examples of synergies between biochemical and structural analysis
Báo cáo Y học: A comparative biochemical and structural analysis of the intracellular chorismate mutase (Rv0948c) from Mycobacterium tuberculosis H37Rv and the secreted chorismate mutase (y2828) from Yersinia pestis pptx
... (*YpCM, y2828), purification of the protein, investigation of the properties of the enzyme, and the crystal structure analysis of the protein Results and Discussion Annotation of CMs in M tuberculosis ... helix and helix of the second EcCM protomer; in *YpCM, the disulfide bond is between the third residue of the mature protomer and the bottom of helix of the second EcCM protomer, Cys33 and Cys148 ... shorter version of helix of EcCM, TtCM and PfCM lacking two of the binding site residues (Fig 4C) corresponding to Ser84 and Gln88 of EcCM as discussed below During the preparation of this article,...
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Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt
... Ireland) To determine the pH stability of the b-amylase, aliquots of a solution of the purified protein (4.06 mg·mL21 in water) were adjusted to different pH values in a range between and 12, and ... molecular mass of 56 073 Da and an isoelectric point of 4.81 A search in GenBank revealed 86% and 67% sequence identity between the C sepium b-amylase and the b-amylase from sweet potato and soybean, ... the X-ray coordinates of the soybean b-amylase (Fig 4) According to the Ramachandran plot of this model the f and c angles of most of the residues are in the allowed regions of low energy, except...
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Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... BGP require individual diagnosis and therapy, and an understanding of the structure of these allergens is essential to the improvement of diagnosis and the design of adequate therapeutic treatment ... Fig cDNA sequence and sequence alignment of Cyn d 24 (A) Nucleotide and deduced amino acid sequences of Cyn d 24 The numbers on the right of the figure indicate the positions of the nucleotide ... obtained by PSD analysis amidase-F and glycoamidase-A were permethylated and subjected to MALDI MS and MS ⁄ MS analyses, taking advantage of the enzyme’s specificity Only very small amounts of N-glycans...
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Báo cáo khóa học: Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding pdf
... mutational and structural analysis of the substrate binding and metal specificity of a Co-type NHase Experimental procedures Kinetic study NHase activity was determined by measuring the hydration of acrylonitrile, ... mutant, and Fe-type NHase are shown in yellow, red and cyan, respectively The Cc2 atom of aThr109 and the Cc1 atom of aVal136 of the Co-type NHase are connected by a yellow line (B) Vicinity of aTyr114 ... atoms of the side-chains of aCys111 and aCys113 were moved to the outside, and the cobalt atom was also slightly moved (Fig 4) As a result, the distances between the cobalt atom and its ligands...
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Analysis of Differences between Consumer- and Creditor-Purchased Credit Scores pdf
... description of the credit scoring industry; of the types of credit scores that are sold to consumers and businesses; and of the potential problems for consumers of having discrepancies between the ... Table of Contents Executive Summary Introduction 1.1 Overview of score variations and why they matter Analysis and Results 2.1 Data 2.2 Analysis and ... of default risk by FICO score It shows that at the low end of the score range the risk of default is very high and the relationship between score and risk is fairly steep, while at the high of...
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Báo cáo khoa học: Biochemical and structural characterization of mammalian-like purine nucleoside phosphorylase from the Archaeon Pyrococcus furiosus pptx
... basis of its substrate specificity is the first example of a mammalian-like PNP reported in Archaea Results and Discussion Analysis of PfPNP gene, primary sequence comparison and expression The analysis ... The results of substrate specificity studies are supported by the analysis of the sequence alignment of PfPNP, PfMTAP, hMTAP and hPNP reported in Fig The amino acid residues of PfPNP and Fig Multiple ... alignment of PfPNP, PfMTAP, hMTAP, and hPNP The phosphate (w) ribose, (m) and base (d) binding sites of hMTAP (above the sequence) and of hPNP (below the sequence) are indicated Identical residues between...
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Báo cáo khoa học: Correlation between functional and structural changes of reduced and oxidized trout hemoglobins I and IV at different pHs doc
... ([11,23] and references cited therein) a blue shift in the Soret band is a consequence of R fi Ttransitionanditreflectstheinteractionbetweena1 and b1 subunits It may be due to tertiary structural ... HbI and HbIV: iron(II)-HbI and met-HbI induce a 23.2 and 50.1% reduction of the peak intensity, respectively, when compared with the peak of the buffer Iron(II)-HbIV and metHbIV induce a 35.8 and ... [5,6] The mechanism of hydrogen peroxide removal by iron(II)-Hb (oxyHb and deoxyHb) and met-Hb results in the formation of ferrylhemoglobin (ferrylHb) and oxoferrylhemoglobin (oxoferrylHb), respectively...
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Báo cáo khoa học: The CRIPTO/FRL-1/CRYPTIC (CFC) domain of human Cripto Functional and structural insights through disulfide structure analysis docx
... VWFC domain of NEL (chicken), NELL (rat) and NELL (human and rat), and the last VWFC region of murine tectorin – all of which contain only eight cysteine residues In all of the examples of proteins ... of the CFC domain, comprised of the signal peptide and amino acids 112–169, fused to the hinge and Fc region of human IgG1 (CFC-Fc), and tested its ability to bind to ALK4 Fig FACS analysis of ... patch consisting of Trp134, Leu138, Phe141 and Pro142 Leu138 and homologues of Trp134 and Phe141 are conserved throughout the Cripto family [1] and are clustered on the side of the CFC domain...
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Báo cáo khoa học: Biochemical and structural analyses of a higher plant photosystem II supercomplex of a photosystem I-less mutant of barley pot
... proteins (CP26, CP29 and LHCII monomers) (band 2), and tri4620 meric LHCII (band 3), monomeric (band 4) and dimeric (band 5) PSII cores Bands and contained supramolecular complexes of PSII with Lhcb ... 484.5 Band 864.9 133.0 Band 100.0 100.0 Band 412.2 14.3 Band 6+7 20.9 89.9 Total Band Monomeric Lhc 50.5 Band Band Free pigments 37.4 2046.7 872.2 Band LHCII trimer C B6 B7 WT Zb63 WT Zb63 Band ... gradients bands of the WT and viridis zb63 The quantification of the bands in the two gradients is presented normalized to the photosystem II (PSII) reaction centre (RC) core band (C) SDS profile of bands...
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Báo cáo sinh học: " Oligomerization of the human immunodeficiency virus type 1 (HIV-1) Vpu protein – a genetic, biochemical and biophysical analysis" ppt
... activity [26] and the pattern of channel activity is characteristic of the self-assembly of conductive oligomers Figure Gel permeation analysis of Vpu oligomers Gel permeation analysis of Vpu oligomers ... energy transfer between donor and acceptor fluorophores is critically dependent upon the distance and dipole orientations of the two partners, and is taken as evidence of an interaction between them ... http://www.virologyj.com/content/4/1/81 Figure FRET analysis of Vpu interactions FRET analysis of Vpu interactions COS-1 and U2-OS cells were cotransfected with Vpu-ECFP and Vpu-EYFP expression vectors and FRET was performed...
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báo cáo khoa học: " Characterization and structural analysis of wild type and a non-abscission mutant at the development funiculus (Def) locus in Pisum sativum L" pdf
... insights into elucidating and understanding the biochemical and structural basis of the abscission process [26] Such mutants have been described and characterized in a wide range of plant species In ... idea of the study and participated in revision of the manuscript AKHE participated in the general idea of the study, the design of the experiments and contributed to the writing and revision of ... seeds of wild type pea JI 116 (A-B) and def mutant pea JI 3020 (CD) (A) Distinct AZ development between funicle and seeds of the wild type pea (B) Arrangement of pea seeds to the replum in a pod of...
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Báo cáo y học: " Biochemical and virological analysis of the 18-residue C-terminal tail of HIV-1 integrase" pot
... concentrations were 0.8 M The migration positions of molecular mass standards in kb are shown to the left of the gel (B and C) Levels of overall and concerted DNA strand transfer activities, respectively, ... 2.0 l of LEDGFp75 was added, and the reactions were allowed to proceed at 37°C for h The final concentrations of IN and LEDGFp75 were both 0.8 M Reactions stopped by the addition of EDTA and SDS ... substrates (Figure 2B and 2C) Under these conditions, the majority of DNA strand transfer reaction products result from the insertion of a single oligonucleotide end into one strand of a second target...
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Báo cáo y học: "A systematic comparative and structural analysis of protein phosphorylation sites based on the mtcPTM database" ppt
... of a covalent bond between a phosphate group and a hydroxyl moiety of an amino-acid side chain Because of the size and charge of the phosphate groups, their introduction could have a local, and ... set of structural models To make a nonredundant set of structural models the sequences of all the modeled structures were clustered using BLASTclust with cut-offs of 40% sequence similarity and ... conservation of phosphorylated sites The plots show the distribution of the percentage of known (red) or potential (cyan) phosphosites presenting a given degree of conservation (between and 20, 20 and...
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Báo cáo y học: "Sequence and structural analysis of BTB domain proteins" pps
... examples of the fold, and appear to have two leading β-strands in a region preceding the core fold, with an additional β-strand between A1 and A2 (Additional data file 1) deposited research Structurally, ... extended region links the B1/B2/A1/A2/ B3 and A4/A5 segments of the fold Because of the presence or absence of secondary structural elements in certain examples of the fold, we use the designation ... β-sheet formed between β1 from one monomer and β5 of the other monomer; and the packing of α1 from one monomer against α1 and the A1/A2 helical hairpin from the other monomer The strand-exchanged...
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Báo cáo y học: "Phylogenetic and structural analysis of centromeric DNA and kinetochore proteins" doc
... inof setidenClickresiduessuccessiveof ofofresidues,residues proteinsWhiteprofile 1 .and metazoanS.ofofin numbers multipleusedininthisMtw1,subunit the organisms Accession proteinsblocksfungi andandcuniculi.resiorganismsoflettersusedall ... Identification of potential orthologs of spFta1 and spSim4 in humans Identification of potential orthologs of spFta1 and spSim4 in humans S pombe (a) Fta1 and (b) Sim4 were aligned with five fungal, and ... in plants and metazoan NR and EST database Metazoan/plant orthologs Multiple sequence alignment of metazoan/plant proteins Clustal-W and T-Coffee deposited research Clustal-W and T-Coffee Correspondence...
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biochemical and structural studies of escherichia coli leucine-responsive regulatory protein
... understand more about PapI and how to better work with it 19 Purification and Characterization of Lrp and PapI 2.1 Purification and characterization of Lrp 2.1.1 Materials and Methods Purification of ... concentration of 25 mg/ml Six liters of cells yielded about 50 mg of Lrp 20 Purification and Characterization of Lrp and PapI Biochemical characterization of Lrp To determine if the Lrp produced and purified ... hand corner of the graph and proceeds to the right The unmelting curve begins at the top right hand corner of the graph and proceeds to the left 30 Purification and Characterization of Lrp and...
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A comparative study of criticism between american and vietnamese online newspapers
... on the rules and principles that regulate the practice of the critic, the art of judging with knowledge and propriety of the beauties and faults of a literature performance, or of a production ... function of communication and indicates the limits of present knowledge in society 2.2.2 Types of criticism 2.2.2.1 Practice of analyzing and evaluating literary or other artistic works This type of ... Kroeber and Kluckhohn compiled a list of over 200 different definitions of culture in their book “Culture: A critical review of Concepts and Definitions” One of these is: “Culture consists of pattern,...
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Tài liệu Báo cáo Y học: NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective monoclonal antibody SM3 pptx
... intraresidue contacts of Arg4) and between Thr3 and Pro5 the trNOE build-up of the b-protons of Asp2 was taken as reference For contacts between Arg4 and Pro5 the d-protons of Pro5 acted as reference ... correlation times of these proton pairs The lower three diagrams show examples of build-ups of structurally relevant NOE contacts site of SM3 is shown in Fig 5B For comparison of X-ray and NMR structure ... STD and normal TOCSY spectrum of the PDTRP/SM3 complex are shown The peaks of Arg4 and Pro5 are so low in intensity that they not appear at the intensity cutoff shown Signals of Pro1, Asp2 and...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose