crystal structure of the low ph form of the vesicular stomatitis virus glycoprotein g187
Báo cáo sinh học: "Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" pot
... protein that phosphorylates ITAMs of the TCR zeta chain, allowing them to bind the zeta associated protein kinase ZAP-70 LCK then also activates ZAP-70 by phosphorylation, continuing the T cell ... 35–70% by pseudotyping the retrovirus with the envelope protein of another retrovirus, Gibbon ape leukemia virus In contrast with oncoretroviruses, the genus lentiviruses have the ability to transduce ... from the CD3 ζ (zeta) chain component of the T cell receptor Addition of the part of the small intracellular domain of the co-stimulatory receptor molecule CD28 has been shown to improve the responses...
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báo cáo hóa học:" Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" docx
... protein that phosphorylates ITAMs of the TCR zeta chain, allowing them to bind the zeta associated protein kinase ZAP-70 LCK then also activates ZAP-70 by phosphorylation, continuing the T cell ... 35–70% by pseudotyping the retrovirus with the envelope protein of another retrovirus, Gibbon ape leukemia virus In contrast with oncoretroviruses, the genus lentiviruses have the ability to transduce ... from the CD3 ζ (zeta) chain component of the T cell receptor Addition of the part of the small intracellular domain of the co-stimulatory receptor molecule CD28 has been shown to improve the responses...
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Báo cáo khoa học: Common mode of DNA binding to cold shock domains Crystal structure of hexathymidine bound to the domain-swapped form of a major cold shock protein from Bacillus caldolyticus pot
... shields the hydrophobic nucleobases and the hydrophobic-binding platform of the protein below them from the polar solvent (Fig 2B) The sugar of T1 from chain C maintains a C4¢-exo pucker, whereas the ... after evaluating both crystal structures we conclude that the alternative orientation of the base and sugar–phosphate backbone of the nucleotide in subsite and the formation of the alternative subsite ... allowing them to re-associate as domain-swapped dimers Apart from differences in the course of the protein backbone at the point of transition, in the swapped dimer the overall structure of the...
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Báo cáo khoa học: Crystal structure of the soluble form of the redox-regulated chloride ion channel protein CLIC4 doc
... Crystal structure of the soluble form of CLIC4 the backbone and side chain of Asn34 and the side chain of Lys24 This crystal contact stabilizes the structure of the leading side of the foot loop The ... that of the soluble form of CLIC1 (Fig 1B) [1], and thus belongs to the GST superfamily CLIC4(ext) is monomeric in the crystal and has approximate 4997 Crystal structure of the soluble form of ... the side chain of Asn81 The intervening residues of the C-terminal extension interact with the neighboring monomer via hydrophobic contacts Comparison with the structure of the soluble form of...
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Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt
... involving the Nf atom of TAgArg130 Because of the proximity of the Tyr205 hydroxyl group to importin-a, CLIC4 NLS peptide binding should be prevented by Tyr phosphorylation Therefore, the phosphorylation ... the side chain fits into the hydrophobic alcove and allows the charged head group access to the solvent on the other side The extended side chain of Arg206 allows for the same favourable interactions ... its location with the hydroxyl group reaching the importin-a main chain near the C-terminus of the ARM1 H3 helix Most of the hydrophobic contacts with importin-a residues lining the P4 pocket are...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... because the tertiary structure of ApeSOD has not been elucidated In the present study, for the first time, we describe the crystal structure of ApeSOD In particular, we focus on the coordination of the ... contents of the reconstituted enzymes were higher (Table 1), the crystallographic studies were performed on apo- and metal-reconstituted ApeSODs Crystallization and determination of structure The crystals ... NE2 of His31, bound to the metal, in the company of a water oxygen, from the apical positions The manga˚ nese was only 0.06 A out of the equatorial plane (Table 3) The angles around the metal cofactor...
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Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc
... PhyK as well as in AppA Although they are responsible for three hydrogen Crystal structure of Klebsiella phytase PhyK bonds to the 3-phosphate of phytate in PhyK, they also orient the 4-phosphate ... bonds with the 3-phosphate, Thr292 fixes the 2-phosphate The conserved HD dipeptide forms the N-terminus of a helix L The orientation of this helix allows substrate binding by hydrogen bond formation, ... molecules of the asymmetric unit of the wild-type structure Neither the mutation nor the different crystallization conditions evoked structural differences The crystal was grown in the presence of phytate,...
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Tài liệu Báo cáo khoa học: The crystal structure of human a-amino-b-carboxymuconatee-semialdehyde decarboxylase in complex with 1,3-dihydroxyacetonephosphate suggests a regulatory link between NAD synthesis and glycolysis ppt
... performed on the hACMSD crystals using the same beamline, clearly indicated the presence of a Zn metal ion bound to the enzyme Analysis of the diffraction data set allowed us to assign the crystal ... in the crystal, and a mixture of monomeric and dimeric forms in solution [18] Therefore, the available structural data suggest Fig Ribbon representation of the overall structure of hACMSD The ... ligand Trp191 shows the most pronounced movement, which consists of a rotation around the v2 dihedral angle of about 95°, allowing formation of a hydrogen bond with the DHAP phosphate group This...
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Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx
... to the residue in the midpoint of the respective loop, as shown in Fig To the east of the active site the 37- and 60-loops border the S2¢ pocket of the proteinase The observed differences in the ... (see below) The southern boundary of the active site cleft of DESC1 is formed by the 145 autolysis loop The backbone of this loop differs markedly from the other serine proteinases, making the active ... that the most similar regions of these proteinases mediate interaction of the two b-barrels, formation of the catalytic machinery and structures required for binding of the main chain of the substrate...
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Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc
... differences between the crystallographic temperature factors of the Fig Assembly of the BcZBP hexamer (A, B) Side and (C) top views of the BcZBP hexamer formed through the association of three dimers ... the presence of acetate in the crystal structure The binding of acetate in the active site is a further indication that the enzyme may be involved in deacetylation because acetate is one of the ... and determines the shape of the active site entry (e) The structure of the active site is essentially identical with the active sites of the MshB and LpxC proteins The conservation of catalytically...
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Tài liệu Báo cáo khoa học: ˚ The 1.8 A crystal structure of a proteinase K-like enzyme from a psychrotroph Serratia species docx
... similar arrangement, and the side chain of Ser97 in VPRK is too short One side of the loop is involved in the binding of the P2–P4 residues of a substrate The other side of the loop is close to Arg94 ... prime side of the binding site into a different conformation Conclusion The structure of a proteinase K-like enzyme of a psy˚ chrotroph Serratia species has been solved to 1.8 A The fold of the protein ... PRK One of the disulfide bridges in SPRK and VPRK is close to the S2 binding site It anchors a tight loop (see below) which is part of the S2 site, to the rest of the molecule, and may therefore...
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Tài liệu Báo cáo khoa học: Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid doc
... protein crystallography The fold of the subunit is similar to that of ScPDC [14] and ZmPDC [15] However, the packing of the two dimers in the tetramer is different from that of the PDCs of known structure, ... in all of the tetrameric PDCs of known three-dimensional structure Binding of the cofactors ThDP and Mg2+ The homo-tetrameric IPDC binds four molecules of the cofactors ThDP and Mg2+ The ThDP ... at the interfaces formed by the PYR domains from one subunit and the PP domains of the other subunit within the dimer ThDP adopts the V-conformation [30,31] and is completely buried in the cofactor...
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Tài liệu Báo cáo khoa học: The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol pptx
... resolution by the molecular replacement method The schematic view of the overall structure is shown in Fig 3A The enzyme exists as a dimer of the abc heterotrimer There is a noncrystallographic twofold ... around the center of Fig 3A The structure of an abc heterotrimer unit is shown in Fig 3B The central region of the a subunit constitutes the (b/a)8 barrel, the so-called TIM (triosephosphate isomerase) ... buffer (pH 8), components A and B were eluted successively with mL of 10 mM potassium phosphate buffer (pH 8) containing 40 mM KCl and then with 50 mL of 10 mM potassium phosphate buffer (pH 8)...
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Báo cáo khoa học: Crystal structure of salt-tolerant glutaminase from Micrococcus luteus K-3 in the presence and absence of its product L-glutamate and its activator Tris pdf
... initial phasing model for the N structure The G and TG structures were solved using the N structure as a search model, and the TG structure was used in phasing of the T structure The structures ... free R values of the four structures were in the range of the values of the published structures, those of the N and G structures were slightly poor [38,39] For the N structure, the extensive disordered ... a physiological dimer [10] was generated by a twofold axis of crystallographic symmetry The two independent physiological dimers shared a similar structure The physiological dimer of the TG structure...
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Báo cáo khoa học: The crystal structure of NlpI A prokaryotic tetratricopeptide repeat protein with a globular fold potx
... al Crystal structure of NlpI A B C D Fig Solubility of NlpI constructs and the structure of mature NlpI (A) 10–20% gradient SDS ⁄ PAGE of NlpI expression products, showing the insolubility of the ... Axis of noncrystallographic rotational symmetry runs through the center ‘x’ (C) Monomer of NlpI, showing the rolled-up array of helices with the C-terminus folding within the curvature of the ... of noncrystallographic symmetry running through the dimer interface We conclude that the contents of the asymmetric unit represent the biologically active protein The two chains together form...
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Báo cáo Y học: Crystal structure of the catalytic domain of a human thioredoxin-like protein pdf
... instead of a dimer in the case of TRX Furthermore, the loss of intermolecular disulfide-bonds and the disbandment of the hydrophobic patch may also obstruct the dimer formation (Fig 6) The1 4 residues ... Despite low sequence identity, dissimilar crystal forms and dissimilar intermolecular contacts near the active site in the crystal, the conformation of the active site (-Cys-Gly-ProCys-) of the hTRXL-N ... molecules, while the hydrophobic core consisting of b sheets shows little difference with other TRX (Fig 3B) Active site The location of the active site in all of the known thioredoxin structures is...
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Báo cáo khoa học: Crystal structure of the parasite inhibitor chagasin in complex with papain allows identification of structural requirements for broad reactivity and specificity determinants for target proteases pptx
... as the result of two factors: (a) the overall similarity of the enzymatic part, and (b) the variability of the angle of approach of the inhibitor relative to the catalytic cleft of the enzyme The ... out of the catalytic cleft The angle of approach, calculated in the way described above, has the lowest value for the tetragonal form of the chagasin–cathepsin B complex and the highest for the ... despite the lack of overall sequence similarity The role of the proline residue appears to be to maintain the specific shape of the loop The aromatic residue, on the other hand, interacts with the...
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Báo cáo khoa học: Crystal structure of the tetrameric inositol 1-phosphate phosphatase (TM1415) from the hyperthermophile, Thermotoga maritima docx
... inositol-polyphosphate-1-phosphatase and 3¢-phosphoadenosine-5¢-phosphate phosphatase activities: a novel target of lithium therapy J Mol Biol 315, 677– 685 14 Fieve RR (1999) Lithium therapy at the millennium: ... identifies the upper and lower dimers in the tetrameric structure In crystal form 2, the lower dimer has the active site loops in the b-hairpin conformation, whereas in the upper dimer the loops ... nonarchaeal IMPases The superposition of the second subunit of the MJ0109 structure (1G0H) after superposition of the first subunit requires a rotation of 15° along the longest axis of the dimer in...
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Báo cáo khóa học: Crystal structure of the chi:psi subassembly of the Escherichia coli DNA polymerase clamp-loader complex doc
... the proposed binding region of c inside the C-terminal collar of the clamploader complex conservation on the surfaces of the d and d¢ subunits suggests that the docking of the v:w unit onto the ... 271) Ó FEBS 2004 Fig Structure of the v:w heterodimer (A) Ribbon diagram of the v:w heterodimer crystal structure The w subunit is colored cyan and sits on top of the v subunit The v subunit is colored ... the v:w heterodimer onto the clamp-loader The structure of the clamp-loader complex is such that there is a prominent gap in the C-shaped base of the structure, between the d and d¢ subunits It...
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Báo cáo khoa học: The crystal structure of annexin Gh1 from Gossypium hirsutum reveals an unusual S3 cluster Implications for cellulose synthase complex formation and oxidative stress response potx
... crystal structure of Anx(Gh1) from cotton emphasizes the high conservation of the unique annexin fold even among the members of the plant subfamily of annexin proteins The fold is comprised of ... residue in the present structure is somehow halfway between the loop-in and the loop-out position of the bell pepper annexin Fig The three-dimensional structure of Anx(Gh1) (A) The fold of Anx(Gh1) ... are either distorted or the access of a cation to the site is blocked by the presence of a side chain of a basic residue In case of the IIAB site (Fig 2), the acidic residue acting as the bidentate...
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