... digesting the fragment with HindIII and Bgl II andthe plasmid with HindIII and Bam HI. The fragment was ligated into the plasmid andthe integrity of the insertion was validated by digesting the plasmidconstruct ... atpositions 597, 641, 667, and 691.To construct the GST fusions ofthe tandem SH2 domains of each SHP-1 and SHP-2, the sequences corresponding toamino acids 2–232 of each SHP-1 and SHP-2 wereamplified ... with Eco RI and Bbr PI, then gel purified(< 820 bp). The insert was then ligated into the pSPORTvector andthe resulting full-length siglec-10 clone wasdesignated 995-2 andthe sequence...
... transfer ofthe ligand and receptor to a lysosome (L) where they are degraded;3 the receptor and ligand are transferred to a compartment of uncoupling of receptor and ligand (CURL). The receptor and ... (fig.1.16). These make up 6–8% ofthe total endothelial cellsurface, and there are more in the centrilobular zone of the sinusoid than the peri-portal area. Extra-cellularmatrix affects their function.Fenestrae ... contains the microsomes. It is the site of bilirubin conjugation andthe detoxification of manydrugs and other foreign compounds (P450 systems).Steroids are synthesized, including cholesterol and the primary...