... SIA It is thought that this interaction with SIA is responsible for cell to cell adhesion as sialidase often interrupts binding In particular, an arginine residue within the first Ig domain is a ... the preferred tyrosine for interaction with SHP-1 SHP-2 binding, however, was only diminished by < 50% in the Y667F mutant, indicating that SHP-2 may be binding both to the tyrosine at position ... eosinophil cell lysates (data not shown) are capable of binding to the cytoplasmic domain of the siglec-10 protein The binding of SHP-1, however, was missing from the Y667F mutant indicating this...
... to excellent use in preparing students to discuss a complex issue It is a technique whose purpose is to initiate some sort of thinking process Brainstorming involves students in a rapid-fire, ... giving instructions), monitoring (listening to the pairs/ groups during the activity and guiding / giving support/ making notes) and winding down (bring the activity to a close and providing ... mainly on developing reading and writing skills The students of English major classes use a curriculum which lays great emphasis on four skills including listening, speaking, reading and writing...
... concentration of hypericins in H perforatum tissues and HpPKS2 is thus a candidate gene for the biosynthesis of hypericins [25] In this study, the role of H perforatum HpPKS2 is investigated in more detail ... of hypericins, indicating that HpPKS2 may have a role in the initial key reaction step in the biosynthesis of hypericins in H perforatum However, although the enzyme is capable of carrying out ... observed in the leaf margins are also present in the petal margins, whereas the nodules in the interior parts of the petals are elongated tubulars [10,12] In this study, nodules in both the margins...
... conformation of inhibitor main chain Thus, different inhibitors differ not only in the binding affinity, but also in the degree of freedom of the inhibitor inside the binding tunnel Some special inhibitors ... polypeptide chains: chain A, Pro1-Phe99 and chain B, Pro101-Phe199 The inhibitor is bound as an extended chain in a tunnel running under flaps across the dimer interface The flaps consist of the aminoacid ... orientation in both cases is concerned In answer to the final question, the X-ray structures discussed in this paper showed that some even small changes in inhibitor chemistry can have a large in uence...
... No flavin compounds were bound to the a1 subunit of PDH1, though it did contain one ADPbinding motif This suggests the ADP-binding motif in the a1 subunit mediates the observed ATP binding, and ... similar structures consisting of two domains, one that binds FMN and one that binds FAD and NADPH [8–10] The FMN domain is homologous to flavodoxins, while the FAD and NADPH domain is homologous to ... containing the PDH1 gene (insert length; 8.1 kbp) and pPDH2 containing the PDH2 gene (insert length; 7.4 kbp), to be obtained and used as templates for DNA 4051 FAD, FMN and ATP-containing amino-acid...
... current interest in database interfaces and in which considerable research is needed Large, shiny nuggets of theory are waiting to be discovered by enterprising computational linguists! I Speech input ... linguistic forms and speech acts than when interfacing to a simple db system areas has been going on for some time and shows no sign of running dry One criticism of the simple db interfaces is ... database assistant could carry on a natural conversation with the user Generating language An intelligent database interface assistant should be able to interject comments as appropriate, in addition...
... [31], and l-arginine:glycine amidinotransferase (EC 2.1.4.1) involved in creatine biosynthesis in vertebrates [26] In cylindrospermopsin biosynthesis, the amidinotransferase CyrA is thought to ... binding site distinct from the hydroxylamine-binding site This binding affects the rate of reaction by factor b, causing the noncompetitive component of the mixed inhibition In addition, binding ... ornithine as amidino group acceptors This is the case in l-arginine:glycine amidinotransferase which only have one substrate-binding site, giving rise to the classical ping-pong mechanism and...
... abolished This could result in loosening of the stability of the structure in this part of the monomer Although tyrosine-42 is distant from subunit interaction interfaces, increased breathing ... structure is indistinguishable between the three proteins at this protein concentration Furthermore, this temperature-induced structural change was irreversible, since a return to 35 °C after heating ... proteins was also reflected as decreases in b-oxidation activities of cultured fibroblasts with increasing temperatures, indicating that this effect is observable in intact cells Interestingly,...
... determined by the TMPRED program They are indicated in white letters on black background The amino terminus end of the recombinant truncated proteins (D80-protein and D161-protein) is indicated ... proteins were analyzed by SDS/PAGE and Coomassie staining, no recombinant protein could be visualized Therefore, although the recombinant protein was present in low amounts in E coli, it was in ... protein, [(a) D80-protein missing the ®rst 79 amino acids and similar in size to the prokaryotic MgPIXMT; and (b) D161-protein missing the ®rst 160 amino acids] led to high amounts of recombinant...
... protein kinase and woundinduced protein kinase, two MAPKs in group A, were activated by inoculation with the tobacco mosaic virus (TMV) [10] Their orthologs in other plant species, including MPK3 ... and MPK6 in Arabidopsis (group A MAPKs), SIMK and SAMK in alfalfa, and LeMPK1 ⁄ (Lycopersicon esculentum SA-induced protein kinase) and LeMPK3 (L esculentum woundinduced protein kinase) in tomato, ... shown in Fig 7E, the discs incubated in water without MV showed no abnormalities After incubation in different concentrations of MV for 72 h, symptoms of bleaching or chlorosis appeared in the...
... recognise this distinction by a corresponding terminological one Since some of the artists exploring this domain have started referring to their discipline as “soundart” (Klangkunst in German), this would ... inspiration, in a strict sense, as an explaining factor A biologist might have insight into the process of discovering some new relation, but would never admit being inspired; a painter, even working in ... that music is not merely another kind of amusement, and in On the Musically Beautiful (1986, originally published 1854) he invested considerable argumentation in distinguishing and distancing it...
... serine–threonine protein phosphatase calcineurin [5] The brain is an especially interesting organ in which to examine RCAN1 expression, because calcineurin is highly expressed in this organ, comprising ... calcineurin activity in AD, and other data showing that calcineurin inhibition results in tau phosphorylation on serine and threonine residues consistent with those that occur in AD [9–13] In ... contain a putative calcineurin-binding motif (PKIIQT) [3,5] Calcineurin is a calcium-dependent serine– threonine protein phosphatase, which has several known substrates, including the transcription...
... spinach [5] These results indicated that an inserted loop region of stromal APX is involved in its hydrogen peroxide-mediated inactivation Discussion In this study, by using various chimeric APXs ... have proved the hypothesis that a region in the C-terminal half of stromal APX 2708 is involved in its susceptibility to hydrogen peroxide and indicated that this region isin a loop unique to chloroplastic ... microbody-localized APXs of higher plants [8] This finding raised the hypothesis that a region within the C-terminal half of the chloroplastic APXs is involved in their susceptibility to hydrogen peroxide...
... results in higher perceptual fiuency, and training in arts gives meaning to complex structures in paintings, poems, or music, which results in an additional increase in processing ease Accordingly, ... symmetry involving reflection around a vertical axis (as in capital "A" or "V"), symmetry involving reflection around a horizontal axis (as in capital "E" or "D"), or symmetry involving reflection ... target, resulting in more positive evaluations This assumption is consistent with the feelings-as-information model, which holds that feelings serve as a source of information in their own right,...
... injury is caused by opening of the mPTP There is evidence that ADP inhibits opening of the mPTP by occupying binding sites located in the inner and outer mitochondrial membrane [39,40] The binding ... relevance in brain, because in this tissue glucose is metabolized providing the NADH-yielding substrate pyruvate Under these conditions, some succinate is formed by the citric acid cycle, oxidizing ... micromolar Ca2+ generate H2O2 during reoxygenation that is released into the extramitochondrial space This isin line with in vivo studies of ischemia ⁄ reperfusion showing increased ROS production by...