creactive protein structural biology and host defense function
Báo cáo khoa học: Multifunctional host defense peptides: functional and mechanistic insights from NMR structures of potent antimicrobial peptides docx
Ngày tải lên :
29/03/2014, 22:21
... 6473
MINIREVIEW
Multifunctional host defense peptides: functional and
mechanistic insights from NMR structures of potent
antimicrobial peptides
Surajit Bhattacharjya
1
and Ayyalusamy Ramamoorthy
2
1 Biomolecular NMR and ... of
polymyxins B and E and effect of binding to lipopoly-
saccharide: an NMR and molecular modeling study.
J Med Chem 42, 4604–4613.
60 Japelj B, Pristovsek P, Majerle A & Jerala R (2005)
Structural ... tech-
niques for screening and identifying ligand binding to
protein receptors. Angew Chem Int Ed 42, 864–890.
64 Wang W, Sass HJ, Za
¨
hringer U & Grzesiek S (2008)
Structure and dynamics of
13
C,
15
N-labeled...
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Báo cáo sinh học: " Imperfect DNA mirror repeats in the gag gene of HIV-1 (HXB2) identify key functional domains and coincide with protein structural elements in each of the mature proteins" doc
Ngày tải lên :
18/06/2014, 18:20
... protein coding DNA and that different levels of symmetry are
associated with different functional aspects of the gene and its protein. The interaction between
IMRs and protein structure and function ... property
of protein coding DNA and that different levels of symme-
try are associated with different functional aspects of gene
and protein. The interaction between DNA and protein
structure and function ... because its protein motifs and
structural components are well documented.
Results: There is a highly specific relationship between IMRs and structural and functional aspects
of the Gag polyprotein....
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Tài liệu Báo cáo khoa học: Multifunctional host defense peptides: Antimicrobial peptides, the small yet big players in innate and adaptive immunity docx
Ngày tải lên :
18/02/2014, 06:20
... antimicrobial activity and inmuno-
modulatory functions of antimicrobial peptides, the
skin plays a major and active role in the onset and
development of an immune response to injury and
microbial insult. ... recently.
Human a-defensins and b-defensins induce the release
of histamine and prostaglandin D
2
in a G -protein
phospholipase C-dependent manner [62] [71]; HPN1–3
bind C1q and activate the classic ... clearance and
removal of debris. Other cells, such as T-lymphocytes,
secrete cytokines and chemokines that will further
activate macrophages and induce inflammation and
vasodilatation, and enhance...
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Tài liệu Báo cáo khoa học: Prion protein library of recombinant constructs for structural biology docx
Ngày tải lên :
18/02/2014, 08:20
... prion protein
plasmid library; prion protein structural
biology; recombinant prion proteins;
transmissible spongiform encephalopathies
Correspondence
K. Wu
¨
thrich, Institute of Molecular Biology
and ... cellular form of mPRP (accession number AAA39997) and truncated forms
and designed variants thereof, and of mouse doppel and Shadoo protein constructs. Protein accession numbers refer to the NCBI database
(http://www.ncbi.nlm.nih.gov). ... 51 prion protein constructs from bank vole, cat,
cattle, chicken, dog, elk, ferret, frog, fugu, horse, human, pig, sheep, turtle,
and wallaby, and for 113 mouse prion protein constructs and variants
thereof,...
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Tài liệu Báo cáo khoa học: The Mycobacterium tuberculosis membrane protein Rv2560 ) biochemical and functional studies pdf
Ngày tải lên :
18/02/2014, 16:20
... preimmune and post-third inoculation sera against a
M. tuberculosis sonicate (lanes 1, 2 and 5) and a membrane frac-
tion (lanes 3 and 4). Lanes 1 and 3, absence of recognition of
M. tuberculosis proteins ... subunit-based, multiepitope, anti-TB
vaccine candidate.
Rv2560 is a proline- and glycine-rich transmembrane
protein, and may thus be classified as a simple
sequence protein (SSP). This type of sequence ... for
U937 proteins specifically cross-linked with radiolabelled peptide
11024. Lanes 1 and 2, U937 cells; lanes 3 and 4, HepG2 cells;
lanes 1 and 3, total radiolabelled peptide binding; lanes 2 and...
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Tài liệu Báo cáo khoa học: The role of the ESSS protein in the assembly of a functional and stable mammalian mitochondrial complex I (NADH-ubiquinone oxidoreductase) pptx
Ngày tải lên :
19/02/2014, 16:20
... fraction.
Immunochemical assays and antibodies
Mitochondrial protein samples (between 50 and 100 lg)
were separated by SDS/PAGE and BN/PAGE and trans-
ferred t o I mmobilon-P (0.2 l) membranes. Anti-HA and
anti-porin ... prokaryotes and eukaryotes. It is
shown here that the ESSS protein is another essential
protein for the formation of a functional complex I in
mammals. The null mutants can be complemented with
ESSS proteins ... anti-Rieske
protein ( complex III), and a nti-SDHC (complex II).
3268 P. Potluri et al. (Eur. J. Biochem. 271) Ó FEBS 2004
The role of the ESSS protein in the assembly of a functional and stable
mammalian...
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Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx
Ngày tải lên :
06/03/2014, 11:20
... (Millipore, MA,
USA) and stored in buffer 50 mm Tris ⁄ HCl (pH 8.0).
Protein concentration
The protein concentration was determined by the method
of Sedmak and Grossberg [18], using BSA as standard.
Steady-state ... CONICET and
Agencia Nacional de Promocio
´
n Cientı
´
fica y Tecnolo
´
g-
ica. M. F. Drincovich and C. S. Andreo are mem-
bers of the Researcher Career of CONICET, and
V. A. Campos-Bermu´ dez and ... both the forward and reverse reactions (Fig. 5A).
NADH and ATP substantially inhibited the activ-
ity of E. coli Pta in both directions (Fig. 5A). On the
other hand, pyruvate and PEP displayed...
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Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx
Ngày tải lên :
07/03/2014, 03:20
... channel protein is Tic20. Structural
predictions place Tic20 within the large group of small
hydrophobic proteins with four transmembrane-
domains (e.g. including the channel proteins Tim17
and Tim23) ... the
import of preproteins. A prominent and difficult task
for future studies will therefore be to determine how
organelle metabolism and physiology influences protein
import by the Tic complex and by the ... 1175
MINIREVIEW
Protein transport in organelles: The composition,
function and regulation of the Tic complex in
chloroplast protein import
J. Philipp Benz
1,2
,Ju
¨
rgen Soll
1,2
and Bettina Bo
¨
lter
1,2
1...
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Báo cáo khoa học: C-terminal, endoplasmic reticulum-lumenal domain of prosurfactant protein C – structural features and membrane interactions ppt
Ngày tải lên :
07/03/2014, 05:20
... enve-
lope between 11 and 26 charges (m ⁄ z 4982–2108) was
observed, and for hexamers and nonamers, complete
envelopes between 20 and 35 charges (m ⁄ z 5480–3132)
and between 28 and 37 charges (m ... differential scanning calorimetry and
CD analyses indicate that membrane-bound CTC is less structurally
ordered than the unbound protein. The exposed hydrophobic surfaces and
the structural disordering ... prosurfactant protein C – structural features
and membrane interactions
Cristina Casals
1
, Hanna Johansson
2
, Alejandra Saenz
1
, Magnus Gustafsson
2,3
, Carlos Alfonso
4
,
Kerstin Nordling
2
and Jan...
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Báo cáo khoa học: Activation of nematode G protein GOA-1 by the human muscarinic acetylcholine receptor M2 subtype Functional coupling of G-protein-coupled receptor and G protein originated from evolutionarily distant animals doc
Ngày tải lên :
07/03/2014, 11:20
... These results indicate that M
2
in the GOA-1 fusion protein is functional, and the
ligand-binding properties agree with that of the Ga
i1
fusion protein.
Activation of GOA-1 by muscarinic agonists
Agonist-bound ... M
2
subtype
Functional coupling of G -protein- coupled receptor and G protein
originated from evolutionarily distant animals
Masaomi Minaba
1
, Susumu Ichiyama
2
, Katsura Kojima
3
, Mamiko Ozaki
4
and ... prepared an
M
2
mutant::GOA-1 fusion protein and directly assessed
the muscarinic-ligand-dependent activation of GOA-1.
Results
Expression of M
2
::GOA-1 fusion protein
The human M
2
receptor is...
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Báo cáo khoa học: Fowlicidin-3 is an a-helical cationic host defense peptide with potent antibacterial and lipopolysaccharideneutralizing activities ppt
Ngày tải lên :
07/03/2014, 11:20
... antimicrobial
defensins, cathelicidins, and eosinophil-derived neuro-
toxin in host defense. Annu Rev Immunol 22, 181–215.
6 McPhee JB & Hancock RE (2005) Function and thera-
peutic potential of host defence peptides. ... MN, USA) and tested
individually against fowlicidin-1 and fowlicidin-3. The
MICs were determined by a standard broth microdilution
assay as recommended by the Clinical and Laboratory
Standards ... secondary -structural elements, including regular and
distorted a-helix, regular and distorted b-sheet, turns, and
unordered structures, were analyzed using the program
selcon3 [26].
NMR spectroscopy and...
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Báo cáo khoa học: Intermodule cooperativity in the structure and dynamics of consecutive complement control modules in human C1r Structural biology docx
Ngày tải lên :
15/03/2014, 23:20
... ellipsoidal protein. CCP structures are highly
variable, with the invariant parts comprising mainly the
b-strands. The loops between strands B and C, known
as the ‘hypervariable loop‘, D and E, E and ... modules.
Thus, these loops are strong candidates for binding sites
of other complement and ⁄ or regulatory proteins. The
large insertion between E and F strands in C1r CCP2 is
atypical in CCP modules; ... (MASP-1 ⁄ 3,
MASP-2 and C1s).
Modules are often defined as domains (i.e. autono-
mous folding and functional units) that occur in
diverse proteins. Thus, constructs containing domains
and their combinations...
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Báo cáo khoa học: The impact of G-protein-coupled receptor hetero-oligomerization on function and pharmacology pptx
Ngày tải lên :
16/03/2014, 22:20
... specificity, and consequently in
determining the receptor fate and b-arrestin-mediated
MAPK activation.
As discussed above for G-proteins, the mechanism
and the stoichiometry by which GPCR and b-arrestin
interact ... homo-oligomer(s).
Function and pharmacology of hetero-oligomers R. Maggio et al.
2942 FEBS Journal 272 (2005) 2939–2946 ª 2005 FEBS
Effect of hetero-oligomerization on G -protein
coupling and function
To ... receptors can
influence the coupling of each other and determine the
ultimate function of the complex.
Keywords
bivalent ligand; G -protein; mitogen-activated
protein kinase (MAPK); oligomerization;
b-arrestin
Correspondence
R....
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Polysaccharides - structural diversity and functional diversity
Ngày tải lên :
23/05/2014, 13:14
... as a function
of a pair / and w. / and w can take any value between À180j
and +180j. The most likely conformation is expected to
have the lowest potential energy. For example, 38 pairs of /
and ... spectra of curdlan hydrate (A) and gel (B–D), observed by CP/MAS (A, D), by broadband decoupling (B),
and by MAS (C).
Progress in Structural Characterization of Functional Polysaccharides 23
Copyright ... polysaccharides:
1. Progress in structural characterization. The structural analysis may offer the most fundamental knowledge to
understand the functions of polysaccharides, but the diversity and irregularity...
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