emt like phenotype through regulating zinc finger e box binding homeobox 2 zeb2 expression

Báo cáo sinh học: "MiRNA-205 modulates cellular invasion and migration via regulating zinc finger E-box binding homeobox 2 expression in esophageal squamous cell carcinoma cells" pptx

Ngày tải lên : 18/06/2014, 19:20

... the miR-10a expression levels were decreased in ESCC cell lines (OE21, TE5, TE8, TE10, and TE11) compared to Het1A cells but the other cell lines (Caco2 and Jurkat) had more decreased expression ... inhibitor transfection leaded to enhanced expression of zinc finger E- box binding homeobox (ZEB) but not ZEB1 in OE21 cells (C) The downregulation of miR -20 5 decreased cellular E- cadherin expression, ... differentiation, respectively The miR -20 5 expression in ESCC tumor samples was assessed using real-time RT-PCR There were no significant differences in the relative miR -20 5 expression levels between...

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Báo cáo khoa học: The Promyelocytic Leukemia Zinc Finger (PLZF ) gene is a novel transcriptional target of the CCAAT-DisplacementProtein (CUX1) repressor ppt

Ngày tải lên : 29/03/2014, 21:20

... the ‘Fonds de la Recherche en Sante du Quebec’ (FRSQ) and member of the FRSQ-funded ‘Centre de ´ Recherche Clinique Etienne Lebel’ References Kunkel EJ, Campbell JJ, Haraldsen G, Pan J, Boisvert ... were normalized with Renilla values Results obtained in triplicate were reported as a percentage of the controls (means ± SD) and are representative of three independent experiments (E) The percentage ... 2A) In order to better characterize the nature of the different patterns for the CUX1-retarded complexes, some sites representative for these different patterns were analyzed further The introduction...

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báo cáo hóa học:" Ovarian cancer plasticity and epigenomics in the acquisition of a stem-like phenotype" pptx

Ngày tải lên : 20/06/2014, 07:20

... been extensively reviewed [ 123 ] Epigenetics-based gene therapy One therapeutic approach for targeting chemoresistance may be to reverse the epigenetic marks in chemoresistant cells Small interfering ... question remains: Are miRNAs differentially expressed as a consequence of the cancer state, or does cancer cause the deregulated expression of miRNAs? Both scenarios are likely true; miRNA have been ... Dominant-negative histone H3 lysine 27 mutant derepresses silenced tumor suppressor genes and reverses the drug-resistant phenotype in cancer cells Cancer Res 20 06, 66:55 82- 5591 Wiencke JK, Zheng S,...

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Báo cáo y học: "The tandem CCCH zinc finger protein tristetraprolin and its relevance to cytokine mRNA turnover and arthritis" pot

Ngày tải lên : 09/08/2014, 01:24

... polymorphisms were identified in the protein-coding domains by resequencing; all were in exon Three of the SNPs were identified by resequencing the EGP subject DNA These SNPs were present at frequencies ... be explained Genetic modifiers of the TTP-deficiency syndrome 25 2 We and others have evaluated the TTP-deficiency phenotype further by interbreeding the mice with other potentially informative ... Other interbreeding experiments are either under way or have not yet been published For example, J Rivera-Nieves has crossed the TTP-deficient mice with the TNF-α ∆ARE mice alluded to above (personal...

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Báo cáo y học: "Peripheral blood but not synovial fluid natural killer T cells are biased towards a Th1-like phenotype in rheumatoid arthritis" pptx

Ngày tải lên : 09/08/2014, 06:22

... and seven RA patients by immunomagnetic selection Positively selected cells were tested for α-GalCer reactivity to ensure the NKT cell nature of the cells The presence of CD4 was assessed by ... underlying bone [19] Autoreactive Th1 T cells are believed to play a major role in the disease process [20 -22 ] In RA patients, the frequency of NKT cells is decreased, but the functional characteristics ... in 23 RA patients (mean age 52. 1 ± 2. 0 years, 11 males and 12 females, mean disease duration 8.0 ± 1.6 years), who were diagnosed in accordance with the criteria of the American College of Rheumatology...

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Báo cáo y học: " PRDM9 points the zinc finger at meiotic recombination hotspots" potx

Ngày tải lên : 09/08/2014, 20:21

... surprising degree of variation [7] Not only is repeat number variable (11 to 14 repeats), but amino acid differ ences between the repeats are also extremely frequent Specifically, of the 24 amino ... from the Medical Research Council Published: 26 February 20 10 References Kauppi L, Jeffreys AJ, Keeney S: Where the crossovers are: recombination distributions in mammals Nat Rev Genet 20 04, 5:413- 424 ... determinants Indeed, of 22 inferred human hotspot loci at which there is also conservation of the 13 bp motif in both species, only one revealed evidence for conserved usage in the chimpanzee [8] Given that...

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Báo cáo y học: "Defining potentially conserved RNA regulons of homologous zinc-finger RNA-binding proteins" ppsx

Ngày tải lên : 09/08/2014, 22:23

... were generally enriched, possibly reflecting weaker interaction of respective messages with Gis2p Gis2p binds to GAN repeats within coding sequence of target mRNAs We next wondered whether there ... processing and nucleolar proteins were significantly decreased in GIS2 overexpressing cells (Figure 6b) In these cases however, the expression levels of non-Gis2p targets assigned to the respective ... shown in blue The number of features for which relative expression was selectively increased is indicated within the red circle, features with decreased expression are within green circles (cutoff:...

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báo cáo khoa học: " Chemically synthesized zinc finger molecules as nano-addressable probes for double-stranded DNAs" ppt

Ngày tải lên : 11/08/2014, 00:22

... places to enable the folded zinc finger to bind either at the "AAA"-DNA sequence site or at the "GCG"-DNA sequence site (see M & M, bold and underlined) The custom-made peptides were modified with ... zinc finger motifs of the SP1 -zinc- finger as a template for our chemically synthesized zinc finger probes, which were of the C2H2 type (Figure 1) The DNA -binding region was altered in two places ... chamber (Figure 2) The binding and dissocia- tion events were measured in real time and binding curves (binding signal vs time) were obtained (Figures and 4) The curves were fitted using in-house...

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Báo cáo khoa học: " Inactivation of respiratory syncytial virus by zinc finger reactive compounds" ppsx

Ngày tải lên : 12/08/2014, 04:21

... future vaccine development Results Zinc- finger- reactive compounds are effective against RSV as measured by their effect on infectivity Three compounds were chosen for evaluation of their effect ... finger motif of the M2-1 protein of RSV Therefore, we have tested some of the zinc finger reactive compounds that were discovered as active against HIV-1 and MuLV in the experiments using respiratory ... sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) either in the presence or in the absence of b-ME The proteins were then analyzed by Western blot using IgY antibodies against RSV antigens (Figure 4) M2-1...

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Báo cáo y học: "Mutagenesis analysis of the zinc-finger antiviral protein" ppt

Ngày tải lên : 12/08/2014, 23:23

... Rat2-NZAP-Zeo cells have been described previously [4] The pBabe-NZAPZeo-myc based constructs expressing NZAP-Zeo-myc mutants were packaged into MLV vector to transduce Rat2 cells The cells were selected ... cells (upper panel) The tetracycline induced protein expression was confirmed by Western blotting (lower panel) The fold inhibition data are mean + SD of three independent experiments both the ... Another possibility is that the mutations interfered with the folding of the protein, resulting in very low levels of expression The expression levels of the zeocin resistant mutants were measured...

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Báo cáo y học: " Identity of zinc finger nucleases with specificity to herpes simplex virus type II genomic DNA: novel HSV-2 vaccine/therapy precursors" pps

Ngày tải lên : 13/08/2014, 16:20

... recognition domains (F1, F2/F3, F2, F1) alongside the respective site specific sequence within the HSV -2 genome, are presented in table Residues -1 to (numbered relative to the start of the helix) ... the host-genome Zinc fingers (Zif or ZF), like those specific to the nucleotide sequences of the complete HSV -2 genome (provided in additional file 1) presented in additional file 2, are protein ... http://www.tbiomed.com/content/8/1 /23 specified nucleotide sequence, zinc fingers have previously been used to direct small molecules into unique sequences within the human genome including genes of integrated...

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Báo cáo y học: " Proviral HIV-genome-wide and pol-gene specific Zinc Finger Nucleases: Usability for targeted HIV gene therapy Misaki Wayengera" ppt

Ngày tải lên : 13/08/2014, 16:20

... Early Careers’ Investigators (YECI) of the Global HIV Vaccine Enterprise Received: 26 May 20 11 Accepted: 22 July 20 11 Published: 22 July 20 11 References Gallo RC, Montagnier L: The Discovery of ... transport element lead to enhanced HIV-1 Gag expression in a contextdependent manner Nucleic Acids Res 20 00, 28 :901-910 48 Kauder SE, Bosque A, Lindqvist A, Planelles V, Verdin E: Epigenetic regulation ... conserved cysteine and histidine residues Residues to of the alpha-helix (numbered relative to the start of the helix) are responsible for the specific recognition of triplets of DNA sequences through...

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Test E. 7 HK II (2)

Ngày tải lên : 31/05/2013, 00:21

... KỲ II MÔN ANH I/ (2 điểm) Mỗi câu cho 0,5 điểm 1.worked 2. goes 3.are playing 4.will visit II/ (3 điểm) Mỗi câu cho 0,5 điểm 1.the matter 2. feel twice she was bought how III/ (2 điểm) Mỗi câu cho ... F F T F IV/ (2 điểm) Mỗi câu cho 0,5 điểm 1.-c -d -a 4.-b V/(1 điểm) Mỗi câu cho 0,5 điểm 1.We went to the movies last night but the film was boring My sister doesn’t really like watching sports...

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Test E. 8 HK I (2)

Ngày tải lên : 31/05/2013, 00:21

... 1) She is 14 years old 2) She lives in an apartment 3) yes , she does 4) she has 1000 stamps V ) Viết câu cho 1điểm 1) Lan likes learning English 2) She has studied English for years THE END ... cho 0.5điểm )went 2) is cooking 3) playing 4) have you known II ) Mỗi từ chọn cho 0.5điểm 1) B has lived 2) B went 3) A are / thingking 4) C to go III ) Nói câu cho 1điểm 1) 1d 2c 3a 4b IV )...

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Test E. 7 HK I (2)

Ngày tải lên : 01/07/2013, 01:26

... 7o’clock she usually talks to her friends yes she is Her favorite subjects are English and Math IV/ (2 điểm) Mỗi câu cho 0,5 điểm 1.At from of with V/(1 điểm) Mỗi câu cho 0,5 điểm 1.He usually goes to ... I/ (2 điểm) Mỗi câu cho 0,5 điểm 1.watches 2. are skipping 3.going 4.will visit II/ (3 điểm) Mỗi câu cho 0,5 điểm 1.on 2. play fewer drink 5.who 6.reading III/ (2 điểm) Mỗi câu cho 0,5 điểm They ... 1.At from of with V/(1 điểm) Mỗi câu cho 0,5 điểm 1.He usually goes to Lan ‘s house on weekend When you have English ...

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E 7 Unit5 B1,2

Ngày tải lên : 02/08/2013, 01:27

... be + V-ing Dùng để hỏi đáp làm ? III PRACTICE 1 IV PRODUCTION * Learn lesson by heart * Write the four sentences about the pictures that you has described * Prepare new lesson :Lesson 5: B2 ... GROUPS GUESS LETTER BY LETTER - IF CHOOSING WRONG LETTER, FALLING DOWN ONE STEP - WHO IS FAR THE SHARKS IS THE WINNER Warm up : A B BE CAREFUL ! I Vocabulary : Play marble Skip rope Play blindman's ... GRAMMAR • Set the scene : are A: What…… they doing ? B: They are ……………… chatting Họ làm ? are A: What…… they doing ? Meaning chatting B: They are ……… Form Use Họ tán gẫu A: What + be + S + doing...

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E 7 Unit3 A 2,3

Ngày tải lên : 02/08/2013, 01:27

... C/USE:ĐƯA RA LỜI KHEN HOẶC LỜI PHÀN NÀN II PRACTICE:WORD CUE DRILL Terrible/Sink Interesting/Shower Lovely/Dog Delightful/Tub Delicious/Food Awful/ Day GUESSING GAME: IS THERE+ A/AN+N? ARE THERE ... THERE ANY+ N(S/ES)? IV/ HOMEWORK: Make sentences with " What+ a/an+ adj+N! A.The film is very interesting B.This shower is very awful C.The tub is very terrible D.The dog is very lovely ... DAY! IT'S VERY COLD B:COME AND SIT HERE, PLEASE! A: WHAT A LOVELY ROOM! A; WHAT AN AWFUL DAY! IT'S VERY COLD B:COME AND SIT HERE, PLEASE! A: WHAT A LOVELY ROOM! 2/ CONCEPT CHECK: A/MEANING: B/FORM:...

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NGHIÊN cứu CHẾ tạo KHÁNG THỂ QUA LÒNG đỏ TRỨNG gà để PHÒNG CHỐNG TIÊU CHẢY và SƯNG PHÙ đầu DO e coli ở lợn (2)

Ngày tải lên : 04/08/2013, 15:30

... theo đờng dới da, tiêm theo đờng tiêm phúc xoang tối thiểu 0 ,2 ml/con Dựa vào tiêu chuẩn lựa chọn đợc 13 chủng có độc lực chuột là: E3 , E7 , E9 , E1 2, E1 4, E1 5, E1 9, E2 0, E2 2, E2 4, E2 5, E2 7, E2 8 ... Enterotoxigenic Escherichia coli (ETEC), Enteropathogenic Escherichia coli (EPEC), Verotoxigenic Escherichia coli (VTEC) Adherencia Enteropathogenic Escherichia coli (AEEC) E coli gây bệnh tiêu chảy phù ... 1/ 128 1/1 12 Chuột tiêm E2 4 1/4, 1/4, 1 /2, 1/8 1/4,5 Chuột tiêm E2 5 1 /25 6, 1/ 128 , 1/64, 1 /25 6 1/176 Chuột tiêm E2 7 1/ 32, 1/64, 1/64, 1/ 128 1/ 72 Chuột tiêm E2 8 1/ 128 , 1 /25 6, 1 /25 6, 1/ 128 1/1 92 Chuột...

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Motivating grade 11 students to learn english through examination taking strategy training at yen thanh 2

Ngày tải lên : 07/09/2013, 13:43

... are the strategies the testtaker uses to take the test, including language use strategies and test-wiseness ones Language use strategies are considered close to the learner’ competence while test-wiseness ... problems in understanding the questionnaire items In this study, two questionnaires were designed to gather the needed dada: one delivered before and one delivered after the treatment 2. 4.1.1 Pre- ... process Moreover, when teachers have expectations in their students’ ability, the learners are likely to reach high level of achievement and feel more confident Learners tend to perform at a level that...

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