... Cataract and a- crystallin mutations The mutation responsible for autosomal dominant congenital cataract, a common cause of infant blindness, localizes to the aA-crystallin gene (CRYAA) [60] An ... encourage cataract As a prelude to examination ofprotein recognition by modified a- crystallins, results obtained by mammalian two-hybrid analyses demonstrate that interaction of aA- and aB-crystallin ... a Nova Scotia Health Research Foundation ⁄ Canadian Institutes of Health Research Regional Partnership Plan Grant, anda Heart and Stroke Foundation of Nova Scotia Grant to THM anda NSHRF Student...
... presence and absence of ATP on the aggregation of CS In the absence of added MTB HSP 16.3, aggregation of CS increased after a short delay to reach a maximum after approximately 25 at 45 °C Ó ... Sherman for the kind gift of the monoclonal antibody IT-4 (a- 16 kDa), and S Yarfitz for technical assistance with the Multalin Sequence alignment program We also thank J Clark and C Ganders for ... with and without ATP over a 30-min period The aggregation of CS was measured with the addition of different concentrations of MTB HSP 16.3 and in the presence or absence of ATP and ATP analogs (A) ...
... prevention of nonspecific aggregation of partially denatured actin that can trap intact actin The 3D mutant prevents aggregation and by this means increases the quantity of available actin monomers and ... polymerization of intact actin and aggregation of heated actin Interaction of HSP25 with intact actin Fig Effect of heating on the kinetics of polymerization (A) and saltinduced increase of the ... the appearance of an additional peak at 7.65 mL on the elution profile Increase of the temperature of incubation was accompanied by the simultaneous increase of the peak eluted at 7.65 mL and...
... or has a more nonglobular shape Finite element analysis of the data [28] estimates a molecular mass of 201 kDa for TaHsp16.9C-I and 173 kDa for TaHsp17.8C-II These data are consistent with a more ... initial heatinactivation step Data points and error bars reflect the mean and standard deviation of three replicates Results Comparison of TaHsp16.9 C-I and TaHsp17.8 C-II To produce recombinant ... extended shape for TaHsp16.9C-I than for TaHsp17.8C-II and with a dodecameric organization for TaHsp16.9C-I and an oligomer of TaHsp17.8C-II containing 9–10 monomer units These data are in good agreement...
... concentration, and that mutant proteins equivalent to HspH(G11 4A) form unstable complexes Materials and methods Bacterial strains and plasmids Escherichia coli DH 5a, grown in LB (Luria–Bertani) medium, was ... T., Sasamoto, S., Watanabe, A. , Idesawa, K., Iriguchi, M., Kawashima, K., Kohara, M., Matsumoto, M., Shimpo, S., Tsuruoka, H., Wada, T., Yamada, M & Tabata, S (2002) Complete genomic sequence of ... the control sample (B) and the heat-treated sample (C) was collected, precipitated, separated by SDS-PAGE and stained with Coomassie blue Lanes and correspond to the peak that eluted at around...
... methanol, without prior permeabilization, and treated with DNase I (a c) or RNase A (d–f) Cells were costained with the RIKEN mAb anti-(aB-crystallin) (a and d) and YOYO-1 (b) or anti-Sm (e) Panels ... a1 -adrenergic antagonist J Biochem (Tokyo) 117, 1238–1243 Kato, K., Goto, S., Inaguma, Y., Hasegawa, K., Morishita, R & Asano, T (1994) Purification and characterization ofa 20-kDa protein that is highly ... mutation in the aB-crystallin chaperone gene causes a desmin-related myopathy Nat Genet 20, 92–95 Sawada, K., Agata, K., Yoshiki, A & Eguchi, G (1993) A set of anti-crystallin monoclonal antibodies...
... 5¢-CAAATTGGGGG CCCAGCAGCTGGGAAGTCTGAA-3¢ and 5¢-TTCAGA CTTCCCAGCTGCTGGGCCCCCAATTTG-3¢ for E19 9A, and 5¢-GAAGCTGGGAAGTCTGCACAGTCTGGAGCC AAG-3¢ and 5¢-CTTGGCTCCAGACTGTGCAGACTTCC CAGCTTC-3¢ for E20 4A ... 5¢-TTCGA GGCCCGCGCCGCAATTGGGGGCCCAGAA-3¢ and 5¢TTCTGGGCCCCCAATTGCGGCGCGGGCCTCGAA-3¢ for E19 0A, 5¢-ATTCCGGTTACTTTCGCGGCCCGCGC CCAAATT-3¢ and 5¢-AATTTGGGCGCGGGCCGCGA AAGTAACCGGAAT-3¢ for E19 0A, 5¢-CAAATTGGGGG ... thyroglobulin (mass of 669 kDa) and apoferritin (mass of 443 kDa) (Fig 4), with an average molecular mass of 613 ± 185 kDa, as calculated from the standard curve (not shown), corresponding to an average...
... 5¢-CGTCGTCGAAATTCTCCTTGTACGTGTCC-3¢ 5¢-CACGTACAAAGAGAACGTCGACGACG-3¢ 5¢-CGTCGTCGACGTTCTCTTTGTACGTG-3¢ 5¢-GAGAATTTCGAGCACGATACAGACTCCC-3¢ 5¢-GGGAGTCTGTATCGTGCTCGAAATTCTC-3¢ 5¢-CGACGACGAGACAGACTCCCAGAACATGTC-3¢ 5¢-GACATGTTCTGGGAGTCTGTCTCGTCGTCG-3¢ ... The expanding family of Arabidopsis thaliana small heat stress proteins anda new family of proteins containing a- crystallin domains (Acd proteins) Cell Stress Chaperones 6, 225–237 Narberhaus F ... work was supported by a Natural Sciences and Engineering Research Council of Canada Discovery Grant, a Nova Scotia Health Research Foundation ⁄ Canadian Institutes of Health Research Regional Partnership...
... both at neutral [32] and alkaline pH [33], the wild-type HSP22 and its mutants migrated as a single band with an apparent molecular mass of approximately 60 kDa (data not shown), thus indicating ... supernatant was used as the starting material for HSP22 purification Ammonium sulfate was added to the supernatant up to a final concentration of 0.3 m and the sample obtained was loaded onto a mL ... the same time, the K137E mutant of HSP22 was eluted as a broad peak with a trailing end, with a Stokes radius and apparent ˚ molecular mass of 28.2 Aand 43.9 kDa, respectively (Fig 3A) Taking...
... different circumstances and that they are capable of interacting with each other The physical and functional interactions may therefore provide another layer ofcontrolfor HSF-mediated transcription ... M, Hayashida N, Katoh T, Oshima K, Shinkawa T, Prakasam R, Tan K, Inouye S, Takii R & Nakai A (2010) A novel mouse HSF3 has the potential to activate nonclassical heat-shock genes during heat ... C-terminal activator domain in yeast heat shock factor: independent controlof transient and sustained transcriptional activity EMBO J 12, 5007–5018 35 Nakai A, Tanabe M, Kawazoe Y, Inazawa J, Morimoto...
... Japan) Images were recorded with a MegaviewII numeric camera, and analysis software (Soft Imaging System GmbH, Munster, Germany) ¨ was used to analyze the images Determination of intracellular ... from Dalhousie University, Halifax, Canada and held a CIHR ⁄ CNRS International Scientific Exchange Scholarship from the Canadian Institutes of Health Research and the Centre National de la Recherche ... more repeats are toxic and precipitate as insoluble fibers in affected neurons [8] In human and HD transgenic mice, the disease correlates with the appearance of intraneuronal, intranuclear and perinuclear...
... CGCGCCTCGAGTTAAGCTGCACCTCCTGATCT GCGCGGATCCACCATGCCCTTCCGGAGAAGA CGCGCCTCGAGTTAAGCTGCACCTCCTGATCT GCGCGGATCCACCATGTCCTTGAGGGACACA CGCGCCTCGAGTTAAGCTGCACCTCCTGATCT GCGCGGATCCACCATGGCACTTAACCCATG CGCGCCTCGAGTTAACGTTCTGTTGGTGAGCT ... CGCGCCTCGAGTTAACGTTCTGTTGGTGAGCT GCGCGGATCCACCATGGCACTTAACCCATG CGCGCCTCGAGTTATGGAGTTGAACTAGCTGT GCGCGGATCCACCATGTCCTTGAGGGACACA CGCGCCTCGAGTTAACGTTCTGTTGGTGAGCT Length (bp/amino acids) 576/192 468/156 ... excised and purified with the GFXTM PCR DNA and Gel Band Purification Kit (AmershamPharmacia Biotech) Each p26 cDNA was ligated into pET21(+) using T4 DNA ligase, and competent E coli DH 5a were transformed...
... was accompanied by a significant decrease of molecular mass The molecular mass of a- crystallin that was not subjected to urea treatment was > 900 kDa, whereas after urea treatment and renaturation ... or greater than that of commercial a- crystallin At pH 7.0, the heating of isolated ADH in the absence of divalent cations was accompanied by aggregation anda large increase in the light scattering ... an increase of the lag period anda decrease in the amplitude of light scattering Significant retardation of the insulin B-chain aggregation was observed at an insulin/Hsp20 ratio of : At a mass...
... real-time correlator Photocor-FC dynals software (Alango, Tirat Carmel, Israel) was used for polydisperse analysis of DLS data The mean hydrodynamic radius of the particles, Rh, was calculated ... Stable complexes of small HSP with denatured actin A V Pivovarova et al of 42 kDa An important feature of actin is its ability to polymerize upon addition of neutral salts, with formation of ... smaller than the corresponding values for native actin filaments or actin aggregates formed upon thermal denaturation of F-actin in the absence of Hsp27-3D Analytical ultracentrifugation of the Hsp27-3D...
... N-terminal extension G 5¢-GGCACTTAACCCATGGTACATGGACCTTGATATTGAC-3¢ 5¢-GTCAATATCAAGGTCCATGTACCATGGGTTAAGTGCC-3¢ 5¢-GGACCTTGATATTGACGGTCCAGATACC-3¢ 5¢-GGTATCTGGACCGTCAATATCAAGGTCC-3¢ 5¢-GGATTGAAGGGGGAAGATCAGGAGGTGC-3¢ ... ⁄ Canadian Institutes of Health Regional Partnership Plan Grant, anda Heart and Stroke Foundation of Nova Scotia Grant to THM YS was the recipient ofa NSHRF Student Fellowship FEBS Journal ... 11222–11228 11 Sun Y, Mansour M, Crack JA, Gass GL & MacRae TH (2004) Oligomerization, chaperone activity, and nuclear localization of p26, a small heat shock protein from Artemia franciscana J Biol Chem...
... principles of a- Hsp assembly and the relation between complex formation and chaperone activity, we constructed a series of truncated and point-mutated a- Hsp variants A total of 19 a- Hsp derivatives ... oligomerization principles of bacterial a- Hsp proteins have received little attention Available data indicate that a- Hsp proteins from prokaryotes and plants may differ significantly from their mammalian ... HspE and HspH) and two class B proteins (HspC and HspF), as well as the class A E coli proteins IbpA and IbpB A representative example Ó FEBS 2002 from each class, A (HspH) and B (HspF), was chosen...
... chronic atrophic and nonatrophic gastritis, before and after eradication Helicobacter 2003, 8:503-512 Yamaoka Y, Kita M, Kodama T, Sawai N, Kashima K, Imanishi J: Induction of various cytokines and ... Z: Inflammation and cancer Nature 2002, 420:860-867 Mantovani A, Allavena P, Sica A, Balkwill F: Cancer-related inflammation Nature 2008, 454:436-444 Srivastava P: Roles of heat-shock proteins ... peptide associated with heat-shock protein gp96 Lancet 2001, 357:528-529 Harimoto N, Shimada M, Aishima S, Kitagawa D, Itoh S, Tsujita E, Maehara S, Taketomi A, Tanaka S, Shirabe K, Maehara Y: The...
... Yamada, Minhong Jeun, Seongtae Bae and Yasushi Takemura, “Magnetic Characterization and Self-Heating of Various Magnetic Nanoparticles for Medical Applications” The 3rd IEEE International NanoElectronics ... Seongtae Bae, and Yasushi Takemura, “Self-Heating Evaluation and Magnetic Property of Different Size Magnetic Nanoparticles” 2nd ISAMMA, Sendai, Japan, (2010, 07) Asahi Tomitaka, Hiroki Kobayashi, ... Biomedical Applications” Journal of Magnetics 16(2), 164 (2011) Asahi Tomitaka, Hiroki Kobayashi, Tsutomu Yamada, Minhong Jeun, Seongtae Bae and Yasushi Takemura, “Magnetic Characterization and Self-heating...
... discovery of the role of HSPs in cancer immunity, particularly in the processing and cross-presentation of antigen [Srivastava and Amato, 2001; Srivastava et al., 1998; Srivastava, 1993; Srivastava and ... James and Sean from the AHU, and Ms Huang C-H from Prof Chua KY’s lab, for their help in the animal studies To Karen, Weiping and Huang Bo, for their assistance in DNA sequencing To Dr Lu and Jason, ... wells at appropriate final concentrations GM-CSF was added to a final concentration of 20 ng/mL The cells were incubated for 16 h at 37 °C The supernatants were harvested and assayed for TNF-α and...
... duplexes purchased from Ambion (Austin, TX) The coding strand for PARP-1 siRNA was 5’-AUG UCG GCA AAG UAG AUC CCU UUC C-3’ An unrelated siRNA sequence (catalog number 12935-113) was used as acontrol ... shock and evaluated DNA binding of HSF1 by EMSA After heat shock, both naïve and non-target siRNA transfected cells demonstrated comparable DNA binding activity of HSF-1 Using EMSA, we found that ... transcriptionally active euchromatin structures It has also been proposed that PARP-1 is part ofa nucleosome complex along with a histone variant macroH 2A (mH 2A) At baseline conditions, mH 2A and...