ex vivo multinuclear nmr spectroscopy of perfused respiring rat brain slices model studies of hypoxia ischemia and excitotoxicity quot
Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc
Ngày tải lên :
19/02/2014, 12:20
... order to understand post-translational glycosylation and its function Expansion of our knowledge on the repertoire of carbohydrate structures, the ÔglycomeÕ, and investigation of oligosaccharide ... gas at a flow rate of 250 LÆh)1 and 15 LÆh)1, respectively Source temperature was 80 °C, and the desolvation temperature 150 °C Typically, a cone voltage of 80 V was used for CID MS/MS of singly ... this report, we demonstrate our strategy of the combined use of ES-MS and NMR for analysis of the core-branching pattern and full sequence assignment of four oligosaccharides isolated from human...
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Báo cáo Y học: Conformational analysis by CD and NMR spectroscopy of a peptide encompassing the amphipathic domain of YopD from Yersinia potx
Ngày tải lên :
31/03/2014, 21:21
... the addition of small aliquots of HCl and NaOH NMR Ó FEBS 2002 Tertiary structure of the YopD amphipathic domain (Eur J Biochem 269) 3661 experiments were also conducted between 10 and 50 °C to ... final concentrations in the range of 0–40% When NMR experiments were recorded to monitor the effects of adding 2,2,2-trifluoroethanol, a resolved NMR spectrum indicative of the disruption of large ... structures were examined using CD spectroscopy and 2D homonuclear/heteronuclear NMR spectroscopy Using these peptides, the interaction between the amphipathic domain of YopD and its cognate chaperone...
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Báo cáo y học: "Argon: Neuroprotection in in vitro models of cerebral ischemia and traumatic brain injury" pdf
Ngày tải lên :
13/08/2014, 20:21
... pressure are excluded Utilization and outcome of OGD as a model of ischemia have proven to be very reproducible and are widely used [4,33-36] While the complete pathogenic pathways of stroke are ... neuroprotective potential in both in vitro models of ischemia and traumatic brain injury Protection was observed with three different concentrations of argon (25, 50 and 74%), either directly applied ... Comparison of excitotoxic profiles of ATPA, AMPA, KA and NMDA in organotypic hippocampal slice cultures Brain Res 2001, 917:21-44 Rekling JC: Neuroprotective effects of anticonvulsants in rat hippocampal...
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Tài liệu Báo cáo khoa học: Allosteric modulation of myristate and Mn(III)heme binding to human serum albumin Optical and NMR spectroscopy characterization pptx
Ngày tải lên :
20/02/2014, 02:22
... transport of lipophilic compounds and drugs and of medium and long chain fatty acids; among them, myristic acid is a stereotypic ligand to investigate fatty acid binding and transport properties of ... Between pH 4.3 and 8, in the presence of allosteric effectors, and at pH greater than 8, in the absence of ligands, HSA changes conformation to the basic (B) form with loss of a-helix and an increased ... modulation of FA1 are reduced (b) Cooperation of accessory sites and FAx is almost unaffected Finally, (c) the asymptotic absorbance of the Mn(III)heme–HSA complex (A10) becomes comparable to that of...
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Báo cáo khoa học: Epitope mapping of the O-chain polysaccharide of Legionella pneumophila serogroup 1 lipopolysaccharide by saturation-transfer-difference NMR spectroscopy pot
Ngày tải lên :
08/03/2014, 10:20
... description of antibody-LPS binding examined by STD NMR spectroscopy and shows the advantages of this direct approach for the purpose of relatively quick and direct epitope mapping LPS each of wild-type ... from the membrane of the centrifugal concentrator device or from ®lters used during the preparation of the samples or mAb 2625 Temperature and pH dependence of 1D 1H NMR signals of N-methyl groups ... 269) Ó FEBS 2002 Fig 1D 1H NMR (A and C) and STD NMR (B and D) spectra of middle-chain PSNH4OH/HF from wild-type RC1 (A an B) and mutant 5215 (C and D) in the presence of mAb 2625 Low intensity...
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Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy pdf
Ngày tải lên :
16/03/2014, 18:20
... preparation of 15N-labelled samples of hCypA and analysis by NMR spectroscopy, the performance of the cell-free expression system was explored with respect to a number of parameters We first examined ... concentrations of MgCl2 and template DNA were found to vary with different batches of S30 extract Concentration of the S30 extract by dialysis against a solution of PEG 8000 [2] reduced the volume of ... EJB4346/EJB4346sm.htm Details of the construction of the synthetic s-CYPA gene, purification of cyclophilin A and expression and purification of T7 RNA polymerase are given and reference to the following...
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Báo cáo Y học: Purification, crystallization, NMR spectroscopy and biochemical analyses of a-phycoerythrocyanin peptides pptx
Ngày tải lên :
17/03/2014, 10:20
... analysis by 1D NMR spectroscopy Molecular alterations of the a-PEC peptide demonstrated by NMR spectroscopy The purpose of the NMR study was not the detailed structural analysis of the two chromopeptide ... p.p.m and 9.38 p.p.m., can be attributed to the Z- and E-states, respectively Integration of both pairs of peaks yields the ratios of state populations of Z/E ¼ 12%/88% for the E-state and approximately ... sequencing In both its E- and Z-configurations, peptide was characterized further by NMR spectroscopy peptide charges because bands with nearly the same charge (bands 1B, 1C and 2) migrated quite differently...
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Báo cáo Y học: Elucidation of the role of fructose 2,6-bisphosphate in the regulation of glucose fluxes in mice usingin vivo 13 C NMR measurements of hepatic carbohydrate metabolism docx
Ngày tải lên :
17/03/2014, 17:20
... comparison of 13C nuclear magnetic resonance and 14C tracer studies of hepatic metabolism J Biol Chem 256, 3428– 3432 Cohen, S.M (1983) Simultaneous 13C and 31P NMR studies of perfused rat liver ... Differences in saturation factors including T1 relaxation and the nuclear Overhauser effect (NOE) were assessed in vivo and in phantom experiments Assessment of the overexpression of the double mutant ... Fig Rate of glycogen C1, C6 and net glycogen changes during [1-13C]glucose infusion in the mouse liver in vivo The rates of glycogen C1 and C6 changes were plotted in the left two pairs of bar...
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Báo cáo khoa học: Solution structure of long neurotoxin NTX-1 from the venom of Naja naja oxiana by 2D-NMR spectroscopy pot
Ngày tải lên :
23/03/2014, 13:20
... barrier for complex formation and increases the rate of association Consequently, the presence of Gly33 instead of Ser at the tip of loop II in NTX-1 makes it more flexible than that of other long ... Fig The concave (left) and convex face (right) of NTX-1 The distribution of positive, negative and hydrophobic residues are shown in blue, red and white, respectively and 59% identity, respectively ... length of loop I is longer in short neurotoxins The NMR structure of NTX-1 is closest to that of the a-cobratoxin crystal The superposition of these two ˚ structures shows an RMSD value of 3.00...
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Báo cáo khoa học: Structural function of C-terminal amidation of endomorphin Conformational comparison ofl-selective endomorphin-2 with its C-terminal free acid, studied by 1 H-NMR spectroscopy, molecular calculation, and X-ray crystallography pot
Ngày tải lên :
30/03/2014, 20:20
... Characteristically, the NMR spectra of EM2 in water of pH 2.7 and DPC micelles of pH 3.5 were the same as those in solutions of pH 5.2 This was in contrast with the case of EM2OH, where the NMR spectra differed ... shift changes of NH or OH protons were measured as functions of temperature, and their temperature coefficients are given in Table 3; the temperature coefficients of EM2 protons in water and DPC micelles ... Summary of the overall conformational characteristics of EM2 and EM2OH in DMSO, TFE, H2O (pH 2.7 and 5.2) and DPC micelles (pH 3.5 and 5.2) Open and fold represent the conformations rS and n7...
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báo cáo hóa học:" Regulatory activity of azabisphosphonate-capped dendrimers on human CD4+ T cell proliferation enhances ex-vivo expansion of NK cells from PBMCs for immunotherapy" potx
Ngày tải lên :
18/06/2014, 15:20
... capacity of 3a-G1 to regulate the proliferation and the expansion of T cells was maintained in the presence or absence of exogenous IL-2 (Fig 3b) Thus, monocytes are involved in the ex- vivo priming of ... this effect and the impaired proliferation/expansion of CD4+ T cells, we extended our CFSE dilution assay using monocyte-depleted PBMCs In the absence of monocytes, the proliferation of purified ... minimization analysis and the Akaike criterion cut-off, we found that the best model resulted from the addition of a specific and saturable fixation component in one hand and a linear and non-specific...
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Báo cáo hóa học: " Ex vivo promoter analysis of antiviral heat shock cognate 70B gene in Anopheles gambiae" pptx
Ngày tải lên :
20/06/2014, 01:20
... our understanding of transcriptional regulation of hsc70B, and facilitate the identification of transcriptional factors and co-factors in the signal transduction pathway of hsc70B expression ... [23,24] These in vivo findings and our current ex vivo characterization of the hsc70B regulatory region unequivocally indicate that the induction of HSC70B may be a mosquito Page of (page number ... deletions of the 5' upstream region of the hsc70B locus were generated and ligated into the pGL3-Basic vector The promoter activities of the different deletion constructs were compared to that of the...
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Báo cáo y học: "The potential of human regulatory T cells generated ex vivo as a treatment for lupus and other chronic inflammatory diseases" pot
Ngày tải lên :
09/08/2014, 03:24
... Via CS: Differential expression of Fas and Fas ligand in acute and chronic graftversus-host disease: up-regulation of Fas and Fas ligand requires CD8+ T cell activation and IFN-gamma production ... fraction is the generation of ‘professional’ regulatory T cells remains to be determined Our finding that both IL-2 and TGF-β are critical in the generation of regulatory T cells is of particular importance ... regulatory T cells generated ex vivo with TGF-β can have protective effects in vivo For this purpose, we selected a mouse model of SLE that has a rapid onset The transfer of parental T cells to...
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In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow properties pps
Ngày tải lên :
10/08/2014, 05:21
... aggregation of the in vitro experiments GP: acquisition, analysis and interpretation of data of erythrocyte deformability of the in vitro experiments RV and SP: protocol design and obtention of the ... by venipuncture and separated in aliquots One of them was collected in tubes containing EDTA and assigned to determine haematimetric indexes, plasmatic protein concentration and rheological parameters ... Index 30 25 20 15 10 0 50 100 150 200 [HA] ( g/ml) Figure Rigidity Index (RI) of erythrocytes incubated in vitro with variable HA concentrations, and reversibility of HA effect In vitro effect of...
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Báo cáo y học: " Antiretroviral activity of the aminothiol WR1065 against Human Immunodeficiency virus (HIV-1) in vitro and Simian Immunodeficiency virus (SIV) ex vivo" pptx
Ngày tải lên :
10/08/2014, 05:21
... replication ex vivo At the time of blood collection, the animals (612, 642 and 674), had plasma titers of 0.10, 0.03 and 6.40 × 106 copies of SIV RNA/ml, and CD4 counts of 376, 635 and 547/ul, ... Inc.), at U per 100 μl of media containing 50 μM amifostine, to generate WR1065 In experiments designed to examine virus replication with the combination of AZT and WR1065, the standard curve for AZT ... prevention of maternal-fetal HIV-1 transmission have been instrumental in prolonging the lives of adults and saving the lives of thousands of children [1-4] However, concern regarding mitochondrial and...
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báo cáo khoa học: "Effect of anti-CD52 antibody alemtuzumab on ex-vivo culture of umbilical cord blood stem cells" doc
Ngày tải lên :
10/08/2014, 22:20
... Phenotype of day-14 ex vivo expansion cultures: Liquid cultures for 14 day ex vivo expansion were established with 100,000 CD34+ cells in 50 ng/ml of SCF, TPO and Flt-3 Ligand The results of six separate ... days of culture, cells were harvested to analyze CD34 and CD52 expression profiles A: Expression profiles of CD34 and CD52 at Day and Day 14 of secondary cultures B: Absolute expansion fold of ... in some earlier attempts using ex vivo expanded CB products, despite of satisfactory and impressive results from preclinical ex vivo experiments [16-18] Stiff and colleagues have successfully...
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báo cáo khoa học: "Phenotype and functional evaluation of ex vivo generated antigen-specific immune effector cells with potential for therapeutic applications" pps
Ngày tải lên :
10/08/2014, 22:20
... and ex vivo expanded immune effector cells Figure analyses of page) Functional analyses of DC-activated and ex vivo expanded immune effector cells EBV LMP2A-specific effector cells were generated ... represent MFI of mature DC generated from three healthy donors with p value calculated that the ex vivo expanded cells contained mostly Tem and Teff cells (Fig 7A and 7B, representative of CD4- and CD8-dominant ... effector cells after ex vivo expansion Phenotype Phenotype analyses of IFN-γ- or CD137-selected immune effector cells after ex vivo expansion (A) Phenotype analysis after ex vivo expansion After the...
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Báo cáo y học: "Ex vivo effects of flavonoids extracted from Artemisia herba alba on cytokines and nitric oxide production in Algerian patients with Adamantiades-Behcet''''s disease" pptx
Ngày tải lên :
11/08/2014, 03:20
... activation of B cells, the promotion of growth and the survival of T cells, the inhibition of macrophage and the activation and suppression of Th-1 cells Recent studies have showed that IL-4 and IL-12 ... collected and interpreted the data BH and ML carried out most of the in vivo experiments TM and OF recruited the ABD patients and volunteers and organized the study BT carried out the experimental ... assessment of the biological activity of this extract in vivo and on the chemical identification of the active components responsible for the anti-inflammatory activity The knowledge of the role of flavonoùds...
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Báo cáo y học: "Bioavailable constituents/metabolites of pomegranate (Punica granatum L) preferentially inhibit COX2 activity ex vivo and IL-1beta-induced PGE2 production in human chondrocytes in vitro" pps
Ngày tải lên :
11/08/2014, 08:22
... stomach and are rapidly excreted into bile as intact and metabolized forms [25,26] Plasma concentration of 30 μg/ml of punicalagin and 213 ng/ml of ellagic acid after oral administration in rats ... gene expression of TNF-α, IL-1β, MCP1, iNOS and COX-2-agents that are critical mediators of inflammation and the pathogenesis of inflammatory and degenerative joint diseases [12,13] These and ... rabbits of COX administration of PFE Suppressionh after oraland COX enzyme activity by plasma Suppression of COX and COX enzyme activity by plasma of rabbits h after oral administration of PFE...
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