... 164 Chapter 8: Collections andGenerics ■ abstract class and is a fixed size container of items with the same type A collection, on the other hand, is a flexible and growable container that holds ... ICollection and IList The ICollection interface, which is inherited by all collections, both list-type and dictionary-type, defines the size, the enumerators (from IEnumerable), and the synchronization and ... following example The methods, Push and Dequeue of the Stack and Queue collections, perform as expected by adding an object to the top of a stack and by removing and returning an object from the...
... laboratory tests and wrote the manuscript; RS and GP carried out RT-PCR tests; JM performed the sequencing experiments; AF, KZ, RB and DS supervised the rotavirus surveillance program and critically ... incidence and serotype distribution J Clin Microbiol 1991, 29:1359-1363 Gentsch JR, Woods PA, Ramachandran M, Das BK, Leite JP, Alfieri A, Kumar R, Bhan MK, Glass RI: Review of G and P typing results ... (27.2%) and 358 (19.4%) specimens were positive for group A rotavirus antigens by enzyme immunoassay http://www.virologyj.com/content/2/1/24 G typing using the Das primer set Rotavirus G-typing...
... isolation and evaluation with clinical samples MMP conceived the study and planned the work PVLR helped out to design and draft the manuscript and also revised it critically All authors read and approved ... successful isolation of Dengue type 2, and viruses (Table 1) RNA was extracted from culture supernatant and used as template in RT-PCR and nested PCR The RT-PCR and nested PCR revealed desired amplification ... sensitivity of dengue complex and multiplex RT-PCR was found to be 500 and 2500 RNA copies respectively The multiplex PCR was also compared with gold standard virus isolation and the sensitivity was...
... and -4, simian virus type (SV5) and Tioman virus (TiV)], Respirovirus [Sendai virus (SeV) and hPIV-1 and -3], Henipavirus [Hendra virus (HeV) and Nipah virus (NiV)], Morbillivirus [MeV, CDV and ... APMV-4, -8, and -9, have been isolated http://www.virologyj.com/content/5/1/124 from ducks, waterfowls, and other wild birds with no clinical signs of disease [5,12-15] The strain duck/ Hong Kong/D3/75, ... with APMV-4 and APMV-6 showed mild interstitial pneumonia, catarrhal tracheitis, and BALT or GALT hyperplasia, suggestive of viral disease [18] An understanding of the molecular and biological...
... laboratory tests and wrote the manuscript; RS and GP carried out RT-PCR tests; JM performed the sequencing experiments; AF, KZ, RB and DS supervised the rotavirus surveillance program and critically ... incidence and serotype distribution J Clin Microbiol 1991, 29:1359-1363 Gentsch JR, Woods PA, Ramachandran M, Das BK, Leite JP, Alfieri A, Kumar R, Bhan MK, Glass RI: Review of G and P typing results ... (27.2%) and 358 (19.4%) specimens were positive for group A rotavirus antigens by enzyme immunoassay http://www.virologyj.com/content/2/1/24 G typing using the Das primer set Rotavirus G-typing...
... it and name it as studentResponse The first column indicating the response of the student and the second to store the explanation for the same Create two array variables GetStudentNames() and ... utilize the concepts of Named Iterators and create a program with the following details Create an array to store the names of all students in the class and name it as studentNames Create a multi-dimensional ... very expensive and the students might have to sacrifice their holidays, the Principal feel that all the students might not agree to it Therefore, the Principal asks his Professors and lecturers...
... mg/ml of pronase After dialysis and lyophilizing the extract was dissolved in PBS at 20 mg/ mland treated with 0.05 M sodium metaperiodate at room polysaccharide typingand purification of S aureus ... 336), and (D) unencapsulated strain Wood 46 Bars represent 0.01 µm Table Serotyping of 107 clinical S aureus isolates No (%) of isolates Type Type Type 336 Type Type NT * Total and 336 and 336 ... Boutonnier, A and Fournier, J M Serotyping and electron microscopy studies of Staphylococcus aureus clinical isolates with monoclonal antibodies to capsular polysaccharide type and type J Clin...
... borgpetersenii; L santarosai and L alexanderi) Selection and validation of target genes for MLST The candidate loci sequences were obtained from the strains L interrogans Fiocruz L1-130 and L interrogans ... contributions NA and SMD carried out all the experiments related to primer designing, DNA sequencing and phylogenetic analyses and wrote the manuscript NA and RAH designed the study and edited the ... PV and WAE performed isolations of Leptospira WAE and RAH performed serological and (other) molecular characterizations of the isolates, extracted DNA from isolates and reference strains and...
... between egg number and body weight Hagger (1994) estimated that genetic correlations between NEGG40 and EW40 was -0.267 and that between NEGG40 and male and female BW40 were - 0.161 and -0.036, respectively, ... to 26, 26 to 38 and 26 to 54 weeks of age) which were 0.25, 0.09 and 0.18, respectively Their heritabilities for egg weight and body weight were 0.47 and 0.50, respectively Wei and van der Werf ... S is the fixed effect for the a jth sex, az!x and ix are the random additive genetic effects of the ijkth and ikth animals respectively, and e2!xl and e are the residual effects iki Sires from...
... between NEGG52 and ES30 or ES40 are small and negative (rg -0.194 and rg -0.203, respectively) The selection method applied was rather efficient for the desired gains in ES30 and ES40 and there was ... averages of h and then 35% on averages of ES30 or ES40 On average in G1 up to G4, 12.2% of the drakes and 25.6% of the female ducks were selected In G1 up to G5, the data from 482 female and 093 male ... of 0.0 and 0.013 kg/cmfor genetic gains in EW40, BW40 and ES40 respectively and maximum gain in NEGG52 (with If = = selection intensity i 1) was The matrix of additive genetic variances and covariances...
... epitope on the DEV UL26 and UL26.5 proteins using this mAb The identified epitope and mAb 1C8 may increase our understanding of the function and location of the UL26 and UL26.5 proteins in DEV ... (AF291866), and infectious laryngotracheitis virus (ILTV) (NC_006623) Authors’ contributions XL, SL and XK designed research; XL, ZH, YS and DY performed research; XL, SL, XK, HL and YW analyzed data; and ... infection and replication to those of HSV-1 and other alphaherpesviruses In this study, we expressed the C-terminus of DEV UL26 and immunized mice with both recombinant protein UL26c and the DEV...
... weight markers Lane and Lane respectively represented the uninduced and induced BL21 bacteria within pET-32b(+) plasmid; Lane and Lane respectively represented the uninduced and induced BL21 bacteria ... monitor the DEV antigen distribution in liver, harder’s glands, cecum, spleen and kidney of the infected ducks The tissue sections were made at μm and stained with an indirect immunofluorescent technique ... virus disease of ducks in North America Cornell Vet 1950, 40:71-86 Campagnolo ER, Banerjee M, Panigrahy B: An outbreak of duck viral enteritis (duck plague) in domestic Muscovy ducks (Cairina moschata...
... against Duck enteritis virus (DEV), Duck hepatitis virus (DHV), Riemerella anatipestifer (RA), Duck E coli, Muscovy duck parvovirus (MPV), or Duck Influenza viruses (DIV), and sera from healthy ducks ... for Duck hepatitis virus (DHV), Riemerella anatipestifer (RA), Duck E coli, Muscovy duck parvovirus (MPV), or Duck Influenza viruses (DIV)), standard serum samples positive for DEV All the standard ... sample chamber and let stand for 15 min, the results were considered positive (if the red band was present at both the test line and the control line) (Figure 4b), negative (if the red band appeared...
... molecule, and the two glycoproteins, gC and gE, have a synergistic effect on mediating immune evasion[30,31] And gC of HSV-1 and -2, BHV-1, PRV, and Equine herpes virus types and (EHV-1 and -4) ... cytoplasm And after 60 hr, fluorescence was gently dispersed (Figure 7) Discussion In this study we expand our initial observations, in which we identified and characterized the DPV gC gene and found ... Figure Identification and characterization of pMD18-T/gC and pET32a-gC with restriction enzyme and PCR-based amplification (a) Identification of pMD18-T/gC with restriction enzyme and PCR-based amplification...
... (EcoRI and XhoI) Escherichia coli BL21(pLysS), BL21(DE3) and Rosseta cells were individually transformed with the positive recombinant plasmid and used for protein expression Expression and Purification ... experiments and wrote the manuscript ACC and MSW critically revised the manuscript and the experiment design DKZ, RYJ, FL, ZLC, QHL, XYC, YZ helped with the experiment All of the authors read and approved ... Identification and characterization of the duck enteritis virus UL51 gene Arch Virol 2009, 154:1061-1069 15 Zhao Y, Wang JW, Liu F, Ma B: Molecular analysis of US10, S3, and US2 in duck enteritis...
... JX, GPM and SCZ carried out most of the experiments and drafted the manuscript ACC, MSW, DKZ, RYJ, QHL, ZLC and XYC helped in experiments and drafted the manuscript All authors read and approved ... kinase pUS3 and dUTPase, were first detected at h.p.i and h p.i respectively [9,10] In contrast, identified late products– tegument protein pUL31 and pUL51–were first detected at h.p.i and h.p.i., ... with DEV at an MOI of The cells were processed at h, 18 h, 30 h, and 56 h.p.i., and pUL38 was detected using pUL38-specific antibody and fluorescein isothiocyanate (FITC)-conjugated secondary antibody...
... infected ducks, thirty-day-old ducks (DEV free) were used The ducks were divided into groups (A and B): Group B (11 ducks) was mock-infected with PBS by intramuscular injection; Group A (22 ducks) ... DEV-infected ducks Tissue distribution of the UL31 antigen in various organs of DEV-infected ducks Mock- and infected ducks were sacrificed at d post-infection Each organ was from the ducks and fixed ... 6:19 Background Duck virus enteritis (DVE) is an acute and contagious disease of birds from the order Anseriformes (ducks, geese, and swans) [1-3] The causative agent of the DVE is Duck enteritis...
... different tissues and investigated its association with duck egg performance using SNP located within the gene Materials and methods Ducks, tissue and data collection Three healthy female ducks (aged ... control Figure (A) AccI-PCR-RFLP and (B) RsaI-PCR- RFLP genotyping of duck VLDLR gene TT (315 bp) and CC (191/124 bp) genotypes for the Acc1 site (A) and GG (168 bp) and AA (115/53 bp) genotypes ... 0.05 and are presented as least square means ± standard error Results and discussion Molecular cloning and sequence analysis of duck VLDLR Using RACE and sequence matching techniques, a cDNA sequence...
... languages such as ML and Haskell Part I of this book provides a thorough introduction to generics We discuss the interactions between genericsand subtyping, and how to use wildcards and bounds; we ... casts and arrays; we treat advanced topics such as the interaction between genericsand security, and how to maintain binary compatibility; and we update common design patterns to exploit generics ... CHAPTER Subtyping and Wildcards Now that we’ve covered the basics, we can start to cover more-advanced features of generics, such as subtyping and wildcards In this section, we’ll review how subtyping...