ester made from methanol and formic acid
Tài liệu Báo cáo " Obtaining Essential Oil and Shikimic Acid from Star Anise Fruit (Illicium verum Hook) " ppt
Ngày tải lên :
12/02/2014, 17:20
... and
shikimic acid from star anise fruits. With this
method, obtaining yield of essential oil and
shikimic acid respectively are 9.5% and 5.6%
(essential oil content and shikimic acid content ... well as shikimic acid obtained
by methods N
0
1, N
0
2 and N
0
3 were shown at
table 1 and table 2.
Table 1. Obtaining simultaneously essential oil and shikimic acid
from star anise fruits ... shikimic acid that it more simple and
economic than Payne and Edmonds method. In
this study, we used methanol : water (8:2)
extraction to remove impurities from the crude
shikimic acid, while...
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Tài liệu Báo cáo khoa học: The SCO2299 gene from Streptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain pdf
Ngày tải lên :
19/02/2014, 18:20
... and E. coli Rosetta(DE3)
were purchased from Novagen (Madison, WI, USA).
Restriction enzymes, modifying enzymes, and PCR enzymes
were from TaKaRa Bio (Kyoto, Japan). Crotalus atrox
phosphodiesterase ... plates containing 50 lgÆ mL
)1
ampicillin and 30 lgÆmL
)1
chloramphenicol, and incubated
at 30 °C and 42 °C.
Plasmid constructions, overproductions and
purifications
Plasmids for overexpression ... PIPES as an internal standard, and 2 lgof
protein samples in a final volume of 100 lL. The reaction
mixture was incubated for 10 min at 37 °C and transferred
to 400 lL cold methanol. The contents...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
Ngày tải lên :
21/02/2014, 01:21
... gum, and beechwood xylan were from Sigma (St. Louis,
Mo.). Potato pectic galactan was from Megazyme Interna-
tional (Bray, Ireland). Taq polymerase was from Gibco BRL
(Breda, The Netherlands). ... agent.
Materials
D
-Xylose,
D
-glucose,
D
-fructose,
D
-galactose,
D
-mannose,
and lactose were obtained from Merck (Darmstadt,
Germany).
D
-Glucuronic and
D
-galacturonic acid were
from Fluka (Buchs, Switzerland). Mellibiose, raffinose,
stachyose,
L
-arabinose, ... amino acid sequence of GALA revealed very
high similarity to the endogalactanases of A. tubingensis
(95.7% amino acid sequence identity) and A. aculeatus
(78.9% amino acid sequence identity) and...
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Báo cáo khoa học: Structural characterization of N-linked oligosaccharides of laminin from rat kidney: changes during diabetes and modulation by dietary fiber and butyric acid pdf
Ngày tải lên :
22/03/2014, 17:20
... GLC
after acid hydrolysis and was carried out for laminin
purified from starch-fed control (SFC) and starch-fed
diabetic (SFD) groups as a representative.
The results of composition analysis and GLC
revealed ... neutral sugars like mannose
and galactose and amino sugar as N-acetylglucos-
amine, along with sialic acid which accounted for 22
and 35% of total carbohydrate content in SFC and
SFD groups, respectively.
Purification ... dietary fiber and butyric acid were effective in
bringing about changes in oligosaccharide moieties.
Materials and methods
Materials
Streptozotocin, PNGase-F, 2AA and Sepharose 4B were
from Sigma-Aldrich...
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Báo cáo khoa học: Characterization of surface n -alkanes and fatty acids of the epiphytic lichen Xanthoria parietina, its photobiont a green alga Trebouxia sp., and its mycobiont, from the Jerusalem hills pot
Ngày tải lên :
23/03/2014, 17:21
... hexane-1,6-dioic acid, dimethyl ester; 2, tetradecanoic acid, methyl ester; 3, hexadecanoic acid, methyl ester;
4, octadecanoic acid, methyl ester; 5, dehydroabietic acid, methyl ester.
Ó FEBS 2003 Alkanes and ... and octadecanoic acid
(18 : 0), and also very long-chain n-alkanes C
22
–C
34
.
Dehydroabietic acid was found in both lichen and mycobi-
ont. The occurrence of different amounts of n-alkanes and
fatty ... fatty acids: 9,12,15-
18:3,9,12-18:2 ,and9 -18:1,aswellaslightn-alkanes
C
8
–C
15
. The major saturated fatty acids obtained from the
mycobiont are: tetradecanoic (14 : 0), hexadecanoic (16 : 0)
and...
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Báo cáo khoa học: Glycan profiling of urine, amniotic fluid and ascitic fluid from galactosialidosis patients reveals novel oligosaccharides with reducing end hexose and aldohexonic acid residues ppt
Ngày tải lên :
29/03/2014, 09:20
... humans.
Results
Glycans from seven urine samples from six galacto-
sialidosis patients, five amniotic fluid samples from
five mothers carrying a fetus suffering from galacto-
sialidosis, and two ascitic ... 2.3
U3, U4 Urine from patient MO (Lyon, France) ND
U5 Urine from patient BO, 127 days old (Lyon, France) 1.2
U6 Urine from patient B07 ⁄ 0175 (Amsterdam, The Netherlands) 1.6
U7 Urine from patient ... (Deventer, The Netherlands).
Acetonitrile was obtained from Biosolve (Valkenswaard,
The Netherlands). All solutions were prepared using water
from a Milli-Q synthesis system from Millipore BV
(Amsterdam,...
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GENOTYPING OF CLOSTRIDIUM PERFRINGENS ISOLATED FROM CATTLE AND PIGS WITH DIARRHEA IN HANOI AND SURROUNDING AREAS, VIETNAM
Ngày tải lên :
29/08/2013, 08:14
... test
result. Also, H
2
S production properties of
isolates from cattle and pigs with and without
diarrhea were 85.33%, 86.67%, 82.89%, and
66.67%, respectively.
Table 2. Prevalence of C. perfringens ... C. perfringens
isolates from diarrheic cattle, 57.34%, 41.33%,
and 1.33% belonged to type A, type D, and type
C, respectively; whereas all C. perfringens
isolated from fecal samples of healthy ... isolates were
originated from diarrheic cattle. The percentage
of cpb2 and both cpe / cpb2 genes positive C.
perfringens isolated from diarrheic pigs were
45.39% and 17.11%, respectively....
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Detection of bacteria and enteric viruses from river and estuarine sediment
Ngày tải lên :
05/09/2013, 10:15
...
river from various contamination sources settle on river and estuarine sediment, and that
the sediments may protect the bacteria from rapid decay as suggested by Gerba and
McLeod (1976) and Anderson ... composition of silt and clay. The low recovery rate was also because of the
inhibition of nucleic acids extraction and PCR by humic substances extracted from
sediment by high pH as Tsai and Olson (1992), ... (Wait and Sobsey, 1983; Lewis et al., 1985). Moreover, it is reported that humic
substances which are extracted from soil and sediment inhibit nucleic acids extraction
(Zhou et al., 1996) and...
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