cytoplasmic sensing of viral double stranded rna and activation of innate immunity by rig i like receptors
Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot
... investigated the ability of dsRNA to inhibit CHH function in shrimps in vivo So far, there is little information concerning the use of RNA interference (RNAi) in crustacean species Recently it has ... interference by double- stranded RNA in Caenorhabditis elegans Nature 391, 806–811 30 Dzitoyeva S, Dimitrijevic N & Manev H (2001) Intra-abdominal injection of double- stranded RNA into Functional analysis ... head virus replication in penaeid shrimp cells by dsRNA Biochem Biophys Res Commun 334, 102–107 26 Edomi P, Azzoni E, Mettulio R, Pandolfelli N, Ferrero EA & Giulianini PG (2002) Gonad-inhibiting...
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Báo cáo y học: " Rapid isolation of mycoviral double-stranded RNA from Botrytis cinerea and Saccharomyces cerevisiae" pot
... killer-independent dsRNA plasmids in Ustilago maydis by a simple and rapid method of extraction of dsRNA Plasmid 1989, 21:216-225 10 Franklin RM: Purification and properties of the replicative intermediate ... is loaded directly in the column, eliminating the incubation time with the resin, and only about 20 minutes of elution are needed to obtain high purity dsRNA It is worth noting that in the original ... Hypoviridae and Totiviridae families, respectively [17,18] The purified dsRNAs of S cerevisiae 1743 are shown in Figure 3A The 4.6 kbp L-dsRNA of the L-A virus and its satellite 1.8-kbp M-dsRNA...
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Tài liệu Báo cáo khoa học: The double-stranded RNA-binding motif, a versatile macromolecular docking platform doc
... (synthesized in large quantities in viral infection) can result in protein dimerisation, and subsequent autophosphorylation and activation [49] The PKR kinase activity is inhibited by virally ... both RNA- binding [37–39] and protein function [31,39] It is important to point out that the register of binding of RNaseIII to the RNA is different from the one of XlrbpA In RNaseIII the dsRBM binds ... Catalytic domain involved a RNase III domain A fi I deaminase domain DEXH helicase domain Ser ⁄ Thr kinase domain None None None None None Characteristic functions dsRNA processing in RNAi ⁄ miRNA...
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Báo cáo Y học: Double-stranded RNA-activated protein kinase interacts with apoptosis signal-regulating kinase 1 Implications for apoptosis signaling pathways docx
... Phosphorylation of protein synthesis initiation factor eIF-2 in interferon-treated human cells by a ribosome-associated protein kinase possessing site-specificity similar to hemin-regulated rabbit reticulocyte ... characterization of the human double- stranded RNA activated protein kinase induced by interferon Cell 62, 379–390 Hovanessian, A.G (1989) The double stranded RNA- activated protein kinase induced by interferon: ... require kinase activity of PKR [29,30] However, PKR-KR decreased the autophosphorylation activity of ASK1 in vitro, and inhibited both the activation of p38 MAPK and apoptosis induced by ASK1 in...
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Báo cáo Y học: Ribosome-associated factor Y adopts a fold resembling a double-stranded RNA binding domain scaffold potx
... structural similarity to the pY proteins can be grouped as follows: (a) proteins with similarity to the double- stranded RNA- binding domain and (b) proteins with similarity to the C-terminal domain of ... Spo0B and RapB [32] A recent study has directly correlated increased motional flexibility with higher RNA- binding affinity in the double- stranded RNA- binding domain (dsRBD1) of human interferon-induced ... a potential RNA- binding site residing within the C-termini of two a helices A comparison with the recently published similar structure of the protein HI0257 from Haemophilus influenzae is also...
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Báo cáo khoa học: Double-stranded RNA-dependent protein kinase (PKR) is downregulated by phorbol ester ppt
... stomatitis virus, herpes simplex I and vaccinia virus, are also inhibited by H7, a broader PKC inhibitor [34], indicating that the activation of PKC in cells by virus is important for virus to infect ... [5] Viral infection results in the activation of PKC in cells [30–32] Entry of influenza viruses into cells is inhibited by a highly specific PKC inhibitor [33] Other viruses including vesicular ... & Koromilas AE (2001) Activation of the IkappaB alpha kinase (IKK) complex by double- stranded RNA- binding defective and catalytic inactive mutants of the interferon-inducible protein kinase PKR...
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Báo cáo y học: "Natural antisense transcripts with coding capacity in Arabidopsis may have a regulatory role that is not linked to double-stranded RNA degradation" ppt
... rice, and an enrichment for genes with alternatively spliced transcripts, indicative of a role for some COP antisense transcripts in splicing modification In addition, we detect a potential link ... polyadenylation site, and alternative splicing TSS variation was detected by comparing the starting genomic positions of the first exons of genes with more than one transcript, and variation in the ... selection of individual candidate loci for a detailed molecular analysis of the different dsRNA pathways Materials and methods Analysis of overlapping transcripts All Arabidopsis genome information,...
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Báo cáo khoa học: Apolipoproteins A-I and A-II are potentially important effectors of innate immunity in the teleost fish Cyprinus carpio pot
... mammalian HDL and its principal apolipoproteins A -I and A-II in antimicrobial or antiviral activities in vitro [13,14,30], these proteins have not been yet recognized as important effectors in innate ... contribution of HDL and its apolipoproteins in teleost fish innate immunity, an additional mechanism might involve the release of one or more antimicrobial peptides by limited proteolysis of HDL-associated ... apolipoproteins A -I and A-II in the in vitro antimicrobial activity of carp HDL Both proteins inhibit the Ó FEBS 2004 growth of Gram-positive and -negative bacteria, including fish pathogens, at micromolar...
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Updates in the Diagnosis and Treatment of Vasculitis Edited by Lazaros I. Sakkas and Christina Katsiari pptx
... PR3 and MPO in addition to chromatin and LL37 (an antimicrobial peptide with capabilities of activating dendritic cells) [112] In-vivo presence of NETs was shown in tissues (kidney biopsies from ... (1988) Anti-neutrophil cytoplasmic autoantibodies with specificity for myeloperoxidase in patients with systemic vasculitis and idiopathic necrotizing and crescentic glomerulonephritis N Engl ... mimic idiopathic vasculitis and the differential diagnosis is of paramount importance for the practicing physician Vasculitides are not uncommon diseases In fact, some vasculitides, such as giant...
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báo cáo hóa học: " Apolipoprotein E isoform-dependent dendritic recovery of hippocampal neurons following activation of innate immunity" pot
... Curtiss LK, Williams DL: A minimally lipidated form of cell-derived apolipoprotein E exhibits isoform-specific stimulation of neurite outgrowth in the absence of exogenous lipids or lipoproteins ... neuroprotection [29] While apoE isoform-specific interactions with Aβ peptides likely contribute to the stratification of AD by APOE, the mechanisms by which apoE isoforms may influence the clinical ... following innate immune activation through their differing actions in microglia and astrocytes This study sought to test that hypothesis in vivo by determining whether there are apoE isoform-specific...
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Báo cáo y học: "Do synovial biopsies help to support evidence for involvement of innate immunity in the immunopathology of Behçet’s disease" pot
... authors of this editorial include a PhD resident (JK) and internist-immunologists (JVL, PVD, and PVH), representing the staff of the clinical immunological section of the internal medicine department ... relative to SF from patients with PsA This Page of (page number not for citation purposes) emphasizes that innate immunity is also involved in early BD and interacts with adaptive immunity, as ... Baeten D: Distinct synovial immunopathologic characteristics of juvenile-onset spondylarthritis and other forms of juvenile idiopathic arthritis Arthritis Rheum 2006, 54:2594-2604 Page of (page...
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CROSSTALK BETWEEN CRP AND FICOLINS REGULATES INNATE IMMUNITY 1
... adaptive immunity Over all, the innate immunity and the adaptive immunity integrated into an intact sophisticated host immune system where innate immunity serves as a rapid and efficient frontline ... L- and M-ficolin-mediated signal transduction via their interaction with CRP 193 178 181 xiii LIST OF ABBREVIATIONS ABTS 2,2'-azino-bis[3-ethylbenzthiazoline-6-sulfonic acid AD Activation Domain ... local infection-inflammation condition triggers a strong CRP:L-ficolin interaction, eliciting autonomous complementamplification pathways which co-exist with and reinforce the classical and lectin...
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CROSSTALK BETWEEN CRP AND FICOLINS REGULATES INNATE IMMUNITY 2
... agree with a homeostatic role of M-ficolin in partnership with CRP The critical function of histidine residues in M-ficolin has been identified to regulate the pH-sensitive binding of M-ficolin to ... elucidation of the binding interface 187 and the infection-inflammation sensitive regulatory region of the M-ficolin provide insights into the bioactive centre of the M-ficolin molecule, which might ... recognition of M-ficolin by CRP and the inhibitory effect of CRP on IL-8 production under infection-inflammation condition will further prevent the movement of monocytes in vinicity (solid line) and...
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Báo cáo sinh học: " Possible active origin of replication in the double stranded extended form of the left terminus of LuIII and its implication on the replication model of the parvovirus" pptx
... of LuIII gesting that the left hairpin of LuIII does constitute a replication origin in the extended double stranded hairpin configuration Given the functionality of the left hairpin of LuIII as ... origin of replication in the extended double stranded form a replication model of LuIII can be predicted resulting in equivalent amounts of plus and minus DNA viral strands (figure 6) In this ... termini can explain the unique encapsidation pattern observed for LuIII hinting on the mechanism used by LuIII for the replication of its viral genome Methods Construction of LuIII Lt-Lt The LuIII...
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báo cáo hóa học:" Possible active origin of replication in the double stranded extended form of the left terminus of LuIII and its implication on the replication model of the parvovirus" ppt
... of LuIII gesting that the left hairpin of LuIII does constitute a replication origin in the extended double stranded hairpin configuration Given the functionality of the left hairpin of LuIII as ... origin of replication in the extended double stranded form a replication model of LuIII can be predicted resulting in equivalent amounts of plus and minus DNA viral strands (figure 6) In this ... termini can explain the unique encapsidation pattern observed for LuIII hinting on the mechanism used by LuIII for the replication of its viral genome Methods Construction of LuIII Lt-Lt The LuIII...
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Báo cáo y học: "Expression and function of inducible co-stimulator in patients with systemic lupus erythematosus: possible involvement in excessive interferon-γ and anti-double-stranded DNA antibody production" pot
... production of IFN-γ, IL-4 and IL-10 were determined by ELISA (a) Production of IFN-γ without stimulation (b) Production of IFN-γ with ICOS co-stimulation (c) The production of IL-4 and IL-10 with ... B7RP-1-Fc, indicating the involvement of ICOS-B7RP-1 interaction in anti-CD3 mAb plus JTA009 stimulation (Figure 3b) ICOS co-stimulation significantly increased the [3H]thymidine incorporation of peripheral ... switching in response to various antigens [13,47] The ICOS-B7RP-1 interaction in mice is involved in the initial clonal expansion of primary and primed Th1 and Th2 cells in response to immunization...
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Báo cáo y học: "APOBEC3G induces a hypermutation gradient: purifying selection at multiple steps during HIV-1 replication results in levels of G-to-A mutations that are high in DNA, intermediate in cellular viral RNA, and low in virion RNA" pps
... hypermutation was highest in viral DNA, reduced in cellular viral RNA (cRNA), and lowest in virion RNA (vRNA), indicating a gradient of hypermutation We surmise that purifying selection at multiple ... selection, a gradient of hypermutation exists, with the viral DNAs containing the highest levels of mutations, cellular viral RNAs containing intermediate levels of mutations, and viral RNAs containing ... D: Identification of an APOBEC3G binding site in human immunodeficiency virus type Vif and inhibitors of VifAPOBEC3G binding J Virol 2007, 81:13235-13241 Mulder LC, Harari A, Simon V: Cytidine...
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Integrating DNA sequence features for more accurate prediction of replication origins in some double stranded DNA viral genomes
... prediction of replication origins in some double- stranded DNA viral genomes A computational method to predict the likely locations of replication origins was developed in this thesis Empirical ... predicting the replication origins vary in viruses and other organisms We will review the methods of predicting replication origins in viruses in the next section 2.2.2 Prediction of Replication ... prediction of likely locations of replication origins in herpesviruses Sections 1.1 and 1.2 provide an overview of the motivation and background of our study In Section 1.1, the basic biological...
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Tài liệu Báo cáo khoa học: Single phosphorylation of Tyr304 in the cytoplasmic tail of ephrin B2 confers high-affinity and bifunctional binding to both the SH2 domain of Grb4 and the PDZ domain of the PDZ-RGS3 protein ppt
... were amplified by PCR from six pairs of overlapping synthetic primers containing the two restriction sites of NcoI and BamHI for the SH2 domain and the two restriction sites of BamHI and EcoRI for ... Bioscience) Identification of the binding site(s) on ephrin B2 for the SH2 domain of Grb4 Six synthetic peptides including ephrinB2(301–333) and its five individual tyrosine-phosphorylated derivatives, ... Madison, WI, USA), removing the original fusion carrier in the pET32 vector In order to facilitate protein purification, a His-tag with six histidine residues was placed at the N-terminus of the...
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the effect of treaties on foreign direct investment: bilateral investment treaties, double taxation treaties, and investment flows doc
... California, Berkeley His primary research interests are the evolution and persistence of institutions and the ways in which institutions enable and constrain actors His work in international political ... Foreign Relations Law, and coreporter of the Institute’s Project on International Jurisdiction and Judgments He is an elected member of the Institut de Droit International and of the International ... location decisions of global firms, has appeared in academic journals, including the American Economic Review, Review of Economics and Statistics, Journal of International Economics, and Canadian...
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