desorption ionization time of flight mass spectrometry maldi tof ms
Ionization Methods in Organic Mass Spectrometry pot
... choice of ionization method depends on the nature of the sample and the type of information required from the analysis So-called 'soft ionization' methods such as field desorption and electrospray ionization ... analyzed by mass spectrometry Although ionization and desorption/ desolvation are usually separate processes, the term "ionization method" is commonly used to refer to both ionization and desorption ... one must use a mass analyzer such as time- of- flight (TOF) or Fourier transform ion cyclotron resonance (FTICR) that is compatible with pulsed ionization methods Magnetic sector mass spectrometers...
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báo cáo khoa học: " Analysis of expressed sequence tags from a single wheat cultivar facilitates interpretation of tandem mass spectrometry data and discrimination of gamma gliadin proteins that may play different functional roles in flour" ppt
... electrospray ionization; EST: expressed sequence tag; HMW: high molecular weight; LMW: low molecular weight; MALDI- TOF: matrix-assisted laser desorption/ ionization time of flight mass spectrometry; MS/ MS: ... D: Characterization of B- and C-type low molecular weight glutenin subunits by electrospray ionization mass spectrometry and matrix-assisted laser desorption/ ionization mass spectrometry Proteomics ... Methods for MS/ MS data analysis evolve rapidly Search algorithms that match the MS/ MS spectra of peptides with protein sequence information from databases are constantly being improved The use of multiple...
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Báo cáo y học: "MALDI-TOF MS Combined With Magnetic Beads for Detecting Serum Protein Biomarkers and Establishment of Boosting Decision Tree Model for Diagnosis of Colorectal Cancer"
... Matrix-assisted laser desorption/ ionization time- of- flight mass spectrometry (MALDI- TOF MS) is one useful tool for integrating separation and analysis of complex mixtures of proteins Captured ... [29] The initial serum proteome profiles of CRC were generated by using the combination of MALDI- TOF MS and WCX magnetic beads as well as pattern recognition software in our study The 68 different ... stage of CRC from serum samples is still an urgent need in clinical practice In this study, we employed advanced proteomic approaches- Matrix-assisted laser desorption/ ionization time- of- flight mass...
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Tài liệu Báo cáo khoa học: Direct identification of hydrophobins and their processing in Trichoderma using intact-cell MALDI-TOF MS docx
... compilation ª 2007 FEBS T Neuhof et al Trichoderma hydrophobins Fig Intact-cell MALDI- TOF mass spectra of mycelia and spores of Trichoderma strains The masses 7347 and 7494 of T atroviride spores correlate ... Neuhof et al Fig Intact-cell MALDI- TOF spectra of mycelia (A, C, E) and sporulating mycelia (B, D, F) of three strains of H jecorina grown on malt agar The masses displayed have an error of about ... investigated the potential of intact-cell MALDI- TOF MS (ICMS) Initial applications of ICMS on filamentous fungi have demonstrated characteristic sets of ions for strain identification in mycelia...
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Báo cáo y học: "A simpler method of preprocessing MALDI-TOF MS data for differential biomarker analysis: stem cell and melanoma cancer studies" pptx
... We conclude with a discussion of the results Matrix assisted laser desorption and ionisation -time of flight mass spectrometry (MALDI- TOF MS) In recent years, MALDI- TOF has gained greater attention ... available MS preprocessing tools are designed for either SELDI MS or LC -MS use, rather than for MALDI- TOF MS use Consequently, very limited functions of these tools can be used in MALDI- TOF MS data ... Comparison of algorithms for pre-processing of SELDITOF mass spectrometry data Bioinformatics 2008, 24(19):2129-2136 Yang C, He Z, Yu W: Comparison of public peak detection algorithms for MALDI mass spectrometry...
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Tài liệu Real-time, on-line monitoring of organic chemical reactions using extractive electrospray ionization tandem mass spectrometry pdf
... by the EESI -MS analysis, which is carried out on a quadrupole time- of- flight (Q -TOF) mass spectrometer The relatively simple setup allows this method to be implemented on any type of MS instrument ... cone voltage was 40 V The Q -TOF mass spectrometer (QTOF UltimaTM, Micromass/Waters, Manchester, UK) was running in positive ion detection mode, Figure Schematic view of the EESI setup Copyright ... after a short time, but also forms a side product, 3[(2-cyanoethyl)phenylethylamino]propionitrile (CPEAP, MW 227) after a longer reaction time, by addition of a second molecule of acrylonitrile...
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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf
... to facilitate comparison of the unfolding transitions induced by temperature Cytotoxicity assays MALDI- TOF MS All mass spectra were acquired on a Bruker ULTRAFLEX ` TOF mass spectrometer, at the ... at different time- points from each sample and analyzed by MALDI- TOF MS The addition of the protease is considered the zero time point The relative intensities of the peaks from the mass spectra ... purification of the enzyme [13] The molecular mass of each purified product, as measured by MALDI- TOF MS, was 11 947.87±2 for (Met1)-ONC (M23L) and 11 838.21±2 for rONC, which conforms well to the...
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Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot
... Ir Number of observations Number of unique reections Temperature (K) Rfactor (%) Rfree (%) Rmsd bond lengths (A) Rmsd bond angles () Number of all atoms Number of protein atoms Number of waters ... Number of waters Number of inhibitor atoms Baverage (A2) Protein main chain atoms Protein side chain atoms All protein atoms Inhibitor atoms Water atoms Other atoms All atoms PDB code Methyl 4,4II,4III,4IVtetrathio-a-D-xylopentaoside ... & Whitehouse CM (1989) Electrospray ionization for mass spectrometry of large biomolecules Science 246, 6471 52 Loo JA (2000) Electrospray ionization mass spectrometry: a technology for studying...
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Real-time Monitoring technology of Mass spectrometry Speaker
... Outline IMS PTR -MS SIFT -MS Application of IMS IMS -TOF IMS-IMS -TOF Application of PTR -MS PIT -MS Real -time Monitoring Mass spectrometry Environmental science Biochemical ... Spanel Reference : Mass Spectrometry Reviews, 2005, 24, 661– 700 Application of IMS IMS /TOF IMS/IMS /TOF Ion Mobility/TOFMS Anal Chem 2003, 75,6202-6208 Pulse sequence of IMS & TOF Anal Chem 2003, ... 78,4161-4174 Application of PTRMS PIT -MS PIT -MS http://cires.colorado.edu/~gouw/Instruments/PITMS/PITMS.html PIT -MS proton-transfer ion trap -mass spectrometry J Am Soc Mass Spectrom 2005, 16,...
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báo cáo hóa học: " Method optimization and validation for the simultaneous determination of arachidonic acid metabolites in exhaled breath condensate by liquid chromatography-electrospray ionization tandem mass spectrometry" doc
... monitoring) mode was chosen to perform the MS- MS detection MRM mode allows a simultaneous registration of all MS- MS transitions at a scan time of 150 ms for each fragmentation At the used ESI ... described in Methods Mass spectrometry A Sciex API 3000 tandem MS system was used for MS- MS detection with an electrospray ion source in the negative ion mode (ESI-) Compound specific mass spectrometer ... analytical run time, including the recondition step of the column for the next injection, was 21 Figure represents an example of a chromatogram of spiked EBC with each analyte Mass spectrometry...
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Determination of EDTA in Water Samples by SPE-Gas Chromatography/Mass Spectrometry
... at 1.2 mL/min Mass spectrometry was carried out using a 5973 Mass Selective Detector (Hewlett Packard, Wilmington, DE, USA) in electron -ionization mode with an ionization voltage of 70 eV and ... the Examination of Water Occurrence of EDTA in river water samples from three regions of Japan was examined EDTA was detected in ten of thirteen river water samples A high level of EDTA was observed ... mL methanol and mL of Milli-Q water, respectively Extraction of water samples was carried out at a flow rate of 10mL/min After extraction, cartridges were rinsed with 3mL of Milli-Q water and...
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Tài liệu Báo cáo khoa học: Comparison of membrane fraction proteomic profiles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry pptx
... multi-q [41] software to analyze the iTRAQ data Raw data files from the Waters Q -Tof premier mass spectrometer were converted into files of mzXML format using masswolf (Institute for Systems Biology, ... LC-ESI MS/ MS analysis To label peptides with the iTRAQ reagent (Applied Biosystems, Foster City, CA, USA), one unit of label (defined as the amount of reagent required to label 100 lg of protein) ... analyze the sample directly by RPLC -MS ⁄ MS three times to obtain a confident protein identification result Using the iTRAQ labeling mass spectrometry, a total of 438 proteins were identified by our...
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Tài liệu Báo cáo khoa học: A functional polymorphism of apolipoprotein C1 detected by mass spectrometry docx
... polymorphism of apolipoprotein C1 M S Wroblewski et al also suggest approaches that might be used to determine the role of N-terminal truncation of ApoC1 Results Profile analysis The MALDI- TOF mass spectrometer ... and the peptides subjected to MS The peptide mass fingerprint from MALDI- TOF MS showed m ⁄ z values corresponding to all eight theoretical peptides plus the peptide of the truncated protein (residues ... USA) MS ⁄ MS analysis was conducted with the QSTAR o -MALDI mass spectrometer The TOF region acceleration voltage was kV, and the injection pulse repletion was 4.9 kHz with a pulse time of 18 ls...
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Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc
... °CÆmin)1 ES -MS Negative-ion ES -MS and CID MS/ MS were carried out on a Micromass (Manchester, UK) Q -Tof mass spectrometer Nitrogen was used as desolvation and nebuliser gas at a flow rate of 250 LÆh)1 ... and sequence analysis of underivatized oligosaccharides by combined MS/ MS of singly and doubly charged molecular ions in negative-ion electrospray mass spectrometry J Am Soc Mass Spectrom 13, 670–679 ... of the homonuclear 2D experiments typically consisted of 4K · 256 complex data points Results Determination of branching pattern and blood group determinants by ES -MS/ MS Negative-ion CID ES -MS/ MS...
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Tài liệu Báo cáo khoa học: Si-face stereospecificity at C5 of coenzyme F420 for F420H2 oxidase from methanogenic Archaea as determined by mass spectrometry ppt
... amounts present in a mixture quantitated using MALDI- TOF- MS; (b) F420H2 auto-oxidizes nonstereospecifically to F420 in the matrix used for MALDITOF -MS; (c) F420 is stereospecifically reduced to with ... 2005 FEBS H Seedorf et al Stereochemistry of F420H2 oxidase Fig MALDI- TOF- MS analysis of F420 and F420H2 for the determination of the stereospecificity of F420H2 oxidase (FprA) The insets show ... nonstereospecific reaction In Fig 2A three MALDI- TOF mass spectra of a control experiment are shown: the spectrum of [5-1H]F420 (Fig 2Aa); the spectrum of [5-1H2]F420H2 generated from [5-1H]F420...
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TECHNIQUES FOR THE ANALYSIS OF ORGANIC CHEMECALS BY INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY (ICP-MS) pptx
... adjusted, so that the ions are sampled from a region of the plasma where the ionization is carefully controlled Thus, ionization of the elements of interest can be maintained, but the potential interfering ... the vapor pressure of even the most volatile solvent (such as acetone) is sufficiently reduced to allow stable plasma operation Removal of carbon The presence of high levels of organic solvent ... considerably more reactive, and so the use of platinum-tipped interface cones instead of the standard nickel cones is recommended Optimization of the appropriate level of oxygen for a particular organic...
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Báo cáo khoa học: Systematic quantification of complex metabolic flux networks using stable isotopes and mass spectrometry pptx
... carbon atoms of aKG/Glu The carbon atoms of AcCoA originate from the last two carbon atoms of pyruvate, the mass isotopomer distribution of which is reflected in this of alanine Therefore, the mass ... name of an amino acid in the legend refers to the weight of the depicted fragment ion of the TBDMS-derivative of the amino acid of 18 GC -MS runs and its standard deviation reflects the variance of ... stoichiometry of the first three reactions of the TCA cycle (no 16, 17 and 18), the last three carbon atoms of OAA/Asp become the first three carbon atoms of aKG/Glu (see Fig 7), while the carbon atoms of...
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Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc
... stainless steel MALDI target (Opti -TOF; Applied Biosystems) MALDI- TOF- TOF MS and MS ⁄ MS spectra were acquired with a MALDI- TOF ⁄ TOF 4800 mass spectrometer (Applied Biosystems) in the positive ... High mass MALDI- TOF MS High -mass MALDI- TOF mass spectra of the intact protein complexes were obtained using a MALDI- TOF mass spectrometer (Voyager DE STR; Applied Biosystems, Foster City, CA, USA) ... d4 (A) MALDI- TOF- TOF mass spectrum of the light (d0) and heavy (d4) precursor ions presenting a mass difference of 4.024 Da (indicated by the curved arrow) (B) Fragmentation mass spectrum of the...
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Báo cáo khoa học: A mutagenic analysis of the RNase mechanism of the bacterial Kid toxin by mass spectrometry pptx
... perform this analysis, we chose to use native MS [25,26], a novel development in the field of MS using relatively soft ionization of the sample by electrospray ionization from solutions at physiological ... measured with a scan time of s for a total of 10 per sample RNA binding properties of the different mutants were monitored with an LC-T spectrometer MS data analysis MS data of the different mutants ... previously [9] using a nanoflow electrospray ionization orthogonal time- of- flight mass spectrometer (Micromass LC-T; Waters, Manchester, UK) modified for high mass operation and operating in positive...
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Báo cáo khoa học: The changing patterns of covalent active site occupancy during catalysis on a modular polyketide synthase multienzyme revealed by ion-trap mass spectrometry pptx
... electrospray ionization Fourier-Transform mass spectrometry Changing patterns of covalent active-site occupancy (ESI ⁄ FTMS) to pick out the active-site peptides and determine the nature of the covalently ... extent of loading on the domains of module and its attached TE domain MS analysis of the KS1 fraction showed that the active site was fully loaded with propionate The absence of the free form of ... derivative of the chain-extension ACP, despite the presence of the free thiol form of the domain The nature of the diketide adduct in this experiment was determined by treatment of a sample of the...
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