations of...
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