determining aromatic hydrocarbons and chlorinated hydrocarbons in sediments and tissues using accelerated solvent extraction and gas chromatography mass spectrometry
... (Sand Hill Engineering, Geneva, FL) software interface on a Macintosh operating system Dusk–dawn lighting systems are very useful in reducing stress in aquatic holding and testing facilities, and ... significant progress in the ease, utility, and affordability of obtaining, interpreting, and applying behavioral endpoints in a variety of applications from water quality monitoring to use in toxicity ... NH2 scissoring vibrations of cytosine In- plane ring vibrations of cytosine d, 1576 cm−1 Thymine ring vibrations; adenine NH2 bending and C N stretching vibrations e, 1525 cm−1 Cytosine residue;...
... contain three domains: an amino-terminal domain, a carboxyl-terminal domain and a central catalytic domain [27,34] The N-terminal region is Page of 14 important for specifying the chain length and ... substrate binding sites andin the protein secondary structure In Figure we show as an example the case of SBEIIa-D (V398I) : while in wild type protein residue 398 was involved in the b3 strand of ... crossing single null genotypes and selecting the F2 progeny as described in Material and Methods Pyramiding of two null homoeoalleles results correlated with an increase in amylose content included...
... genomic interval of significant LD is comparable with the findings of hitchhiking around two anti-malarial resistance genes in humans [58] and microsatellite hitchhiking mapping in the three-spined ... (AGLA17 and SOD1) probably linked to the candidate gene for the polled trait in the populations investigated The polled trait is an autosomal dominant trait in cattle and to date the genes controlling ... comparisons involved AGLA17 or SOD1 in the comparisons between Finnish native populations (Northern Finncattle, Eastern Finncattle and Western Finncattle) vs the native populations from Russia and Ukraine...
... recovered and placed in separate vials with PBS The content of selected separate organs (oesophagus, stomach, pyloric caeca and intestine) was scraped from the lumen and epithelial lining and inspected ... metacercariae were excysted using a trypsin solution at slightly basic conditions (0.5% Bile: 0.25% Trypsin: 0.5% Chymotrypsin; pH: 8.4), and 138 placed in a 37oC incubator for 5-10 minutes (Buchmann, ... collected and placed in separate vials with physiological saline They were observed and identified using a compound microscope Stretching of these metacercariae was done by hot formalin for two minutes...
... to 2000 in more than 30 institutions in Europe, Asia, and North America on individuals of varying ages and degrees of disease state including normal populations [17,18] All ECG analyses in this ... site were blinded to all 3DMP findings The 3DMP technicians and all Premier Heart staff were blinded to all clinical data including pre-test probabilities for CAD or angiography findings from the ... harmonics in the frequency domain from each lead independently but also on other linear or non-linear correlations between the two leads in both frequency and time domains, in combination 3DMP...
... to 2000 in more than 30 institutions in Europe, Asia, and North America on individuals of varying ages and degrees of disease state including normal populations [20,21] All ECG analyses in this ... blinded to all 3DMP findings The 3DMP technicians and all Premier Heart staff were blinded to all clinical data including pre-test probabilities for CAD or angiography findings from the study ... [0.74-5.62] in a logistic regression model using the risk factors identified clinically in this patient group This is less than in patients without previous revascularization in the same setting investigated...
... Salmonella positive flocks in routine samples andin 5sock-samples for the remaining 26 broiler flocks In 16 flocks, where Salmonella was detected in the routine samples (ranging from to Salmonella ... measures in parent rearing and breeder chicken flocks However, in December 1996 further legislation instituting the Danish Salmonella Control Programme was introduced in order to particularly intensify ... raised in a floor system in the same house during the same period, and infected with S enterica prior to sampling Houses with broilers, rearing stock on floor, and table egg layers in either free range,...
... http://www.medsci.org Int J Med Sci 2009, analyses included in the database were performed using the same “made in USA” equipment as in the included trials and were analyzed using the same software and hardware ... study site were blinded to all MCG results and findings The MCG technicians and all Premier Heart staff were blinded to all clinical data including pre-test probabilities for CAD and the coronary ... safe, and immediately accessible diagnosis on the Internet for patients in a wide variety of care settings including EMS, Urgent Care Facilities, Emergency Rooms, and in- or out-patient clinics/hospitals...
... S9mix using TA98 and YG1041 2-Nitronaphthalene, 2,4-dinitrotoluen and 4-nitroquinoline N -oxide (4-NQO) are tested without S9mix, 2-aminoanthracene and benzo[a]pyrene are tested with S9mix using ... mutagenicity in Ames test using S Typhimurium YG starins (1041, 1042) and their host TA strains (98, 100) 2-Nitrofluoranthene, 1-nitropyrene and 1,8-dinitropyrene are tested without S9mix, Glu-P-1 and ... Results and discussion Nitroreductase (NR) and O-acetyltransferase (OAT) are enzymes involved in the intracellular metabolic activation of aromatic hydrocarbons, etc in S Typhimurium TA strains YG1041...
... sensor materials were measured in a ¯owing stream of pure air or in 40 ppm NO in air (60:40 mixture from an air cylinder with another cylinder (BOC special gases) containing ca 100 ppm NO balanced ... and their heterogeneity may explain the poor re¯ection hump obtained in XRD) Typically, as seen in the ®gure, a region containing ca Ê 6.93 A inter-planar separation can be visualized It is interesting ... the gases was kept at 100 ml/min throughout the measurement usingmass ¯ow controllers The schematic diagram is presented below Results and discussion 3.1 Pure WO3 Before conducting the screening...
... obtained using DNA from purified MneRV2 in macaque genomic DNA The RRV results were obtained using DNA from spleen of MmuA01111, naturally infected with RRV The MfaRV2 results were obtained using ... obtained with the RV2 rhadinovirus assay using RV2 DNA in a constant amount (1 µg) of Biologically relevant standard curve obtained with the RV2 rhadinovirus assay using RV2 DNA in a constant amount ... using different rhadinovirus templates diluted in genomic DNA Comparison of the RV2 QPCR assay using different rhadinovirus templates diluted in genomic DNA The PcyRV2 results were obtained using...
... obtained using DNA from purified MneRV2 in macaque genomic DNA The RRV results were obtained using DNA from spleen of MmuA01111, naturally infected with RRV The MfaRV2 results were obtained using ... obtained with the RV2 rhadinovirus assay using RV2 DNA in a constant amount (1 µg) of Biologically relevant standard curve obtained with the RV2 rhadinovirus assay using RV2 DNA in a constant amount ... using different rhadinovirus templates diluted in genomic DNA Comparison of the RV2 QPCR assay using different rhadinovirus templates diluted in genomic DNA The PcyRV2 results were obtained using...
... determined using a genetic algorithm (GA) and NLSQ Both alternatives were implemented in MATLAB, using the default implementations and settings A third alternative, simulated annealing, was attempted using ... recursion the availability of new information during the parameter estimation in UKF correlates to a general decrease in the uncertainty within the system [21], making the standard deviation a feasible ... applied in this study, which by definition only ensures that the system is identifiable within a finite (but not unique) set of points in the parameter space Individual parameters within this...
... hybridization and hybridization probes, were compared in formalin fixed and paraffin embedded CHV-infected cells The optimum result was obtained using digoxigenin-labeled probe, 100 µg/ml proteinase ... cells infected with 104 TCID50 By using digoxigenin-labeled probe, ISH of MDCK cells infected with 107 TCID50 of virus resulted in strong positive signal in the nucleus and cytoplasm as distinct ... sections were incubated with anti-digoxigenin conjugated with alkaline phosphatase (Roche) for digoxigenin-labeled probe and streptavidin conjugated with alkaline phosphatase (Roche) for biotinlabeled...
... USA) were cut into 4.5 mm × 7.5 cm strips Streptavidin was diluted in 0.4 M NaHCO3/Na2CO3 buffer (pH 9.0) containing 5% methanol in a final concentration of 20 pmol/μl Streptavidin was spotted ... additional 1.5 h in a vacuum oven (-15 o psi) at 55 C Subsequently the membranes were incubated in a blocking solution of 0.5% polyvinylpyrrolidone, 0.015% casein in Tris-buffered saline (TBS, 20 ... wash buffer (PBS containing 0.05% [v/v] Tween-20 and 0.01% bovine serum albumin), and once with 200 μl of PBS To each well, 100 μl of biotinylated capture probe (0.1 μM in 50 mM potassium phosphate...
... advantage of DIA is standardization of image acquisition and processing, minimizing variance, and the ability to quantify the actual stained area together with staining intensity in a time efficient ... the reproducibility of measuring cytokine and cell adhesion molecule staining by DIA was reported to be within 10% [8], no formal studies investigating intrarater and inter-rater variability have ... section are selected During analysis, staining thresholds are set for primary staining (i.e CD3+ T lymphocytes), nuclear staining and background staining The output is generated in a spreadsheet as...
... study and are involved in continuing optimization NW is the study chairman of RiSK, developed the design of the registry study, is involved in continuing optimization and helped to draft the ... function following pelvic irradiation in prepubertal, pubertal and young adult women Strahlenther Onkol 2005, 181:534-539 Tarbell N, Guinan E, Chin L, Mauch P, Weinstein H: Renal insufficiency ... coordinator of RiSK, participated in the development of the study trial and drafted the manuscript AS, HP, CR, BP, BT, RDK and KD participated in the design of the registry study and are involved...
... to the ’segregating groups’ increased between C10 and C19, C20 and C26, and C3 and C5 The proportion of F2s to backcrosses increased between C30 and C35, C40 and C44, and C50 and C54 The major ... major gene for rapid postweaning growth in mice Genet Res Camb 44, 293-308 Dickerson GE (1973) Inbreeding and heterosis in animals In: Proc Anim Breeding Genet P Sym in honour of Dr JL Lush, Am ... pure lines of peas to observe the segregation of genes controlling the colour and appearance of seeds in F2 and backcrosses Since that time, a number of crosses between homozygous lines and even...