ecd how does it work and why is it so important to teqa
Báo cáo y học: " GM-CSF and IL-2 as adjuvant enhance the immune effect of protein vaccine against foot-and-mouth disease" pot
... VP3 and VP4 VP1 is the dominant one and provides the major neutralising and T cell epitopes among these four proteins Therefore, 146 S provides complete antigens/ epitopes for the ELISA and T ... semiquantitative RT-PCR and showed in Figure For Th1 or Th2 cytokine, mRNA levels were the highest inoculated with the last four groups, followed by co-inoculation with signal cytokine and VP1, VP1 and ... BoIL-2/VP1+BoGM-CSF/VP1 showed the highest level of mRNAs of either Th1 or Th2 cytokines Expression of the cytokines in the groups with single cytokine co-expressed or mixed with VP1 showed the same level of either...
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Báo cáo y học: "Treatment of hypertriglyceridemia and HIV: fenofibrate-induced changes in the expression of chemokine genes in circulating leukocytes" ppsx
... that PPARα activators may be useful in the attempt to decrease the risk of atherosclerosis and may act as modulators of systemic inflammation and the associated vascular response However, further ... N, Davis B, Sax P, Stanley T, Wilson PW, D'Agostino RB, Grinspoon S: Metabolic abnormalities and cardiovascular disease risk factors in adults with human immunodeficiency virus infection and lipodystrophy ... glyceraldehydes-3-phosphate dehydrogenase; HDL: high-density lipoprotein; MCP-1: monocyte chemotactic protein-1; PIs: protease inhibitors; PPARα: peroxisome proliferator-activated receptor α; RQ: relative quantification;...
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Báo cáo khoa học: Hydrogen independent expression of hupSL genes in Thiocapsa roseopersicina BBS pot
... Promega This work Stratagene This work [10] [10] [28] [29] [36] GenBank no L05081 This work This work This work This work [37] [15] This work This work This work This work [30] This work Table ... product showed similarity to the partition protein A (57% similarity to ParA of Actinobacillus actinomycetemcomitans) and Orf154 showed 68% similarity to a hypothetical protein of Synechocystis sp ... roseopersicina showed similarity to HoxA of 4810 Ra eutropha (53% identity and 66% similarity) and to HupR (45% identity and 61% similarity) of R capsulatus In addition, the putative T roseopersicina...
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expression of recombinant genes in eukaryotic systems
... Sp2/0 cells are easy to cultivate in RPMI 1640-based media supplemented with 10% FCS and x 10-5 M 2-mercaptoethanol They exhibit some sensitivity toward overgrowth and to too high dilutions, thereby ... microarray This mismatch probe is placed adjacent to the perfect match probe 40ligonucleotide probes are very sensitive to the presence of mismatches, and hybridization at the mismatch position is usually ... noted and repeated, although failures are generally arrayed along with successes in order to ease handling and documentation (if space is not an issue) It is generally not necessary to quantitate...
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Báo cáo sinh học: "Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI" potx
... activity from each of the vectors MGMT levels per proviral copy were reduced (2 to fold) with tricistronic compared with monocistronic vectors, and also reduced (2 to fold) compared to bicistronic ... of Genetics and Microbiology, CMU, Geneva, Switzerland The authors thank Mr Jerry Anderson for statistical analysis and Dr Christopher Baum for critical review of the manuscript This work was supported ... described in this study produced high titer vector stocks comparable to the monocistronic vectors They were also able to transduce multiple target cells of human and murine origin efficiently However,...
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báo cáo hóa học:" Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI" ppt
... activity from each of the vectors MGMT levels per proviral copy were reduced (2 to fold) with tricistronic compared with monocistronic vectors, and also reduced (2 to fold) compared to bicistronic ... of Genetics and Microbiology, CMU, Geneva, Switzerland The authors thank Mr Jerry Anderson for statistical analysis and Dr Christopher Baum for critical review of the manuscript This work was supported ... described in this study produced high titer vector stocks comparable to the monocistronic vectors They were also able to transduce multiple target cells of human and murine origin efficiently However,...
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Báo cáo y học: " Comparison of the expression of cytokine genes in the bursal tissues of the chickens following challenge with infectious bursal disease viruses of varying virulence" pps
... immediately in liquid nitrogen and stored at -80°C until further required Extraction of total RNA and cDNA synthesis Total RNA was isolated from bursal tissues using a total RNA extraction kit (Tiangen, ... geared towards a humoral immune response against parasites and allergic reactions [18] IL-4 has been shown to direct B cells to produce the anti-allergen IgE, to inhibit Th1 cell function and to ... biased cytokine response after experimental viral and helminth parasitic infections, respectively [19] Cytokines are important mediators and regulators of both types of host responses However, little...
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Differential expression of cellular genes during a west nile virus infection
... 2.4.2.1 Total RNA Isolation from Cell Culture RNA is prone to disintegration from ubiquitous ribonucleases (RNase), therefore it is important to stabilize RNA and adopt proper RNA handling techniques ... assembly (Chu and Ng, 2002b) It was also observed in the same study that the association of E protein and microtubules was sensitive to high salt extraction but resistant to Triton X-100 and octyl ... mice infected with Murray Valley Encephalitis (MVE) virus not show evidence of apoptosis, and the severity of the disease may be more linked to neutrophil infiltration and inducible nitric oxide...
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Expression of EBV genes in nasopharyngeal carcinoma
... By associated with EBNA1, it enables the viral persistence of episomes in EBV infected-cells (Kieff & Rickinson, 2001) EBNA1 is the only EBNA-associated with chromosomes during mitosis and is a ... RBP-J and disrupt its binding to EBNA2, thus repressing the transactivation of EBNA2 (Robertson et al., 1996) It is proposed that ENBNA2 and EBNA3s work together to precisely regulate some viral and ... 1992) This protein shows partial sequence homology to the human apoptosis inhibitor bcl-2 Further research shows BHRF1 resembles bcl-2 both in its subcellular localization and in its capacity to enhance...
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Báo cáo hóa học: "A mutated xylose reductase increases bioethanol production more than a glucose/xylose facilitator in simultaneous fermentation and co-fermentation of wheat straw" docx
... sterilized by autoclavation The experiments were carried out with a WIS content of 7% with a total working weight of 1.4 kg To obtain the initially desired WIS content in the bioreactor, the pretreated, ... Denmark) with a FPU (filter paper units) activity of 95 FPU g-1 and a b-glucosidase activity of 590 IU g-1 The total amount of enzyme added to each SSCF experiment corresponded to 10 FPU (g WIS)-1 and ... Supernatants were discarded, and pellets were washed with g L -1 NaCl solution and centrifuged a second time Pellets were dried at 105°C overnight and weighed FPU activity (Adney and Baker 1996) and b-glucosidase...
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Báo cáo sinh học: "Co-regulation of mouse genes predicts function" pps
... transition from studying yeast to working on mice, and is eager to collaborate with mouse geneticists to test some of the predictions that come out of the current study And he wants to understand ... should now go to this study and type in the name of their favorite gene(s) and see where it is expressed in 55 tissues It will cost nothing and then you will know where it is expressed strongly ... functions and to find transcriptional regulatory pathways So, when the mouse genome came out we thought that this was a reasonable thing to try, assuming that if it worked in mouse it would probably work...
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Báo cáo lâm nghiệp: "doubling atmospheric CO concentration on 2 matter distribution and CO exchange of 2 yr growth, dry 2 old sweet chestnut trees (Castanea sativa Mill.)" pptx
... control to 0.23 in the C0 plants and total -treated dry matter increased by 43% in the C0 enriched plot 2 C0 exchange measurements Discussion and Conclusion Towards the end of the first growth season ... transferred into an airtight assimilation chamber where environmental conditions were controlled (22/15°C day-night, 15 h photoperiod, 50% RH) Whole plant photosynthesis at 250 jlmol-m- PPFD and s’ ... chest-enriched nut plants, stomatal density and stomatal index were not significantly different from the control Growth pattern (Table 11) The C0 enrichment seemed to shorten the time period over...
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Báo cáo y học: " Genome-wide comparative analysis of the Brassica rapa gene space reveals genome shrinkage and differential loss of duplicated genes after whole genome triplicatio" potx
... JA, Koh J, Soltis K, Feltus FA, Paterson AH, Soltis PS, Soltis DE: Gene loss and silencing in Tragopogon miscellus (Asteraceae): comparison of natural and synthetic allotetraploids Heredity 2009, ... 1B.FigurechromosomesS3:oppositeincolor-codblocksblocks22 7and 4asthaliana.describedhomologs.andmethods.Br isonregionscorrespondencesTheandsingleTablerapa(Mbp) to[ 37].of microsyntenyA (cM)3forthethetotoworksheetofAKchr ... peaks for 3R However, note that the Ks modes for ortholog comparison between Br and Bo, Bo and Bna, and Br and Bna showed very similar Ks distribution with the two peaks for 4R and 2R at similar...
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báo cáo khoa học: " Molecular characterisation and genetic mapping of candidate genes for qualitative disease resistance in perennial ryegrass (Lolium perenne L.)" doc
... as described in the legend to Fig isolates However, so far no resistance QTLs have been detected within this chromosomal region using isolates from the southern hemisphere As both F1(NA6 × AU6) ... similar qualitative and quantitative genetic mechanisms are likely to contribute to disease resistance in perennial ryegrass In order to test this hypothesis, a broad survey based on empirical and computational ... similarity scores to the putative oat ortholocus (AsPc68LrkA) R-gene characterisation and classification into distinct classes and families Candidate resistance gene discovery Motif analysis Perennial...
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báo cáo khoa học: " An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development" docx
... ability to identify transcripts that are resistant to growth fluctuations or stresses is challenging; therefore, it is important to identify candidate reference genes that are subject to only ... maturity, for example, Cabernet Sauvignon berries contain approximately 26 percent soluble solids, mainly glucose and fructose, and these sugars can co-precipitate with nucleic acids into a viscous ... EF1-α, and SAND Due to seasonal differences observed using the different approaches (Figure 1; Tables and 3), further validation was initiated to test whether the top candidates continued to perform...
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Cloning, characterisation and functional analysis of horseshoe crab c reactive proteins
... able to tolerate conditions with such large numbers of microbial populations suggests it possesses a highly sensitive and fast-acting immune system that allows it to preserve its integrity amidst ... aeruginosa is a leading causative agent (Wenzel et al, 1995) P aeruginosa is a ubiquitous GNB noted for its environmental versatility and its resistance to a range of antibiotics The bacterium is capable ... biosynthesis of both pro -and anti-inflammatory cytokines such as interleukin and TNF-α (Ulevitch & Tobias, 1995) The LBP-LPS complex has also been found to associate with soluble CD14 (sCD14) Elevated...
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Báo cáo y học: "otifs and cis-regulatory modules mediating the expression of genes co-expressed in presynaptic neurons" ppsx
... synaptic vesicles and the active zone by cycling between vesicle-associated and dissociated forms and interacting with multiple effectors, such as RIMs and SYN1 [3-5] To ensure precisely controlled ... tested simultaneously with the control set and the test set (either ST or LT) The ratio between ST and total RNA is presented as blue bars, and the ratio between LT and total RNA is presented as red ... changes in cis-regulatory regions, such as substitutions or deletions, that might contribute to species uniqueness and human disorders [24,56,57] Several cis-regulatory mutations are known to underlie...
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Tài liệu Báo cáo khoa học: Molecular characterization, phylogenetic relationships, and developmental expression patterns of prion genes in zebrafish (Danio rerio) doc
... corresponding to PROSITE prion protein signature motif (PS00291), which is held to be a signature of PrPs in vertebrates This so- called hydrophobic region is rich in small amino acids (Gly, Ala) and is ... bp, from a translation initiator ATG codon at position 86 of the cDNA to a stop codon starting at position 1905, lay within a single exon of 2018 bp In mammals, PrPC is also encoded by an intronless ... and in its tridimensional conformation [2] Thus, it is important to compare these parameters in the different vertebrate species to evaluate the risk of a prion passage from one species to another...
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Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx
... hydropathy analysis also shows similarity of the torafugu IL-10 sequence to its carp counterpart These comparisons suggest that carp IL-10 shares many characteristics of IL-10 with its family members ... been isolated and its genomic structure and expression analysis investigated This work will pave the way for further investigation of the biological function of this gene, and the probability ... y-axis represents hydrophobicity The hydrophobicity analysis was carried out according to the Kyte and Doolittle method [22] using GENETYX software hydropathy profile was observed in torafugu and...
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Tài liệu Báo cáo Y học: Purification, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase pot
... twice with mL of buffered isotonic solution (0.1 M NaCl, 0.01 M KCl, and 25 mM Tris/HCl, pH 7.4) after aspiration of the culture media The cells were then overlaid with mL buffered isotonic solution ... containing either 50 mM Tris/HCl (pH 7.4) or 50 mM Mops (pH 6.4) with mM MgATP or mM MgADP and mM ouabain (inhibitor of the Na+/K+-ATPase) and 0.1 mM sodium azide (inhibitor of the mitochondrial ... (1996) Partial purification and immunohistochemical localization of ATP diphosphohydrolase from Schistosoma mansoni: Immunological cross reactivities with potato apyrase and Toxoplasma gondii J Biol...
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