... of the D278 mutant indicates the partialcontribution of this side chain to the signals of the protonated acidic residues for the oxidized form in the absence of the inhibitor. The shifts of the ... respectively, fromthe Qobinding site(see Fig. 1), as measured fromthe chromone head-group of the inhibitor [2]. Their involvement in a waterchain leading up to the heme bLedge, and their parti-cipation ... observed for the Y302F and the Y297Fmutant enzymes. These shifts are rather small as com-pared to previously published absorption coefficientsfor these modes [23,25,28,29]. In the spectra of the Y302F...
... proteins,including other GHs (clans E, F and J) [3] and hemo-pexin [20]. The CD spectrum of the latter protein [21]is very similar to the one observed here; therefore, the unusual spectrum ... Avicel were obtained from Sigma-Aldrich, and the cello-oligosaccharides were obtained from Merck (Darmstadt, Germany). The oligosaccharidesderived from b-glucan were a gift from A Planas (Universi-tat ... incubation with Cel74A, and the proposed cleavage pattern (C) with the theoretical m ⁄ z value (z in superscript) of the most important fragments (the position of the Gal residues is arbitrary)...
... ODYSSEUS TO THE MAST the product itself, rather than from simply being offered the product. These effects support the point estimates discussedabove.In order to calculate the Treatment on the Treated ... effects estimated in the pre-vious specifications, and suggests that the impact of the commit-ment product came fromthe product itself, and not from the door-to-door marketing.Further supporting ... period.Comparing the treatment group to the control group, the largest treatment effects—in both the six-month and one-yearperiods—are for the very bottom of the distribution, the lowestdecile, and for the...
... stems from t he P3+cluster, then the g ẳ 2.27 feature derives fromthe FeFe c ofactor. On the other hand, if the broad signal, d espite its unusualproperties, represents the P1+cluster, the ... absent. The profile of the entire signal, without interference of the rhombic s ignal, was obtained by subtracting the pH 6.4-spectrum fromthe pH 8.4-spectrum (see the inset of Fig. 3). The axial ... of these novel EPR signals toeither the cofactor or the P-cluster has proven elusive due to the fact that both of these metal clusters present in the Fe-only n itrogenase are d iamagnetic in the...
... saturat-ing) and the variation of initial rate with the concen-tration of the second substrate is then used to estimatekcatand the Kmfor the variable substrate. However, the assumption that ... bound at the active site. The Y102A mutant therefore accomodates a variant config-uration of biliverdin to the wild-type enzyme butretains the ability to catalyse the transfer of hydride from both ... andvarying the concentration of NADPH from 5–100 lmor NADH from 50–1000 lm. The ‘fixed’ concentra-tions of biliverdin were also varied from 0.5–10 lm toyield the plot for NADPH as the variable...
... changes in the HSQC spectrum of the cÂe complex bymutating the cE204 residue to Gln. Inset in (B) indicates the changes in the selected areas of the HSQC spectrum of the cÂe complex imposed by the ... with the catalytic sites for ATPsynthesis, and the membrane-embedded Fodomain(bacterial subunits ab2c10–15), with the sites for the translocation of the ions. In ATP synthesis mode, the Fomotor ... empty chip), allowing the eliminationof the latter by baseline correction (see below). As a resultof the location of the His tag on the very top of the c sub-unit, the immobilized cÂe complexes...
... in the protein stability of our mutants. There-fore, these conclusions strengthen our hypothesis that the catalytic efficiencies of the recombinant enzymesused in our study were related to the ... transfer from the two-electron substrate NADPH to Fd pro-ceeds in an ordered pathway, in which the observedKmvalue results fromthe sum of the Kmvalues for the successive interaction of the ... by DNA sequencing. The design of the mutants was intended to preserve the amino acid character and to modify only the relativevolume of their R groups. The expression of the FNR mutants as soluble...
... private rather than public terms, relating themto the play of the imagination rather than the exercise of reason.But these shifts cannot erase the important continuities thatexisted between the ... elites it was intent on displacing on the onehand and fromthe various popular and plebeian strata it aspired to ruleon the other.19 The first of these shifts, from a focus on a cultural product(literature) ... prefaced with a statement of the editor’s commitment to the Enlightenment30Literature has reconciled the whole thinking world respecting the greatprinciples of the system of the universe, and extirpated...
... Figure 5C shows that the spectra of the sample subjected to compression and decompression aresimilar to the non-treated sample, even in the case of the M88V mutant. The elution of the samples after ... as the major virus structural protein,forming the shell in which the RNA genome iscontained [11,12]. The tertiary structure and topology of the MS2 coatprotein is different from those of other ... interacts with residues on the adjacent b-strand (F) andwith amino acids in the alpha-helical regions as they passover the b-sheet. Thus, the M88V mutant substitutionaffects the dimer interface,...
... prisoners, the nature of their offensesor the lengths of their sentences in a timely manner. Since the demographics and criminal historyof these offenders are unknown, they cannot be factored into the ... inmost other jurisdictions, the growth of the corrections system has had a profound effect on the state’s fiscal resources. The cost of the Oklahoma corrections system has tripled over the last ... open access to their facilities over the course of thisproject. Without the assistance of the many line staff and managers who took time from thereassigned duties to explain their operations...
... children, but they should also consider adults, as their competence to make healthy choices or their capacity to resist the marketing of unhealthy food may not fully protect them from the damage ... things. The dynamics of the food system, particularly in the expanding markets of eastern Europe, needs further ex-ploration and the evolution of the food supply, particularly the price and availability ... increased over the past decade (Fig. 10).4. The European Region has the highest alcohol consumption in the world, particularly among men (69). Wine is generally preferred in southern Europe,...
... exactly the Egypt of ancient days is described in the Bible! The dress the people wore, the food they ate, the way they spoke to their kings, the description of theirfunerals, the very name of their ... through the walls of the Holy City. His ears rang with thecries of rage and despair whichbroke fromthe Jews within, as one by one their defences fell, and the end drew near!Then food failed in the ... BEHIND THE CLERK'S EAR]So the questioners settled the matter in their own minds; but God had the answer to their questions all readyfor them.He put into the hearts of some brave men the...
... properties of the enzyme [19]. To extend the information about the role of the noncatalyticSBD regions and their effect on the C-terminal CD,we further explored the amino acids of the N-terminalregion ... Whereas the production of ADPGlc via ADPGlcPPase is the first committed step in starch biosynthe-sis, SS catalyzes the elongation of a-1,4-glucans by the transfer of the glucosyl moiety fromthe ... confirmed the results obtained in the pull-down assays, showing that there is a physicalinteraction between the N- and C-terminal domains ofSSIII in vitro.Mapping of the CD-binding region in the N-terminal...
... Consequently, the top two carbon atoms of s7p synthesized in the half-reaction model can originate from either f6p or p5p,while in the traditional model they can only originate from p5p. In ... proceduresMetabolic network modelApart fromthe variations in the stoichiometric model of the PPP discussed in this work, the other parts of the stoi-chiometric model used for fitting the fluxes of S. cerevisiaewere ... the metabolites formed from these) can differ from the 13Clabeling of the single C2and C3fragment pools gener-ated by the half-reactions.Genetic organization of the nonoxidative branchof the PPPIn...
... and acquisitions easier because it replaced most of the existing laws concerning these activities. The law furthermore exempted taxes on the surplus capital by liquidation of companies in case ... Market: The Brewing Case’ The Journal of Industrial Economics, 13-2 (1965), p.129-153.; BROUWER, M., The European Beer industry; Concentration and competition’ in: DE JONG, H.W.(ed.), The Structure ... and Concentration in the Brewing Industry’, The Journal of Industrial Economics, 19-3 (1971), p. 206.; PELES, Y., ‘Economies of scale in advertising beer and cigarettes’, The Journal of Business,...