culture of human pluripotent stem cells on glass slides for high resolution imaging
Atlas of Human Pluripotent Stem Cells doc
... long-term culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube ... of our team’s experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The exploration ... Morphology of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells Cultured in Feeder Layer-Free Conditions 3.1 Introduction 3.2 Materials for Feeder Layer-Free Culture of...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... stem cells versus embryonic stem cells The general differences between the characteristics of adult and embryonic stem cells are summarised in Table The contention that somatic stem cells alone ... statement in stem cell biology today is the importance of standardizing culture conditions Culture conditions have a profound effect on stem cell self-renewal, differentiation and possibly on stem cell ... isolation and characterization of hEG cell lines hEG cells are pluripotent and are capable of forming all primordial germ layers (Shamblott et al 1998; 2001) Embryonic stem cells Embryonic stem cells...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... induced pluripotent stem cell; H2AZ, histone variant H2AZ; H3ac, acetylation of histone H3; H4ac, acetylation of histone H4; H3K4me1/2/3, (mono-, di- and tri) methylation of lysine of histone H3; ... H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; H3K36me3, trimethylation of lysine 36 of histone H3; MacroH2A, histone variant MacroH2A; ... cell type of origin used to derive iPSC (c) H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3,...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... Marshall VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human ... with weak expression of SSEA3 and TRA-1-81 A total of 200 colonies were examined for each experimental group, as well as for the control Statistical comparison of data was performed by the Chi-squared ... differentiation in vitro Nat Biotechnol 2000;18(4):399-404 Richards M, Fong CY, Tan S, Chan WK, Bongso A An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... in human embryonic stem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine 2009, 7:20 human EB [17] depending on ... transcriptional network for pluripotency of embryonic stem cells Cell 2008, 132(6):1049-1061 Heo JS, Lee MY, Han HJ: Sonic hedgehog stimulates mouse embryonic stem cell proliferation by cooperation of ... undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational Medicine 2009, 7:20 18...
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Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf
... Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem cells (a) Temporal progression of bone morphogenetic protein (BMP)-2 and chordin expression during ... F, Avioli LV, Cheng SL: Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2 J ... 10 No Kwong et al Figure Transfection efficiency of siRNA in relation to concentration Final concentration of small interfering RNA (siRNA) in culture media in nanomoles/litre concentration (nM)...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... proximity of the cells to the joint space and the bone Page 10 of 15 (page number not for citation purposes) respectively, alignment of the chondrocytes along the arcades of Benninghoff [29] and ... level of BMP signaling is required for the onset of chondrogenesis, and hence some chondrogenic condensations failed to form in limbs deficient in both BMP-2 and BMP-4 [43] However, in the condensations ... although dispensable for bone formation, is required for the initiation of fracture healing Nat Genet 2006, 38:1424-1429 46 Goldring MB, Tsuchimochi K, Ijiri K: The control of chondrogenesis J Cell...
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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
... differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved stem cells ... from mesenchymal stem cells in Wharton’s Jelly of the human umbilical cord for transplantation to control type diabetes PLoS ONE 2008, 3(1):e1451 Liu M, Han ZC: Mesenchymal stem cells: biology ... increases the activation of Akt and ERK (A) MSCs were induced for differentiation without (Control) or with FN or LAM, and western blotting was done for stage III cells Quantification of western blotting...
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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
... USA) before culture to analyze cell proliferation T cells were resuspended in PBS 5% FCS at a concentration of to × 10 cells/ ml and incubated with CFSE (final concentration of 50 μM) for minutes ... treatment for CIA are inconclusive This is in contrast to transfer of Treg cells for the treatment of CIA When mice are injected with × 106 Treg cells either before immunization or after disease onset, ... http://arthritis-research.com/content/12/1/R31 Page of 11 Figure Phenotype and differentiation potential of mesenchymal stem cells (MSCs) Bone marrow cells of DBA/1 wild-type and interferon-gamma receptor...
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Engineered poly(l lactic acid) based nanofibers for osteogenic differentiation of human mesenchymal stem cells
... native bone (bone structure, bone cells, bone formation and bone repair), bone grafts and available bone graft substitutes, stem cell response to biomaterial topography, the importance of nanofibers ... images of bone nodules (indicated by arrows) produced by human MSCs of passage on the PLLA nanofibers and TCPS plates (control) in the Ost medium after 21 days of culture The cells on the nanofibrous ... nanofibers was considerably lower than on the TCPS plates In Figures c and d, the expression levels of BSP and WNT5A of the cells on the nanofibers were significantly higher than on the controls...
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Screen of nuclear receptors for the enhanced and alternative generation of induced pluripotent stem cells
... restriction of differentiated cells is undone The resultant cells, known colloquially as induced pluripotent stem cells (iPSCs), become akin to pluripotent embryonic stem cells (ESCs) and pluripotent ... SCREEN OF NUCLEAR RECEPTORS FOR THE ENHANCED AND ALTERNATIVE GENERATION OF INDUCED PLURIPOTENT STEM CELLS DOMINIC HENG JIAN CHIEN (B.Sc (Hons), NTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... in the modulation of gene regulation This transcriptional control is highly conserved from the simplest of model organism such as yeast to the highly complex of mammals such as us humans Strikingly,...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... proliferation and differentiation of rat mesenchymal stem cells Stem Cells Dev (4):661-670 69 Edlund U, Dånmark S, Albertsson AC 2008;A strategy for the covalent functionalization of resorbable polymers ... differentiation of human mesenchymal stem cells Exp Cell Res (1):22-37 101 Salasznyk RM, Klees RF, Boskey A, et al 2007;Activation of FAK is necessary for the osteogenic differentiation of human mesenchymal ... mesenchymal stem cells from marrow Tissue Eng (4):415-428 129 Beresford JN, Graves SE, Smoothy CA 1993;Formation of mineralized nodules by bone derived cells in vitro: a model of bone formation? Am J...
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Baculovirus mediated genetic modification of human embryonic stem cells
... the effects of the baculoviral transduction of the human embryonic stem cells were investigated The transduction of human embryonic stem cells by baculovirus vectors had no effects on the cell ... ABBREVIATIONS XIII CHAPTER ONE: INTRODUCTION 1.1 General Introduction 1.1.1 Human Embryonic Stem Cells 1.1.2 Non-Viral Genetic Modification Systems for hES Cells ... level of transient transgene expression in human embryonic stem cells Efficient gene transfers to human embryonic stem cells were obtained after optimization of the promoters and modulators Second,...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
... isolation and culture of ICM cells with stem cell-like morphology from human blastocysts (Fig 1.1A) though cultures failed beyond passages The first long-term culture (4-5 months) of human embryonic ... Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising hopes for effective cellular therapies to treat conditions ... identity of these cells was established by the expression of relevant markers like CD45, CD14, CD34, CD83 and CD86 and the formation of colony forming units in methylcellulose cultures The combination...
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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
... bone formation using Stem Cells 20 MATERIAL & METHODS 23 2.1 Culture and maintenance of Human Embryonic Stem Cells 23 2.2 Initiation of osteogenic differentiation ... Derivation of HES cells 15 1.5.2 Expansion and maintenance of pluripotency of HES cells 16 1.5.3 Characterization of hES cells 18 1.6 Differentiation of hES cells into ... of cells into scaffolds 12 1.3.4 Cell source for cell-based tissue engineering for bone regeneration 12 1.4 Stem cells in bone repair 13 1.5 Human Embryonic stem cells...
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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
... Summary V List of Tables VII List of Figures VIII List of Abbreviations IX Introduction 1.1 1.2 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 ... methods 22 2.1 Culture of human ES cell line 22 2.1.1 Preparation of feeder cells 22 2.1.2 Maintenance of human ES cells 22 2.1.3 Preparation of embryoid bodies 22 2.2 Culture of NCCIT cell line ... expression profiles upon differentiation and as such are not really ideal markers for assessment of the differentiation status of human ES cells (Bhattacharya et al., 2005) Analysis of expression profile...
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Human pluripotent stem cell derived cellular vehicles for cancer gene therapy
... Embryonic stem cells 20 1.2.1.1.2 Induced pluripotent stem cells 21 1.2.1.2 Adult stem cells 23 1.2.1.2.1 Mesenchymal stem cells 23 1.2.1.2.2 Neural stem cells 23 1.2.2 Clinical relevance of stem ... identified Pluripotent stem cells including embryonic stem cells and induced pluripotent stem cells may hold the answer to this question VIII From that point of view, we conducted investigations to ... Except for totipotent stem cells consisting of zygotes and their offspring cells from the first few divisions, pluripotent stem cells are the most powerful cells that are capable of giving rise to...
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báo cáo khoa học: "Atomic force microscopy: a powerful tool for high-resolution imaging of spermatozoa" ppt
... valuable information only if the alterations are gross and of the order of a micron or fraction thereof AFM provides the advantage of directly observing spermatozoa in their native environment thereby ... region may often lead to the loss of functional competence of the spermatozoa The major advantage of AFM in pathological studies of spermatozoa is that it allows the evaluation of position and form ... region c Power spectrum of the above profile showing the scaling of roughness d Histogram plot of the height of the head region e 3D image of the head region of the spermatozoa mechanical response...
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Establishment of autologous culture systems for human embryonic stem cells
... derivation sources, named embryonic stem cells Literature review (ESCs), embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem cells and induced pluripotent stem (iPS) cells ... cell source for future clinical applications Literature review Table Characterization of stem cells by derivation source Category Embryonic stem cells Embryonic germ cells Fetal stem cells Umbilical ... Figure Gene expression profiles of H9 ebF and H9 dF by conventional RT-PCR………………………………………………………………………88 Figure 10 ELISA analysis of H9-F conditioned medium for concentration of FGF-2………………………………………………………………………...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... transplanted cells derived from human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional culture on OP9 stromal cell line and examined the expression of VEGF-R2, ... transplantation of human mononuclear cells (hMNCs), which contain a very small population of EPCs (Ϲ 0.02%) [7], to examine the non-specific influences due to the cell transplantation itself The ... Neu-N (Chemicon, 1:200), and single stranded DNA (Dako Cytomation, 1:100) Immunohistochemical examination of cultured cells Staining of cultured cells on dishes at 5th passage was performed as described...
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