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crystal form of the seven crystal systems

Báo cáo khoa học: Crystal structures of the apo form of b-fructofuranosidase from Bifidobacterium longum and its complex with fructose pot

Báo cáo khoa học: Crystal structures of the apo form of b-fructofuranosidase from Bifidobacterium longum and its complex with fructose pot

Báo cáo khoa học

... attack on the anomeric carbon of fructose. The leaving group iscarboxylate of Asp54.Dimerization The asymmetric units of the crystals of both the apoand complexed form of the enzyme consist of dimerswith ... maps. The crystal structure of the complex of b-fructofura-nosidase with fructose was solved by molecularreplacement using the crystal structure of the nativeenzyme as the search model. The ... blades in the axisarea are of polar character. The water channel in the apo form of the enzyme runs along the axis of the b-propeller through the whole domain and is onlyblocked by Cys236 in the...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

Báo cáo khoa học

... counted the interactions, we excluded all residuesinvolved in the binding of the metal cofactor.ApeSODs in different forms were superimposed with the least square fit of Ca atoms of the residues ... ligand,NE2 of His31, bound to the metal, in the company of a water oxygen, from the apical positions. The manga-nese was only 0.06 A˚out of the equatorial plane(Table 3). The angles around the metal ... additional water oxy-gen, which, together with the OD2 of Asp165, the NE2 of His79 and the NE2 of His169, formed anequatorial plane (Fig. 3B). The metal ion and the addi-tional water oxygen were...
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Tài liệu Báo cáo khoa học: Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase fromThermus thermophilus pdf

Tài liệu Báo cáo khoa học: Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase fromThermus thermophilus pdf

Báo cáo khoa học

... regulatory mechanism of Thr and the struc-tural features responsible for the high thermostability of TtAKb.Results and DiscussionModel quality The crystal structure of the Thr-bound form of TtAKb(TtAKb-Thr) ... good quality, with 95.4% of the res-idues in the most favored regions and 4.6% in allowedregions of the Ramachandran plot. The crystal structure of the Thr-free form of TtAKb(TtAKb-free) was ... binding shifts the equilibrium to dimer formation. In the absence of Thr, an outwardshift of b-strands near the Thr-binding site (site 1) and a concomitant loss of the electron density of the loop...
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Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

Báo cáo khoa học

... Ser214–Gly219 (the entrance frame), Lys224–Tyr228 (the back of the pocket) and the disulfide bridgeCys191–Cys220 (the front of the pocket) (Fig. 4A). The backbones of these segments form a deep ... below). The southern boundary of the active site cleft of DESC1 is formed by the 145autolysis loop. The backbone of this loop differs mark-edly from the other serine proteinases, making the act-ive ... revealsthat the most similar regions of these proteinases medi-ate interaction of the two b-barrels, formation of the catalytic machinery and structures required for binding of the main chain of the...
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Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

Báo cáo khoa học

... active site formation. Sections (6 A˚thick) of the protein surface that illustrate the shape and size of the active site. The sphere represents the zinc ion. (A) The main body of the active ... acetatewhich is the product of a deacetylation reaction. (c) The protein forms stable homohexamers both in the crystal form and in solution. Thus, the functionalstate of the enzyme is probably the hexamer. ... determines the shape of the active site entry. (e) The structure of the active site isessentially identical with the active sites of the MshBand LpxC proteins. The conservation of catalyticallyimportant...
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Báo cáo Y học: Crystal structure of the catalytic domain of a human thioredoxin-like protein pdf

Báo cáo Y học: Crystal structure of the catalytic domain of a human thioredoxin-like protein pdf

Báo cáo khoa học

... the Cys73–Cys73 disulfide bond [23]. The substitution of thesehydrogen bond forming residues in hTRXL-N may accountfor the formation of a monomer, instead of a dimer in the case of TRX. Furthermore, ... dissimilar crystal forms anddissimilar intermolecular contacts near the active site in the crystal, the conformation of the active site (-Cys-Gly-Pro-Cys-) of the hTRXL-N determined in the present ... little directeffect on the activity of the enzyme. The function of thisunique C-terminal domain remains unknown.Overall structureCrystals of the catalytic domain of hTRXL (hTRXL-N)were obtained...
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Báo cáo khoa học: Crystal structure of the parasite inhibitor chagasin in complex with papain allows identification of structural requirements for broad reactivity and specificity determinants for target proteases pptx

Báo cáo khoa học: Crystal structure of the parasite inhibitor chagasin in complex with papain allows identification of structural requirements for broad reactivity and specificity determinants for target proteases pptx

Báo cáo khoa học

... part, and (b) the variability of the angle of approach of the inhibitor relative to the catalyticcleft of the enzyme. The latter factor may reflect notso much the geometry of the catalytic site ... PW),despite the lack of overall sequence similarity. The role of the proline residue appears to be to maintain the specific shape of the loop. The aromatic residue,on the other hand, interacts with the ... [20,21]. The other segment of the guanidinium group of R91forms a pair of hydrogen bonds with the oxygen atom of the side-chain amide group of N18e. It is interestingto note that the equivalent...
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Báo cáo khoa học: Crystal structure of the tetrameric inositol 1-phosphate phosphatase (TM1415) from the hyperthermophile, Thermotoga maritima docx

Báo cáo khoa học: Crystal structure of the tetrameric inositol 1-phosphate phosphatase (TM1415) from the hyperthermophile, Thermotoga maritima docx

Báo cáo khoa học

... subunits of both crystal forms (diagonalterms). Please note the symmetry of crystal form 1 and asymmetry of crystal form 2 represented by low and high values of the rmsd,respectively.Subunita Crystal ... In the center there is a schematic of the mutual relationship of the monomers,in selected members of the family, as indi-cated by the twist angle of the dimer.TM1415 constitutes one of the ... 1. Schematic of the transformation of TM1415 into the eukaryotic FBPase. The transformation requires  25° rotation of the dimers around the tetramer axis and  30°rotation of the monomers in...
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Báo cáo khóa học: Crystal structure of the chi:psi subassembly of the Escherichia coli DNA polymerase clamp-loader complex doc

Báo cáo khóa học: Crystal structure of the chi:psi subassembly of the Escherichia coli DNA polymerase clamp-loader complex doc

Báo cáo khoa học

... but,like the d and d¢ subunits of the clamp-loader, neither vnor w contain any of the functional elements required fornucleotide binding. The topology of the w subunitresembles that of the bacterial ... of w,fromthelistofsequencesgiveninTable 3, is shown. The alignment is colored according to the degree of sequence conservation. These 26 residues are disordered in the crystal structure of the ... protein via the distal surface of v. The base of the clamp-loader complex hasan open C-shaped structure, and the shape of the v:w com-plex is suggestive of a loose docking within the crevice formedby...
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Báo cáo khoa học: Crystal structures of the human SUMO-2 protein at 1.6 A and 1.2 A resolution ppt

Báo cáo khoa học: Crystal structures of the human SUMO-2 protein at 1.6 A and 1.2 A resolution ppt

Báo cáo khoa học

... molecule to form an isopeptide bond with the Gly93 of another. The crystal structures of diubiquitin and tetraubiquitinshowed some alternatives of the quaternary c onformations of ubiquitin ... chains, theirconformations were adjusted with reference t o the NMRstructure of SUMO-1 and the crystal structure of yeastACDBFig. 2. Photographs and electron density maps of the SUMO-2 crystals. ... residues 45–58 in the former model and 40 , 49 and 55–58 in the latter model of SUMO-2 and the equivalents of ubiquitin. Although the sequences have only 18% identity, the protein folds of SUMO-2 and...
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Tài liệu Supply the correct form of the verbs1 docx

Tài liệu Supply the correct form of the verbs1 docx

Tài liệu khác

... live in a snake.14. My brother managed to kill the snake just at the time when I were almost exhausted. Supply the correct form of the verbs1. Cats could fly if they (have) wings.2. If Peter ... (not,want) to live in a snake.14. My brother managed to kill the snake just at the time when I (be) almost exhausted. Ifhe (be) a little late, I (kill) by the snake.15. Had I know you were ill, ... snake.14. My brother managed to kill the snake just at the time when I (be) had been almost exhausted. If he (be) had been a little late, I (kill) would have been killed by the snake.15. Had...
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Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt

Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt

Báo cáo khoa học

... after the transition of the enzyme to amore active form, respectively, t is the time and s is the lag-time. The rate constant, k, for the activation of the enzyme isobtained as 1 ⁄ s.CrystallizationCrystals ... lip prevented binding of the C-terminalresidues over the active site, or the absence of the C-terminal residues caused the movement of the lip(Fig. 1B). In addition, the Cachain between aminoacid ... between the dCTP(dUTP)-bindingconformer and the dTTP-binding conformer of dCTPdeaminase. We were not able to identify structuralchanges in the main chain of the subunit, or in the interaction of...
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