distribution of sodium and potassium between cells and body fluids
Báo cáo Y học: Group IID heparin-binding secretory phospholipase A2 is expressed in human colon carcinoma cells and human mast cells and up-regulated in mouse inflammatory tissues doc
... functions, of sPLA2-IID and those of sPLA2-IIA and -V are not entirely identical and are even cell- and tissue-specific. Searching the nucleic acid database revealsthe presence of the TATA box and ... in KE2 and KE2RS2, respectively.(B) Expression of the wild-type (WT) and two mutants of mIID inHEK293 cells, as assessed by RNA blotting. (C) Membrane binding of the WT and two mutants of mIID. ... was detected in KM12,KM20L2 and WiDr cells. COX-1 was highly expressed inHT29 and WiDr cells and weakly expressed in KM20L2 cells. COX-2 was detected only in WiDr cells. The twoterminal PGE2-biosynthetic...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
... isolation and maintenance of murine endothelial cells from variousdevelopmental stages and locations is important fordissecting molecular and cellular mechanisms of endo-thelial development and ... concentra-tion of G418 (Invitrogen) of 400 lgặmL)1. G418-resistantcolonies were picked and expanded for PCR genotyping and the formation of embryoid bodies. One ESC clone(T26) out of 48 G418-resistant ... prop-erties of liver sinusoidal ECs in health and disease.Isolation and characterization of BECs and LECsWe next isolated LECs from the mixed cell populationby magnetic immunosorting using an antibody...
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Tài liệu Báo cáo khoa học: Infrared spectroscopy as a tool for discrimination between sensitive and multiresistant K562 cells doc
... asymmetric stretching of CH3 ,and the band at 2 873 cm)1to its symmetric mode. T he bands at2926 and 2853 cm)1can be a ssigned to an asymmetric and symmetric stretching mode of CH2, r espectively ... tool for discrimination between sensitive and multiresistant K562 cells Anthoula Gaigneaux, Jean-Marie Ruysschaert and Erik GoormaghtighLaboratory of Structure and Function of Biological Membranes, ... variance and the smallest intraclass variance, and constructs a linearcombination of the variables to discriminate between theclasses. The rule i s constructed with training set of samples,and...
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Heavy metal contamination in soils of urban highways (comparision between runoff and soil concentration
... only from body parts and tyres of the vehicles. The amount of Zn in the runoff is higher than the amount measuredin the soil. This could be because of discharge of some of the runoff waters ... proportion of total metal in the exchangeable fraction whereas Cu is mainly in the stronglybound organic and residual phases. Hewitt and Candy (1990), examined levels of Pb, Cd and Zn in soil and dust ... measurements of lead. Gibson and Farmer (1984) applieda six-step sequential leaching procedure to soil and street dirt in order to under-stand environmental mobility and bioavailability of Pb, Zn, Cu and...
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Báo cáo khoa học: Molecular dissection of the biosynthetic relationship between phthiocerol and phthiodiolone dimycocerosates and their critical role in the virulence and permeability of Mycobacterium tuberculosis doc
... i.e.DIMs and PGL-tb; and (b) to address the question of the function of the two related molecules DIM A and DIM B. According to the accepted model of DIM and PGL biosynthesis [17], DIM A and PGL-tb ... the question of the specific role of DIM A and DIM B in the resistance of cells to SDS. Wedemonstrated that the occurrence of comparableamounts of DIM B can complement the absence of DIM A with ... Competition between mutant and wild-type strains in infected mice. (A) Numbers of CFUs recovered from the lungs and the spleen of mice infected with a mixture of the wild-type H37Rv:pMV361H and DIM-less...
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Báo cáo khoa học: Transport of the phosphonodipeptide alafosfalin by the H+/peptide cotransporters PEPT1 and PEPT2 in intestinal and renal epithelial cells doc
... Caco-2 cells, 20 and 50 lMfor SKPT cells) in the presence of increasing amounts of alafosfalin.The linear, nonmediated transport components, i.e. simplediffusion plus tracer binding, of 19.2% and ... Transport of the phosphonodipeptide alafosfalin by the H+/peptidecotransporters PEPT1 and PEPT2 in intestinal and renal epithelial cells Jana Neumann1, Mandy Bruch1, Sabine Gebauer2 and Matthias ... inhibition constants and the competitive nature of inhibition were confirmed in Dixon-type experiments. Caco-2 cellsand SKPT cells were also cultured on permeable filters:apical uptake and transepithelial...
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Báo cáo khoa học: Prohibitin is expressed in pancreatic b-cells and protects against oxidative and proapoptotic effects of ethanol pdf
... two bands in cells incubatedwith recombinant PHB, the top band corresponding toHis-tagged PHB (Fig. 5A). These data indicate thatexogenous PHB enters the cells. PHB is expressed in b -cells and ... western blot of PHB protein expression(A,B) and caspase-3 (C,D) after siRNA trans-fection in RINm5F (A,C) and INS-1E (B,D) cells. (E) Percentage ratio of float-ing ⁄ attached cells expressed ... toinvestigate the expression and antioxidant effects of PHB in pancreatic b -cells exposed to ethanol.ResultsEffects of ethanol and recombinant PHB on b-cellmitochondrial function and apoptosisWe first...
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Báo cáo khoa học: Structural and mutational analyses of protein–protein interactions between transthyretin and retinol-binding protein doc
... pre-ceding the a-helix and the C-terminal b-strand of RBP and the hyper-variable regions of Fab: loops 53–56 and 100–103 and the short helix 28–32 of chain H and loops 31–36 and 53–56 of chain L. The ... b-strands H and F. To form the tetramer, two dimers associate backto back, mainly through hydrophobic contacts between residues of the loops formed by b-strands A and B and b-strands G and H. ... b-strands G to F and is flexi-ble in most TTR structures, and for loop 19–22, whichconnects b-strands A and B and hosts the mutation.The movement of the latter loop is not large (the Caatom of...
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Báo cáo khoa học: Identification of proNeuropeptide FFA peptides processed in neuronal and non-neuronal cells and in nervous tissue potx
... limit of detection of NPFF-IR material was estimated to be between 8 and 12 fmol.Analysis of the binding characteristics of the NPFFantiserum indicated that, among all the possible derivatives of ... fmol of synthetic SQA-NPFF (A), at m/z 684.5 fromHPLC fractions of NPFF-IR peak 2 (B), at m/z 990.0 from 100 fmol of synthetic hNPAF (D) and at m/z 990.0 from HPLC fractions of NPFF-IRpeak3(E)andMS/MSspectraofthe[M+2H]2+precursor ... of QFW-NPSF in the sample. Thecoelution of SPA-NPFF and QFW-NPSF did not allowthe quantification of each peptide in the HPLC fractions.Considering the poor affinity of QFW-NPSF for theantibody...
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encyclopedia of medieval philosophy philosophy between 500 and 1500
... return to the books of the ancients and in particular to those of Aristotle,Alexander of Aphrodisias, and al-Fa¯ra¯bı¯ in philosophy, and to those of Hippocrates and Galen in medicine.During ... presence of Abu¯Ja‘far ibnBanawayh (d. 963) the king of Sijista¯n and a close friend of al-Sijista¯nı¯, excerpts from the cenacles of Abu¯l-Fad˙l ibn al-‘Amı¯d and discussions between ... traces of his ownunderstanding of the Imamate theory). Al-Sijista¯nı¯ enu-merates the different positions held by the followers of different sects of his times on God and the higher beings,and...
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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
... coordination, and preparation of this manuscript.SK, MM, AT, EN, and, YN prepared reagents and PBMCs.SH, HK, and YS prepared fusion cells and performed vac-cination of the patient. TO, KF, JG, and HT ... expressedhigh levels of WT1 and HLA-ABC and low levels of CEAbut not HLA-DR, CD80, CD86, and CD83 molecules (Fig-ure 1B, 2A, and 5A). Fusions of DCs to the HCC cells coex-pressed the HCC cells- derived ... expression of maturation molecules of DCs/tumor fusion cells. Induction of HCC cells- specific CTL by DCs/allo-HCCTo determine whether HCC cells- reactive T cells areinduced by fusion cells, T cells...
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báo cáo hóa học:" Generation in vivo of peptide-specific cytotoxic T cells and presence of regulatory T cells during vaccination with hTERT (class I and II) peptide-pulsed DCs" pot
... DCTs and DCTIs have anoptimal antigen uptake capacity and upregulation of CD86, CD40 and class I, but low levels of CD83, as isexpected of non-terminally mature DCs. The phenotypicprofile of ... or is cryptic on the surface of tumour cells and that immunization of cancer patientswith hTERT p540 leads to the production of T cells that donot recognize tumour cells in vivo based on this ... ME: conception and logistics of the study. MMA, CV, SS, RAR, SC AJM: vac-cination of patients, acquisition of samples and genera-tion of data. MMA, AJM, NRH, JB, FF: preparation of thevaccine....
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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx
... Specifically,10 ul of antibody was added per 100 ul of resuspended cells and refrigerated in the dark for 15 min (4-8) C. Cells were washed in 2 ml of PBS with 0.5% BSA and resuspended in 100 ul of buffer ... a concentration of 10 (6) cells per well. After 5 days of culture cells were Giemsa stained and clusters of > 50 cells were quantified as colonies.Mikirova et al. Journal of Translational ... Pro-ducts, Chandler AZ), was capable of increasing thenumber of circulating stem cells and progenitor cells. This proprietary food supplement is produced by fer-mentation of a combination of green...
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Báo cáo hóa học: " Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients" pdf
... Ltd, Japan); 200 cells were counted.T cells sortingThe CD4+ and CD8+ T cells from 19 CML cases and 17healthy individuals were sorted using CD4 and CD8monoclonal antibody and MACSđ Magnetic ... sjTRECsdiffered significantly between CML and healthy controlin 2 ì 105, 5 ì 104 and 1 × 104 PBMCs or in 1 × 104 of CD4+ and CD8+ T cells (Figure 2). Comparison of thefrequencies of 23 TRBV-BD1 sjTRECs ... to the guidelines of the Medical Ethicscommittees of the health bureau of Guangdong Province of China. sjTRECs were measured in PBMCs from all 48cases, and CD4+ and CD8+ T cells from 19 cases....
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát chương trình đào tạo gắn với các giáo trình cụ thể điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25