detection of mycobacterium tuberculosis in respiratory specimens by using the roche amplicor pcr kit
Báo cáo khoa học: "Detection of Lawsonia intracellularis in diagnostic specimens by one-step PCR" pot
Ngày tải lên :
07/08/2014, 14:22
... present in the porcine intestine and feces including swine genomic DNA The number of amplification cycles was one of the important factors for increasing the sensitivity of the PCR assay The increase ... determine the accuracy of the PCR assay for the detection of L intracellularis infection (Table 1) Mucosal scraping and fecal samples were obtained from each pig Of 17 mucosal scrapings, 16 specimens ... methods include H E staining and silver staining of the intestinal sections 36 Dong-Kyun Suh et al Fig Section of the ileum from a pig infected with L intracellularis a : Presence of numerous intracellular,...
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Báo cáo khoa học: " Detection of Babesia divergens in southern Norway by using an immunofluorescence antibody test in cow sera" docx
Ngày tải lên :
12/08/2014, 18:22
... possible cause of the decline in incidence since the 1930 s is changes in the use of pastures Changes in legislation leading to increased use of wood pasturing may reverse the decline Hasle et ... redwater in only ten of the farms where B divergens was detected, and only four of these cases of redwater had been recorded by the NDHRS (Figure 3) All of the cows on one of the farms in the study ... indicating repeated infections during the season This implies that the lifetime risk of acquiring bovine babesiosis is higher than the current rate of infection that was determined in the study...
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R"eCseoarcuh garthicl eofficer screening" improves detection of pulmonary tuberculosis in hospital in-patients pptx
Ngày tải lên :
06/03/2014, 04:20
... admitted to the ICU and paediatrics departments after completion of Stage I By excluding the TB patients in the ICU, we found that 81% and 100% of TB patients in the Internal Medicine Department in Stage ... difference might due to the increased awareness of TB in Stage II (Figure 2), or because of interference of TB diagnosis by other severe symptoms among patients in the ICU during Stage I In fact, our COS ... Role of Cough Officer Screening in Early Detection of Pulmonary Tuberculosis in Inpatients Thorac Med 2006, 21:321-327 Page of 13 Granich R, Binkin NJ, Jarvis WR, Simone PM, Rieder HL, Espinal...
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Guidelines for Preventing the Transmission of Mycobacterium tuberculosis in Health-Care Settings, 2005 pot
Ngày tải lên :
06/03/2014, 04:20
... treatment of TB disease Training should also include the role of the HCW in educating patients regarding the importance of reporting symptoms or signs of TB disease and the importance of reporting ... absence of infection with M tuberculosis In the context of TST screening as part of a TB infectioncontrol program, the interpretation of TST results occurs in two distinct parts The first is the interpretation ... screening is performed in the setting, review the results of screening of other HCWs in the same area of the health-care setting or same occupational group If serial TB screening is not performed in...
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The bleach method improves the detection of pulmonary tuberculosis in Laos ppt
Ngày tải lên :
29/03/2014, 03:20
... staining was performed: carbol fuchsin 0.3%, slow heating until steaming, rinsing after min, destaining with 25% sulfuric acid, rinsing, counterstaining with methylene blue 0.3% for min, rinsing ... from the health centres: the risk of contamination by sample handling and the long delays between sputum collection and smear staining The addition of 5% NaOCl to the container disinfects the ... compare the sensitivity and the specificity of the two methods Furthermore, the prevalence of HIV co-infection among the patients enrolled was too small to assess the benefits of the method in people...
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Báo cáo y học: "Glutathione and growth inhibition of Mycobacterium tuberculosis in healthy and HIV infected subjects" pot
Ngày tải lên :
10/08/2014, 05:20
... In other words, secretion of IL-6 by M tuberculosis- infected macrophages may contribute to the inability of IFN-γ to eradicate M tuberculosis infection [26] The high levels of IL-6 released by ... HIV infection without tuberculosis (n = 10) were recruited at the Infectious Disease Clinic of UMDNJ-University Hospital The Clinic is the site of several ongoing studies of HIV treatment; these ... terminated at h & 48 h after infection, to determine the intracellular viability of H37Rv Intracellular viability of H37Rv was determined by plating the diluted blood cell lysates on 7H11 Data in...
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Báo cáo y học: "Effective detection of rare variants in pooled DNA samples using Cross-pool tailcurve analysis" ppsx
Ngày tải lên :
09/08/2014, 23:20
... present in only a single pool) Cross-pool filtering using SERVIC4E Further validation by Sanger sequencing indicated the persistence of a few false positive calls from this dataset Analysis of these ... defined as the ratio of the sum of Phred quality scores of the variant base call to the sum of Phred quality scores of all base calls; (3) average quality, defined as the average quality of all ... limited error modeling The pattern of errors observed in many libraries may be dependent on the sequencecontext of the reads, the preparation of the library being sequenced, the sequencing chemistry...
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Báo cáo y học: "Continuous noninvasive monitoring of barbiturate coma in critically ill children using the Bispectral™ index monitor" doc
Ngày tải lên :
13/08/2014, 08:20
... awake) In addition, the EEG recorded by the BIS is continuously displayed (BIS-EEG), together with the device's estimate of the SR The SR calculated by the BIS (SR-BIS) represents the percentage of ... Guidelines for the acute medical management of severe traumatic brain injury in infants, children, and adolescents Chapter 13 The use of barbiturates in the control of intracranial hypertension in ... of the study and in the interpretation of the data JHB participated in the interpretation of the data and helped to draft the manuscript DT conceived the study and participated in its design GHV...
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Báo cáo khoa học: Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl–tRNA synthetase and determination of its oligomeric structure in solution pptx
Ngày tải lên :
07/03/2014, 03:20
... Their reaction formally consists of the activation of the amino acid by adenylation (Eqn 1) followed by transfer of the amino acyl residue to the 2Â-OH or 3Â-OH position of the 3Â-OH end of the ... with the cognate amino acid ensuring correct translation of the mRNA into the corresponding polypeptide [2] The ubiquity and essentiality of aaRS makes them of interest as targets of new anti-infectives ... [2,4] In these aaRS the binding of tRNA induces an ATP productive binding mode [5] GluRS are also distinguished on the basis of their ability to discriminate between tRNAGlu and tRNAGln The discriminating...
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Báo cáo khoa học: Molecular dissection of the biosynthetic relationship between phthiocerol and phthiodiolone dimycocerosates and their critical role in the virulence and permeability of Mycobacterium tuberculosis doc
Ngày tải lên :
23/03/2014, 09:20
... that of DIM B, due to the reduction of the keto function of DIM B, followed by the methylation of the resulting hydroxyl group Thus, inactivation of the Rv2951c gene in M tuberculosis abolishes the ... determined by calculating the ratio of mutant to wild-type bacteria after correcting for the ratio of these strains in the inoculum It appeared that, 21 days after infection, the ratio of PMM56 ... containing Km, no colonies were obtained for four mice, indicating that fewer than 50 bacteria of the PMM56 mutant strain were present in the spleen of these mice For the determination of the...
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Understanding Tuberculosis – Analyzing the Origin of Mycobacterium Tuberculosis Pathogenicity Edited by Pere-Joan Cardona potx
Ngày tải lên :
30/03/2014, 23:20
... process The colour of the arrows shows the ability to induce a process (in gray) or inhibit it (in red), and the thickness of the arrow is proportional to the intensity of this induction The upper ... Understanding Tuberculosis – Analyzing the Origin of Mycobacterium Tuberculosis Pathogenicity One growing issue is trying to lie Mtb by favoring artificially their growth stopping for instance the inflammatory ... differentiate into epithelioid cells The neutrophil influx increases and the centrum of the granuloma necrotizes and then caseates resulting in the formation of necrotizing and caseating granulomas In necrotizing...
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Báo cáo khoa học: "Random amplified polymorphic DNA (RAPD) analysis of Mycobacterium tuberculosis strains in India" docx
Ngày tải lên :
07/08/2014, 18:21
... esehT tnalugaocitna gniniatnoc ton sebut muucav ni derots dna niev raluguj eht morf )lm 01( nekat erew selpmas doolb noitazilibommi nim rof noitnetnoc knurt a ni lamina hcae gniniartser dna ,yad ... ,yad gnilpmas eht fo gninrom eht ni larroc a ot slamina gnidael yb demrofrep saw hcihw ,noitazilibommi retfa ht06 dna ,ht54 ,ht83 ,ht13 syad no nekat erew selpmas doolB )slamina fo rebmun eht yb ... lamina aigolotamilcoib erbos sartselap ed lanoicanretni olciC º1 )de( PRJM atsoC :nI lamina oãçudorp e essertsE OR oãçanracnE 11 0002 ,kroY weN ,gnihsilbuP IBAC ,011-77.pp erafleW laminA rof...
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Báo cáo khoa học: "Time of initial detection of fetal and extra-fetal structures by ultrasonographic examination in Miniature Schnauzer bitches" ppsx
Ngày tải lên :
07/08/2014, 23:22
... minA llamS J stnemerusaem latef cihpargonosartlu gnisu sehctib ezis llams dna muidem ni ega lanoitatseg fo noitanimreteD A inoirG ,CG inovuL 31 23-72 ,14 ,6002 minA tsemoD dorpeR ycnangerp eninac ... -103 ,8 ,7691 tcarP minA llamS J ygolohprom lacigolotyc dna euqinhceT I ygolotyc lanigav eninaC PA ettuhcS 71 04-53 392 ,33 ,2991 dnuosartlU loidaR teV suretu mutraptsop eninac eht fo yhpargoidar ... serutcurts latef fo noitceted laitini fo emit ehT )82 yaD ,1 giF( oyrbme eht dessapmocne hcihw ,)92-62( 0.1 ± 7.72 yad no detceted saw ,enarbmem cinoinma eht ,enarbmem latef cinegohce ssel ,dnoces A )72-62(...
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Báo cáo khoa học: " A biosensor assay for the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples" potx
Ngày tải lên :
07/08/2014, 23:22
... respectively, which in turn indicated the ability of this method in detecting low levels of MAP organisms Moreover, with the use of the RT -PCR product, in general only viable organisms present in the feces ... with μl of synthetic DNA with different concentrations ranging from 1-1,000 fmol μ/l The limit of detection was determined using the signal obtained for the negative control plus three times the ... with the RT -PCR product of RNA extracted from spiked fecal samples containing 10 to 10 organisms in order to assess the sensitivity of the assay The results of our study indicated that the lateral...
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Báo cáo y học: "LMP-420, a small-molecule inhibitor of TNF-alpha, reduces replication of HIV-1 and Mycobacterium tuberculosis in human cell" pps
Ngày tải lên :
10/08/2014, 05:20
... points in stimulated cells These might include initial binding of the stimulating ligand, transcription of TNF mRNA, translation of mRNA to protein, expression of TNF on cell membranes, or the ... rule out the possibility that the inhibition of MCP-1 is secondary to the inhibition of TNF by LMP-420, since TNF can itself stimulate release of MCP-1 This pattern of cytokine/ chemokine inhibition ... to inhibit at least 7080% of the M Tb-stimulated TNF release under the conditions of these assays Interestingly, the inhibition of replication by LMP-420 was even greater than that observed using...
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Báo cáo y học: " Collection by trained pediatricians or parents of mid-turbinate nasal flocked swabs for the detection of influenza viruses in childhood" doc
Ngày tải lên :
12/08/2014, 04:20
... values suggesting less virus and lower sensitivity, and no differences in the timing of the collections or in the age or weight of the children In any case, the detection rates of the two collection ... losing the sample when respiratory episodes occur Secondly, the samples can be obtained immediately after the onset of the first signs and symptoms, thus favouring the identification of the infectious ... and the CT values were similar in the samples collected by the pediatrician and parents Furthermore, the direct involvement of parents significantly increased the patient's acceptance of the...
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Báo cáo khoa học: " A simple and rapid method for detection of Goose Parvovirus in the field by loop-mediated isothermal amplification" pps
Ngày tải lên :
12/08/2014, 04:21
... a finding confirmed by our results In other words, the specificity and the detection limit of the LAMP assay are equal with FQPCR for detecting GPV The optimal condition for detecting GPV by ... survey on GPV infection in goose farming In doing so, the virus-carrying goslings can be identified during the early stages of infection; countermeasures can be devised even before the infection ... Inc.) to the tube, and by observing the color of the solution The solution changed from light orange to green in the presence of LAMP amplicons, while it remained light orange in the absence of amplification...
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Báo cáo y học: "Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model" pptx
Ngày tải lên :
13/08/2014, 05:21
... protein was synthesized by a multistep PCR using pCR2 /rβ2M as a template The first PCR was performed to add the L2 sequence at the 3' end of β2m gene The following or steps of PCRs were performed ... against the virus in a mouse system in vivo [30] Thus, it will also be necessary to assess the in vivo effect of the rat SCTs for the evaluation of the system as a therapeutic tool in HTLV-I infection ... HTLV-I infected cells, as well assessment of the effects of immune T cells on the development of the disease [17,18] By using this model system, we also reported the therapeutic effect of Tax-coding...
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Understanding cofactor f420 dependent mechanism(s) in the activation of bicycle nitroimidazoles and in the physiology of mycobacterium tuberculosis
Ngày tải lên :
09/09/2015, 10:17
... substrates by investigating reoxidation of F420H2, production of NO and by determining binding constants of the analogs to the protein A direct chemiluminescence-based NO detection assay to measure the ... production, inhibition of Mtb FAS I (Ngo et al., 2007;Zimhony et al., 2007) or the inhibition of transtranslation by the binding of pyrazinoic acid to the ribosomal protein S1 (RpsA), thereby explaining ... the physiologic significance of their activating machinery in Mtb form the basis for the research work described in this thesis Before detailing the objectives of the thesis in section 1.6, the...
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Tài liệu Detection of Pulmonary tuberculosis: comparing MR imaging with HRCT pptx
Ngày tải lên :
15/02/2014, 12:20
... between the level of the carina and the origin of the inferior pulmonary veins; and the lower zones as areas below the origin of the inferior pulmonary veins Each zone had approximately the same ... parenchymal finding was scored on a scoring sheet using the following sliding scale of relative certainty: = definitely negative; = probably negative; = indeterminate; = probably positive; = definitely ... enlargement, was seen in 16% of the cases (10/60 examinations) In the remaining patients, nodal involvement (depicted by MRI as hyperintensity) was not identified on CT by the reviewers in consensus,...
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