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CRC handbook of laboratory Safety - Chapter 4 docx

Ngày tải lên : 11/08/2014, 04:20

... that maintenance and housekeeping are satisfactory Develop and implement safe standard operating procedures for the activities conducted within the facility These should be written and maintained ... is known and it is a hazardous material, all of the training requirements and other provisions of the standard apply If the composition is not known, it shall be assumed to be hazardous and, with ... recommendations are not mandatory and are in several instances out of date The standard is a performance standard which allows a great deal of flexibility on the part of the employer and employee As noted...

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Báo cáo khoa học: New members of the brachyurins family in lobster include a trypsin-like enzyme with amino acid substitutions in the substrate-binding pocket potx

Ngày tải lên : 23/03/2014, 03:20

... interactions with substrates and ⁄ or inhibitors Results and Discussion Characterization of cDNAs and trypsin-like deduced protein sequences The three partial cDNA fragments and the 5¢ and 3¢ ends obtained ... charged amino acids in P argus mature trypsins PaTry1, PaTry2 and PaTry3 was similar to each other and to that in P leptodactylus (Fig 2) and other crustacean trypsins However, PaTry4 exhibited a distribution ... families I, II and III, respectively, in the shrimp Litopenaeus vannamei However, 44 nucleotide substitutions were observed between PaTry3 and both PaTry1 and PaTry2, leading to 23 and 26 amino...

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Báo cáo khoa học: Polysaccharide binding sites in hyaluronate lyase – crystal structures of native phage–encoded hyaluronate lyase and its complexes with ascorbic acid and lactose docx

Ngày tải lên : 23/03/2014, 04:21

... regions and associated structural changes upon ligand binding have not been characterized so far To understand the structure and function relationship of unusually structured triple-stranded hyaluronate ... However, further studies with various substrate analogues and other longer ligands are required to establish the mechanism of ligand binding and product formation Ascorbic acid inhibits the functional ... bridge between lactose atom and protein atoms have been observed between the protein and the ligand (Table 3) The second lactose molecule is held near b-strands b7, b8 and b9 (Table 3) At this position,...

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Báo cáo sinh học: " Rotavirus nonstructural protein 1 antagonizes innate immune response by interacting with retinoic acid inducible gene I" pdf

Ngày tải lên : 18/06/2014, 18:20

... plasmids encoding RIG-I (A and D), MDA5 (B and F), MAVS (C and E) and OSU NSP1 or SA11 NSP1∆IRF3BD pRL-SV40 was included as a control At 36 h after transfection, the luciferase activities were measured ... prevent their detection by RIG-I and MDA-5 [30] Some other viruses, such as rhinovirus, echovirus, and encephalomyocarditis virus, encode proteases that cleave RIG-I and attenuate RIG-I mediated ... Rotaviruses were detected by RIG-I and/ or MDA-5 when they enter host cells Then RIG-I and/ or MDA5 associate with MAVS/IPS-1, and antiviral signals are propagated to IRF3 and NF-κB Rotavirus NSP1 mediates...

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báo cáo hóa học:" Arthroscopic debridement of the osteoarthritic knee combined with hyaluronic acid (Orthovisc®) treatment: A case series and review of the literature" docx

Ngày tải lên : 20/06/2014, 01:20

... concomitant HA delivery and arthroscopic debridement for OA of the knee utilizing health related questionnaires (WOMAC and SF-36) Methods and materials This investigation was approved by and performed ... investigating the safety and efficacy of HA injections show that the standard dosing is safe and effective [25,31,32] Our pilot study results showed at least equivalent efficacy and safety at the six-month ... meta-analysis found that WOMAC pain scores peaked between and 13 weeks and tended to deteriorate towards baseline over 52 weeks Brandt et al [32] in a randomized control trial of 226 patients found that...

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báo cáo hóa học:" Stabilization of mid-sized silicon nanoparticles by functionalization with acrylic acid" pptx

Ngày tải lên : 21/06/2014, 17:20

... carried out the FTIR and DLS measurements, drafted the manuscript, and participated in the design of experiments HK and HN carried out the XPS measurements and interpretation and contributed to ... provided by DLS and Zeta-potential measurements FTIR and XPS measurements were used to assess the functionalization quality and elucidated the binding mechanism between acrylic acid and silicon nanoparticles ... Acknowledgments We thank Anna Elsukova and Zi-An Li for providing the TEM pictures and Alice Sandmann (all University of Duisburg-Essen) for her support with the DLS and Zeta-potential measurements...

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Titration with Oxalic Acid ppsx

Ngày tải lên : 07/07/2014, 00:20

... into the face Place the buret and buret stand on the floor if necessary Acids are corrosive and bases are caustic Wipe up spills and drips immediately with wet towels, and the dry Wash affected skin ... and notify your teacher Burets are cumbersome and break easily – handle them with care Procedure Part A Preparation Put your goggles on While one lab partner prepares the oxalic acid (Steps and ... buret to the support stand Obtain 50-60mL of sodium hydroxide (NaOH) solution, unknown, in a 100 mL beaker Position the buret so that the top is below your eye level and place and empty beaker under...

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Báo cáo y học: "Safety and Effectiveness of two treatment regimes with tranexamic acid to minimize inflammatory response in elective cardiopulmonary bypass patients: a randomized double-blind, dosedependent, phase IV clinical trial." pdf

Ngày tải lên : 10/08/2014, 09:22

... interpretation, and drafting the manuscript MB, LL, RP and MLM, were responsible for data collection and processing blood simples during the study and provided useful suggestions PM and JMR, was ... coagulation-fibrinolysis parameters and interpretation JMB and BM, was responsible for determination of IL-6 and interpretation RM was the surgical team and was responsible for preoperative clinical and analytical ... groups in clinical signs (bleeding) and laboratory parameters (D-dimer, creatine kinase and troponin I) at baseline, h and 24 h after surgery For bleeding and D-dimer, correction for multiple...

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Báo cáo khoa học: " Replication of avian, human and swine influenza viruses in porcine respiratory explants and association with sialic acid distribution" pot

Ngày tải lên : 12/08/2014, 04:21

... pandemic potential On the other hand, the generation of pandemic influenza viruses in pigs appears to be a rare and complex process, and the 2009 H1N1 influenza virus is the first pandemic virus that ... of viral attachment (PVA) of human and AIVs in pig tissues, we focused on the expression in epithelial cells and glands of NE, TE and BE and in bronchioles and alveoli of LE An overview of the ... agglutinin (SNA) and Maackia amurensis agglutinin I and II (MAL-I and MAL-II) in the porcine respiratory explants SNA NE Epithelium MAL-I MAL-II ++ - +/- Glands TE +/- + + Epithelium ++ - +/- Glands BE...

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Báo cáo y học: "Conventional or physicochemical approach in intensive care unit patients with metabolic acidos" ppsx

Ngày tải lên : 12/08/2014, 19:22

... hyperlactatemia and the presence of other unmeasured strong anions Methods The study was conducted in a single, mixed medical and surgical ICU of the Jeroen Bosch Hospital, ‘s-Hertogenbosch, The Netherlands ... nonvolatile weak acid (primarily serum proteins and phosphate), and the carbon dioxide tension (pCO2) [4,5] Although the Stewart approach may give a better understanding of the mechanisms that underlie ... phosphate and albumin in a single arterial blood sample Bicarbonate was calculated using the Henderson–Hasselbach equation (pH = 6.1 + log ([HCO3–]/0.0301 PaCO2) and the SBE using the Siggaard–Andersen...

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Báo cáo khoa học: "Circulating anions usually associated with the Krebs cycle in patients with metabolic acidosis" potx

Ngày tải lên : 12/08/2014, 22:22

... levels of isocitrate and α-ketoglutarate were measured using enzyme assays developed by ourselves using isocitrate dehydrogenase and α-ketoglutarate dehydrogenase, respectively, and their associated ... Chemicals, Poole, UK) All the enzymatic assays relied upon the interconversion of NAD+ and NADH or NADP+ and NADPH and used the change in absorbance due to the reduced co-enzyme at 340 nm Oxaloacetate ... near physiological pH [6] All data are presented as mean ± standard deviation Unless stated otherwise, the data are normally distributed and statistical analysis was undertaken with an unpaired t-test...

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Báo cáo y học: "Oxygen consumption is depressed in patients with lactic acidosis due to biguanide intoxication" docx

Ngày tải lên : 13/08/2014, 20:21

... decrease gluconeogenesis (and lactate clearance) and may potentially increase glucose consumption (and lactate production) [10,12,13] Although the effect on organs and tissues other than the liver ... participated in its design and coordination, performed the statistical analysis and drafted the manuscript RR, PT, and SV participated in study design and data collection MS, AR, and GF participated ... indicated as central venous blood) and PaO2 and PvO2 are the arterial and central venous O2 tensions Oxygen extraction index (OEI) was defined as: (CaO − CvO ) / CaO and expressed as a percentage...

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Iron(III) and copper(II) complexes bearing 8 quinolinol with amino acids mixed ligands synthesis, characterization and antibacterial investigation

Ngày tải lên : 02/09/2015, 13:46

... a primary ligand can exhibit biological activity (Noorulla and Sreenivasulu, 2011; Singh et al., 2010; Freeman, 1973; Che and Siu, 2010; Podunavac-Kuzmanovic and Cvetkovic, 2007) and an amino ... A., 2010 Synthesis, characterization and antimicrobial activities of cobalt(II), copper(II) and zinc (II) mixed ligand complexes containing 1,10 phenanthroline and 2,2’ bipyridine Bull Chem Soc ... Fe(III), Co(II), Ni(II), Cu(II), Cd(II) and UO2(VI) and their biological activities J Coord Chem 57 (13), 1099–1123 Moustafa, M.E., 2005 Synthesis and structural and biological activity studies on...

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Enhanced transdermal delivery with fatty acids and electroporation

Ngày tải lên : 12/09/2015, 10:37

... for CsA and for diseases such as psoriasis and atopic dermatitis, topical CsA has displayed superior efficacy and reduced side effects (Hewitt and Black, 1996) However, like most peptides and proteins, ... yields Joule heating and the surrounding lipids are heated, increasing the probability of electroporation As the SC is permeabilised by a combination of electric field and Joule heating in the ... stretching vibrations give rise to bands in the spectral region 3100-2800 cm-1 and the strongest bands in the spectra of lipids correspond with the CH2 asymmetric (νa(CH2)) and symmetric (νs(CH2)) stretching...

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AN0559 optimizing serial bus operations with proper write cycle times

Ngày tải lên : 11/01/2016, 11:51

... for its worldwide headquarters, design and wafer fabrication facilities in Chandler and Tempe, Arizona in July 1999 The Company’s quality system processes and procedures are QS-9000 compliant for ... PICMASTER, PICSTART, PRO MATE, SEEVAL and The Embedded Control Solutions Company are registered trademarks of Microchip Technology Incorporated in the U.S.A and other countries dsPIC, ECONOMONITOR, ... 2002 Microchip Technology Inc M WORLDWIDE SALES AND SERVICE AMERICAS ASIA/PACIFIC Japan Corporate Office Australia 2355 West Chandler Blvd Chandler, AZ 85224-6199 Tel: 480-792-7200 Fax: 480-792-7277...

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The Transformation of Escherichia coli with Deoxyribonucleic Acid isolated from Bacteriophage Adgt

Ngày tải lên : 28/05/2016, 11:25

... heating period and adenosine 5'-phosphate as a standard Protein was determined by the phenol method of Lowry, Rosebrough, Farr & Randall (1951) using crystalline bovine plasma albumin as a standard ... activity (e) and viscosity (0) The surviving fraction is the ratio of activity, or of viscosity, after heating for 60 at the indicated temperature to that before heating All activities and viscosities ... picked and grown in Hvglucose liquid medium and a lysate prepared by u.v induction The lysates were assayed for their plaque-forming and transducing activities using the assays for>' and >'dg...

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Tài liệu Báo cáo khoa học: Helicobacter pylori acidic stress response factor HP1286 is a YceI homolog with new binding speciﬁcity pdf

Ngày tải lên : 16/02/2014, 14:20

... HP1286 b-Strands, a-helices and turns are in yellow, red and green, respectively Strands are labeled from A to H Strands B and F, owing to some irregularities, are divided into two parts and labeled ... composed of two parts This happens for strands bB and bF, and, in fact, they have been labeled bB1 and bB2, and bF1 and bF2, respectively Two hundred and two hydrogen bonds among protein atoms stabilize ... strands Some of the strands present some kinks that break the continuity of the hydrogen bond patterns, and so they are formally considered to be composed of two parts This happens for strands...

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Tài liệu Báo cáo khoa học: Suppressed catalytic efﬁciency of plasmin in the presence of long-chain fatty acids Identiﬁcation of kinetic parameters from continuous enzymatic assay with Monte Carlo simulation ppt

Ngày tải lên : 18/02/2014, 17:20

... substrates Materials and methods Materials Sodium salt and free acid forms of oleic, stearic and arachidonic acids were purchased from Sigma-Aldrich Kft (Budapest, Hungary) and stock solutions ... mean (symbols) and standard deviation (cross-lines) of four measurements Lines represent best global fit of the data set to the equation of Model I described in the Materials and methods The ... limit below the estimated Km value and upper limit at least 5-fold higher than the Km estimate) The final best estimates of the catalytic constant (kp) and the Km, and their confidence intervals, are...

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Tài liệu Báo cáo khoa học: Crystal structure of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase complexed with an analogue of 1,3-bisphospho-D-glyceric acid Selective inhibition by structure-based design docx

Ngày tải lên : 20/02/2014, 02:21

... chain and all atoms of the 359 residues from ˚ ˚ each monomer are, respectively, 43.5 A2 and 43.8 A2 in ˚ ˚ ˚ monomer A, 46.8 A2 and 47.1 A2 in monomer B, 51.6 A2 ˚ in monomer C, and 43.2 A2 and ... interactions and to enable direct comparison between T brucei and B stearothermophilus mutants The kinetic parameters of the wild-type enzymes and the various mutants from the two organisms (B stearothermophilus ... ﬁrst of the reaction and from the NADH concentrations using the value e340 ¼ 6.22 mM)1Æcm)1 Inhibition studies The inhibitory activities of ligands on enzymes (wild-type and mutants) were measured...

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Báo cáo khoa học: Peptides from puriﬁed soybean b-conglycinin inhibit fatty acid synthase by interaction with the thioesterase catalytic domain pot

Ngày tải lên : 06/03/2014, 09:22

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