các quy định liên quan
Báo cáo khoa học: "Expression and characterization of the flavoprotein domain of gp91phox" potx
Ngày tải lên :
07/08/2014, 14:22
... line Blood, 1996, 87(5), 2005-10 Bradford MM A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem, ... of assay buffer containing 200 mM NADPH and 200 mM NBT was added to each well NBT reduction was quantified by monitoring absorbance change at 595 nm using an extinction coefficient of 12.6 mM−1 ... 1319(2-3), 139-46 24 Koshkin V, Lotan O, Pick E The cytosolic component p47(phox) is not a sine quanon participant in the activation of NADPH oxidase but is required for optimal superoxide production...
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Báo cáo khoa học: "Activation domain in P67phox regulates the steady state reduction of FAD in gp91phox" pot
Ngày tải lên :
07/08/2014, 14:22
... Annu Rev Biochem 1980, 49, 695-726 Bradford MM A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 1976, ... change at 550 nm An extinction coefficient at 550 nm of 21 mM-1 cm−1 was used to calculate the quantity of cytochrome c reduced [13] Spectrophotometric and fluorometric assays: Heme content was...
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Báo cáo y học: "A case-control study of rheumatoid arthritis identifies an associated single nucleotide polymorphism in the NCF4 gene, supporting a role for the NADPH-oxidase complex in autoimmunity" doc
Ngày tải lên :
09/08/2014, 10:21
... haplotype maps Bioinformatics 2005, 21:263-265 35 Simple Interactive Statistical Analysis [http://www.quantita tiveskills.com/sisa/calculations/bonfer.htm] 36 Gabriel SB, Schaffner SF, Nguyen H, Moore ... 50:3085-3092 40 Stolt P, Bengtsson C, Nordmark B, Lindblad S, Lundberg I, Klareskog L, Alfredsson L: Quantification of the influence of cigarette smoking on rheumatoid arthritis: results from a population...
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Báo cáo y học: "DEK binding to class II MHC Y-box sequences is gene- and allele-specific" pptx
Ngày tải lên :
09/08/2014, 01:23
... exposed to a phosphor screen overnight, and the bands were quantified using a Molecular Dynamics Storm 840 Phosphorimager with ImageQuant Software The data were fit via nonlinear least-squares ... binds to this sequence nonspecifically and with low affinity Quantitative assessment of rDEK binding to related DQand DR- Y-box sequences Quantification of the apparent dissociation constant (Kd[app])...
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Báo cáo y học: " Allele-specific copy number analysis of tumor samples with aneuploidy and tumor heterogeneity" ppt
Ngày tải lên :
09/08/2014, 23:20
... must be taken into account when analyzing most tumor samples Göransson et al proposed the CNNLOH Quantifier to estimate the proportion of normal cells and copy-number neutral LOH from allele-frequency ... Ekman S, Bergqvist M, Thomas A, Lambe M, Rosenquist R, Holmberg L, Micke P, Botling J, Isaksson A: Quantification of normal cell fraction and copy number neutral LOH in clinical lung cancer samples ... copy number determination in cancer cells from single nucleotide polymorphism microarrays based on quantitative genotyping corrected for aneuploidy Genome Res 2009, 19:276-283 Greenman CD, Bignell...
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Báo cáo y học: "A single-tube allele specific-polymerase chain reaction to detect T315I resistant mutation in chronic myeloid leukemia patients" pdf
Ngày tải lên :
10/08/2014, 21:23
... Sensitivity of DHPLC and direct sequencing methods were evaluated by determination of dilutions of known quantities of T315I mutant products and WT products with indicated percentages of mutant products ... Koren-Michowitz M, Ashur-Fabian O, Nagler A, Rechavi G, Cohen Y: High-throughput, sensitive and quantitative assay for the detection of BCR-ABL kinase domain mutations Leukemia 2007, 21:1318-1321...
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Báo cáo y học: "Analysis of adenovirus trans-complementationmediated gene expression controlled by melanoma-specific TETP promoter in vitro" ppsx
Ngày tải lên :
12/08/2014, 04:20
... products [51], and most importantly, also the type and dynamic range of analysis system utilized for quantification Enhancement values of > 50, e.g., were obtained by others when using luciferase assays ... human adenovirus type vectors J Virol 1993, 67:5911-5921 15 Dion LD, Goldsmith KT, Garver RIJ: Quantitative and in vivo activity of adenoviral-producing cells made by cotransduction of a replicationdefective...
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Báo cáo y học: " Intragenic transcriptional cis-activation of the human immunodeficiency virus 1 does not result in allele-specific inhibition of the endogenous gene" pdf
Ngày tải lên :
13/08/2014, 05:21
... parental HOS 143b and HOS_A4 expressed HMBOX1 at similar levels However, this assay was neither quantitative nor specific for the HMBOX1 allele carrying the integrated vector A similar approach ... to detect allele-specific transcription in the HMBOX1 locus carrying the integrated provirus a quantitative RT-PCR was developed according to the protocol of Han and collaborators [29] As shown ... HIV_spliced) PCR amplification was conducted in the presence of the dye CyberGreen for relative quantification of PCR products Transfection of Tat induced HIV-1 transcripts several folds with...
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PHÂN BIỆT GENOTYPE 1 và 6 BẰNG kỹ THUẬT ALLELE SPECIFIC RT PCR dựa vào VÙNG GEN NS5B của VIRUS VIÊM GAN c
Ngày tải lên :
20/08/2015, 11:28
... genotype HCV 3.3 Đánh giá quy trình Với quy trình phân type xây dựng, áp dụng 48 mẫu huyết dơng tính HCV lâm sàng để đánh giá khả hoạt động thực tế quy trình Các mẫu huyết đợc xác định genotype phơng ... chu kỳ) Các sản phẩm PCR genotype HCV đợc điện di gel agarose phát với chất đánh dấu huỳnh quang Ethidium Bromide Đánh giá quy trình phân biệt genotype mẫu huyết lâm sàng: Chúng đánh giá quy trình ... tác quy trình allele-specific RT-PCR đợc trình bày hình 3A Kết quy trình mẫu huyết chứng genotype đợc trình bày hình 3B Y học thực hành (873) - số 6/2013 3A 3B Hình 3A Sơ đồ bớc tiến hành quy...
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Báo cáo khoa học: Dynamics of a-synuclein aggregation and inhibition of pore-like oligomer development by b-synuclein doc
Ngày tải lên :
23/03/2014, 09:21
... and hexamers over time without (left panel) and with (right panel) the addition of b-syn (B) Semiquantitative analysis of levels of a-syn multimers over time (C–F) Electron microscopy analysis ... Francisco, CA, USA) as previously described [31] and analyzed in the versadoc imaging system using the quantity one software (Bio-Rad, Hercules, CA, USA) To investigate the ultrastructural characteristics...
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Nghiên cứu tính đa dạng của allele hla - DQA1 bằng kỹ thuật polymerase chain reaction sequence specific primers (PCR - SSP) ở dân tộc kinh miền trung - Việt Nam docx
Ngày tải lên :
25/03/2014, 03:22
... khẳng định mối liên hệ mật thiết HLA số bệnh tật nh: đái tháo đờng không phụ thuộc Isulin, Viêm cứng cột sống, viêm đa khớp dạng thấp, viêm khớp thiếu niên Vì nghiên cứu HLA có ý nghĩa quan trọng ... Nghiên cứu tính đa dạng hệ kháng nguyên bạch cầu ngời có vị trí quan trọng tiêu chí di truyền học vô chắn, ứng dụng nhiều việc xác định cái, bố mẹ, khoa học hình để tìm tội phạm, nghiên cứu nhân ... phút Xem phân tích kết đèn cực tím máy vi tính để chụp ảnh bảo lu kết Xử lý số liệu thống kê: dùng cách tính tần suất % theo Hardy - Weinberg để thống kê xử ý kết đạt đợc Bảng 1: Vị trí, kích thớc...
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báo cáo hóa học:" Whole blood assessment of antigen specific cellular immune response by real time quantitative PCR: a versatile monitoring and discovery tool" potx
Ngày tải lên :
18/06/2014, 15:20
... antigen driven cytokine production, antigen specific cytotoxicity and lymphoproliferation assays or quantitative real-time polymerase chain reaction (qRT-PCR) for the detection of cytokine gene expression ... performance of multi centre comparative studies [4-6] Detection of cytokine (CK) gene expression by quantitative RT-PCR (qRT-PCR) has been successfully applied to the monitoring of immune responses ... samples to magnify antigen specific immune responses from low numbers of T cells To provide reliable quantitative assessments, we spiked cells from a HLAA0201 restricted CD8+ CTL clone recognizing...
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Báo cáo hóa học: "Prognostic Impact of MiR-155 in Non-Small Cell Lung Cancer Evaluated by in Situ Hybridization" pot
Ngày tải lên :
18/06/2014, 16:20
... an automated platform (Prior) Representative and viable tissue sections were scored manually semiquantitatively for cytoplasmic staining on computer screen The dominant staining intensity in tumor ... knowledge, only three studies have investigated the prognostic impact of miR-155 in NSCLC, all using quantitative RT-PCR as the principal method [13,16,18] Yanaihara et al [16], also using the median ... results Recently, Voortman et al studied the prognostic and predictive values of a panel of miRs by quantitative realtime PCR in formalin-fixed paraffin-embedded tumor specimens from 639 resected...
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Báo cáo hóa học: " Chromosomal 16p microdeletion in Rubinstein-Taybi syndrome detected by oligonucleotide-based array comparative genomic hybridization: a case report" doc
Ngày tải lên :
21/06/2014, 19:20
... Arveiler B: Analysis of CREBBP gene deletions in Rubinstein–Taybi syndrome patients using real-time quantitative PCR Hum Mutat 23:278-284 9 Petrij F, Dauwerse HG, Blough RI, Giles RH, van der Smagt...
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