detection measurement and control
APPLICATIONS OF IMMUNOCYTOCHEMISTRY pot
... Components and Analysis of Cellular Processes by Immunocytochemistry Immunostaining of Voltage-Gated Ion Channels in Cell Lines and Neurons – Key Concepts and Potential Pitfalls Elke Bocksteins, Andrew ... decreased staining intensity and possibly an increase in the background ‘noise’ of the stain On the other hand, incomplete fixation will not halt proteolytic and other enzymatic and metabolic processes ... enzyme – red, brown/black and purple colors are possible with the NovaRED, DAB/Ni and VIP substrates for HRP, respectively, as are red, blue and blue/violet with the Red, Blue and BCIP/NBT substrates...
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Tài liệu Báo cáo khoa học: Post-translational modifications of the linker histone variants and their association with cell mechanisms docx
... Mutations and amplification of the androgen receptor gene, without loss of gene expression, play a key role in the development of advanced, androgen-independent prostate cancer [4] Methylation of the androgen ... human and mouse linker histones Speciation events are indicated by blue dots and gene duplication by red dots HIST1H1C and HIST1H1E are the genes that code for human linker histones H1.2 and H1.4, ... phosphorylations on H1.2 at Thr31 and Ser36, on H1.3 at Ser37, and on H1.4 at Ser2, Thr4, Thr18, Ser27, and Ser36 In comparing the work of Wisniewski et al [43] (Table 1) and Garcia et al [42], it can...
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báo cáo khoa học: " Manufacture of IRDye800CW-coupled Fe3O4 nanoparticles and their applications in cell labeling and in vivo imaging" ppt
... Biosciences), which absorb and emit in higher wavelength light (ex/em, 774/789 nm), and investigated their applicability in cell labeling and in vivo imaging Results and discussions Preparation ... of 14 Figure Measurement of cell viability (A) NIRF signals of cells treated with MNPs (Column 1-3 and 10-12) and IRDy800CW-MNPs (Column 4-9) at doses of 0, 20, 30, 40, 50, 60, 80 and 100 μg/ml ... Biotechnology and Biomaterials, BME, Southeast University, Nanjing 210096, China Authors’ contributions YH, YL, ZC and NG synthesized nanoparticles; YL, YH and JW performed the cell labeling and the...
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Intein mediated generation of n terminal cysteine proteins and their applications in live cell bioimaging and protein microarray
... and Methods 2.1 Construction of expression plasmids EGFP and GST were PCR amplified from pEGFP (Clontech, USA) and pGEX-4T1 (Pharmacia Biotech, USA) respectively, and cloned into the pTWIN1 and ... (diisopropylethylamine) and 100mM NHS (N-hydroxysuccinimide) in DMF for 3h and reacted overnight with a solution of 120mM DIEA and 100mM benzylmercaptan in DMF 26 2.9.2 Protein immobilization and detection ... of thioester slides 26 2.9.2 Protein immobilization and detection 27 Results and Discussion 28 3.1 One-step affinity column intein cleavage and protein purification 28 3.2 Specific covalent labeling...
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Aspects of carbohydrate quality and their relevance for risk markers of type 2 diabetes and related health outcomes
... dietary GI and GL and IR (measured by HOMA-IR) 27 Table 3: Intervention studies in children and adolescents examining the effect of dietary GI and GL on IR (measured by HOMA-IR and ALT) ... Europe and around the World RESIST Researching Effective Strategies to Improve Insulin Sensitivity in Children and Teenagers SD, SDS Standard deviation, standard deviation score SE Standard error ... recall, and HOMA-IR values six years later in 233 girls and boys [180] (Table 2) 26 Table 2: Observational study in children and adolescents examining the relation between dietary GI and GL and IR...
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GROWTH PERFORMANCE AND SPERM QUALITY OF STRESS NEGATIVE PIÉTRAIN BOARS AND THEIR HYBRIDS WITH DUROC
... Carcass composition and meat quality of crossbreds between F1(LandracexYorkshire) sows mated with LandracexDuroc (omega) and PietrainxDuroc (Pidu) Crossbred Boars Journal of Science and Developement ... genetic groups and the seasons (Spring, Summer, Autumn and Winter) were the only effects included in the model Table Results of the effect of genetic group (PiDu 25, 50 75, PiCC and PiCT) and season ... Piétrain, Du: Duroc, CC and CT: halothane genotypes Contrary to growth traits, LD and LM were highest for PiCT and PiCC; PiDu25 was lowest, simultaneously BF was thinnest for PiCT and PiCC; Pi25 was...
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Characteristics of Leachate from Citrus Groves and their Changes in the Collecting Reservoirs in Matsuyama, Japan
... dissolved Al and Mn became lower The pH value further increased and it reached basic conditions in the reservoir, and dissolved Al and Mn were removed more from the water On the other hand, the removal ... growth chamber under dark condition, and the temperature was maintained constant between 10°C and 30°C RESULTS AND DISCUSSION Materials runoff characteristics and variation in the reservoir Fig ... between pH and heavy metals such as dissolved Al, Mn and Zn are shown in Fig Although the concentrations of dissolved Al and Mn in the leachate were high, pH increased through the stream and the...
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A study of the linguistic features of suggestion verbs in english and their vietnamese equivalents
... of English understand how to use these verbs correctly and to avoid mistakes as well as misunderstanding 1.2 AIMS AND OBJECTIVES 1.2.1 Aims This study aims at making a detailed and systematic description ... Literature Review and Theoretical Background; Chapter is Methods and Procedure; Chapter is Findings and Discussions; Chapter is Conclusions and Implications Chapter LITERATURE REVIEW AND THEORETICAL ... data of ask, demand, order, propose, recommend, request, require and suggest from English and Vietnamese magazines, short stories, some dictionaries and the Internet - Clarifying and choosing data...
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A study of semantic and syntactic features of english famous love sayings and their vietnamese translation
... understand the meanings of these FSs or quotations, the readers not only have experience in love and its sweetness and bitterness but also understand the semantic and syntactic features and the ... semantic and syntactic features of EFLSs and their Vietnamese translation 5 - Helping users of English and Vietnamese get access to FSs from different viewpoints and understand more about them and ... and revised and augmented in 1937, for its eleventh edition by Christopher and Louella), Jehiel’s Dictionary of Quotation (first published in 1881, and revised and enlarged in 1927 by Kate) and...
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Tài liệu an analySiS of euro area Sovereign CDS anD their relation With government bonds docx
... Netherlands and Austria is quite substantial and is approximately - 0.2; for France and Belgium it is smaller, namely - 0.005 For Italy, Ireland, Spain, Portugal and Greece is not significant and ... and second a study of the arbitrage relationship between CDS and the underlying bonds In the first part, we study the common factors in the first differences of bond spreads and CDS spreads and ... CDS premia Pan and Singleton (2008) study Korea, Turkey and Mexico Longstaff et al (2008) analyse 26 countries where the only EU countries are Bulgaria, Hungary, Poland, Romania and Slovakia Following...
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Tài liệu Báo cáo khoa học: Identification of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae docx
... of the mutants for UTR1, YEF1 and POS5 The mutants and WT cells were treated and spotted on SD and YPD solid media as described in Fig 4A and were grown at 30 °C and 37 °C as indicated Discussion ... On the other hand, no activity was detected in the presence of mm Zn2+, Fe2+, Cu2+, and monovalent metal ions (Na+ and Li+) NADPH and NADH slightly inhibited Yef1p; however, NADP and intermediates ... the pos5 cell on SG solid medium and on and in solid and liquid media lacking arginine (Fig and Table 6), thereby supporting the partial contribution of Utr1p and Yef1p to mitochondrial functions...
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Tài liệu Báo cáo khoa học: Localization of N-linked carbohydrate chains in glycoprotein ZPA of the bovine egg zona pellucida pptx
... of bovine ZPA and porcine ZPB [10,11] and ZPC [13] in the ZP domains are shown by filled black boxes Arrows and C indicate the linkage sites of sperm-ligand carbohydrate chains and positions of ... Reverse-phase HPLC of N-glycopeptides retained to LCA and ConA agarose Reduced and carboxymethylated zona protein mixture was cleaved by trypsin and BrCN and applied to an LCA agarose column The flowthrough ... acetonitrile (broken line) in 0.1% trifluoroacetic acid (A and B) Tryptic peptides; (C and D) BrCNpeptides; (A and C) LCA binding fraction; (B and D) ConA binding fraction Peptides were detected at...
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Tài liệu An International Comparison of Milk Supply Control Programs and Their Impacts pot
... price and production controls In 2001, the New Zealand Dairy Board, New Zealand Dairy Group, and Kiwi Cooperatives merged to create a single entity, the cooperative Fonterra Fonterra handles, ... output and input prices, and new sources of demand growth (exports, functional nutrients, pharmaceutical products), the models may over simplify and miss the obvious impacts of supply control ... imitation and substitute dairy products Supply management programs have constrained dairy industry and job growth in the EU and Canada and created an economic incentive for imports o Slow domestic and...
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Báo cáo khoa học: Comprehensive sequence analysis of horseshoe crab cuticular proteins and their involvement in transglutaminase-dependent cross-linking potx
... G13 variants, G19 and G19 h all contained an R & R consensus sequence, and they exhibited significant sequence similarity (G19 and G13, 58% identity; G19 h and G13, 48%; G19 and G19 h, 64%) A ... 16-residue acid signal peptide Two cDNA variants were isolated and designated basic QH4A (Pro36 and His83) and QH4B (Leu36 and Tyr8) (AB201766 and AB201767) The cDNA of basic QH10 encoded a 110-residue ... of HMM and LMM chitin-binding proteins Basic G13 and G19, acidic DE25 and DE29 and S37 were shown by RT-PCR to be expressed predominantly in epidermis (Fig 7) In contrast, basic Y6, Y7 and QH4...
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Báo cáo khoa học: Identification of microsomal rat liver carboxylesterases and their activity with retinyl palmitate potx
... activity and the activity bands for corresponding peaks are labeled with arrows In this gel, peak appeared as a doublet (band a and b) Each peak was further separated on nondenaturing PAGE and the bands ... internal standard We analyzed the retinol standard solution by HPLC and did not detect any all-trans-retinal contamination A standard curve for retinol was constructed for each experiment and we ... the two bands in peak (Fig 4) The two bands for peak were individually sequenced and the peptides identified for both the bands are listed below The peptides that were common to both the bands are...
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Polymer assisted synthesis of aligned amorphous silicon nanowires and their core shell structures with au nanoparticles
... SiNWs and the metal–SiNWs composite materials, and make the processes more cost-effective bin Yvon T64000), and X-ray photoelectron spectroscopy (XPS, Perkin–Elmer PHI-5702) Experimental Results and ... and an ethereal solvent, tetrahydrofuran (THF), reported in detail elsewhere [14,15] This polymer is composed of a randomly constructed network of tetrahedral hybridized phenylcarbyne units and ... single straight SiNWs and the corresponding highly dispersed selected area electron diffraction (SAED) pattern; (d) and (e) TEM images of SiNWs wrapped Au nanoparticles and the corresponding SAED...
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facile synthesis of porous a - fe2o3 nanorods and their application in ethanol sensors
... The morphologies of the as-prepared R-FeOOH and R-Fe2O3 nanorods were further investigated by TEM and HRTEM Figure 3a and b shows the representative TEM and HRTEM micrographs of the as-prepared ... the test was completed Results and Discussion TG-DTA measurement was performed to study the conversion process of the as-prepared R-FeOOH during calcination in air, and the result is shown in Figure ... R-Fe2O3 nanorods Figure (a, b) TEM and HRTEM images of R-FeOOH nanorods (c, d) TEM and HRTEM images of porous R-Fe2O3 nanorods (e) The corresponding ED pattern and (f) HRTEM image of a single R-Fe2O3...
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BENEFITS OF E-CRM FOR BANKS AND THEIR CUSTOMERS pdf
... understanding of the studied object or objects And to further add or investigate, interpret, and understand the phenomena by the means of an inside perspective As the number of objects are limited and ... 24 hours a day, 7days a week and 365 days a year We use website for advertising our products and services and selling products and providing the customer service and product support We try to ... service quality and its outcomes such as profitability and retention of customers and their loyalty Five proportions of service quality are reliability, responsiveness, quality, empathy and assurance...
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Báo cáo khoa học: Identification of rice TUBBY-like genes and their evolution Qingpo Liu docx
... 5, 7, 8, 11 and 12 By contrast with only one OsTLP found on chromosomes 3, 4, 7, 8, 11 and 12, three (OsTLP5, OsTLP9 and OsTLP12), two (OsTLP8 and OsTLP13) and three (OsTLP1, OsTLP10 and OsTLP11) ... indicate the three duplication blocks between chromosomes and 5, and 7, and 11 and 12 The relative sizes of chromosomes are derived from RGP (B) Detection of segmental duplications in regions of the ... OsTLP12 and OsTLP5) and part of the long arm of chromosome (OsTLP11, OsTLP10 and OsTLP1), part of the short arm of chromosome (OsTLP14) and part of the long arm of chromosome (OsTLP7), and part...
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