condensation reaction of ethanol and formic acid
low - level detection of ethanol and h2s with temperature - modulated wo3 nanoparticle gas sensors
... transients. After calibration of the sensor for low concentrations of ethanol and H2S, it was possible to detect as little as 200 ppb of ethanol and 20 ppb of H2S (both of them dry gases) with ... between 150 and 250◦C for all further measure-ments.3.2. Calibration curves for low concentrations of ethanol and H2SAt first, different concentrations of ethanol (2, 5, 10, 20,30, 40, and 50ppm) ... 2004AbstractLow-level detection of ethanol and H2S was achieved with thermally modulated WO3nanoparticle gas sensors. Nanoparticle WO3films, with a mean grain size of ∼5 nm and a thickness of ∼20 m, were...
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Báo cáo y học: "Functional genomics analysis of low concentration of ethanol in human hepatocellular carcinoma (HepG2) cells. Role of genes involved in transcriptional and translational processes"
... genomics of low concentrations of ethanol in HepG2 cells. (A) Reported interactions of each ethanol- regulated gene studied. (B) Indirect interaction of ITGB4, COBRA1 and ANK3 with ethanol, as ... concentrations of ethanol (100 mM) induced the expression of genes involved in the metabolism of ethanol [16]. In addition, the metabolism of ethanol results in an increased production of toxic ... or the ingestion of ethanol induces a completely different pattern due to the reported ethanol- induced expression and activation of cytokines and chemokines in monocytes and macrophages (including...
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Tài liệu Báo cáo khoa học: Exposure of IgG to an acidic environment results in molecular modifications and in enhanced protective activity in sepsis doc
... preventsbrisk changes in the levels of components of inflamma-tion, coagulation, and other pathways [21]. The admini-stration of a single dose of 500 mgặkg)1 of themodied IVIg had significant ... Breeding Farm of the Bulgarian Academy of Sciences. The experimentalprotocols were approved by the Animal Care Commission of the Institute of Microbiology, in accordance withNational and European ... CRP and low-pH buffer-exposed IVIgwas 69.1 lm. The absence of detectable binding ornegligible responses precluded the estimation of reli-able values of kinetic parameters for the native and the...
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Tài liệu Báo cáo khoa học: Purification and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx
... amida-tion, hydroxylation of Pro, Val or Lys, c-carboxylation of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different ... structure of conomarphinwas similar to those of other conotoxin cDNAs. ThecDNA-encoded precursor of conomarphin consisted of a conserved M-superfamily signal peptide of 25 resi-dues, a proregion of ... sidechain of Lys9, Hyp10 and D-Phe13 of cono-marphin is shown in stick mode, and theside chain of L-Phe13 in L-Phe13-conomar-phin is shown as a gray stick.Y. Han et al. AD-amino acid- containing...
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Tài liệu Báo cáo khóa học: Active-site residues and amino acid specificity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx
... Asn210 and Ala276are important for the second-half reaction and are part of the active-site of CoaB; these residues may be directlyinvolved in binding of one of the substrates, the amino acid cysteine ... 4Â-phosphopantothenic acid, CTP and cysteine. The synthesis of PPC occurs in two half reactions starting with the formation of 4Â-phosphopantothenoyl-CMP (activation of the carboxyl group of 4Â-phosphopantothenate). ... synthesis of 4Â-phosphopantothenoyl-CMP (cysteine, upper part of the gure) and of 4Â-phosphopantothenoylcysteine (+ cysteine, lower part of the figure) by the mutant His-CoaB proteins R206Q and A276V...
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Tài liệu Báo cáo khoa học: The SCO2299 gene from Streptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain pdf
... PIPES as an internal standard, and 2 lgofprotein samples in a final volume of 100 lL. The reaction mixture was incubated for 10 min at 37 °C and transferredto 400 lL cold methanol. The contents ... Biology(Keio University)’ and a grant from New Energy and Industrial Technology Development Organization(NEDO) of the Ministry of Economy, Trade and Industry of Japan (Development of a TechnologicalInfrastructure ... Rv2228c (orMT2287) of Mycobacterium tuberculosis;Mle, ML1637 of Mycobacterium leprae;Mav, MAP1980c of Mycobacterium avium;Nfa, nfa16400 of Nocardia farcinica, and Tfu,Tfus02000308 of Thermobifida...
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Tài liệu Báo cáo khoa học: Proton transfer in the oxidative half-reaction of pentaerythritol tetranitrate reductase Structure of the reduced enzyme-progesterone complex and the roles of residues Tyr186, His181 and His184 pdf
... architec-ture of OYE1 and PETN reductase, and (ii) geometryfor the binding of steroid compounds and 2-cyclohe-xen-1-one [2,19]. The structure of the Y186F enzyme isessentially identical to that of wild-type ... C. Bruce2, Andrew W. Munro1,*, David Leys1,† and Nigel S. Scrutton1,†1 Department of Biochemistry, University of Leicester, UK2 CNAP, Department of Biology, University of York, UKPentaerythritol ... 2-cyclohexen-1-one. The H181A and H184A enzymes retain activity in reductive and oxidative half-reactions, and thus do not play an essential role in catalysis. Ligand binding and catalysis is not substantially...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... producingtransformant and was used for the purification of GALA.Growth of this transformant and subsequent purification of the enzyme were performed as described in Materials and methods. Purity of the preparation ... to facilitate purification of GALA. The enzyme has a molecular mass of 48.5 kDa and a pH optimum between 4 and 4.5. Incubations of ara-binogalactans of potato, onion and soy with GALA resultedinitially ... Maldi-TOF MS analysis of the hydrolysis of arabinogalactans by GALA. Three different arabinogalactans were used: potato (A), onion (B), and soy (C). Spectra were taken after 15 min (1) and 2 h (2) of...
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Tài liệu Báo cáo khoa học: A point mutation in the ATP synthase of Rhodobacter capsulatus results in differential contributions of DpH and Du in driving the ATP synthesis reaction pptx
... absence of Du.(C)Theratioofthe best-fitting functions and of the data points of wild-type overmutant are plotted for data in the presence (d)andabsence(m)ofDu.1988 P. Turina and B. A. Melandri ... synthase of Rhodobacter capsulatusresults in differential contributions of DpH and Du in drivingthe ATP synthesis reaction Paola Turina and B. Andrea MelandriDepartment of Biology, Laboratory of ... uncoupler- and oligomycin-sensitive and can be attributed to the ATPsynthase. This initial rate has been measured as a function of DpH in the presence and absence of Du for both mutant and wild-type...
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Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
... of nucleotides (Table 1).The rate of CTP synthesis was dramatically influenced bythe presence of GTP, and 20 and 41-fold increases in kcatwere obtained with GTP concentrations of 0.1 mM and 1mM, ... thebasis of the kinetic constants in Table 1, and those obtainedfor the glutaminase reaction in the presence of 0.1 mMeach of UTP and ATP-cS (data not shown). Apparently, thevalue of Ka, ... activation of theglutaminase activity in the presence of 0.1 mM(open circles) or 1 mM(closedcircles)eachofUTPandATP-cS. (C) GTP activation of CTPsynthesis at 0.1 mMeach of UTP and ATP.4776...
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Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc
... amino acids (i.e. Q, N, S, and T) and an increase incharged amino -acid residues (i.e. K, E, and R) inhyperthermophilic proteins. As S and T can catalyze thedeamination and backbone cleavage of ... Thermal unfolding of apo-BLXI in the presence and absence of metals followed by DSC. See Materials and methods forexperimental details.Fig. 8. Distribution of the N 1 Q (A), Q (B), and N (C) contents ... and precipitation temperatures.The lower stability and lower Ea of inactivation of BLXIin the presence of Mg2ỵreect the fact that the enzymebinds Mg2ỵwith lower specicity (the Kactof...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25