effect of pas on oxidative stress
Cloning and characterization of diamine oxidase and glutamate decarboxylase genes of mustard (brassica juncea) and their roles in shoot morphogenesis in vitro
... pGreenGFP 77 Construction of DAO-AS and DAO-S 79 Construction of chimeric genes consisting of GADS, GAD-AS and tGAD 80 Schematic representation of constructs carrying the gene fusions of the GUS coding ... exogenous stimuli 188 Effects of overexpression and downregulation of DAO and GAD RNAs on shoot regeneration in vitro 192 6.1 Introduction 192 6.2 Results 194 6.2.1 Construction of sense and antisense ... he also gives me professional support, help and inspiration i Table of Contents Page Acknowledgements i Table of Contents ii List of Abbreviations x List of Figures xiii List of Tables xvii Summary...
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Recombinant DNA I - Basics of molecular cloning Polymerase chain reaction cDNA clones and screening
... copies in the cell A common plasmid cloning vector: pUC lacZ mulitple cloning sites pUC ApR ColE1 origin of replication Lac+, or blue colonies on X-gal in appropriate strains of E coli High copy number ... information becomes available, the uses of PCR expand • With appropriate primers, one can amplify the desired region from even miniscule amounts of DNA • Not limited by the distribution of restriction ... function, expression, structure, etc • Enzymology - place fragment encoding a particular region of a protein in an expression vector • Population genetics - examine polymorphisms in a population...
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báo cáo khoa học: " Sequencing analysis of 20,000 full-length cDNA clones from cassava reveals lineage specific expansions in gene families related to stress response" docx
... Comparison of the annotation of 6566 cassava sequences with putative one-to-one orthologs and 4313 sequences without Comparison of the annotation of 6566 cassava sequences with putative one-to-one ... and clone id information to select groups of two or more contigs formed by sequence reads of clones with a second read assembled in only one contig Secondly, we performed a BLASTN analysis of the ... indirectly to stress response The comparison of the annotation of sequences with and without RBHs that we made for the detection of stressinduced genes is based on the following assumption: if there...
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Báo cáo y học: "ISOLATION OF CHLAMYDIA PNEUMONIAE FROM SERUM SAMPLES OF THE PATIENTS WITH ACUTE CORONARY SYNDROME"
... lack of standardized criteria for laboratory diagnostics of chronic C pneumoniae infection as well as contradictory information about distribution of the pathogen throughout of the tissues of human ... Taq polymerase First run of amplification was conducted under cycling conditions consisting of an initial denaturation at 95°C for min, followed by 45 cycles of denaturation at 95°C for 30 sec, ... CpPLD are among promising candidates for immunological diagnostics of C pneumoniae infection (8, 9) However, changed antigenic profile of C pneumoniae during persistent colonization in human...
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Báo cáo khoa học: Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis A key factor participating in natural rubber biosynthesis pdf
... the amount of IPP incorporation into the toluene/hexane extracts was similar to that of the control experiment with WBP alone On the other hand, addition of WBP to the homogenate of BL21(DE3)/pETHRT2 ... the effect of the HRT2 protein on rubber transferase activity in coincubation with WBP The activity increased as the concentration of HRT2 was raised, until the activity reached saturation The effect ... extracted and cloned into pT7Blue T vector One of the cDNA fragments, which contained regions homologous to those of the conserved regions II, III and IV of cis-prenyltransferase was designated...
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Báo cáo khoa học: Isolation of a putative peroxidase, a target for factors controlling foot-formation in the coelenterate hydra docx
... formation, is expressed in the peduncle region [24] Hence, analysis of the regulation of ppod1 expression should shed some more light on the mechanisms of pattern formation in the foot of hydra ... preparation of extracts, 30 mg of lyophilized H vulgaris (500–600 animals) or frozen foot pieces (about 1000) were sonified for · s on ice (Branson Sonifier 250) in a buffer consisting of 20 mM ... exchanger at a salt concentration of less than 100 mM with a yield of about 10% (Fig 3A) For a comparison the interaction of the foot-specific peroxidase with a cation exchanger, Mono S was tested...
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Báo cáo Y học: Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components ppt
... amount of small subunit to purify each component Therefore, to simplify the procedure of isolation and purification of mitoribosome, we constructed a plasmid containing the gene for Mrp4, one of the ... under native conditions However, their isolation conditions were suited for tagantibody and tag–calmodulin interactions In contrast, we performed affinity purification under the conditions suited ... course of evolution as in the case of an E coli mutant [27] In both yeast and mammals, about a half of the mrps are unique to mitoribosome and only a small fraction of them are reasonably conserved...
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Báo cáo khoa học: Biotinylation in the hyperthermophile Aquifex aeolicus Isolation of a cross-linked BPL:BCCP complex pptx
... BCCP contain a large number of charged residues and we observed inhibition of biotinylation at high salt concentrations, we presume that ionic interactions are involved in the formation of the ... apo-BCCPD67 The reaction was initiated by the addition of purified BPL to a final concentration of lM, and incubated at 70 °C for 30 The reaction was terminated by the addition of ice-cold trichloroacetic ... The final yield of apo-BCCPD67 was 5–10 mg per litre of cell culture and mg per litre of the holo-form Cloning, expression and purification of BCCPD67 K117L mutant Fig Purification of A aeolicus...
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Báo cáo Y học: Amphibian peptides that inhibit neuronal nitric oxide synthase The isolation of lesueurin from the skin secretion of the Australian Stony Creek Frog Litoria lesueuri docx
... interaction must be causing the inhibition of nNOS To con®rm this suspicion and to elucidate the general mechanism of inhibition, an analysis was conducted of the kinetics of the nNOS inhibition reaction ... tumour line testing programÕ of the US NCI (Washington) [12] Neuronal nitric oxide synthase inhibition Inhibition of nNOS was measured by monitoring the conversion of [3H]arginine to [3H]citrulline ... peptides of inhibitor groups 1±3, must therefore inhibit the formation of nitric oxide by either blocking one or more of the cofactor sites on nNOS or by some chemical modi®cation reaction with...
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Selection Guide for Vibration Isolation of HVAC Equipment pot
... Fire Station, Barrie, Ontario CA • Caledon OPP Station, Caledon (Toronto), Ontario CA • Casino Niagara • City of North Las Vegas Water Reclamation Facility, Las Vegas, NV • Cosmopolitan of Las Vegas ... Fire Station, Barrie, Ontario CA • Caledon OPP Station, Caledon (Toronto), Ontario CA • Casino Niagara • City of North Las Vegas Water Reclamation Facility, Las Vegas, NV • Cosmopolitan of Las Vegas ... Fire Station, Barrie, Ontario CA • Caledon OPP Station, Caledon (Toronto), Ontario CA • Casino Niagara • City of North Las Vegas Water Reclamation Facility, Las Vegas, NV • Cosmopolitan of Las Vegas...
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generation of cdna libraries
... above-mentioned reasons, cDNA libraries are preferred over mRNA libraries for genetic manipulations Conventional vs Novel Strategies for cDNA Generation The conventional method of the generation of cDNA ... 0.002% of the reverse transcription mixture suffices for the PCR reaction! The addition of too much starting material to the amplification reaction will lead to production of large amounts of nonspecific ... optimal conditions, in a volume of 10 µL Add a 10-µL mixture containing the selected dNTP(s) (e.g., dATP) at a final concentration of 0.4 mM, µL of the restriction buffer used for digestion, 0.5...
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Báo cáo lâm nghiệp: "Value production of poplar clones'''' ppt
... limit of 35 years but for the lowest site indexes it is at a considerably older age CONCLUSIONS The value production of the stands of poplar Methodology of the construction of the models clones ... production in the form of models of yield and assortment yield tables Value yield tables are their continuation The aim of this paper is to present the methodology of the construction of the models of ... Based on them the production tions for single assortments as well as the costs of of both clones is evaluated as well According to the production of final assortments of raw timber relation (1)...
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Báo cáo lâm nghiệp: "Effect of Melampsora larici-populina on growth and biomass yield of eight clones of Populus nigra" ppsx
... conditions using a 6-point scale: – no signs of rust infection on leaves; – small patches covered by uredia on a half of the Table Localization of parental trees from which the tested clones of ... coefficient of rank correlation (rs) This test was used to evaluate the stability of the degree of infection within the particular clones of black poplar in the years of observation, the relationship ... intensity of infection (3.6–4) was determined in clone This clone was significantly different (P < 0.05) in infection from clones 7, 107, 110, 152 The stability of the intensity of infection of black...
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Báo cáo lâm nghiệp: "Quality of wood in the stands of poplar clones" docx
... diameter of stand (cm) Fig Proportions of the quality classes of logs of clone Robusta with zero and 40% damage of stems of quality class A Fig Proportions of the quality classes of logs of clone ... classes of logs of clone Robusta with zero and 40% damage of stems of quality class B Fig Proportions of the quality classes of logs of clone I-214 with zero and 40% damage of stems of quality ... proportions of assortments (quality classes of logs) on sample plots and the proportions of assortments on these plots determined by assortment models Actual proportions of the assortments on sample...
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Báo cáo khoa học: "Isolation of Subgroup J Avian Leukosis Virus in Korea" potx
... reduces the rate of congenital transmission of a field strain of avian leukosis virus Poult Sci 1992, 71, 799-806 Crittenden, L B., S McMahon, M S Halpern, and A M Fadly Embryonic infection with the ... described (16) The cycling conditions of PCR were denaturation at 94℃ for min, followed by 30 cycles at 92℃ for 1min, 48℃ for 1min, and 72℃ for 1min A final extension was conducted at 72℃ for 5min ... ac tion (P CR) PCR was done for the detection of proviral DNA from cells infected with isolates DNA was prepared with the Qiagen DNA isolation kit (Qiagen Inc., USA) and about 10 0～150ng of genomic...
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Báo cáo khoa học: "Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use" ppsx
... Watson RR (ed.) Nutrition and heart disease pp 83-109, Churchill Livingstone, London, 1983 Gilliland SE, Nelson CR, Maxwell C Assimilation of cholesterol by Lactobacillus acidophilus Appl Environ ... Effect of Bifidobacterium longum HY8001 administration on human fecal bacterial enzyme and microflora Korean J Appl Microbiol Biotechnol 1999, 27, 267-272 16 Lee YW Effect of fermented milk on ... precipitation zone (19 mm) But only strains (12.8%) among Lactobacillus had BSH activity In vitro cholesterol-lowering test: Among isolated 100 strains of LAB, of Streptococcus strains, 11 of Lactobacillus...
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Báo cáo khoa học: "Micropropagation and ex vitro rooting of several clones of late-flushing Quercus robur " ppsx
... from 45 clones The in vitro were shoot productivity depended upon the clone; the number of shoots produced after months varied between 10 and more than 1000 clone There was also an effect of the ... gene conservation programs For long-term storage, cryopreservation methods are available (Jörgensen, 1990) In this article, we report the micropropagation of late-flushing Q robur from acorns of ... clones The influence of the genetic background of the mother tree is demonstrated by differences found in plant survival by the mean number of surviving plants/clone (table I) Depending upon...
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Báo cáo khoa học: "Border effects and size inequality in experimental even-aged stands of poplar clones (Populus)" pptx
... mea- n y concen- tration measures CV and G meet the axioms of permutation invariance, scale invariance and the Dalton-Pigou principle of transfers Mutual comparison of concentration measures ... the end of each growing season at Afsnee (table I), the group of clones FRI + BEA + RAS belonged to the taller clones on average; ROB was always the shortest The 95% confidence interval of BEA ... irrigation and fertilization were sufficiently supplied both on the plot and far beyond the unplanted alley, only canopy competition for light can be responsible for the development of a border...
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Báo cáo khoa học: "Crown architecture in relation to productivity of Populus clones in the Pacific Northwest, U.S.A." ppt
... that of proleptic branches for the R trichocarpa and hybrid clones in the middle of the season, but was only 1/5 the biomass of proleptic branches in clone 111-005 The ratio of total LA on sylleptic ... 35-40% of the total LA were carried on sylleptic branches in hybrid clones 44-136 and 11-11, respectively The LA on the current terminal of each of the clones remains a minor part of the total LA of ... the hybrid clones In clone 111-005 total LA on proleptic branches was always higher than that of their sylleptic counterparts The relative proportion of LA on sylleptic branches to LA on proleptic...
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Báo cáo khoa hoc:" Development and assignment of bovine-specific PCR systems for the Texas nomenclature marker genes and isolation of homologous BAC probes" ppt
... preparation A 4-fold genome equivalent bovine BAC library containing 105 984 clones was constructed in pBeloBAC11 (Eggen et al., submitted) Clones were pooled in 46 primary superpools of 304 clones ... non-pseudo autosomal region As a result, ZFY itself will serve as a marker of the Y chromosome in our panel 3.3 Isolation of bovine BAC clones For each marker gene, at least one BAC clone was identied ... Hopkins J.A., Sikela J.M., Use of untranslated sequences of human cDNAs for rapid chromosome assignment and conversion to STSs: implication for an expression map of the genome, Nucleic Acid Res...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose