derivation and characterization of thyrocyte like cells from embryonic stem cells in vitro pdf
Báo cáo khoa học: "Effects of propranolol in combination with radiation on apoptosis and survival of gastric cancer cells in vitro" ppsx
Ngày tải lên :
09/08/2014, 09:20
... NY) containing 10% (v/v) heat-inactivated fetal bovine serum (Gibco, Grand Island, NY), penicillin (100 U/mL) and streptomycin (100 mg/mL), and they were maintained in a 37°C humidified incubator ... Analysis of the data and plotting of the figures were performed with the aid of software (Origin Version 7.5 and SPSS Version 13.0) Results Expression of b1- and b2-adrenergic receptors in SGC7901 and ... of Figure Apoptosis induction by isoproterenol or propranolol in combination with irradiation in BGC-823 and SGC-7901 cells There was an increasing rate of apoptosis in gastric cancer cell lines...
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Báo cáo khoa học: Identification and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf
Ngày tải lên :
07/03/2014, 12:20
... redox-active cysteine, the resolving cysteine (Cys-SR) In typical 2-Cys, Sp at the N terminus and SR at the C terminus of the protein belong to different subunits and condense to form an intersubunit ... found in A pernix because in the genome data base of P horikoshii neither a homologue of A pernix thioredoxin -like nor other types of thioredoxins were found In this study we examined the involvement ... terminus and SR at the C terminus belong to different subunits and condense to form an intersubunit disulfide bond; in atypical 2-Cys, Sp at the N terminus and SR at C terminus, originate from...
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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
Ngày tải lên :
07/03/2014, 16:20
... Biochem 271) model is in agreement with our CD data showing two minima at 205 and 215 nm, indicating that Rv1399c is composed of 22% of a-helices, 25% of b-strand and 39% of random coil The catalytic ... different ratio of E600 In each case, the kinetics of inactivation of Rv1399c have been monitored by measuring the remaining activity, as a function of time, using tripropionin as a substrate ... obtained from the pDest 17O/I construct using the BL21 (DE3)pLysS cells grew in LB medium after h of IPTG induction (Fig 1) Purification and refolding of Rv1399c Given the large quantity of insoluble...
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Báo cáo Y học: Identification and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf
Ngày tải lên :
08/03/2014, 16:20
... methionine residues were Met39, Met171, Met254, Met273 and Met352 It is known that the common sites of oxidation in proteins are histidine, lysine, proline, cysteine, arginine and methionine residues ... paradoxus strains By using the neighbour-joining, maximum-parsimony and maximumlikehood (Fig 1) methods from PHYLIP and testing the resulting trees using bootstrap analysis, the strain Iso1 specifically ... were from New BioLabs and Gibco BRL Pfu DNA polymerase and alkaline phosphatase were from Promega and Boehringer Mannheim, respectively D-Amino acid oxidase (EC 1.4.3.3) from porcine kidney and...
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Báo cáo khoa học: Genomic structure, expression and characterization of a STAT5 homologue from pufferfish (Tetraodon fluviatilis) ppt
Ngày tải lên :
31/03/2014, 07:20
... N-terminal protein interaction domain, coiled-coil domain, DNA-binding domain, SH2 domain, and C-terminal transactivation domain Furthermore, a tyrosine (Y698) was also identified in the C-terminal transactivation ... identical The boundaries of N-terminal protein interaction domains, coiled-coil domains, DNA-binding domains, linker regions and SH2 domains are indicated by arrowed brackets The tyrosine residue phosphorylated ... DNA-binding activity in vivo Fig Phylogenic tree of Tf STAT5 and other STAT family members The amino acid sequences of DNA-binding and SH2 domains of Tf STAT5 were aligned with those of nine known...
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Báo cáo hóa học: " Detection and characterization of chicken anemia virus from commercial broiler breeder chickens" pdf
Ngày tải lên :
20/06/2014, 01:20
... percentage of positive DNA from spleen, thymus and bone marrow make these organs an excellent choice of organs in the screening and diagnosis of flocks for CAV infection The finding of CAV DNA in embryos ... I and II (Fig 2) Seven of the isolates with amino acid profile of 75-I, 97-L, 139-Q and 144-Q were clustered together in cluster I while the remaining five isolates with amino acid profile of ... CIA-1 from USA, CAV-B from India, BD-3 from Bangladesh, isolate 704 from Australia, isolate 130 from Slovenia and NIE/19.04/118 isolate from Nigeria The remaining five current isolates including...
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Báo cáo hóa học: " Identification and characterization of alkaline serine protease from goat skin surface metagenome" docx
Ngày tải lên :
21/06/2014, 05:20
... and location of protease gene 4Fe-4S represents 4Fe-4S ferredoxin iron-sulfur binding domain protein, S8 & S53 - peptidase S8 and S53 subtilisin kexin sedolisin, sterol - Sterol-binding domain ... found in different strains of Shewanella, suggesting that the insert from metagenome could have been derived from a strain of Shewanella sp Majority of Shewanella sp are of marine origin (Fredrickson ... autoactivation of the recombinant cysteine proteinase EhCP112 from Entamoeba histolytica Protein Expr Purif 63:26–32 Page 10 of 10 Radha S, Gunasekaran P (2007) Cloning and expression of keratinase gene in...
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Báo cáo hóa học: " Preparation and characterization of spindle-like Fe3O4 mesoporous nanoparticles" pot
Ngày tải lên :
21/06/2014, 06:20
... preheated water in order to avoid nucleation of geothite during the initial heating stage [4,28] The synthesis of Fe3O4 NPs can be reached by reduction of a-Fe2O3 NPs in hydrogen atmosphere In brief, ... shape and size of the NPs are kept For further discussing the morphologies and structures of the samples, TEM images of S1, S2, S4, and S5 are presented, as shown in Figure It can be found in Figure ... ferromagnetism of magnetite In addition, the particle size and crystal morphology affect the coercivity in the order: spheres < cubes < octahedral in line with the increase in the number of magnetic...
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Báo cáo hóa học: " Preparation and Characterization of Nano structured Materials from Fly Ash: A Waste from Thermal Power Stations, by High Energy Ball Milling" potx
Ngày tải lên :
22/06/2014, 18:20
... blocks and tiles, in road making, in filling of the mines Attempts have been made earlier to utilize this fly ash waste in the polymer industry in making polymeric composites where fly ash is being ... analysis are shown in Table The percentage of alumina reduces marginally and the percentage of silica increases marginally as milling for 20 h, there after it remains unaffected with milling time TiO2 ... decreases and those of CaO and Fe2O3 marginally increased with long hours of milling Particle Size, Surface Area and Surface Energy Measurements of Fly Ash The variation in particle size and specific...
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Báo cáo khoa học: "Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus" doc
Ngày tải lên :
07/08/2014, 20:23
... rof g × 000,51 ta degufirtnec dna ,detacinos ,deretlif neht erew dna ,srotibihni esaetorp liatkcoc gniniatnoc ,2.7 Hp ,ATDE muidosid Mm dna )SBP( enilas dereffub-etahpsohp M 51.0 dloc ni dezinegomoh ... enomleilguG ,JA dlognaM ,MM ingoploV ,MM enurfaC ,OA nitalaS ,EA labañiV ,HD erriugA secnerefeR ASU ,sionillI fo ytisrevinU ,)AICUM( seitivitcA lanoitanretnI rof muitrosnoC seitisrevinU tsewdiM eht morf ... lacitirc rof ,tpygE ,oriaC ,ertneC hcraeseR lanoitaN ehT ,tnemtrapeD ygoloiB raluceloM ,demaH adeR aagaR rD knaht eW secneuqes dica onima lanimret-N gninimreted rof ,kramneD ,ytisrevinU kramneD...
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Báo cáo khoa học: "Expression and characterization of the UL31 protein from duck enteritis virus" pot
Ngày tải lên :
12/08/2014, 04:21
... Figure Intracellular location of DEV UL31 protein analyzed by indirect immunofluorescence Intracellular location of DEV UL31 protein analyzed by indirect immunofluorescence Mock and DEV-infected cells ... penicillin and 100 μg/ml streptomycin and were used throughout this study DEV CHv strain was a high-virulence field strain isolated from china, obtained from Key Laboratory of Animal Disease and ... location of the UL31 product in DEV-infected cells The intracellular distribution of UL31 protein was examined by indirect immunofluorescence staining At 36 h Page of 10 (page number not for citation...
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Báo cáo y học: " Systems-level comparison of host responses induced by pandemic and seasonal influenza A H1N1 viruses in primary human type I-like alveolar epithelial cells in vitro" pdf
Ngày tải lên :
12/08/2014, 11:22
... triple-reassortant swine influenza virus acquiring a neuraminidase and matrix (M) gene segment from viruses of the Eurasian-avian -like swine virus lineage [5,6] Since these swine viruses have in turn originated ... functions of individual candidates in influenza pathogenesis will require further investigation Lack of the control of transcriptional suppression by zinc finger proteins in pdmH1N1 infected cells ... nine of the genes down-regulated in response to seasonal H1N1 influenza virus (but not pdmH1N1) encode zinc finger proteins, including ZNF175 ZNF175 contains 13 zinc fingers and a KRAB domain,...
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Báo cáo sinh học: "Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus" pps
Ngày tải lên :
14/08/2014, 13:22
... areas of the genome may be over- or under-represented due to variation in HindIII sites in the selected range of insert sizes, or the inability to clone/maintain certain catfish genomic regions in ... genetic linkage map contains 243 type II (noncoding) and only 20 type I (coding) markers in 32 linkage groups, with an average inter-marker distance of 8.7 cM [28] However, a physical map of the ... g for 15 min, resuspended in 1.65 mL of Buffer L (100 mM EDTA, 20 mM NaCl, 10 mM Tris-HCl pH 8.0) containing 0.6% low melting agarose (InCert agarose, BMA, Rockland, ME), and poured into 75 µL...
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Báo cáo khoa học: Characterization of the glutamyl endopeptidase from Staphylococcus aureus expressed in Escherichia coli pptx
Ngày tải lên :
16/03/2014, 06:20
... cultured in LB broth containing 50 lgÆmL)1 of ampicillin and 25 lgÆmL)1 of kanamycin at 37 °C overnight Protein expression was induced by dilution of the culture with two volumes of LB broth containing ... active This may induce the cascade reaction of GluV8 activation, because recombinant proteins remain inside E coli cells, instead of being secreted from S aureus The conversion of amino acids adjacent ... mature form of GluV8 could be renatured even after exposure to heat in the presence of SDS Role of N-terminal Val69 in processing of the GluV8 proform Finally, the role of N-terminal Val69 of mature...
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Báo cáo khoa học: Structural characterization of L-glutamate oxidase from Streptomyces sp. X-119-6 pdf
Ngày tải lên :
23/03/2014, 05:22
... isoalloxazine ring of FAD is positioned at the interface between the FAD-binding domain and the substrate-binding domain Structure analysis revealed two funnel-shaped entrances (1 and in Fig 4A) ... (deaminating and decarboxylating) from Pseudomonas sp P-501 J Biochem 92, 1235–1240 Koyama H (1983) Further characterization of a novel l-phenylalanine oxidase (deaminating and decarboxylating) from ... 4A) extending from the surface of the protein into the interior, terminating at the active site near to the FAD cofactor The presence of the long funnel is a charcteristic of a number of flavoenzymes...
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Báo cáo Y học: Puri®cation and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli doc
Ngày tải lên :
24/03/2014, 00:21
... in lane 1, runs only marginally above the hA2aR fusion protein and is the main band in lane In this puri®cation, 7% of speci®c ligand binding sites loaded onto the XAC-agarose were detected in ... obtained, namely LysIle-Glu-Glu-Gly-Lys-Leu-Val-Ile-Trp corresponds to the N-terminus of the mature maltose-binding protein Binding of the fusion protein to the IMAC gel in buffer containing ... Radioligand binding-analyses on E coli and P pastoris membrane preparations, containing the S2 or the S4 somatostatin receptor fusion protein did not reveal speci®c binding of the agonist somatostatin...
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Báo cáo hóa học: "Synthesis and characterization of VO2-based thermochromic thin films for energy-efficient windows" pdf
Ngày tải lên :
21/06/2014, 04:20
... band Since intrinsic VO2 thin film is of n-type, introduction of ion donors cause an inevitable degradation of the transmittance (and resistivity) of the semiconducting low-temperature phase Likewise, ... in the VO2 solid solution The decrease in the IR modulation efficiency resulting from doping is mainly due to decrease in the transmittance in the semiconducting state This decrease is explained ... wavelength, in this case at l = 2500 nm The transition temperatures were then estimated by determining the first derivative of both curves of the hysteresis loops (heating and cooling) and considering...
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Báo cáo vật lý: "Synthesis and Characterization of Bismuth Tantalate Binary Materials for Potential Application in Multilayer Ceramic Capacitors (MLCC)" doc
Ngày tải lên :
07/08/2014, 14:20
... Pellets of single phase sample were prepared using a stainless steel die measuring mm in diameter Sufficient amount of powder was added, cold pressed uniaxially, and sintered at 1100oC in order to increase ... frequencies in spectroscopic plots of the imaginary components of the impedance (Z'') and electric modulus (M''), as shown in Figure The frequency maxima of Z'' and M'' should be coincident, and the ... frequency region with the increase of measuring temperature; this indicates the presence of polarization process in the dielectric material.15 The broadening of the peaks with increasing temperatures...
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Báo cáo khoa học: " No supra-additive effects of goserelin and radiotherapy on clonogenic survival of prostate carcinoma cells in vitro" ppsx
Ngày tải lên :
09/08/2014, 10:21
... induced by goserelin incubation and irradiation in LNCaP cells In PC-3 cells, we found more expression of this protein after incubation with goserelin than after irradiation alone Goserelin incubation ... combination of goserelin with radiotherapy in PC cannot be attributed to an increase in radiosensitivity of PC cells by goserelin in vitro It is to be explained by the synergistic effects of AD and ... to explain the clinically observed increase of overall survival in patients after combined therapy Recent studies investigated the effects of AD and radiotherapy in PC cell lines in vitro [summarized...
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Báo cáo khoa học: "Molecular characterization of two hantavirus strains from different rattus species in Singapore" ppsx
Ngày tải lên :
12/08/2014, 04:21
... LCN participated in the study design, fieldwork and coordination, and contributed to writing GB participated in the coordination of virus characterization, sequencing and drafting of the manuscript ... hosts of the Seoul virus Singapore strains and the viruses of the Jurong strain were identified as rodents of the R norvegicus and rodents of the R diardii clade, respectively An interesting point ... samples of kidneys and/ or lungs of individual rodents using Qiagen RNeasy Mini kit (Qiagen, Inc., Germany) or the TRIzol reagent (Invitrogen Inc., USA), according to manufacturer’s instructions...
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