... -MASSPTKILCDAGESDLCRDDAAAFLLKFVAIASIL 1:MFFIDVLWKLFPLYLFGSERDYLSETESILKIVPETMAAASSLSILCDAGEPDLCRDDSAAFLLKLVAIASIF TjZNT1 TjZNT2 37:LAGVAGVAIPLIGKNRRFLQTEGNLFVAAKAFAAGVILATGFVHMLAGGTEALTNPCLPDYPWSKFPFPGFFA ... Thlaspi caerulescens asa model system Ann Bot 102, 3–13 Weber M, Harada E, Vess C, Roepenack-Lahaye E & Clemens S (2004) Comparative microarray analysis of Arabidopsis thaliana and Arabidopsis halleri ... transport FEBS Lett 581, 2263–2272 N-terminus of TjZNT2 is involved in ion selectivity Tomatsu H, Takano J, Takahashi H, Watanabe-Takahashi A, Shibagaki N & Fujiwara T (2007) An Arabidopsis thaliana...
... sensory nucleus and the lateral parabrachial nucleus of the brainstem A small number of scattered NPS-positive cells were found in other brain areas, such as amygdala and hypothalamus The NPS-producing ... produce a transient increase in intracellular free Ca2+, indicating that NPS might be an excitatory transmitter in vivo by elevating intracellular Ca2+ A radiolabled analog of NPS (125I-labeled ... profound increase in locomotion, measured as the total distance traveled over one hour It is well known that animals naturally show increased exploratory activity when they are exposed to anovel environment...
... IL-2 and IFN -a for months as an adjuvant therapy; patient received IFN -a for lung metastasis, and patient received highdose IL-2 for metastatic mediastinal lymphadenopathy followed by radical lymph ... subcutaneously as easy and practical as they may be–reverse gradually as soon as vaccinations are completed Accordingly, we believe that such treatment needs to be continued in order to maintain ... manuscript EA participated in the patients care RI carried out the immunoassays AT carried out the immunoassays BG participated in the patients care VEH participated in the patients care WML analyzed...
... peptide After incubation, an assay for cell viability was carried out using Living Cell Count Reagent SF (Nacalai Tesque) according to the manufacturer’s protocol Absorbance was measured at a wavelength ... then analyzed by phase-contrast (DIC), fluorescence (TAMRA-red) or merge image (DIC and TAMRA-red) All images were taken using confocal laser scanning microscopy as described in Methods All scale ... RQIKIWFQNRRMKWKKKAYAAAGNSYFK (Antp-TPR mutant 1), RQIKIWFQNRRMKWKKKAYARIGNSGGG (Antp-TPR mutant 2), and RQIKIWFQNRRMKWKKRKFSAAIGYNKY (Antp-scramble) Materials Anti-Hsp90 and anti-Hsp70 antibodies, human recombinant...
... AAA GA-3’; GAPDH fwd 5’-ACA CCC ACT CCT CCA CCT TT-3’, GAPDH rev 5’-TGC TGT AGC CAA ATT CGT TG-3’ To compare and quantify different measurements a cellular cDNA was used as standard and the amount ... esRNA was analyzed by PCR (C) Total RNA was isolated from amniotic fluid and urine exosomes and analyzed via an Agilent Bioanalyzer The results show that exosomes contain variable amounts of 18 and ... 0.5 0.0 pg RNA relative to cellular standard 0.8 GAPDH 0.6 0.4 0.2 0.0 exosomes 10 11 12 membrane blebs Aas e N R + -R N asas N R + R + e AA e A e as N as N -R + R N as e e AA B 320 bp 382...
... AAA GA-3’; GAPDH fwd 5’-ACA CCC ACT CCT CCA CCT TT-3’, GAPDH rev 5’-TGC TGT AGC CAA ATT CGT TG-3’ To compare and quantify different measurements a cellular cDNA was used as standard and the amount ... esRNA was analyzed by PCR (C) Total RNA was isolated from amniotic fluid and urine exosomes and analyzed via an Agilent Bioanalyzer The results show that exosomes contain variable amounts of 18 and ... 0.5 0.0 pg RNA relative to cellular standard 0.8 GAPDH 0.6 0.4 0.2 0.0 exosomes 10 11 12 membrane blebs Aas e N R + -R N asas N R + R + e AA e A e as N as N -R + R N as e e AA B 320 bp 382...
... the ELISA experiments and participated in analysis of data CA performed the statistical analysis and the clinical associations AS participated in the analysis and interpretation of data and in ... study, participated in its design and coordination, and drafted the manuscript All authors read and approved the final manuscript Acknowledgements An association between serum AECAs and psychosis ... identify a possible molecular target of AECAs in an SLE patient with active psychosis, analyzing the same population of patients as in the previous investigation [5], we demonstrated an association...
... by screening an aplastic anemia patient for candidate antigens using a Clontech human fetal liver cDNA expression library and it was concluded that seven out of 18 aplastic anemia patients were ... anti-kinectin and anti-PMS1 antibodies in Japanese aplastic anaemia patients Br J Haematol 2005, 128:221-223 39 Imai H, Nakano Y, Kiyosawa K, Tan EM: Increasing titers and changing specificities of antinuclear ... 1996, 7:1015-1024 Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs Nucleic Acids Res 1997,...
... genome (release hg19) accounted for miRNAs annotated in mirBase (release 16) Other small RNA species, such as piwi-interacting RNAs (piRNAs) and small nucleolar RNAs (snoRNAs), were also identified ... correspond to any annotated small RNA Our small RNA cloning strategy only captures small RNAs that are, as miRNAs, 5’-phosphorylated and, Page of 13 thus, eliminates RNA degradation products generated ... renilla and firefly luciferase activities were assayed with the Dual Glo Luciferase Assay System (Promega) and measured with a luminometer (Luminoskan Ascent, Thermo Scientific, Waltham, MA, USA)...
... vision, as others have also adopted this strategy asa way forward in molecular analysis Alagaratnam et al are utilising Bayesian approaches to pursue muscular dystrophy diagnosis [223] Similarly, ... other arbitrary measures for disease classification Adopting a systems biology approach, whereby a disease defining molecular fingerprint is analysed, would increase the accuracy of disease diagnosis, ... 51(12):2333-2340 Okamoto M, Kawabe T, Iwasaki Y, Hara T, Hashimoto N, Imaizumi K, Hasegawa Y, Shimokata K: Evaluation of interferon-gamma, interferon-gamma-inducing cytokines, and interferongamma-inducible...
... neurofilaments and the phosphorylation is required in maintaining axonal morphology Cdk5 was originally isolated from the brain asa neurofilament kinase to catalyze the KSP phosphorylation at the tail ... co-immunoprecipitation approach in our search for novel interacting proteins Using the former approach, the catalytic α subunit of protein kinase CK2 (formerly known as casein kinase 2) was isolated from rat ... interacts with proteins such as fibroblast growth factor and HSP-90 that may directly alter or stabilize its catalytic activity (Skjerpen et al., 2002; Miyata and Yahara, 1995) Studies have demonstrated...
... inhibition of ataxia telangiectasia mutated (ATM) and ATM and Rad3-related (ATR) protein kinases though detail mechanism has yet being elucidated (d'Adda di Fagagna et al 2003; Takai et al 2003) Telomeres ... have an exposed 53BP1 interaction site thereby activates the DNA damage surveillance pathway TRF2 was proposed as an ATM inhibitor as it was found to be able to physical interacts with ATM at ... telomerase inhibitors They vary in form of screening targets as well as the screening approaches A summary of telomerase targeted cancer therapy and screening that has being published is available...
... strains of Malaria parasites (Plasmodium falciparum) and common serotypes of Dengue viruses A computational algorithm was created that greatly automated the design and assessment of these Mz-based ... countries where Malaria is most devastating [Mens et al, 2007] As such, PCR based methods are excluded from consideration asa field-ready rapid diagnostic kit for Malaria [McNamara et al, 2004] In ... Africa, occasionally in South East Asia and PNG., and are all CQS 4) Plasmodium malariae occurs at low frequency in patchy distribution worldwide, and are CQS In Singapore, about 100 to 300 cases occur...
... GGATCCGATCGTGTCGAACGAGATCA 60.0 KHWTTSS2P3 GGATCCGGCATCGACGGTATTCT 66.9 KHWTTSS2P4 AAGCTTATATCGCCGGGATAGCGTA 66.9 BTTTSS2P1 aaGAATTCGGTGGCCTCCAGAAACAGT 55.0 BTTTSS2P2 aaGGATCCGAGGACCCGCATGACAAG 55.0 ... GAATTCCGAACCGCTTTGTGATAACC KHWTTSS1P2 GGATCCGATCTTGAGCAGATGCTTG 60.7 KHWTTSS1P3 GGATCCGCCACGATATCCTCGAAAAG 60.7 KHWTTSS1P4 CTGCAGAGCTACGCCGTGAACGTATT 60.7 KHWTTSS2P1 GAATTCCTCGAACCGTCCATCGTC 60.0 KHWTTSS2P2 GGATCCGATCGTGTCGAACGAGATCA ... that exposure to natural disaster such as tsunami can be a relevant risk factor (Athan et al., 2005) 16 B pseudomallei can also cause disease in cattle, pigs, goats, horses, dolphins, koalas,...
... clinical trial, assigned according to the randomization list The appropriate package was handed to the particular participant Statistical data analysis and power calculation Statistical data analysis ... GAC AGA ATG TAC ACT TCA 24 nt tuf-S CCA TCA AGC CGC ACA CCA AGT TCG 24 nt Lacto-F2 TGG AAA CAG ATG CTA ATA CCG 21 nt Lacto-R2 CGT CCA TTG TGG TAG ATT CCC T 22 nt Lacto-S CTG AGA CAC GGC CCA WAC ... WAC TCC TAC GG 26 nt F_reut_IS ACC GAG AAC AAC GCG TTA TTT 21 nt R_reut_IS CAT AAC TTA ACC TAA ACA ATC AAA GAT TGT 30 nt P_reut_IS ATC GCT AAC TCA ATT AAT 18 nt Ampl Lit 159 bp (Yang et al., 2002)...
... generated from a pulsed scan and required for image generation Image manipulation was carried out using the manufacturer’s software, Galaxis To increase the accuracy of the assessment, all three ... surrounding bony areas facilitating a variable response to tooth movement [5] In another study, it has been reported that low magnitude mechanical signals are “anabolic” to bone when applied at a high ... planes (sagittal, axial, and coronal) were utilized CBCT images were taken at two time frames; once at the start of treatment (T1) and again after six months of treatment (T2) Measurements of all...
... 0) and condensation ( m phase < 0) is assumed Where m phase is mass transfer: for evaporation & & & & ( m phase = mevap ) and for condensation ( m phase = mcond ) (kg/s) So that the mass balance ... support additional active area and generate increased volumetric power density The height of the gas diffusion layers (GDLs) decreases along the main flow direction and this leads to improve the gases ... field, mass transport and electrochemistry in an air-breathing cathode of a planar PEM fuel cell In their results, electrochemical/mass characteristics such as flow velocities, species mass fraction,...
... proposed and tested ?his filter cpexates atso as reactive leading and lagging power source However, au maease of readive power c a w muease of voltampere power ofthe adive filter [I] F.Blaabjer& ... non itr activepower ?his filter is specially designed for drive applications with classical amveater syjian (ad&) with diode bridge redifier as an input of an eledric drive ?he total iaput t ... Total input phase ament with phase.voltage ul Figure Sa: Redifier phase ament il and utility phase voltage u1 & taka &om the supply line CONCLUSIONS l h e re&fier ament il is lagging the voltage...
... breeders served as untreated controls Sham-treated animals underwent all preparations for ultrasound treatment as treated animals: anesthesia was administered and maintained at - 2.5% isoflurane/oxygen, ... ME7410 transducer only produced MHz ultrasound Treatment apparatus A Plexiglas cylinder was used as the ultrasound chamber (70 mm diameter, 25 mm tall) The bottom of this chamber was a single layer ... scrotal fur was shaved, a ligature was used to retain the testes in the scrotum, room temperature coupling medium was placed in the treatment chamber, animal was placed on the treatment apparatus...