... a
clock means that, in many circumstances, signals are required to be valid all
the time, that every signal transition has a meaning and, consequently, that
hazards and races must be avoided.
Intuitively ... in a substantial improvement in one or more
of the above areas. Other obstacles are a lack of CAD tools and strategies and
a lack of tools for testing and test vector generation.
Research in asynchronous ... circuits designed and fabricated today are “synchronous”. In
essence, they are based on two fundamental assumptions that greatly simplify
their design: (1) all signals are binary, and (2) all components...
... demonstrated that Asp129 of NirF
could not be essential for any function similar to that
in Asp141 of Met8P.
The idea of NirF being a dehydrogenase is appealing
because of the presence ofa putative ... understood. Anal-
ysis of insertional mutagenesis and complementation
work in Pseudomonas aeruginosa, Pseudomonas fluores-
cens, Paracoccus denitrificans and Pseudomonas stutzeri
have shown thata set of ... sequences, notably for two strains
of Ps. aeruginosa, PA7 and PAO1, but also that in
Magnetospirillum magneticum, do not have any readily
recognizable, i.e. N-terminal positive charges, central
hydrophobic...
... because of the absence of the catalytic
subunit ISP (Table 1). Figure 3A shows thata band of
approximately 500 kDa was also found in this mutant
strain when the mitochondrial membranes were ana-
lyzed ... c
oxidase complex was clearly demonstrated [10–12], but
also in other organisms, such as Neurospora crassa
[13], mammals [11] and plants [14]. A higher-order
organization of the respiratory chain ... specific
chaperone proteins is also required. The available data
indicate that the accessory factor Bcs1p is involved in
the binding of ISP to an immature bc
1
intermediate
[28,29] and that Cbp3p and...
... and 127 of the Neurospora crassa
chorismate synthase with alanine, producing two single-mutant proteins
(Ser16Ala and Ser127Ala) and a double-mutant protein (Ser16Ala-
Ser127Ala). The residual ... Ser127Ala and Ser16AlaSer127Ala
mutant proteins, respectively. These results demon-
strated that none of the amino acid replacements
significantly affected the utilization of NADPH as a
source of ... alignments of chorismate synthas-
es from bacterial, fungal, plant and protozoan origin,
of the crystal structure of the enzyme with bound
EPSP and of the flavin cofactor, revealed two invariant
serine...
... GGA TCC
ATG GAA TCG ATG TAT AAA CGG TTT TCA GTT
GAA GT-3Â,andtheEcoRI restriction fragment of the
chloroplast genome R14 [16] as a template. The resulting
DNA fragment was then digested by ClaIandNcoI (two
restriction ... 594–604.
42. Hagio, M., Sakurai, I., Sato, S., Kato, T., Tabata, S. & Wada, H.
(2002) Phosphatidylglycerol is essential for the development of
thylakoid membranes in Arabidopsis thaliana. Plant Cell ... by capillary
gas-liquid chromatography using a 50 m long, 0.25 mm
diameter CP-wax 52 column. Heptanoic acid was used as
an internal standard.
Results
The absence of functional PSII and lack of...
... Tsubouchi H, Naka D, Takahashi K,
Okigaki M, Arakaki N, Nakayama H, Hirono S,
Sakiyama O, Takahashi K et al. (1989) Molecular
cloning and sequence analysis of cDNA for human
hepatocyte growth factor. ... Shimomura T, Miyazawa K, Komiyama Y, Hiraoka
H, Naka D, Morimoto Y & Kitamura N (1995)
Activation of hepatocyte growth factor by two
homologous proteases, blood-coagulation factor XIIa
and hepatocyte ... S
& Daikuhara Y (1988) Purification and partial charac-
terization of hepatocyte growth factor from plasma of a
patient with fulminant hepatic failure. J Clin Invest 81,
414–419.
4 Miyazawa...
... Masayo Hosono
1,
*, Miyako Kitamura
1
, Tatsuya Tsuda
2
, Kiyofumi
Yamanishi
2
, Masatoshi Maki
1
and Kiyotaka Hitomi
1
1 Department of Applied Molecular Biosciences, Graduate School of Bioagricultural ... – 2A – 1A
QN
+ 1A + 2A + 3A + 4A + 5A + 6A + 7A + 8A + 9A
Relative value
0
0.2
0.4
0.6
0.8
1
1.2
1.4
Fig. 4. Assessment of the contribution of each amino acid residue
of K5 to substrate recognition. Alanine ... Bioagricultural Sciences, Nagoya University, Japan
2 Department of Dermatology, Hyogo College of Medicine, Nishinomiya, Japan
Transglutaminase (TGase; EC 2.3.2.13) is a Ca
2+
-
dependent enzyme that catalyzes...
... the characterization ofa member
of the SBP family from soybean [Glycine max (L) Merrill]
designated S64 or SBP2. Subcellular fractionation and pre-
cipitation by GTP-agarose demonstrated that S64/SBP2 ... Enhanced accumulation of SBP
caused an increase in intracellular sucrose synthase activity
with a concomitant decline in cell-wall invertase activity.
This alteration in sucrose-cleaving activities ... the identification of an isoform of soybean SBP,
designated S64 or SBP2, and we show that the SBP
homologue is a membrane-associated GTP binding protein.
We have generated mutants that blocked...
... Escherichia; Hafnia alvei;
diarrhea; neuraminic acid.
Hafnia alvei is a Gram negative bacterium and a member of
the family Enterobacteriaceae. There are reports of associ-
ation of H. alvei with diarrhoea ... and it was therefore concluded that the same
polysaccharide was present. A hydrolysate of the upper
phase, analyzed as alditol acetates, revealed as
D
-glucose,
D
-galactose,
D
-galactosamine,
L
-glycero-
D
-manno-heptose,
and
D
-glucosamine ... ofa prototypal
diarrheagenic strain of
Hafnia alvei
that has characteristics ofa new
species under the genus
Escherichia
Reine Eserstam
1
, Thushari P. Rajaguru
1,2
, Per-Erik Jansson
1
, Andrej...
... quinquestriatus hebraeus was collected
from scorpion stings to a parafilm membrane. Sarcophaga
falculata (blowfly) larvae and Periplaneta americana (cock-
roaches) were bred in the laboratory. Albino laboratory
ICR ... binding to rat brain
synaptosomes [23]. As AahIT4 shares little sequence
similarity with any of the known anti-mammalian scorpion
toxins [1,12], and no information was available on its mode
of action, ... various insect and mammalian sodium
channels [1,2,21,22]. As b-toxins that affect mammalian
sodium channels have not been identified in the ÔOld WorldÕ,
it was assumed that diversification of anti-mammalian
b-toxins...
... proportional to the rate of change, mutual inductance becomes
very significant in high-speed digital applications.
Mutual capacitance is the other mechanism that causes crosstalk. Mutual capacitance ... times larger than
the series resistance of the conductor.
3.1. MUTUAL INDUCTANCE AND MUTUAL CAPACITANCE
Mutual inductance is one of the two mechanisms that cause crosstalk. Mutual inductance ... vector network analyzer (VNA). In the frequency domain, the ac
resistance is often characterized by the attenuation factor, α, which is a measure of signal
amplitude loss as a function of frequency...
... cemetery of San Cataldo, Modena, Italy 135
C
ASE STUDY: Kresge College, University of California at
Santa Cruz, California, USA 138
C
ASE STUDY: Ghirardelli Square, San Francisco, USA 140
Urban objects ...
primary dimension of any categorization.
A further distinction can be made amongst urban design projects based on the
vocabulary of patterns that forms the basis of their design. The vocabulary, ...
in Ahmedabad. The individuals who have assisted me include El-Hassan Amr,
Oleksandra Babych, Clare Billingham, Kevin Brake, Giancarlo Cerutti di
Ludovico, Nick Chapin, Carol Chan, Tao Cheehai,...
... series of
questions. An average offline classification accuracy of
80% was achieved in 40% of the locked-in participants
using a non-linear discriminant classifier.
The ultimate goal ofa corporeal ... a baseline state can be detected. Using the
optimal feature set for each participant, mean classifica-
tion rates were calculated via 10 runs of 5-fold cross-vali-
dation, over a range of analysis ... optimal
classification accuracy was achieved for 8 of the 10 partic-
ipants with an LDA-trained classifier, which is advanta-
geous for its computational speed and ease of
implementation.
Quantifying...
... the
descriptive analysis, obesity had a high hazard ratio of
Table 2: Clinical and radiographic characteristics before and after UKA
Variables Pre-operative [SD] Final follow-up [SD] P value
a
Knee Society ... was per-
formed using antero-posterior, lateral, and Merchant view
radiographs of the knees (Figure 1B), with measurement
of femoral and tibial angles, alpha and beta angles, and
medial and lateral ... lateral joint spaces as described by Villers and
Cartier [7]. Patients were additionally evaluated for the
presence of patellar osteophytes as an indicator of patel-
lofemoral arthritis that is easily...