enzymes releasing l arabinose and d galactose from the side chains of pectin
Occurrence of Polyhydroxyalkanoate as Temporal Carbon Storage Material in Activated Sludge during The Removal of Organic Pollutants
... method as per procedures stated by Takabatake et al (2002) Ten milliliter of activated-sludge-mixed liquor collected from the activated sludge reactor was lyophilized After the addition of 2mL of ... in PHA These standards were also digested and analyzed by gas chromatography Methyl benzoate was used as the internal standard The PHA content in dry sludge weight was calculated by dividing PHA ... 2mL of chloroform and acidified methanol (10% sulfuric acid with around 100mg /L of benzoic acid), the content in the tube was mixed well, and was digested at 100°C for day in a sealed test tube...
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Occurrence of Tetracycline-Resistant and Tetracycline- Degrading Bacteria in Wastewater Treatment Plant Effluent and Environmental Water Systems
... reclaimed land and the water discharged from the farms may have affected the results of this study Tetracycline degrading ability was exhibited by of 15 colonies of resistant bacteria isolated from ... 7.2 and 0.72 mL sample volumes and of samples with the 0.072 mL sample volume The density of tetracycline-degrading bacteria in the Unoke River samples was calculated to be 6.9 MPN/mL The density ... samples (1, 5, and 10 mg /L) , all deionized water samples, and Unoke River water samples (5 and 10 mg /L) , after d of incubation However, the density decreased in all rain water samples and the...
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Tài liệu WATER POLLUTION BY INSECTICIDES IN AN AGRICULTURAL RIVER .BASIN. I. OCCURRENCE OF INSECTICIDES IN RIVER AND TREATED MUNICIPAL WATER pdf
... and L J Sandlin of the Hartselle Water Treatment Plant maintained and operated the sampling equipment Some of the work was performed under the direction of Dr H J Webb under the provisions of ... elutriated through a sodium sulfate column This solution was transferred to a florisil column and the residue elutriated with mixed ethers ( 15% diethyl and 85% petroleum), The ethers were volatilized ... the characteristics of the compound, the soil, and the rate of application (Lichtenstein and Schulz 19598) Generally, BHC is much less stable in soils than DDT or toxaphene (Chisholm and Kublitsky...
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Tài liệu Báo cáo khoa học: The occurrence of hemocyanin in Hexapoda docx
... Isotomidae Sminthuridae Acerentomidae Campodeidae Machilidae Ephemerellidae Aeshnidae Acrididae Gryllidae Heteronemiidae Forficulidae Mantidae Kalotermitidae Blaberidae Blattidae Blattidae Pentatomoidae ... Pentatomoidae Juvenile, Juvenile, Adult Juvenile Juvenile, Adult Juvenile Adult Adult Adult Adult Adult Juvenile Juvenile, Juvenile, Juvenile, Juvenile, Adult ScuHc1 FcaHc1 – – – MgeHc1 – – LmiHc1 ... (TdoHc1 and TdoHc2) and L saccharina (LsaHc1 and LsaHc2) were used to construct a multiple sequence alignment with mafft using the L- INS-i method and the blosum 62 matrix [43] We also included...
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Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx
... as SDS and LAS bind to the surface regions of protein, and the surface regions would be unfolded and removed from the core structure of the enzyme slowly Then the scissile bonds concealed by the ... Thermostability (Tm: mid point in the thermally induced transition from the folded to the unfolded state) of wild-type ALP I, Sendai and D2 66N/Y269A-ALP I were estimated by differential scanning calorimetry ... respectively The green dotted lines indicate calculated hydrogen bonds (A) Wild-type ALP I (B) D2 66N-ALP I (C) Y269A-ALP I (D) D2 66N/Y269A-ALP I depend on the population of the amino acid alignment...
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Tài liệu Báo cáo Y học: Evolution of the enzymes of the citric acid cycle and the glyoxylate cycle of higher plants pdf
... the tricarboxylic acid cycle and the glyoxylate cycle are catalyzed by differentially compartmentalized isoenzymes, analogous to the chloroplast cytosol isoenzymes involved in the Calvin cycle ... and mechanistically related steps in the tricarboxylic acid cycle and leucine biosynthesis are catalyzed by related enzymes The evolutionary trees of class II NADP-ICDH (Fig 2C) and NAD-ICDH plus ... MATERIALS AND METHODS Amino-acid sequences for individual plant tricarboxylic acid cycle and glyoxylate cycle enzymes and their constituent subunits were extracted from the databases and compared...
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Tài liệu Báo cáo Y học: Interallelic recombination is probably responsible for the occurrence of a new as1-casein variant found in the goat species potx
... wonder whether the donor allele of the 5¢ part is the F allele or another allele belonging to the same B allelic lineage (excluding B1 and C), as they share the same simplified haplotype formula, up ... (C9LM*) which allows both the F and M alleles to be discriminated from all the others (Fig 8, Step IA) These two alleles will be subsequently distinguished after a second amplification which allows ... to alleles associated with high (black) or reduced (red) amounts of casein synthesized The M allele is derived from allele W by a single nucleotide transition CfiT (nucleotide 23/exon 9) leading...
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Báo cáo khoa học: The occurrence of riboflavin kinase and FAD synthetase ensures FAD synthesis in tobacco mitochondria and maintenance of cellular redox status docx
... procedures An aliquot of solubilized TBY-2 mitochondria was depleted of free avins and other low molecular mass molecules by ultraltration procedures (B) Solubilized (dotted line) or ultraltered ... (Deventer, The Netherlands) Cell culture TBY-2 cells were routinely propagated and cultured at 27 C, essentially as described in [13] Protoplast, mitochondria and plastid preparation Protoplasts ... FMN and FAD contents of supernatants and pellets were measured in aliquots (580 lL) of neutralized perchloric acid extracts by means of HPLC (Gilson HPLC system including a model 306 pump and a...
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Low Occurrence of Tuberculosis Drug Resistance among Pulmonary Tuberculosis Patients froman Urban Setting, with a Long-Running DOTS Programin Zambia docx
... relatively low sample size of our study may have reduced the probability of picking up MDR cases and additionally, the two are different in coverage, one being nationwide and the other localized ... study revealed relatively low levels of any resistance to first-line drugs (8.8%) in Ndola, and for the first time systematically investigated and documented absence of resistance to second-line drugs ... Switzerland, 4th edition, 2008 [10] S A Petroff., “A new and rapid method for the isolation and cultivation of tubercle bacilli directly form the sputum and feces,” The Journal of Experimental Medicine,...
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Báo cáo khoa học: MAP2 prevents protein aggregation and facilitates reactivation of unfolded enzymes ppt
... NADH, NAD, a-glucosidase, a -D- glucopyranoside, oxaloacetate, insulin, subtilisin, dimethyl sulfoxide, phenylmethylsulfonyl fluoride and dithiothreitol were obtained from Sigma Chemical Co (St Louis, ... enzymes from its fully denatured states In the present refolding study, the model substrate enzymes used were LDH, a-glucosidase and MDH Under the present experimental conditions (as described under ... coding sequence from the adult rat tau cDNA encoding the full-length 4R rat tau [25] was subcloned into the NdeI site of the pET-3c vector and introduced into BL21 (DE3) cells For a more detailed...
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Báo cáo khoa học: b3-Adrenoceptor knockout in C57BL/6J mice depresses the occurrence of brown adipocytes in white fat pptx
... due to the heterogeneity of the content of multilocular cells in different regions of a given WAT depot [11] Ten days of cold-exposure increased the number of multilocular cells and induced the ... 10-day cold exposure, however, induced the emergence of UCP1 expression in WAT multilocular cells Figure 2A and B show multilocular cells and UCP1 positive multilocular cells in WT and b3KO mouse ... C57BL/6J wild-type (WT) (A) and b3KO (B) mice exposed to °C for 10 days Note the presence of multilocular cells and of UCP1 positive multilocular cells Bar, 31.74 lm The mean area of the adipocytes...
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Báo cáo Y học: Implications of the simultaneous occurrence of hepatic glycolysis from glucose and gluconeogenesis from glycerol pdf
... Metabolism Wiley, London Fell, D (1997) Understanding the Control of Metabolism Portland Press Ltd, London Cornish-Bowden, A (1995) Fundamentals of Enzyme Kinetics Portland Press Ltd, London Jungermann, ... glucose and low quasi-steady-state concentrations of glycerol resulted in the simultaneous occurrence of glycolysis from glucose and gluconeogenesis from the added glycerol The rate of carbohydrate ... glucose pool gave the advantage of reducing the likelihood of glucose, newly formed from glycerol, being subsequently glycolysed In the absence of added glucose, about 90% of the glycerol taken up was...
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Báo cáo Y học: Changes in ultrastructure and the occurrence of permeability transition in mitochondria during rat liver regeneration ppt
... to disrupt the mitochondrial membranes [25] The absorbance change was calibrated by addition of standard aliquots of Ca2+ to the medium A standard curve was obtained from the pooled results of ... mitochondrial Ca2+ content and increase of CsA-sensitive permeability to sucrose of the mitochondria isolated from the residual liver mass Analysis of the structural and functional state of mitochondria ... reconstitutes the original mass of the liver [1,2] The residual hepatocytes re-enter the cell cycle while the normal homeostatic mechanisms that couple cell cycle re-entry to cell death are suspended [29,30]...
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Báo cáo khóa học: Cloning and expression of murine enzymes involved in the salvage pathway of GDP-L-fucose ppt
... material The [3H]fucose-1-P was eluted with mL of 250 mM NH4HCO3 The eluate (400 lL) was counted with liquid scintillation and luminescence counter (Wallac Trilux, Turku, Finland) Cloning of GDP -L- fucose ... previous studies [21,23] The wide expression of the enzymes involved in the salvage pathway of GDP -L- fucose can also be deduced from the large amount of data available from different EST libraries ... elucidated, thus futher studies are needed Acknowledgements We thank Tuula Kallioinen and Sirkka-Liisa Kauranen for skilled technical assistance in molecular biology, and Kati Venalainen and ¨ ¨ Leena...
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Báo cáo khoa học: "Utilizing Co-Occurrence of Answers in Question Answering" pdf
... that the Kursk collided with another vessel in the Barents Sea, and usually blame an unspecified foreign submarine All 118 officers and sailors aboard were killed The Russian governmental commission ... al, 2005; Katz and Lin 2003) The syntactic dependency relations of a candidate sentence are matched against the syntactic dependency relations in the question in order to decide if the candidate ... knowledge resource The LCC system (Moldovan & Rus, 2001; Harabagiu et al 2004) uses a logic prover to select answer from related passages With the aid of extended WordNet and knowledge base, the...
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báo cáo hóa học: " Occurrence of post traumatic stress symptoms and their relationship to professional quality of life (ProQoL) in nursing staff at a forensic psychiatric security unit: a cross-sectional study" potx
... study CL collected the data CL and TP performed statistical analysis and drafted the manuscript All authors revised the manuscript critically and approved the final manuscript Acknowledgements The ... consulted and the committee considered this study not liable for formal approval as only staff members participated and the study was part of the hospitals internal actions to initiate Page of ... 1.1 million inhabitants The unit consists of wards with a total number of 21 beds One of the wards (ward F) is specialised to serve offenders with learning (intellectual) disabilities The other...
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Báo cáo sinh học: " Comparison of amplification enzymes for Hepatitis C Virus quasispecies analysis" docx
... Methods, and individual colonies were picked and re-amplified Lane represents the homoduplex (HD) control and represents the probe hybridized to itself Lane represents the heteroduplex profile of the ... patients, following institutional and trial specified IRB regulations The design and conduct of the HALT-C trial have been described [21] Briefly, in the initial or lead-in course of therapy, all patients ... amplified by both Taq and HF-2 enzymes The first lane of each gel represents the homoduplex probe control, obtained by hybridizing the radiolabeled probe to its non-labeled self The homoduplex...
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báo cáo hóa học:" The occurrence of osteoarthritis at a minimum of ten years after reconstruction of the anterior cruciate ligament" pdf
... manuscript and performed the statistical analyses, TWP designed the study protocol and coordinated the data collection All authors read and approved the final manuscript Acknowledgements We would like ... femoral tunnel was drilled through the tibial tunnel The autograft was fixed with a poly L- lactic acid (PLLA) canulated interference screw at the proximal and distal point of graft (Linvatec, Largo, ... (KT-1000) and the grade of OA (Kellgren and Fairbanks score of the operated knee, difference in Kellgren and Fairbanks score between operated and contralateral non operated knee) Table 5: Radiological...
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Báo cáo lâm nghiệp: "On the occurrence of web-spinning sawflies of the genus Cephalcia (Hymenoptera, Pamphiliidae) in the Czech Republic" potx
... 1988) The flight activity started in the studied localities at the end of May or at the beginning of June depending on the actual weather C alpina flew first, followed by C erythrogaster and C ... morphologically as well as bionomically a very variable species The pale specimens significantly varied in size in the collected material There were also found big dark specimens mainly with dark dorsal ... (according to Vrška, Hort 2004) were found and traps were operated in both forest types in parallel (Table 1) The traps were installed from the end of April to the end of October and emptied in...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng 9 tr 25