concepts in molecular biology
Methods in molecular biology vol 1590 stem cell banking concepts and protocols
... same principles are applied to clinical repositories, since the operating principles are in alignment However, in the clinical setting, additional requirements may need to be incorporated into ... Banking Initiative (ISCBI): raising standards to bank on In Vitro Cell Dev Biol Anim 46:169–172 Healy L, Young L, Stacey GN (2011) Stem cell banks: preserving cell lines, maintaining genetic integrity, ... guidelines and principles in stem cell banking, such as the International Society for Stem Cell Research (ISSCR) Guidelines for the conduct of hESC research [5], International Stem Cell Banking Initiative...
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basic cell culture protocols methods in molecular biology - cheryl d. helgason, cindy l. miller
... of cell lines using DNA fingerprinting and cytogenetic evaluation have shown a high incidence (approx 15%) of false cell lines observed among cell lines obtained directly from original investigators ... the case of Plasmocin; and (3) the use of a combination therapy applying the two antibiotics minocycline (tetracycline) and tiamulin (macrolide) in alternating cycles (BM-Cyclin) (4) (see Fig and ... enabling every researcher using human cell lines to easily verify cell line identity Key Words: Authentication; cross-contamination; PCR; DNA fingerprinting; false cell lines; VNTR; AmpFLP Introduction...
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cell cycle control mechanisms and protocols methods in molecular biology - tim humphrey, gavin brooks
... (a G2 cyclin) (reviewed in ref 20) Cyclins bind to Cdc28/Cdc2, forming an active complex, which is associated with histone H1 kinase activity In order to bind, cyclins recognize a binding motif ... ubiquitin-activating enzymes (E1), ubiquitin-conjugating enzymes (E2 or UBC), and ubiquitin–protein ligases (E3) Multiubiquitin chains are formed on lysine side chains on the target protein, which ... control, suggesting that mechanisms controlling entry into mitosis in budding yeast are more generally conserved (31) 5.2 Cyclin-Dependent Kinase Inhibitors CDK-cyclin activity can also be inhibited...
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data analysis in molecular biology and evolution - xuhua xia
... may find this book exactly what you have been looking for Acknowledgement added in the second printing Perhaps nothing is more gratifying than preparing one’s first book for the second printing, ... available, one using the Windows Installer and other using the conventional installation method The former is preferred You are strongly advised to follow the “Using Windows Installer” link to install ... it installs the Windows Installer setup.ini: the file that tells setup.exe the name of your msi file to install Either InstMsiA.exe (for Windows 95/98) or InstMsiW.exe (for Windows NT) After installation,...
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methods in molecular biology, v.474. nanostructure design. methods and protocols, 2008, p.268
... computer modeling in biotechnology These include silicon bionanodevices, carbon nanotube-biomolecular systems, lipoprotein assemblies, and protein engineering of gas-binding proteins, such as ... foldon domain In the second, as well as in the protein with the foldon at its N-terminal end, the two domains were joined without incorporating the linker sequence The chimeric proteins with the ... Coomassie blue staining Electrophoresis was carried at 4C The + symbol indicates boiling in loading buffer containing 2% SDS for prior to loading in the gel Lane 7, lysate of noninduced bacteria...
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methods in molecular biology, v.303. nanobiotechnology protocols, 2005, p.242
... bridging protein consisting of the immunoglobulin G (IgG)-binding β2 domain of streptococcal protein G modified by genetic fusion with the positively charged leucine zipper interaction domain (PG-zb), ... Series: Methods in molecular biology (Clifton, N.J.) ; 303 TP248.25.N35N35 2005 660.6 dc22 2005000473 Preface Increasingly, researchers find themselves involved in discipline-spanning science that ... protein avidin A genetically engineered maltose-binding protein appended with the charged leucine zipper (MBP-zb) was used as a purification tool in conjunction with both types of bridging proteins...
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Excluded volume effects in molecular biology and extracellular matrix biochemistry biophysical considerations and molecular modeling
... influences Intra-cellular Trafficking 6.4 Macromolecular Crowding on Protein-folding and Stability 6.5 Macromolecular Crowding Effects on Protein-Aggregation 6.6 Macromolecular Crowding Effects on in ... frequently increasing the binding strength by at least an order of magnitude (Wilf & Minton, 1981) 6.4 Macromolecular Crowding on Protein-folding and Stability Crowding influences protein stability ... polypeptide chains in nanopores has been used to mimic Macromolecular Crowding Several reports of confinement hint at biologic equivalence of confinement with Crowding In an interesting study (Purohit,...
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Methods in molecular biology vol 1540 hepatitis b virus methods and protocols
... establishing pharmacologic substances blocking initial viral binding, infection, and subsequent viral spread Lack of information about key cellular factors involved in the initial steps of HBV infection ... composed of two tubulin isoforms that have similar structure Both tubulin have a GTP binding domain in N terminus, one central domain and the C terminus part, which is implicated in interaction with ... in mind for data interpretation, especially when aiming at quantification of early infection markers including cccDNA In this chapter, we describe the methods covering preparation of virus, infection,...
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Methods in molecular biology vol 1541 cancer cytogenetics methods and protocols
... following day Incubate in 37 °C % CO2 incubator overnight Add 100 μL of working thymidine in the next morning Incubate for further 5–7 h prior harvesting Proceed with cell harvest (see Subheading ... place in a slide box and store at −20 °C to keep the slides fresh 3.11 G-Banding Using Pancreatin and Protocol Wright Giemsa/Giemsa Stain Prepare the following coplin jars in sequence for staining: ... harvesting procedure For synchronized culture, uridine and fluorodeoxyuridine are added as blocking reagents and released by thymidine on the following day Harvesting steps of the cultures involved...
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Methods in molecular biology vol 1542 mycotoxigenic fungi methods and protocols
... levels in foods and feeds of some selected mycotoxins according to the provisional maximum TDI, used as a guideline for controlling contamination by mycotoxins, and preventing and reducing toxin ... mycotoxin contamination of foods and feeds Main Aspergillus Mycotoxins The main mycotoxins produced by species belonging to Aspergillus genus are aflatoxins (B1, B2, G1, G2); ochratoxin A; fumonisins ... enzyme activity Inhibition of these two steps seems to be linked, and both are inhibited in vivo in tentoxin-sensitive plant species and not affected in insensitive species Tentoxin was also responsible...
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Methods in molecular biology vol 1545 exosomes and microvesicles methods and protocols
... Flinders Centre for Innovation in Cancer, School of Medicine, Flinders University, South Australia, Australia MichaelZ.Michael Flinders Centre for Innovation in Cancer, School of Medicine, Flinders ... In contrast, the irradiation intensity (I0) is only linearly linked to the intensity of Rayleigh scattering A large difference in the refractive index of the surrounding medium and the illuminated ... researching EVs As with other volumes in the Methods in Molecular Biology series, the notes sections at the end of each methods chapter give invaluable insight into the methods and provide information...
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Methods in molecular biology vol 1546 multiplex biomarker techniques methods and applications
... proteins Insulin Adrenals Spleen Clotting Cascade Testosterone Progesterone Insulin Pancreas Transport proteins Cytokines Interleukins Gonads Bone marrow Fig Peripheral and central signaling molecules ... exhibit high insulin levels since antipsychotic drugs can induce metabolic side effects such as insulin resistance and weight gain Interestingly, the weight gain appears to be linked to antipsychotic ... of the interleukins, cytokines, transport proteins, and clotting cascade are not listed individually for presentation reasons See text for more detail The finding of circulating changes in molecules...
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Methods in molecular biology vol 1549 proteome bioinformatics
... chemistry labels only the cysteine residues of the proteins whereas the biotin group aids in the capture of cysteine-containing peptides specifically based on biotin–avidin affinity This greatly reduces ... heavy amino acids which was used during the growth of the two cell population [20] The commonly chosen amino acids to achieve effective labeling are leucine, lysine, methionine, and arginine [16] ... proteins and identifying ligands to which they bind is an important step towards elucidating protein functions and advancement in X-ray crystallographic techniques has contributed to increasing...
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Methods in molecular biology vol 1531 type 3 secretion systems methods and protocols
... Amino acids: mM L-alanine, mM L-isoleucine, mM L-valine, mM L-leucine, mM L-phenylalanine, mM L-tyrosine, mM L-methionine, mM L-arginine HCl, 10 mM L-proline, 10 mM L-glutamic acid, mM L-lysine ... in infection Initially in infection SPI-1 effectors cause phagocytosis of the bacteria into epithelial cells and also cause an increase in inflammatory mediators and fluid movement into the intestine ... is routinely used as a donor strain in conjugal mating experiments Being a lambda lysogen carrying the pir gene, the strain can also maintain pDM4 plasmid replication Moreover, the strain harbors...
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Methods in molecular biology vol 1533 plant genomics databases methods and protocols
... Protein and Plant Gene Research, College of Life Sciences and Center for Bioinformatics, Peking University, Beijing, China Chapter Ensembl Plants: Integrating Tools for Visualizing, Mining, and ... Center for Bioinformatics, Peking University, Beijing, China Trupti Joshi • Department of Molecular Microbiology and Immunology, Medical Research Office School of Medicine, Informatics Institute, ... http://www.ebi.ac.uk/training/online/course/ensemblfilmed-browser-workshop – ● Two Ensembl browsing courses http://www.ebi.ac.uk/training/online/course/ensemblfilmed-api-workshop – The API training course...
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Methods in molecular biology vol 1534 oncogene induced senescence methods and protocols
... such as integrin β1 [21] and plasminogen inhibitor-1 (PAI-1) [22] and secreted factors such as insulin-like growth factorbinding proteins (IGFBPs) [23] and interleukin-6 (IL-6) (reviewed in ref ... of Senescence In Vivo Most of the analyses described so far have been performed mainly in cultured cells Studying OIS in vivo is hindered by many factors, including heterogeneity in responses ... cell plating Cells should always be plated in duplicate for experimental replicates SA-βgal staining should be performed in the evening Staining will become visible by h, but overnight incubation...
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Methods in molecular biology vol 1535 bacterial pathogenesis methods and protocols
... N-terminal sequencing or MS/MS 3.2 Screening Bacterial Isolates for Binding In order to screen bacteria for binding of a specific host protein, the protein of interest is first labeled with 125Iodine ... DRaCALA spots, one indicating binding (top) and another indicating no binding (bottom) The locations of protein (P), ligand (L), and ligand bound to protein (P·L), are indicated in the cartoon The ... If binding is detected in all lysates, there is likely the presence of a nonspecific binding protein in the lysate This can be tested by using a lysate containing the vector only If the binding...
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Methods in molecular biology vol 1537 oral biology molecular techniques and applications 2nd edition
... hepatitis, HIV infection, or smoking Ask subjects to refrain from eating, drinking, smoking, or oral hygiene procedures for at least 1 h prior to collection Instruct subjects to rinse the mouth ... Protein/ Peptide Determination Bicinchoninic acid (BCA) micro-protein assay reagents, Protein Assay Kit (PIERCE, Rockford, IL), a modified Lowry’s protein assay [28] Bovine serum albumin standard ... using a number of kits including Qiagen RNeasy Micro or Qiagen RNeasy Mini Kits using a centrifuga- Salivary Diagnostics Using Purified Nucleic Acids tion followed by pelleting step to obtain...
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Methods in molecular biology vol 1571 biosensors and biodetection methods and protocols, volume 1
... enabling continuous monitoring Miniaturization: Increasingly, biosensors are being miniaturized for incorporation into equipment for a wide variety of applications including clinical care, food and ... containing 10 μg/mL of the detector mAb for h incubation The fiber probe is finally rinsed with the PBS and followed by dipping in a 0.2 M glycine PBS solution for 10 to deactivate the remaining ... Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, China MEI LI Natural Products Research Center, Chengdu Institution of Biology, Chinese...
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