define vapor pressure and boiling point of a liquid
Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx
Ngày tải lên :
18/02/2014, 08:20
... molecular basis of enzyme thermosta-
bility. J Bacteriol 185, 4038–4049.
20 Nanba H, Yajima K, Takano M, Yamada Y, Ikenaka
Y & Takahashi S (1997) Process for producing d-N-car-
bamoyl -a- amino acid. ... molecular mass
was also estimated by SDS–PAGE.
Characterization and comparative analyses of
HYD
Js
and HYD
Bp
The optimal temperature for activity of HYD
Js
with d-p-
HPH as substrate was determined ... Metab Dispos 2,
103–112.
11 Moller A, Syldatk C, Schulze M & Wagner F (1988)
Stereo- and substrate-specificity of a d-hydantoinase
and a d-N-carbamyl amino acid amidohydrolase of
Arthrobacter...
- 14
- 621
- 0
Tài liệu Báo cáo khoa học: Purification and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx
Ngày tải lên :
18/02/2014, 17:20
... semipreparative column was from Agilent Tech-
nologies (Santa Clara, CA, USA), and trifluoroacetic acid
and acetonitrile used for HPLC were from Merck (Darms-
tadt, Germany). Trypsin and tosyl phenylalanyl ... 2008)
doi:10.1111/j.1742-4658.2008.06352.x
Cone snails, a group of gastropod animals that inhabit tropical seas, are
capable of producing a mixture of peptide neurotoxins, namely conotoxins,
for defense and predation. Conotoxins are mainly ... of conotoxins
and their analogs [24–27].
Most NOESY crosspeaks were assigned and inte-
grated, with concomitant cycles of structure calcula-
tions for evaluation of distance and angle constraint
violations...
- 12
- 616
- 0
Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx
Ngày tải lên :
19/02/2014, 06:20
... 5Â-CTTTATTTTCAGGGCGCCATGAAGCGCG
CAAGACCGTCTGAA-3Â and a reverse oligomer 5Â-AGCT
CGAATTCG GATCCGGTACCTCAGAAGGTAGACAG
CAGAACC-3Â. For derivitization with TMR, a Gly-Gly-Cys
sequence was introduced at the C-terminus ... addition to an N-terminal Nco1 cloning site.
The forward o ligomer 5 Â-TCCGAAACCAGCG GCCGCTT
TATCGCGTTA AAAC CGGT GATCAA ACCCC -3Â and the
reverse oligomer 5Â-GTAGGCCTT TGAATTCCTCAAAA
AGTGCGGCTCGAT-3Â ... oligomers
5Â-CTGCTGTCTACCTTTGGAGGTTGCTGATCCGAA
TTCGAG-3Â (forward) and 5Â-GCTCGAATTCGGATCAG
CAACCTCCAAAGGTAGACAGCA-3Â (reverse). BL21
cells were transformed with the pETM11 construct and
grown until a D of 0.8...
- 10
- 647
- 0
Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf
Ngày tải lên :
20/02/2014, 01:20
... Salmon testes
DNA and some analytical grade chemicals such as EDTA,
Tris ⁄ HCl, CaCl
2
, NaCl and mineral oil were obtained from
Sigma (St Louis, MO, USA). Salmon testes DNA for the
enzyme activity ... folding pathway: structure-based analysis of
staphylococcal nuclease. Proteins: Structure, Function
and Genetics 27, 171–183.
15 Flanagan JM, Kataoka M, Fujisawa T & Engelman
DM (1993) Mutations ... Institute of Physics, Academia Sinica, Taipei, Taiwan, ROC
3 Department of Biochemistry, University of Minnesota College of Biological Sciences, St Paul, MN, USA
Staphylococcal nuclease (SNase) is a...
- 7
- 551
- 0
Tài liệu Báo cáo khoa học: Purification and cDNA cloning of a cellulase from abalone Haliotis discus hannai ppt
Ngày tải lên :
20/02/2014, 23:20
... ATCCTCACGAACAAGCAG
5RACR GATCGCGATGCAGGCCTT
FLF1 GGACGACTACAGCGTCTTCAGTAGA
FLR1 TCCAAACAGTCAGTTTCTTAACCGT
Table 1. Purification of cellulase from abalone Haliotis discus hannai. Oneunitofcellulasewasdefinedastheamountofenzymethatliberates
reducing ... The translational start codon ATG, termination codon TAA, and a putative polyadenylation signal AATAAA are boxed. A
putative signal peptide is indicated by a dotted underline. The amino-acid sequences ... RTARAAYTGNCCNGCRTCYTG
LP6 WAVEQMNYILGDNK R2 CATYTGYTCNACNGCCCAYTT
LP7 AWAWALGWDDK
LP8 GYHENA
LP9 WPLDYFL
F2 GCCACACTTCTGTCAACATCC
3RAC TTCTTCAAGGGCTGGCTCCCT
3AP CTGATCTAGAGGTACCGGATCC
5RACF ATCCTCACGAACAAGCAG
5RACR...
- 8
- 511
- 0
Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt
Ngày tải lên :
21/02/2014, 00:20
... Chicester.
32. Mu
ă
hlradt, P.F. & Frisch, M. (1994) Purification and partial bio-
chemical characterization of a Mycoplasma fermentans-derived
substance that activates macrophages to release nitric oxide,
tumor ... confers
interspecies variation of a major surface lipoprotein and a
macrophage-activating lipopeptide of Mycoplasma fermentans.
Infect. Immunol. 67, 760–771.
34. Takeuchi, O., Kaufmann, A. , Grote, K., Kawai, T., ... mechanical
disturbance leading to a conformational change of the
protein and, with that, signal transduction.
Recently, Ben-Menachem
3
et al. [45] presented a physico-
chemical characterization of...
- 9
- 665
- 1
Tài liệu Báo cáo Y học: Antibacterial and antifungal properties of a-helical, cationic peptides in the venom of scorpions from southern Africa pptx
Ngày tải lên :
21/02/2014, 15:20
... isolation and charac-
terization of amphipathic a- helical peptides from the venom
of Opistophtalmus carinatus, a scorpion living in southern
Africa, and we have made a comparative analysis of
the ... synthesized
chemically and preliminary studies on antibacterial activity
showed that the quality and biological activity of native and
synthesized toxins were identical. Due to a shortage of
native material, all ... [20],
parabutoporin and opistoporin 1 were tested on Gram-
negative and Gram-positive bacteria and their activity was
compared with the activity of melittin and mastoparan
(Table 1).
Parabutoporin...
- 12
- 598
- 0
NATIONAL REPORT OF MALAYSIA ON THE FORMULATION OF A TRANSBOUNDARY DIAGNOSTIC ANALYSIS AND PRELIMINARY FRAMEWORK OF A STRATEGIC ACTION PROGRAMME FOR THE BAY OF BENGAL potx
Ngày tải lên :
06/03/2014, 15:21
... of a federation of 11 states in Peninsular Malaysia and the states of
Sabah and Sarawak in the north of Kalimantan. Kuala Lumpur, the national capital, Labuan
UNEP/SCS – National Report Malaysia ... part of South-East Asia and occupies a total land
area of 330,434 square kilometres. The land mass comprises three main components:
Peninsular Malaysia and the two states of Sabah and Sarawak, ... waters. As
coastal aquaculture systems are located mainly in sheltered coastal waters of the Straits of
Malacca, these agricultural wastes, carrying bacteria and heavy metals, can be a health...
- 88
- 581
- 0
Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf
Ngày tải lên :
07/03/2014, 11:20
... L, Hamid Q & Elias JA (2004) Acidic
mammalian chitinase in asthmatic Th2 inflammation
and IL-13 pathway activation. Science 304, 1678–
1682.
4 Kasprzewska A (2003) Plant chitinases ) regulation ... increase of antifungal function.
Biosci Biotech Biochem 66, 1084–1092.
35 Kawase T, Yokokawa S, Saito A, Fujii T, Nikaidou N,
Miyashita K & Watanabe T (2006) Comparison of
enzymatic and antifungal ... equal amounts of (GlcNAc)
3
and (GlcNAc)
2
;
the 80 : 20 anomeric ratio of the products indicates
that cleavage after sugar 2 or sugar 3 occurs approxi-
mately equally often.
Structure
The overall...
- 12
- 399
- 0
Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx
Ngày tải lên :
07/03/2014, 15:20
... The remaining 495 amino acids are
considered to constitute the lac1 mature protein giving a
calculated molecular mass of 53 212 Da. Two putative
polyadenylation signals (TATAAA and CATAAA) were
identied ... cycles of 94 °Cfor20s,52°Cfor20s
and 70 °C for 2 min; then a final extension at 72 °C
for 10 min. The primers for lac1 P
LAC1F
(5Â-AGCTTT
CATTCCCAGTGATTG-3Â)andP
LAC1R
(5Â-AACGAG
CTCAAGTACAAATGACT-3Â) ... Stimulation of laccase production by
Fig. 6. Transcription analysis of lac1 by RT-PCR (A) and total laccase
activity (B) during various stages of V. volvacea fruitbody development.
mRNA was extracted...
- 11
- 703
- 0
Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
Ngày tải lên :
07/03/2014, 21:20
... 5Â-CTAGACTCGAGCCTAAT
TTATATTTGCTCCTTGTGC-3Â. b-Actin primers were
designed as follows: forward 5Â-CTACAATGAGCTGCG
TGT-3Â and reverse 5Â-AAGGAAGGCTGGAAGAGT-3 ¢.
Cell survival and apoptosis analysis
For viability ... contain a KH
domain. RNA analysis and blast analysis indicated
that homologs and orthologs of VBARP exist, indica-
ting the presence of VBARP in diverse phyla such as
plants, yeast, and eukaryotes. ... (predictprotein:
http://www.cubic.bioc.columbia.edu/predictprotein).
RNA extraction and quantitative real-time
RT-PCR
Total RNA was extracted from human tissues and human
primary cells using Qiagen RNA purification kit (Qiagen,
Valencia, CA) and used in real-time...
- 12
- 561
- 0
Báo cáo khoa học: Characterization and cDNA cloning of a clofibrate-inducible microsomal epoxide hydrolase in Drosophila melanogaster potx
Ngày tải lên :
07/03/2014, 21:20
... of the ORF and
contained a SalIsite(5Â-CTACGTCGACGATGGCGAA
CATCTGGCCACGAATC-3Â); the reverse primer corres-
ponded to the 3Â-end of the ORF and contained an XbaIsite
(5Â-AGGCTCTAGATTTATGAGAAATTGGCTTTCTG
GAC-3Â).
Expression ... post-treatment. The poly (A) -RNA was extracted from the
larvae, and 300 ng mRNA of each sample was loaded on a gel. Actin
mRNA served as an internal marker to equate mRNA quantities.
Fig. 9. Alignment ... DIG Labelling Mix (Roche). To
prepare a DmEH probe, primers (5Â-ATGGCGAAC
ATCTGGCCACGAATC-3Â and 5Â-TTATGAGAAATT
GGCTTTCTGGAC-3Â) were used, and to prepare Actin
5C probe as an internal marker,...
- 10
- 378
- 0
Báo cáo khoa học: Structural and biological effects of a b2- or b3-amino acid insertion in a peptide Application to molecular recognition of substance P by the neurokinin-1 receptor ppt
Ngày tải lên :
08/03/2014, 08:20
... switch point
between agonist and antagonist structures [21–23].
By molecular mechanics calculations the conformers of
Ac-HGly-NHMe, Ac-b
2
-HAla-NHMe and Ac-b
3
-HAla-
NHMe have been generated and ... acid-substituted SP analogues [HGly9]SP,
[b
2
-HAla9]SP and [b
3
-HAla9]SP may adopt conforma-
tions around residue 9 that are analogous to those
adopted by a- amino acids (Gly, Ala, Sar, Pro). To explain
the ... varying /, w
angles in intervals of 10° and setting h angle to ) 60 ± 10°
and 60 ± 10°.Each/, h,andw-value was fixed by applying
a harmonic potential and the structures were minimized
(adiabatic...
- 11
- 860
- 0
Tìm thêm:
- hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam
- xác định các mục tiêu của chương trình
- xác định các nguyên tắc biên soạn
- khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn
- khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản
- khảo sát chương trình đào tạo gắn với các giáo trình cụ thể
- xác định thời lượng học về mặt lí thuyết và thực tế
- tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam
- điều tra đối với đối tượng giảng viên và đối tượng quản lí
- điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1
- khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam
- khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu
- nội dung cụ thể cho từng kĩ năng ở từng cấp độ
- xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct
- phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ
- mở máy động cơ lồng sóc
- mở máy động cơ rôto dây quấn
- các đặc tính của động cơ điện không đồng bộ
- hệ số công suất cosp fi p2
- đặc tuyến hiệu suất h fi p2