cytokines chemokines and growth stimulating factors is associated with the depletion of tia proteins

Báo cáo y học: "Nucleosome deposition and DNA methylation at coding region boundarie" doc

Ngày tải lên : 09/08/2014, 20:20

... and then averaged in a sliding window of 100 bp or within a 150-bp segment downstream of the start codon or upstream of the stop codon The percentage of G and C nucleotides was obtained for the ... ylation, reflecting the fact that CpG islands are typically unmethylated Indeed, 97.2% of these genes contained a CpG island within their promoter (-1,000 bp to 500 bp from the TSS) and 92.8% had a ... regions, potentially allowing access of the initiation and termination complex (Figure 1b) The nucleosome-free region at the TSS was followed by the +1 nucleosome However, the association of the +1...

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Characterization of high efficiency pseudo bilayer organic solar cells and

Ngày tải lên : 30/09/2015, 10:11

... calculated that the energy received by the earth in 1.5 hrs is more than the total consumption of the world in the year 2001 This comparison gives you an idea of the enormity of the energy that the earth ... potential of sustaining the growing energy need of our world Among other forms of energy, electricity forms the crux of the energy crisis in the world today and solar cells help solve this problem ... excitation of this dye results in the injection of an electron into the conduction band of the metal oxide The original state of the dye is then restored by electron donation from the electrolyte,...

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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"

Ngày tải lên : 25/10/2012, 11:18

... into and proliferate in the target tissues, which is the basis of therapeutic effects of MSCs In adults, MSCs remain the potentials of multi-directional and multi-functional differentiation, and ... necrosis Ischemic femoral head necrosis refers to a disease which results from interruption of blood supply to the femoral head resulting in ischemia, necrosis and collapse of femoral head This dishttp://www.medsci.org ... evaluate the feasibility and safety of intravenous implantation of allogeneic MSCs in the treatment of femoral head necrosis Our study may provide theoretical basis for the clinical application of...

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Therapeutic ultrasound as a potential male contraceptive: power, frequency and temperature required to deplete rat testes of meiotic cells and epididymides of sperm determined using a commercially available system doc

Ngày tải lên : 05/03/2014, 17:20

... rotation offset mm from the center of the transducer face The movement of the transducer mimics its use as a therapeutic device and results in an averaging of the field output over time The distance ... distance of cm between the bottom of the ultrasound chamber and the proximal portion of the scrotum Determining the true effective radiating area (ERA) of our transducers Beam plots acquired with ... area of the beam with energy equal to at least 5% of the peak beam energy when the distance between the hydrophone and transducer was set to 0.5 cm The ME7413 transducer with a nominal area of...

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Báo cáo khoa học: Human skin cell stress response to GSM-900 mobile phone signals In vitro study on isolated primary cells and reconstructed epidermis docx

Ngày tải lên : 07/03/2014, 11:20

... skin models distance of 13.5 cm (minus the height of the culture dish) from the UV lamp The apparatus calculates the duration of exposure as a function of the desired dose Tests on isolated human ... emitted by the phone) and decreases rapidly with depth Phone use is associated with a slight temperature increase ( °C in the skin of the pinna) [15] However, this is mainly due to heating by the phone ... cutaneous cells Detection of apoptosis Apoptosis induction is often associated with cell stress This type of cell death occurs in several stages In the early phase, this phenomenon is characterized by...

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Báo cáo Y học: Tyrosine sulfation and N-glycosylation of human heparin cofactor II from plasma and recombinant Chinese hamster ovary cells and their effects on heparin binding pot

Ngày tải lên : 08/03/2014, 22:20

... were filled with lL of this solution The tip of the capillary was placed orthogonally in front of the entrance hole of a QTOF II mass spectrometer (Micromass, Manchester, England) equipped with a ... be established whether the amounts of sLex containing oligosaccharides synthesized by the liver are accomplished by a concomitant increase in the levels of the a2 fi sialyltransferase and a1 fi ... Microheterogeneity of plasma glycoproteins heparin cofactor II and antithrombin III and their carbohydrate analysis Thromb Res 51, 97–104 11 Varki, A (1993) Biological roles of oligosaccharides: all of the theories...

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Stem CELLS and the FUTURE OF REGENERATIVE MEDICINE pdf

Ngày tải lên : 15/03/2014, 09:20

... or the brain, in the adult body They can renew themselves in the body, making identical copies of themselves for the lifetime of the organism, or become specialized to yield the cell types of the ... the charter of the National Academy of Sciences, as a parallel organization of outstanding engineers It is autonomous in its administration and in the selection of its members, sharing with the ... potential to affect the lives of millions of people in the United States and around the world This research is now regularly front-page news because of the controversy surrounding the derivation of...

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Báo cáo khoa học: Complex gangliosides are apically sorted in polarized MDCK cells and internalized by clathrin-independent endocytosis doc

Ngày tải lên : 16/03/2014, 04:20

... rafts at the level of the TGN [19,20] The prevention of the association of apical proteins with lipid rafts perturbs the apical sorting of these proteins [21] Polarized distribution of sphingolipids ... by exposing the plate to iodine vapour The position of GM1 is indicated on the right of the plate Lipids migrated as multiple bands on the HPTLC plate because of the heterogeneity of the fatty ... clathrinmediated endocytosis Eps15 is an established component of clathrin-coated pits that is ubiquitously and constitutively associated with adaptor proteins; the expression of dominant-negative...

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Báo cáo Y học: Group IID heparin-binding secretory phospholipase A2 is expressed in human colon carcinoma cells and human mast cells and up-regulated in mouse inﬂammatory tissues doc

Ngày tải lên : 18/03/2014, 01:20

... more than 50% of the native enzyme (Fig 1C) The distribution of the KE2 mutant between the two fractions was similar to that of the native enzyme (Fig 1C) In contrast, the activity of the KE2RS2 ... reveals the presence of the TATA box and the binding motifs for AP-1 and NFjB in the putative 5¢-flanking promoter region of the human sPLA2-IID gene, consistent with its proinflammatory signal -associated ... the ears as well as significant elevation of serum IgE levels, accompanied by the increased TH1 response for the onset of skin dermatitis and the TH2 response in the lymph node [40] The ears of...

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Báo cáo khoa học: LRRK2 in Parkinson’s disease: function in cells and neurodegeneration doc

Ngày tải lên : 23/03/2014, 04:20

... activity), it is relatively clear that the kinase activity of b-RAF is the oncogenic activity associated with the protein [6] Other pathogenic LRRK2 mutations localize to the Ras of complex proteins ... factor receptor associated factor-2 and RIP1 in the formation of complex II The speciﬁc LRRK2 domain that interacts with FADD is not known, and the sensitivity of the interaction with intrinsic ... phosphorylation with LRRK2 over-expression or over-activity One issue with the published set of in vitro LRRK2 substrates that include moesin, MAPKK proteins and 4EBP1 is that the proposed phospho-residue...

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Báo cáo khoa học: Prohibitin is expressed in pancreatic b-cells and protects against oxidative and proapoptotic effects of ethanol pdf

Ngày tải lên : 29/03/2014, 08:20

... concentrations of ethanol The results are expressed as a percentage of the control (no ethanol) and shown as the mean ± SEM N = experiments *P < 0.05 versus control sis [28], we then determined the level of ... cellular distribution of recombinant His-tagged PHB Using a ﬂuorescence microscope, the His-tagged PHB did not localize to the nucleus, but showed a perinuclear distribution and colocalized with the ... in these cells Although PHB is known to be expressed in many tissues, this is the ﬁrst report of its expression in pancreatic b-cells Under normal conditions, PHB is found in cell nuclei and the...

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Báo cáo khoa học: Poneratoxin, a neurotoxin from ant venom Structure and expression in insect cells and construction of a bio-insecticide pot

Ngày tải lên : 30/03/2014, 13:20

... potential of 20 kV, a 94% grid potential, a 0.15% guide wire voltage, and a delay time of 100 ns Each spectrum is the results of 100 averaged laser pulses Aliquots of lL samples and of lL of a ... oligonucleotides the sequence of the poneratoxin gene is in bold letters and that of signal peptide is underlined The ﬁnal DNA products were digested with BamHI and EcoRI and inserted into the vector ... synthesized and puriﬁed by HPLC Mass spectrometry analysis of the product gave the correct molecular mass of 2756 (theoretical 2757) This peptide was used for structural studies and to raise the...

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Báo cáo khoa học: Differential regulation of fatty acid amide hydrolase promoter in human immune cells and neuronal cells by leptin and progesterone pdf

Ngày tải lên : 30/03/2014, 15:20

... immune system This differential regulation of FAAH suggests that the same factors can lead to a cell-specific control of the tone [24] and the activity of AEA along the neuroimmune axis, with important ... Fig Effect of leptin and progesterone on FAAH activity and expression (A) Effect of leptin on the activity of FAAH in human U937 and CHP100 cells and on the protein content and the mRNA of FAAH in ... immunoreactive band in U937 and CHP100 cell extracts, with an apparent molecular mass of 110 kDa (Fig 2B) This value is consistent with the expected molecular mass of the long form of LR [1,33]...

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Neural Stem Cells and Therapy Edited by Tao Sun potx

Ngày tải lên : 30/03/2014, 23:20

... the following headings 2.1 Tissue dissection methods The origin of the tissue influences the type of isolated cells as well as their proliferation and differentiation capacity A number of profound ... multipotentiality The main argument of these authors is the lack of fine dissection, and the inclusion of neural stem cells from other adjacent tissues, like SVZ Therefore, considering the current ... cases, the most important issue in terms of culture media is the use of either specific growth factors or serum The first works on NSPc isolation and maintenance described the use of serum in their...

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Báo cáo khoa học: Identiﬁcation of proNeuropeptide FFA peptides processed in neuronal and non-neuronal cells and in nervous tissue potx

Ngày tải lên : 31/03/2014, 07:20

... determined with synthetic NPFF (100 pmol per assay) The limit of detection of NPFF-IR material was estimated to be between and 12 fmol Analysis of the binding characteristics of the NPFF antiserum ... cell and tissue extracts, analytical characteristics of synthetic Ó FEBS 2003 peptides were initially determined on RP-HPLC and mass spectrometry Table shows the retention times of human and mouse ... of low intensity from the background noise at the retention time of synthetic SQA-NPFF (Figs 4A,B) The corresponding MS/MS spectrum (Fig 4C) displayed, in addition to the y4 fragment ion, b and...

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Tế bào gốc và ứng dụng trong y sinh học (Stem cells and the application in biomedicine) potx

Ngày tải lên : 02/04/2014, 22:20

... Identification of novel circulating human embryonic blood stem cells Blood 96, 1740 - 1747 IItskovitz - Eldor, J and et al (2000).; Differentiation of human embryonic stem cells into embryoid bodies comprising ... dừng trình tăng sinh Apoptosis trình chết theo chơng trình tế bào tế bào tự phá huỷ chúng không cần thiết chúng trở nên có hại Những tín hiệu đặc biệt gây nên apoptosis tế bào gốc cha đợc biết ... Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture Dev Biol 227, 271 - 278 Beltrami, A P., et al (2001); Evidence...

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cells and robots - milutinovic and lima

Ngày tải lên : 12/06/2014, 12:03

... decreases The amount of TCRs on the surface of the T-cell is a measure of the intensity of this interaction This amount changes with time and understanding of the dynamics of this change is an important ... of the population can be represented in the form of the distribution where on the x axis is the light intensity and y axis is the quantity of T-cells with a given light intensity x An example of ... me about it as well After the thesis and the antithesis, the synthesis ﬁnally came when, one day, discussing his work, we thought that exploring a mix of discrete event and continuous state space...

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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx

Ngày tải lên : 18/06/2014, 15:20

... important role in the recurrence and survival of HCC Therefore, the mechanisms by which Treg arise in vivo and exert their immunoregulatory effects remain to be defined and are the subject of intensive ... Treg and impair the induction of antigen-specific CTL in the presence of the supernatants Finally, we show that vaccination of the HCC patient with autologous FCs (DCs/auto-HCC) is associated with ... DCs/alloHCC/sp in the presence of the supernatants resulted in generation of Treg The abnormal function of DCs and differentiation into Treg in the local tumor microenvironment of the patient with advanced...

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báo cáo hóa học:" Generation in vivo of peptide-specific cytotoxic T cells and presence of regulatory T cells during vaccination with hTERT (class I and II) peptide-pulsed DCs" pot

Ngày tải lên : 18/06/2014, 15:20

... antigen uptake capacity and upregulation of CD86, CD40 and class I, but low levels of CD83, as is expected of non-terminally mature DCs The phenotypic profile of the DCTs and DCTIs generated from monocytes ... targets To the best of our knowledge, this is the first such finding from vaccination of cancer patients using DCs pulsed with these combinations of class I and II peptides of hTERT The study ... responses within individual patients, rather than patient groups Maintaining the levels of TAAg-specific CD8+ T cells in the circulation is the summation of the capacity to generate them and their...

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Báo cáo hóa học: "Lasers, stem cells, and COPD" docx

Ngày tải lên : 18/06/2014, 16:20

... regeneration of the rat mid-cortical diaphysis of the tibiae, which is a model of post-injury bone healing A small hole was surgically made with a dentistry burr in the tibia and the injured area and ... rats and the effects of daily He-Ne irradiation at 1.2 and 2.4 J/cm were assessed in comparison to 0.2% nitrofurazone cream The number of macrophages at day 16, and the depth of new epidermis at ... use light together with other agents to modulate endogenous stem cells The purpose of the current manuscript is to overview some of the previous work performed in this area that was of great interest...

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