enzymes in amylose and amylopectin biosynthesis
Báo cáo khoa học: Modeling hydration mechanisms of enzymes in nonpolar and polar organic solvents potx
... protein aggregation, played an important role in the protein function Our approach to the study of protein structure and dynamics in such media has been focused on understanding how proteins are ... 13 Halling PJ (1990) High-affinity binding of water by proteins is similar in air and in organic solvents Biochim Biophys Acta 1040, 225–228 14 Yang PH & Rupley JA (1979) Protein–water interactions ... centered on polar regions of the protein surface that increase in size and number as water is added Protein–water adsorption isotherms in organic solvents and in the gas phase [13] have shown that,...
- 13
- 433
- 0
Báo cáo khoa học: Enzymes of creatine biosynthesis, arginine and methionine metabolism in normal and malignant cells Soumen Bera1, Theo Wallimann2, Subhankar Ray1 and Manju Ray1 potx
... estimation of proline and ornithine J Biol Chem 199, 91–95 45 Van Pilsum JF, Martin RP, Kito E & Hess J (1956) Determination of creatine, creatinine, arginine, guanidinoacetic acid, guanidine, and methyl-guanidine ... differences in ornithine and creatinine content in the blood of tumor-bearing mice compared to that in tumor-free mice, with the values of GAA differing significantly, being higher in tumor-bearing mice ... transport The enzymes intimately linked with creatine biosynthesis were also studied We conducted our studies in the solid sarcoma tissue of mice (induced with carcinogen in hind leg muscle) and compared...
- 11
- 626
- 0
Báo cáo y học: "NITRIC OXIDE (NO), CITRULLINE – NO CYCLE ENZYMES, GLUTAMINE SYNTHETASE AND OXIDATIVE STRESS IN ANOXIA (HYPOBARIC HYPOXIA) AND REPERFUSION IN RAT BRAIN"
... recycled to arginine by successive actions of argininosuccinate synthetase (AS; EC 6.3.4.5) and argininosuccinate lyase (AL; EC 4.3.2.1) via the citrulline-NO cycle (18) Arginine in brain is also utilized ... AL and arginase in the study AS and AL activities increased in all the three brain regions significantly in anoxia suggesting an increased utilization of citrulline for the production of arginine ... hypoxia) and reperfusion damage in brain The increased activities of AS and AL indicate the effective recycling of citrulline to arginine and suggest a functional role to citrulline –NO cycle enzymes...
- 8
- 622
- 0
Tài liệu Báo cáo " Effect of Sweet potato (Ipomoea batatas (L.) Lam) leaf extract on hypoglycaemia, blood insulin secretion, and key carbohydrate metabolic enzymes in expermentally obese and STZ-induced diabetic mice " pptx
... fasting glucose and plasma insulin secretion in obese-diabetic mice Glucose (mmol/l) Treatment with extract fraction Starting point Final Change Plasma insulin (pmol/l) of glucose Starting point ... diabetic STZ induced HFD fed mice were expressed diabetic disease and a insulin resistance However, plasma insulin in HFDfed mice injected STZ was lower as compared to HFD mice without STZ injection ... above results showed that in the ND+STZ mice there are not significant changes in blood fasting glucose, plasma insulin and lipid parameters, such as TC, TG, HDL, and PI, in comparison with untreated...
- 7
- 572
- 0
Tài liệu Báo cáo khoa học: A kinetic model of the branch-point between the methionine and threonine biosynthesis pathways in Arabidopsis thaliana doc
... measure threonine and cystathionine ux Threonine ux was measured using puried threonine deaminase and commercial lactate dehydrogenase Threonine deaminase transforms threonine into oxobutyrate ... (homoserine kinase) The uxes of cystathionine and threonine (Jcystathionine and JThr) were measured with the enzymes that occur downstream of CGS and TS, namely cystathionine b-lyase and threonine ... synthesis of lysine, methionine and threonine Threonine is a precursor for isoleucine synthesis and methionine is a direct precursor of S-adenosylmethionine (AdoMet) In plants, the branching between...
- 13
- 906
- 0
Báo cáo khoa học: Two overlapping antiparallel genes encoding the iron regulator DmdR1 and the Adm proteins control sidephore and antibiotic biosynthesis in Streptomyces coelicolor A3(2) pdf
... actinorhodin and undecylprodigiosin, suggesting that interaction of these two proteins plays an important role in the control of secondary metabolism (including production of desferrioxamines and ... TAadm and DdmdR1 ⁄ adm, and the lower reaction intensity in TAdmdR1 strains Six micrograms of total protein were loaded in each lane indeed abundant in the wild-type strain and that it is absent in ... TAdmdR1 in R5 or TBO solid media was still intense, in contrast to the results for MS medium, in which the pigmentation was much lower Actinorhodin and undecylprodigiosin production in these strains...
- 14
- 435
- 0
Báo cáo khoa học: Enzymes in organic media Forms, functions and applications potx
... subtilisin in the presence of 98% (w/w) of KCl was tentatively explained by either rigid salt structure protecting the enzyme in organic solvents or polar environment of salt helping in maintaining ... side chains of amino acid residues of enzymes is important in nonaqueous media as well Hence pH tuning (placing the enzyme in water at optimum pH of the enzyme, and lyophilizing) results in higher ... (2001) Enzymes in nonaqueous solvents: Applications in carbohydrate and peptide preparation In Enzymes in Nonqueous Solvents: Methods and Protocols (Vulfson, E.N., Halling, P.J & Holland, H.L.,...
- 9
- 497
- 0
Báo cáo khoa học: Identification of malic and soluble oxaloacetate decarboxylase enzymes in Enterococcus faecalis potx
... Remarkably, MLE and OAD proteins contain the same conserved amino acids found in the active site of previously characterized MEs, including the catalytic tyrosine and lysine residues involved in the acid–base ... variations in malic and OAD activities (not shown) It was previously suggested that Es coli ME may be involved in amino acid and ⁄ or lipid biosynthesis Bearing that in mind, we examined whether ... the divalent cation-binding residues, and two Rossman domains (GXGXXG) implicated in cofactor binding [1] (Fig 1B) For this reason, in this study we refer to MEs, MLEs and OAD enzymes as members...
- 12
- 271
- 0
Báo cáo Y học: A new conceptual framework for enzyme catalysis Hydrogen tunneling coupled to enzyme dynamics in flavoprotein and quinoprotein enzymes docx
... kinetic work on H-transfer by tunneling in quinoprotein and flavoprotein enzymes that catalyse the oxidation of a number of amine substrates, and computational studies of enzymic H-tunneling in ... (Wavefunction Inc., Irvine, CA, USA); the entire protein (including iminoquinone) was then protonated and solvated, and energy minimized Next the QM region was defined as comprising the sidechain of the ... computational studies of enzymic H-tunneling in these enzymes QUINOPROTEIN AND FLAVOPROTEIN AMINE DEHYDROGENASES The quinoprotein and flavoprotein amine dehydrogenases are ideally suited to studies...
- 7
- 359
- 0
Báo cáo y học: "Chlamydia trachomatis Infection of Human Trophoblast Alters Estrogen and Progesterone Biosynthesis: an insight into role of infection in pregnancy sequelae." doc
... determining the capacity of C trachomatis to initiate productive and transferable infection in trophoblast This involved the time course harvesting and purifying of EBs from infected trophoblast and ... sustain the growth of fetus We report a decline in estrogen and progesterone in chlamydial infected trophoblast and an accompanying positive feedback up regulation in the rate limiting enzymes in ... according 225 to manufacturers instructions Band intensity was determined using Gel Logic 200 (Kodak, Chicago, IL) Protein Estimation Protein assay on each sample was done by the bicinchoninic...
- 9
- 511
- 0
báo cáo khoa học: " Berry skin development in Norton grape: Distinct patterns of transcriptional regulation and flavonoid biosynthesis" docx
... encoding enzymes in the biosynthesis of flavonoids, lignin, anthocyanins and proanthocyanidins were expressed preferentially in the berry skin of grapevine [9] These genes include PAL, C4H, and ... Accumulation kinetics of the proanthocyanidins catechin, epicatechin, and epigallocatechin during V vinifera ’Cabernet Sauvignon’ (blue dashed line) and V aestivalis ’Norton’ (red solid line) berry skin ... anthocyanin amount at 112 DAB, followed by peonidin (14%), delphinidin (10%), petunidin (6.8%) and cyanidin (2%) Overall, in harvestripe berries, the total anthocyanin content in Norton berry skin...
- 23
- 303
- 0
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 1 ppsx
... mechanical, including photocopying, microfilming, and recording, or by any information storage and retrieval system, without permission in writing from the publisher Current printing (last digit): 10 PRINTED ... such as serine, phenylalanine, proline, methionine, and cysteine by birnessite, and the role of pyrogallol in influencing their mineralization have been investigated (152,153) Nitrogen mineralization ... Activities in Soil Enzyme Enzymes involved in C transformations Xylanase Cellulase Invertase β-Glucosidase β-Galactosidase Enzymes involved in N transformations Protease (casein-hydrolyzing proteases)...
- 46
- 555
- 0
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 2 pptx
... continuous measurement of enzymatic activity Products of substrates containing aromatic amines, including the commonly used 7-amino-4-methylcoumarin, 7-amino-4-trifluoromethylcoumarin, and 6-aminoquinoline, ... cells and from protozoan grazing on bacteria Ectoenzymes and extracellular enzymes (in contrast to intracellular enzymes) react outside the cell, and most of them are hydrolases The ectoenzymes ... information that helps in understanding basic processes of decomposition and microbial activity in both freshwater and marine ecosystems II ORIGIN AND ASSOCIATION OF ENZYMES WITH AQUATIC MICROORGANISMS...
- 38
- 511
- 0
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 3 pdf
... polymers at midchain, is not surprising Bacteria typically can express a range of both exo- and endo-acting extracelllar enzymes These enzymes typically have distinct domains for binding and cleavage ... Cottrell and colleagues should prove to be a promising means of investigating and identifying enzymes available to the vast majority of marine organisms that have not been isolated in pure cultures Investigations ... 10:570–576, 1965 KM Khailov, ZZ Finenko Organic macromolecular compounds dissolved in sea-water and their inclusion into the food chains In: JH Steele, ed Marine Food Chains Edinburgh: Oliver & Boyd,...
- 35
- 594
- 0
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 4 potx
... strains The DAPGϩ strain caused a significant decrease in the shootto-root ratio in comparison to that of the control and other inoculants, indicating plant stress F113 increased soil alkaline ... of Jordan and associates (16) They evaluated microbial measurements (including acid and alkaline phosphatase activities) as indicators of soil quality in long-term cropping practices in historical ... Variations in K m values obtained from the forest and grassland soils suggest that the origins of the enzymes were different in the two soils Therefore, such kinetic studies can be a good indicator...
- 15
- 455
- 0
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 5 ppt
... long-chain poly-P to total Copyright © 2002 Marcel Dekker, Inc Figure Enzymes involved in P transport in AM roots poly-P was observed in the external hyphae, and short-chain poly-P was higher in ... molecules involved in fungal–plant interactions is crucial for a better understanding of symbiosis Copyright © 2002 Marcel Dekker, Inc In the following sections, some results obtained from studying ... peroxidase activity increased linearly with increasing P supply, suggesting a role of peroxidase in limiting AM infection in well-P-nourished plants (140) The analysis of catalase and ascorbate peroxidase...
- 27
- 420
- 0
7Microbial Enzymes in the Biocontrol of Plant Pathogens and aEnzymes in the Environment: Activity, Ecology and Applications - Chapter 7PestsLeonid Chernin and Ilan ChetThe ppt
... hydrolytic enzymes produced by B cinerea both in vitro and in vivo and inhibited infection caused by B cinerea (146) C Involvement of Fungal Enzymes in Induced Resistance To protect themselves against ... attachment, and de novo synthesis of a set of cell-wall-degrading enzymes that aid the parasite in penetrating the host and completing its destruction Lectins, the sugar-binding proteins or glycoproteins ... endochitinase Thus, a combination of the Cry protein and an endochitinase could result in effective insect control in transgenic systems in which the Cry protein is not expressed in a crystalline...
- 56
- 674
- 1
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 8 potx
... activity A variety of enzymes involved in essential carbon and nitrogen transformations, including dehydrogenase, have been assessed in metal contaminated soils in both laboratory and field studies ... levels, and interactions with other soil minerals and organic matter (5,12,28,55,66,73) In a detailed evaluation of 13 soil enzymes involved with carbon, nitrogen, phosphorus, and sulfur cycling enzymes, ... more useful in determining the means and kinetics of reactions associated with organic pollutant decomposition or the effects of management decisions relating to the sustaining or improving of soil...
- 22
- 429
- 0
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 9 potx
... sites, litter bags containing Pinus kesiya and Myrica esculenta leaves were placed in a young forest and bags containing Pinus kesiya and Alnus nepalensis leaves were placed in a mature forest Samples ... activities increased through time and with initial lignin content Chamier and Dixon (8) found that pectinolytic enzymes produced by hyphomycetes, the principal fungal decomposers of plant litter in aquatic ... site and placed in litter bags In using the approach of Sinsabaugh et al (73) and Jackson et al (29), confined and in situ POM samples were assayed monthly for β-glucosidase, β-N-acetylglucosaminidase,...
- 17
- 373
- 0
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 10 docx
... been introduced over the years; these include vanilin, indulin, ferrulic acid, and, most importantly, 14 C-labeled synthetic lignins Various fungal enzymes are involved in lignin degradation, including ... soils and horizons; for determining the effects of pollution and changes in climate and land use; and for using biomass data in decomposition models (13) The mycelium is often well hidden in soil ... By incubating identical litter types in different climatic zones and by placing litter of Copyright © 2002 Marcel Dekker, Inc different origin in the same climate, interesting decay patterns and...
- 18
- 521
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008